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Dynamic visualization of the morphology of membrane-bound organelles offers useful insights for studying various intracellular activities. Fluorescent probes with superior specificity and photostability are desirable for long-term tracking of these processes. In this work, we present the design and synthesis of an a-cyanostilbene derivative, abbreviated as ASCP, with the aggregation-induced emission (AIE) characteristic, and its application in cell imaging. ASCP can simultaneously label mitochondria and nucleolus in live cells with distinct fluorescence, which is demonstrative of a single molecule with dualcolour organelle imaging.

An atom-economical and straightforward polymerization method to generate functional isocoumarin-containing polymers was developed in this work. The oxidative polycoupling of 4,4'-(1,2-diphenyl-1,2-ethenylene)dibenzoic acid and 1,6-bis[4-(phenylethynyl)phenoxy]hexane proceeds efficiently in dimethylformamide under nitrogen or air in the presence of [Cp*RhCl2]2 and a catalytic amount of Cu(OAc)2·H2O at 120 °C for 24 h, generating a polymer with a high molecular weight of up to 42 900 in a high yield of up to 92.9%. An isocoumarin framework forms in situ during the polymerization from readily accessible and inexpensive monomers. The resulting polymer possesses good thermal stability, optical transparency and film-forming ability. Its thin film exhibits high and UV-tunable refractive indices (n =1.9697–1.6507) in a wide wavelength region of 390–890 nm. A two-dimensional fluorescent photopattern can be readily fabricated by irradiating its thin film under UV light through a copper mask. Due to the incorporation of tetraphenylethene units in the monomer, the polymer obtained is weakly emissive in solution but it emits intensely when aggregated, demonstrating a phenomenon of aggregation-induced emission.

The development of an efficient transition metal-free polymerization route has been an active research topic in polymer science owing to its low synthetic cost and decreased metal residue and hence elevated material performance in the products. In this work, we report a new such method for constructing conjugated polydiynes based on the organic reaction of 1-haloalkyne. In the presence of potassium iodide, the polymerizations of 1,2-bis[4-(iodoethynyl)phenyl]-1,2-diphenylethene (1), 1,4-bis(2-iodoethynyl)benzene (2) and 4,4'-bis(2-iodoethynyl)-1,1'-biphenyl (3) proceed smoothly in N,N-dimethylformamide at 120 °C under nitrogen, producing P1–3 consisting of alternate aromatic and 1,3-diyne moieties in moderate to satisfactory yields. While P1 possesses good solubility in common organic solvents, P2 and P3 are insoluble due to their rigid structures. All the polymers are thermally stable, losing 5% of their weight at a high temperature of up to 352 °C. A homogeneous film of P1 can be readily obtained by spin- coating its solution, which shows high and UV-tunable refractive index (n = 2.1125–1.7747) in a wide wavelength range (400–900 nm). A well-defined fluorescent photopattern can be generated by UV irradiation of the polymer film through a copper mask. This work thus opens a new avenue for constructing conjugated polymers with advanced functionalities.

A new near-infrared AIE luminogen (TPE-AC) with high specificity, good biocompatibility and excellent photostability is designed and synthesized for lipid droplet (LD) imaging in cells. TPE-AC can monitor the process of LD accumulation in cells, thus making it potential for the diagnosis of early-stage fat-related diseases.

In this work, a morpholine-functionalized aggregation-induced emission luminogen (AIEgen), AIE-LysoY, is reported for lysosomal imaging and autophagy visualization. To attain outstanding imaging contrast, AIE-LysoY is equipped with excited state intramolecular proton transfer (ESIPT) characteristic. AIE-LysoY provides a new platform for lysosome visualization with good biocompatibility, large Stokes shift, superior signal-to-noise ratio, and high photostability.

Chromophores containing olefinic double bonds are the core components of many important luminogen systems that show the novel photophysical effect of aggregation-induced emission (AIE). The role and extent of E–Z isomerization (EZI) of the double bond in affecting the solution emissions of the AIE luminogens (AIEgens), however, have not been fully understood. In this work, we verified the occurrence of EZI in the dilute solutions of TPE-cored AIEgens by NMR spectroscopy using elaborate experimental procedures. We further designed a TPE-fluorescein adduct to quantify that EZI plays a minor role whereas intramolecular rotation plays a major role in the emission quenching processes of the AIEgen solutions. This study fills the gap in the research on the restriction of the intramolecular rotation (RIR) mechanism for the AIE effect and provides a useful tool for the mechanistic investigation of photoluminescence processes.

采用示差扫描量热法（DSC）、一维（1D）、二维（2D）广角X-射线衍射（WAXD）和偏光显微镜（PLM）等研究手段对聚{11-[（4′-正庚氧基-4-联苯基）羰基]氧-1-十一炔}（PA-9,7）的本体相转变和相结构进行研究,并采用分子动力学方法对相结构进行模拟.结果表明,样品的相转变为近晶B相（SmB）近晶A相（SmA）各向同性态（Iso）.在近晶B相中,侧链在层状结构中排列成具有六次对称性的准长程有序结构。

In this work, several new compounds containing tetraphenylene (TPE) units and dimesitylboron groups were synthesized through Sonogashira coupling reaction. The compounds showed the aggregation induced emission (AIE), emitted the different color and gave the relatively high quantum yield of fluorescence in solid state. Two of the compounds demonstrated the prospective application in data storage, and one molecule also displayed the potential value in biological fluorescent cell imaging.