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13 results on '"Landi, Laure"'

4. Sensitivity of embryos to substratum quality from sturgeon’s spawning grounds

Author

Rochard, Eric, Delage, Nicolas, Morin, Bénédicte, Landi, Laure, Gesset, Charline, Le Barh, Romaric, Clérandeau, Christelle, Jatteau, Philippe, Cachot, Jérôme, Ecosystèmes aquatiques et changements globaux (UR EABX), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), and Rochard, eric

Subjects
[SDE] Environmental Sciences, [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

5. Assessing the toxicity of sediments using the medaka embryo-larval assay and 2 other bioassays

Author

Barhoumi, Badreddine, Clérandeau, Christelle, Landi, Laure, Pichon, Anaïk, Le Bihanic, Florane, Poirier, Dominique, Anschutz, Pierre, Budzinski, Hélène, Driss, Mohamed ridha, Cachot, Jérôme, Laboratory of Heteroatom Organic Chemistry, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Tunisia, and Laboratory EPOC, UMR CNRS 5805, University of Bordeaux, Talence Cedex, France

Subjects
animal structures, embryonic structures, fungi, [SDE]Environmental Sciences, [CHIM]Chemical Sciences
Abstract

International audience; Sediments are sinks for aquatic pollutants, and analyzing toxicity in such complex matrices is still challenging. To evaluate the toxicity of bioavailable pollutants accumulated in sediments from the Bizerte lagoon (Tunisia), a novel assay, the medaka embryo–larval assay by sediment contact, was applied. Japanese medaka (Oryzias latipes) embryos were incubated in direct contact with sediment samples up to hatching. Lethal and sublethal adverse effects were recorded in embryos and larvae up to 20 d postfertilization. Results from medaka embryo–larval assay were compared with cytotoxicity (Microtox®), genotoxicity (SOS chromotest), and pollutant content of sediments. The results highlight differences in the contamination profile and toxicity pattern between the different studied sediments. A significant correlation was shown between medaka embryo–larval assay by sediment contact and SOS chromotest responses and concentrations of most organic pollutants studied. No correlation was shown between pollutant levels and Microtox. According to the number of sediment samples detected as toxic, medaka embryo–larval assay by sediment contact was more sensitive than Microtox, which in turn was more sensitive than the SOS chromotest; and medaka embryo–larval assay by sediment contact allowed sediment toxicity assessment of moderately polluted sediments without pollutant extraction and using an ecologically realistic exposure scenario. Although medaka embryo–larval assay by sediment contact should be tested on a larger sample set, the results show that it is sensitive and convenient enough to monitor the toxicity of natural sediments.

Published
2016
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6. Monitoring the state of the spawning ground for future return of Acipenser sturio in the Gironde

Author

Delage, Nicolas, Cachot, J., Jatteau, Philippe, Larcher, T., Morin, Brice, Landi, Laure, Le Barh, Romaric, Rochard, Eric, Irstea Publications, Migration, Ecosystèmes aquatiques et changements globaux (UR EABX), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDE] Environmental Sciences, [SDE]Environmental Sciences
Abstract

International audience; Cette présentation synthétise les résultats des effets des contaminants organiques et minéraux présents sur les frayères et évalue la tolérance oxy-thermique des embryons et jeunes larves

Published
2016

7. Ecologie et Ecotoxicologie

Author

Geffard, Alain, Tales, Évelyne, Le Pichon, Céline, Mathieu, Aurélia, Zahm, Amandine, Slawson, Deborah, Albert, Marie-Bernadette, Girondin, Mathieu, Roy, Mathieu, Chevalier, Romain, Beslagic, Sarah, Delaigue, Olivier, Belliard, Jérôme, Barjhoux, Iris, Anschutz, Pierre, Anzil, Adriana, Autret, Armelle, Ayrault, Sophie, Berthe, Thierry, Bimbot, Maya, Bordier, Louise, Budzinski, Hélène, Cachot, Jérôme, Charron, Laetitia, Chaumot, Arnaud, Clérandeau, Christelle, Corzani-Euzebe, Giovanni, Dedourge-Geffard, Odile, Faburé, Juliette, Fechner, Lise, François, Adeline, Geffard, Olivier, George, Isabelle, Huteau, Viviane, Jaffal, Ali, Labadie, Pierre, Landi, Laure, Lebrun, Jérémie, Lévi, Yves, Munoz, Gabriel, Noury, Patrice, Oziol, Lucie, Peluhet, Laurent, Petit, Fabienne, Poirier, Dominique, Quéau, Hervé, Servais, Pierre, Uher, Emmanuelle, and Urien, Nastassia

Published
2015
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8. Effects of substrate quality of spawning grounds on European sturgeon (Acipenser sturio) embryo and larvae developement

Author

Delage, Nicolas, Clerandeau, Christelle, Landi, Laure, Jatteau, Philippe, Rochard, Eric, Cachot, Jérôme, Irstea Publications, Migration, Ecosystèmes aquatiques et changements globaux (UR EABX), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDE] Environmental Sciences, GARONNE, [SDE]Environmental Sciences, DORDOGNE
Abstract

National audience; Les embryons et larves d'esturgeon européen ont été exposés aux substrats de 2 frayères situées sur la Dordogne et la Garonne. Les individus exposés au substrat de La Réole (Garonne) présentent une augmentation du taux de malformation, une altération des capacités physiologique et natatoire.

