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Your search keyword '"Lactobacillus rhamnosus LA68"' showing total 4 results
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4 results on '"Lactobacillus rhamnosus LA68"'

1. Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration.

Author

Dimitrijevic, Rajna, Ivanovic, Nevena, Mathiesen, Geir, Petrusic, Vladimir, Zivkovic, Irena, Djordjevic, Brizita, and Dimitrijevic, Ljiljana

Subjects
LACTOBACILLUS rhamnosus, IMMUNE system, DRUG administration, CYTOKINES, PROBIOTICS, FLOW cytometry, LABORATORY mice
Abstract

Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-γ, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-γ and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations. [ABSTRACT FROM PUBLISHER]

Published
2014
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2. Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68

Author

Ljiljana Dimitrijević, Sladjana Sobajic, Irena Zivkovic, Brizita Djordjevic, Rajna Minić, Ivana Djuricic, and Nevena Ivanovic

Subjects
C57BL/6, medicine.medical_specialty, Chromatography, Gas, Lactobacillus strain, Biology, liver, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Lactobacillus rhamnosus, Internal medicine, medicine, fatty acid composition, Palmitoleic acid, Animals, Pathological, 030304 developmental biology, Brain Chemistry, 0303 health sciences, Lacticaseibacillus rhamnosus, Probiotics, Fatty Acids, food and beverages, Brain, Lactobacillus rhamnosus LA68, Organ Size, biology.organism_classification, 3. Good health, Mice, Inbred C57BL, Endocrinology, chemistry, Liver, Dietary Supplements, Arachidonic acid, Female, Fatty acid composition, 030217 neurology & neurosurgery, Bacteria, Food Science
Abstract

Recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. There are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. This study was designed to test the effects of a particular Lactobacillus strain, Lactobacillus rhamnosus LA68 on fatty acid composition of the liver and the brain of C57BL/6 mice in the absence of an underlying pathological condition. Female mice were supplemented with live L. rhamnosus LA68 bacteria for the duration of 1 month. Serum biochemistry was analyzed and liver and brain fatty acid composition was assessed by gas-liquid chromatography. Significant changes in liver and brain fatty acid composition were detected. In the liver tissue we detected an increase in palmitoleic acid (p = 0.038), while in the brain compartment we found an increase in palmitic (p = 0.042), stearic (p = 0.017), arachidonic acid (p = 0.009) and docosahexaenoic acid (p = 0.004) for control versus experimental group. These results show discrete changes caused by LA68 strain consumption. Even short duration of administration of LA68 influences the fatty acid composition of the host which adds to the existing knowledge about Lactobacillus host interaction, and adds to the growing knowledge of metabolic intervention possibilities.

Published
2014

3. Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68

Author

Ivanović, Nevena, Minić, Rajna, Đuričić, Ivana, Dimitrijević, Ljiljana, Šobajić, Slađana, Živković, Irena, Đorđević, Brižita, Ivanović, Nevena, Minić, Rajna, Đuričić, Ivana, Dimitrijević, Ljiljana, Šobajić, Slađana, Živković, Irena, and Đorđević, Brižita

Abstract

Recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. There are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. This study was designed to test the effects of a particular Lactobacillus strain, Lactobacillus rhamnosus LA68 on fatty acid composition of the liver and the brain of C57BL/6 mice in the absence of an underlying pathological condition. Female mice were supplemented with live L. rhamnosus LA68 bacteria for the duration of 1 month. Serum biochemistry was analyzed and liver and brain fatty acid composition was assessed by gas-liquid chromatography. Significant changes in liver and brain fatty acid composition were detected. In the liver tissue we detected an increase in palmitoleic acid (p = 0.038), while in the brain compartment we found an increase in palmitic (p = 0.042), stearic (p = 0.017), arachidonic acid (p = 0.009) and docosahexaenoic acid (p = 0.004) for control versus experimental group. These results show discrete changes caused by LA68 strain consumption. Even short duration of administration of LA68 influences the fatty acid composition of the host which adds to the existing knowledge about Lactobacillus host interaction, and adds to the growing knowledge of metabolic intervention possibilities.

Published
2015

4. Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration

Author

Vladimir Petrušić, Rajna Dimitrijević, Irena Zivkovic, Ljiljana Dimitrijević, Geir Mathiesen, Brizita Djordjevic, and Nevena Ivanovic

Subjects
Lipopolysaccharides, C57BL/6, Lipopolysaccharide, medicine.medical_treatment, Peptidoglycan, Biology, Lymphocyte Activation, serum cytokines, leucocyte population shift, Interferon-gamma, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, Lactobacillus rhamnosus, medicine, Splenocyte, Animals, Lymphocyte Count, IL-2 receptor, C57BL/6 mice, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Interleukin-6, Lacticaseibacillus rhamnosus, oral administration, Probiotics, Monocyte, Immunity, Lactobacillus rhamnosus LA68, General Medicine, Th1 Cells, biology.organism_classification, Interleukin-10, 3. Good health, Mice, Inbred C57BL, Cytokine, medicine.anatomical_structure, chemistry, Immunology, Cytokines, Female, Animal Science and Zoology, Spleen, 030215 immunology, Food Science
Abstract

Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption,Lactobacillus rhamnosusLA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-γ, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-γ and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.

Published
2014

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