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4. Induction of apoptosis in B16‐BL6 melanoma cells following exposure to electromagnetic fields modeled after intercellular calcium waves.

Author

Rain, Benjamin D., Plourde‐Kelly, Adam D., Lafrenie, Robert M., and Dotta, Blake T.

Subjects
ELECTROMAGNETIC fields, COMPUTATIONAL electromagnetics, CALCIUM, CANCER cells, NUCLEAR fragmentation, APOPTOSIS, CELL communication
Abstract

Exposure to time‐varying electromagnetic fields (EMF) has the capacity to influence biological systems. Our results demonstrate that exposure to time‐varying EMF modeled after the physiological firing frequency of intercellular calcium waves can inhibit proliferation and induce apoptosis in malignant cells. Single exposure of B16‐BL6 cells to a Ca2+ EMF for 40 min reduced the number of viable cells by 50.3%. Cell imaging with acridine orange and ethidium bromide dye revealed substantial cellular apoptosis, preapoptotic cells, nuclear fragmentation, and large spacing between cells in the Ca2+ EMF condition when compared to the control condition. The ability of Ca2+ EMF to influence the proliferation and survival of malignant cells suggests that exposure to specific EMF may function as a potential anticancer therapy. [ABSTRACT FROM AUTHOR]

Published
2024
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33. Experimental Demonstration That Aharanov-Bohm Phase Shift Voltages In Optical Coupler Circuits of Tuned Patterned Magnetic Fields Is Critical for Inhibition of Malignant Cell Growth

Author

Karbowski, Lukasz M., Murugan, Nirosha J, Lafrenie, Robert M, Persinger, Michael A, Karbowski, Lukasz M., Murugan, Nirosha J, Lafrenie, Robert M, and Persinger, Michael A

Abstract

The physical processes by which specific point duration magnetic fields affect aberrant expressions of living matter may involve non-classical mechanisms.The Aharanov-Bohm voltage for a quantum of energy that is convergent with the quotient of the protons magnetic moment to its charge multiplied by the viscosity of water at homeostatic temperatures applied across the distance of O-H bonds in conjunction with its phase modulation is about ±4.3 V. Application of frequency shifting, temporally-patterned magnetic fields produced by 3 ms point durations at average intensities of ~28 mG (that are equivalent to Nernst thresholds for plasma membranes) generated through optocoupler light emitting diodes produced the strongest inhibition of malignant cells growth when the pre-coupler value for the circuit maintenance was ±4.3 V compared to increments of voltage below or above this value. Spatial expansion of the effective zone for growth diminishment also occurred with this pre-voltage level. These results indicate that phase modulation of the electrons mediating cellular molecular pathways may be central to the etiology and potential treatment of malignant cells but not for normal cells dynamics. Consideration of quantum effects rather than classical electromagnetic theory may be a more effective strategy for impeding the physical bases for the molecular pathways that define malignant cells.

Published
2016

40. Uncaria tomentosa (Willd. ex Schult.) DC (Rubiaceae) Sensitizes THP-1 Cells to Radiation-induced Cell Death.

Author

Allen, Lisa, Buckner, Alison, Buckner, Carly A., Cano, Pablo, and Lafrenie, Robert M.

Subjects
UNCARIA tomentosa, CELL death, PHYSIOLOGICAL effects of ionizing radiation, JANUS kinases, CELL proliferation, CYCLIN E, PHOSPHORYLATION, ANTINEOPLASTIC agents
Abstract

Background: Uncaria tomentosa (Willd. ex Schult.) DC (Rubiaceae), known as Cat's Claw or Uña de gato, is a traditionally used medicinal plant native to Peru. Some studies have shown that U. tomentosa can act as an antiapoptotic agent and enhance DNA repair in chemotherapy-treated cells although others have shown that U. tomentosa enhanced apoptosis. Objective: To determine if treatment with U. tomentosa can significantly enhance cell death in THP-1 cells exposed to ionizing radiation. Materials and Methods: THP-1 monocyte-like cells were treated with ethanolic extracts of U. tomentosa in the presence or absence of bacterial lipopolysaccharide and then exposed to ionizing radiation. Cell proliferation was assessed by MTT and clonogenic assays and the effects on cell cycle measured by flow cytometry and immunoblotting. Changes in cell signaling were determined by immunoblotting and cytokine ELISA and activation of apoptosis measured by caspase activation and DNA fragmentation analysis. Results: Treatment of THP-1 cells with U. tomentosa had a small effect on cell proliferation. However, when the U. tomentosa-pretreated cells were also subjected to 5-9 Gy ionizing radiation, they showed a significant decrease in cell proliferation and increased cellular apoptosis as measured by DNA fragmentation and caspase activation. Treatment with U. tomentosa also decreased the expression of Cyclin E and Cyclin B, key regulators of normal cell cycle progression, and decreased the phosphorylation of various stress-activated, cell survival proteins including p38, ERK, and SAP/JNK kinase. Conclusions: These results suggest that U. tomentosa could be useful in enhancing cell death following anticancer therapies including ionizing radiation. [ABSTRACT FROM AUTHOR]

Published
2017
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