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Although mesophotic coral ecosystems account for approximately 80% of coral reefs, they remain largely unexplored due to their challenging accessibility. The acoustic richness within reefs has led scientists to consider passive acoustic monitoring as a reliable method for studying both altiphotic and mesophotic coral reefs. We investigated the relationship between benthic invertebrate sounds (1.5–22.5 kHz), depth, and benthic cover composition, key ecological factors that determine differences between altiphotic and mesophotic reefs. Diel patterns of snaps and peak frequencies were also explored at different depths to assess variations in biorhythms. Acoustic recorders were deployed at 20 m, 60 m, and 120 m depths across six islands in French Polynesia. The results indicated that depth is the primary driver of differences in broadband transient sound (BTS) soundscapes, with sound intensity decreasing as depth increases. At 20–60 m, sounds were louder at night. At 120 m depth, benthic activity rhythms exhibited low or highly variable levels of diel variation, likely a consequence of reduced solar irradiation. On three islands, a peculiar peak in the number of BTS was observed every day between 7 and 9 PM at 120 m, suggesting the presence of cyclic activities of a specific species. Our results support the existence of different invertebrate communities or distinct behaviors, particularly in deep mesophotic reefs. Overall, this study adds to the growing evidence supporting the use of passive acoustic monitoring to describe and understand ecological patterns in mesophotic reefs. [ABSTRACT FROM AUTHOR]

Mesophotic Coral Ecosystems remain largely unexplored. The aim of this study was to determine how the acoustic fish biodiversity varied depending on the depth and the type of island in six Polynesian islands. The link between benthic cover and fish sound diversity was established. In most islands, acoustic fish α-diversity decreased between 20 and 60 m but not between 60 and 120 m. Fish sound types community composition was more driven by depth, likely due to benthic coral cover differences, than by the type of island. These results show fish sounds exhibit a bathymetric stratification and can reflect different habitat features. It opens perspectives in the monitoring of mesophotic coral ecosystems using passive acoustics. [ABSTRACT FROM AUTHOR]

Aim: Revealing how diversity varies across the depth gradient is key for understanding the role of mesophotic coral ecosystems in the functioning of coral reefs. We quantitatively examined how alpha and beta generic diversity of scleractinian coral assemblages vary across a wide depth gradient for coral reefs. Location: Sixteen sites in eight islands of three archipelagos in French Polynesia. Methods: We studied generic diversity patterns of scleractinian corals, as derived from the analysis of photo‐quadrats, across the seafloor from shallow to lower mesophotic depths (6–120 m) and on a wide geographic scale. Our sampling considered quantitative coral cover to explore the patterns of alpha and beta components of diversity across depth and horizontal space. Results: We show that in French Polynesia, mesophotic coral ecosystems host higher alpha and beta generic diversity than shallow reefs despite decreasing coral cover with depth. The variation of coral genus richness across the depth gradient is mainly driven by a mid‐domain effect with a peak at 40 m depth. At the same time, we found that the differences in coral genera across islands (spatial beta‐diversity) increased steadily along the depth gradient. Main conclusions: Our findings report the first quantitative results of coral cover and diversity from mesophotic coral ecosystems in French Polynesia and also present one of the few existing studies to examine the broad breadth of the mesophotic depth gradient. We demonstrate that mesophotic depths can host unexpectedly high generic richness of scleractinian coral assemblages. At the same time, we showed that increasing depth increases the differences in generic diversity composition across islands, whereas shallow reefs are similar in between. While a single island could conserve shallow regional biodiversity, mesophotic depths containing the richest diversity require site‐specific measures, suggesting that considering these mesophotic depths in conservation is necessary to maintain regional diversity. [ABSTRACT FROM AUTHOR]

This correction notice is for an article titled "Invertebrate sounds from photic to mesophotic coral reefs reveal vertical stratification and diel diversity" published in the journal Oecologia. The correction addresses an error in the author list, where the institutional author 'Under The Pole Consortium' was incorrectly placed at the end instead of the 5th original position. The original article has been corrected. The authors of the article are Xavier Raick, Éric Parmentier, Cédric Gervaise, David Lecchini, G Bardout, J Fauchet, A Ferucci, F Gazzola, G Lagarrigue, J Leblond, E Marivint, A Mittau, N Mollon, N Paulme, E Périé-Bardout, R Pete, S Pujolle, G Siu, Gonzalo Pérez-Rosales, Héloïse Rouzé, Frédéric Bertucci, and Lucia Di Iorio. [Extracted from the article]

The symbiosis between scleractinian corals and photosynthetic algae from the family Symbiodiniaceae underpins the health and productivity of tropical coral reef ecosystems. While this photosymbiotic association has been extensively studied in shallow waters (<30 m depth), we do not know how deeper corals, inhabiting large and vastly underexplored mesophotic coral ecosystems, modulate their symbiotic associations to grow in environments that receive less than 1% of surface irradiance. Here we report on the deepest photosymbiotic scleractinian corals collected to date (172 m depth), and use amplicon sequencing to identify the associated symbiotic communities. The corals, identified as Leptoseris hawaiiensis, were confirmed to host Symbiodiniaceae, predominantly of the genus Cladocopium, a single species of endolithic algae from the genus Ostreobium, and diverse communities of prokaryotes. Our results expand the reported depth range of photosynthetic scleractinian corals (0–172 m depth), and provide new insights on their symbiotic associations at the lower depth extremes of tropical coral reefs. [ABSTRACT FROM AUTHOR]

This study assessed in vitro apical leakage in root canals filled with gutta-percha and Ketac-Endo using different dentin conditioning and filling techniques. The root canals of 63 single-rooted teeth were instrumented and randomly divided into the following four groups: group A canals were irrigated with 25% tannic acid and filled with Ketac-Endo and a standardized gutta-percha cone; group B canals were irrigated with 1% NaOCl and filled as in group A; group C canals were irrigated with 25% tannie acid filled with Ketac-Endo and a chloroform-softened gutta-percha cone adapted to the canal walls; group D canals were irrigated with 1% NaOCl and filled with Grossman's sealer and laterally condensed gutta-percha. After storage for 72 hours at 37 ° C and 100% humidity, the gutta-percha was removed to a level 4 mm short of the working length, using a Peeso reamer. Teeth were immersed in India ink for 48 hours and cleared, and the coronal extent of dye penetration examined under a binocular dissecting microscope at X 15 magnification. The mean value of leakage for group A was 0.86 mm, for group b 2.23 mm, for group C 2.34 mm, and for group D 0.56 mm. Turkey's multiple comparison test disclosed significant differences (P <0.05) between group A and group B and C, and between group D and groups B and C. Differences between groups A and D, as well as between groups B and C, were not significant. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate post space preparation difficulties in canals filled with Ketac Endo and with Grossman's sealer. Sixty-three single-rooted teeth were prepared using a step-back technique and were divided into four groups: group 1--canals were irrigated with 25% tannic acid and filled with Ketac Endo and a single-cone technique; group 2--canals were irrigated with 1% sodium hypochlorite and fillings done as in the previous group; group 3--canals were irrigated with 25% tannic acid and filled with Ketac Endo and one gutta-percha cone previously adapted with chloroform; and group 4--canals were irrigated with 1% sodium hypochlorite and filled with Grossman's sealer and a lateral condensation technique. After 72 h, canals post space were prepared using Peeso drills. The time required was measured in seconds and analyzed by Tukey multiple comparison test (level 5%). Post space preparation time for groups 1 to 3 was longer than for group 4 and longer for group 1 than for groups 2 to 4.