Published
2014

11. Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects

Author

Larcher, Thibaut, Perrichon, Prescilla, Vignet, Caroline, Ledevin, Mireille, Le Menach, Karyn, Lyphout, Laura, Landi, Laure, Clerandeau, Christelle, Lebihanic, F., Menard, Dominique, Burgeot, Thierry, Budzinski, Helene, Akcha, Farida, Cachot, J., Cousin, Xavier, Larcher, Thibaut, Perrichon, Prescilla, Vignet, Caroline, Ledevin, Mireille, Le Menach, Karyn, Lyphout, Laura, Landi, Laure, Clerandeau, Christelle, Lebihanic, F., Menard, Dominique, Burgeot, Thierry, Budzinski, Helene, Akcha, Farida, Cachot, J., and Cousin, Xavier

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All PAH fractions caused preneoplastic and neoplastic disorders in long-term-exposed animals. Target tissues were almost exclusively of epithelial origin, with the bile duct epithelium being the most susceptible to chronic exposure to all PAH fractions, and with germ cells being the second most responsive cells. Significantly higher incidences of neoplasms were observed with increasing PAH concentration and exposure duration. The most severe carcinogenic effects were induced by dietary exposure to HO compared to exposure to LO or PY (45, 30 and 7 %, respectively, after 9 to 10 months of exposure to an intermediate concentration of PAHs). In contrast, earliest carcinogenic effects were detected as soon as 3 mpf after exposure to LO, including the lowest concentration, or to PY. PAH bioactivation and genotoxicity in blood was assessed by ethoxyresorufin-O-deethylase activity quantification and comet and micronuclei assays, respectively, but none of these were positive. Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenotoxic events that impair survival and physiology of exposed fish.

Published
2014
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12. Development of a reference artificial sediment for chemical testing adapted to the MELA sediment contact assay.

Author

Bihanic, Florane, Perrichon, Prescilla, Landi, Laure, Clérandeau, Christelle, Menach, Karyn, Budzinski, Hélène, Cousin, Xavier, and Cachot, Jérôme

Subjects
SEDIMENTS, ORYZIAS latipes, FISH embryos, FISH larvae, CHEMICAL reagents, POLLUTANTS
Abstract

Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo-larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial sediment was tested using a model polycyclic aromatic hydrocarbon (PAH), fluoranthene. The sediment was then validated with two other model PAHs, benz[ a]anthracene and benzo[ a]pyrene. Various developmental end points were recorded, including survival, embryonic heartbeat, hatching delay, hatching success, larval biometry and abnormalities. The final artificial sediment composition was set at 2.5 % dry weight (dw) Sphagnum peat, 5 % dw kaolin clay and 92.5 % dw silica of 0.2- to 0.5-mm grain size. In contrast with natural sediments, the chemical components of this artificial matrix are fully defined and readily identifiable. It is totally safe for fish embryos and presents relatively high sorption capacities for hydrophobic compounds. Studies with other hydrophobic and metallic contaminants and mixtures should be performed to further validate this artificial sediment. [ABSTRACT FROM AUTHOR]

Published
2014
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13. Assessing the toxicity of sediments using the medaka embryo-larval assay and 2 other bioassays.

Author

Barhoumi B, Clérandeau C, Landi L, Pichon A, Le Bihanic F, Poirier D, Anschutz P, Budzinski H, Driss MR, and Cachot J

Subjects
Animals, Biological Assay methods, Larva drug effects, Lethal Dose 50, Multivariate Analysis, Mutagens analysis, Oryzias genetics, SOS Response, Genetics drug effects, Seawater chemistry, Toxicity Tests, Tunisia, Water Pollutants, Chemical analysis, Embryo, Nonmammalian drug effects, Environmental Exposure analysis, Geologic Sediments chemistry, Mutagens toxicity, Oryzias embryology, Water Pollutants, Chemical toxicity
Abstract

Sediments are sinks for aquatic pollutants, and analyzing toxicity in such complex matrices is still challenging. To evaluate the toxicity of bioavailable pollutants accumulated in sediments from the Bizerte lagoon (Tunisia), a novel assay, the medaka embryo-larval assay by sediment contact, was applied. Japanese medaka (Oryzias latipes) embryos were incubated in direct contact with sediment samples up to hatching. Lethal and sublethal adverse effects were recorded in embryos and larvae up to 20 d postfertilization. Results from medaka embryo-larval assay were compared with cytotoxicity (Microtox®), genotoxicity (SOS chromotest), and pollutant content of sediments. The results highlight differences in the contamination profile and toxicity pattern between the different studied sediments. A significant correlation was shown between medaka embryo-larval assay by sediment contact and SOS chromotest responses and concentrations of most organic pollutants studied. No correlation was shown between pollutant levels and Microtox. According to the number of sediment samples detected as toxic, medaka embryo-larval assay by sediment contact was more sensitive than Microtox, which in turn was more sensitive than the SOS chromotest; and medaka embryo-larval assay by sediment contact allowed sediment toxicity assessment of moderately polluted sediments without pollutant extraction and using an ecologically realistic exposure scenario. Although medaka embryo-larval assay by sediment contact should be tested on a larger sample set, the results show that it is sensitive and convenient enough to monitor the toxicity of natural sediments. Environ Toxicol Chem 2016;35:2270-2280. © 2016 SETAC., (© 2016 SETAC.)

Published
2016
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