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3. Skin protective and regenerative effects of RM191A, a novel superoxide dismutase mimetic

Author

Shariev, Artur, primary, Menounos, Spiro, additional, Laos, Alistair J., additional, Laxman, Pooja, additional, Lai, Donna, additional, Hua, Sheng, additional, Zinger, Anna, additional, McRae, Christopher R., additional, Casbolt, Llewellyn S., additional, Combes, Valery, additional, Smith, Greg, additional, Hung, Tzong-tyng, additional, Dixon, Katie M., additional, Thordarson, Pall, additional, Mason, Rebecca S., additional, and Das, Abhirup, additional

Published
2021
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4. Skin protective effects of RM191A, a novel superoxide dismutase mimetic

Author

Shariev, Artur, primary, Laos, Alistair J., additional, Lai, Donna, additional, Hua, Sheng, additional, Zinger, Anna, additional, McRae, Christopher R., additional, Casbolt, Llewellyn S., additional, Combes, Valery, additional, Hung, Tzong-tyng, additional, Dixon, Katie M., additional, Thordarson, Pall, additional, Mason, Rebecca S., additional, and Das, Abhirup, additional

Published
2020
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5. Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

Author

Tjandra, Kristel C., primary, McCarthy, Nigel, additional, Yang, Lu, additional, Laos, Alistair J., additional, Sharbeen, George, additional, Phillips, Phoebe A., additional, Forgham, Helen, additional, Sagnella, Sharon M., additional, Whan, Renee M., additional, Kavallaris, Maria, additional, Thordarson, Pall, additional, and McCarroll, Joshua A., additional

Published
2019
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7. Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

Author

Tjandra, Kristel C., McCarthy, Nigel, Yang, Lu, Laos, Alistair J., Sharbeen, George, Phillips, Phoebe A., Forgham, Helen, Sagnella, Sharon M., Whan, Renee M., Kavallaris, Maria, Thordarson, Pall, and McCarroll, Joshua A.

Abstract

Medulloblastoma is a malignant brain tumor diagnosed in children. Chemotherapy has improved survival rates to approximately 70%; however, children are often left with long-term treatment side effects. New therapies that maintain a high cure rate while reducing off-target toxicity are required. We describe for the first time the use of a bacteriophage-peptide display library to identify heptapeptides that bind to medulloblastoma cells. Two heptapeptides that demonstrated high [E1-3 (1)] or low [E1-7 (2)] medulloblastoma cell binding affinity were synthesized. The potential of the peptides to deliver a therapeutic drug to medulloblastoma cells with specificity was investigated by conjugating E1-3 (1) or E1-7 (2) to doxorubicin (5). Both peptide–drug conjugates were cytotoxic to medulloblastoma cells. E1-3 doxorubicin (3) could permeabilize an in vitro blood–brain barrier and showed a marked reduction in cytotoxicity compared to free doxorubicin (5) in nontumor cells. This study provides proof-of-concept for developing peptide–drug conjugates to inhibit medulloblastoma cell growth while minimizing off-target toxicity.

Published
2020
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10. Polymersomes Prepared from Thermoresponsive Fluorescent Protein-Polymer Bioconjugates: Capture of and Report on Drug and Protein Payloads.

Author

Laos, Alistair J., Soeriyadi, Alexander H., Gooding, J. Justin, Thordarson, Pall, Wong, Chin Ken, Stenzel, Martina H., Wiedenmann, Jörg, Curmi, Paul M. G., and Marquis, Christopher P.

Subjects
*POLYMERSOMES, *FLUORESCENT proteins, *POLYMERIZATION, *SYNTHETIC biology, *FLUORESCENCE resonance energy transfer, *PHYCOERYTHRIN
Abstract

Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein-polymer bioconjugate PNIPAM- b-amilFP497 composed of thermoresponsive poly( N-isopropylacrylamide) (PNIPAM) and a green-fluorescent protein variant (amilFP497). Above 37 °C, this bioconjugate forms polymersomes that can (co-)encapsulate the fluorescent drug doxorubicin and the fluorescent light-harvesting protein phycoerythrin 545 (PE545). Using fluorescence lifetime imaging microscopy and Förster resonance energy transfer (FLIM-FRET), we can distinguish the co-encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane. [ABSTRACT FROM AUTHOR]

Published
2015
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