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8 results on '"Larsen, Mads D"'

1. Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation

Author

Zeeuw van der Laan, Eveline A.N., van der Velden, Saskia, Bentlage, Arthur E.H., Larsen, Mads D., van Osch, Thijs L.J., Mok, Juk Yee, Brasser, Giso, Geerdes, Dionne M., Koeleman, Carolien A.M., Nouta, Jan, Semple, John W., Porcelijn, Leendert, van Esch, Wim J.E., Wuhrer, Manfred, van der Schoot, C. Ellen, Vidarsson, Gestur, and Kapur, Rick

Published
2020
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2. The BNT162b2 mRNA SARS-CoV-2 Vaccine Induces Transient Afucosylated IgG1 in Naive But Not in Antigen-Experienced Vaccinees

Author

Van Coillie, Julie, primary, Pongracz, Tamas, additional, Rahmöller, Johann, additional, Chen, Hung-Jen, additional, Geyer, Chiara E., additional, van Vught, Lonneke A., additional, Buhre, Jana S., additional, Šuštić, Tonći, additional, van Osch, Thijs L. J., additional, Steenhuis, Maurice, additional, Hoepel, Willianne, additional, Wang, Wenjun, additional, Lixenfeld, Anne S., additional, Nouta, Jan, additional, Keijzer, Sofie, additional, Linty, Federica, additional, Visser, Remco, additional, Larsen, Mads D., additional, Martin, Emily L., additional, Künsting, Inga, additional, Lehrian, Selina, additional, von Kopylow, Vera, additional, Kern, Carsten, additional, Lunding, Hanna B., additional, de Winther, Menno, additional, van Mourik, Niels, additional, Rispens, Theo, additional, Graf, Tobias, additional, Slim, Marleen A., additional, Minnaar, René P., additional, Bomers, Marije, additional, Sikkens, Jonne J., additional, Vlaar, Alexander P. J., additional, van der Schoot, C. Ellen, additional, den Dunnen, Jeroen, additional, Wuhrer, Manfred, additional, Ehlers, Marc, additional, Vidarsson, Gestur, additional, and Physicians Group, Fatebenefratelli-Sacco Infectious D, additional

Published
2022
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3. High titers and low fucosylation of early human anti–SARS-CoV-2 IgG promote inflammation by alveolar macrophages

Author

Hoepel, Willianne, primary, Chen, Hung-Jen, additional, Geyer, Chiara E., additional, Allahverdiyeva, Sona, additional, Manz, Xue D., additional, de Taeye, Steven W., additional, Aman, Jurjan, additional, Mes, Lynn, additional, Steenhuis, Maurice, additional, Griffith, Guillermo R., additional, Bonta, Peter I., additional, Brouwer, Philip J.M., additional, Caniels, Tom G., additional, van der Straten, Karlijn, additional, Golebski, Korneliusz, additional, Jonkers, René E., additional, Larsen, Mads D., additional, Linty, Federica, additional, Nouta, Jan, additional, van Roomen, Cindy P.A.A., additional, van Baarle, Frank E.H.P., additional, van Drunen, Cornelis M., additional, Wolbink, Gertjan, additional, Vlaar, Alexander P.J., additional, de Bree, Godelieve J., additional, Sanders, Rogier W., additional, Willemsen, Lisa, additional, Neele, Annette E., additional, van de Beek, Diederik, additional, Rispens, Theo, additional, Wuhrer, Manfred, additional, Bogaard, Harm Jan, additional, van Gils, Marit J., additional, Vidarsson, Gestur, additional, de Winther, Menno, additional, and den Dunnen, Jeroen, additional

Published
2021
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4. Natural killer cell activation by respiratory syncytial virus-specific antibodies is decreased in infants with severe respiratory infections and correlates with Fc-glycosylation

Author

van Erp, Elisabeth A, Lakerveld, Anke J, de Graaf, Erik, Larsen, Mads D, Schepp, Rutger M, Hipgrave Ederveen, Agnes L, Ahout, Inge Ml, de Haan, Cornelis Am, Wuhrer, Manfred, Luytjes, Willem, Ferwerda, Gerben, Vidarsson, Gestur, van Kasteren, Puck B, Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, Graduate School, Landsteiner Laboratory, and AII - Inflammatory diseases

Subjects
0301 basic medicine, lcsh:Immunologic diseases. Allergy, viruses, respiratory syncytial virus, Immunology, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], antibody-dependent cell-mediated cytotoxicity, Fcmediated effector functions, Virus, antibody‐dependent cell‐mediated cytotoxicity, 03 medical and health sciences, 0302 clinical medicine, Immunology and Allergy, Medicine, Interferon gamma, NK cell, Respiratory system, General Nursing, 030304 developmental biology, Antibody-dependent cell-mediated cytotoxicity, 0303 health sciences, Innate immune system, Interferon-gamma production, biology, Respiratory tract infections, business.industry, Fc-mediated effector functions, virus diseases, NKcell, respiratory system, Fc‐mediated effector functions, interferon‐gamma, 3. Good health, 030104 developmental biology, 030220 oncology & carcinogenesis, biology.protein, fucosylation, Original Article, interferon-gamma, Antibody, business, Cell activation, lcsh:RC581-607, Natural killer cell activation, 030215 immunology, medicine.drug
Abstract

Objectives Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections in infants, and there is no vaccine available. In early life, the most important contributors to protection against infectious diseases are the innate immune response and maternal antibodies. However, antibody‐mediated protection against RSV disease is incompletely understood, as both antibody levels and neutralisation capacity correlate poorly with protection. Since antibodies also mediate natural killer (NK) cell activation, we investigated whether this functionality correlates with RSV disease. Methods We performed an observational case–control study including infants hospitalised for RSV infection, hernia surgery or RSV‐negative respiratory viral infections. We determined RSV antigen‐specific antibody levels in plasma using a multiplex immunoassay. Subsequently, we measured the capacity of these antibodies to activate NK cells. Finally, we assessed Fc‐glycosylation of the RSV‐specific antibodies by mass spectrometry. Results We found that RSV‐specific maternal antibodies activate NK cells in vitro. While concentrations of RSV‐specific antibodies did not differ between cases and controls, antibodies from infants hospitalised for severe respiratory infections (RSV and/or other) induced significantly less NK cell interferon‐γ production than those from uninfected controls. Furthermore, NK cell activation correlated with Fc‐fucosylation of RSV‐specific antibodies, but their glycosylation status did not significantly differ between cases and controls. Conclusion Our results suggest that Fc‐dependent antibody function and quality, exemplified by NK cell activation and glycosylation, contribute to protection against severe RSV disease and warrant further studies to evaluate the potential of using these properties to evaluate and improve the efficacy of novel vaccines., Respiratory syncytial virus (RSV) is a global public health concern, and vaccine development is hampered by the lack of a well‐defined correlate of protection. Here, we show that RSV‐specific antibodies from children hospitalised for severe viral respiratory infections induce significantly less natural killer (NK) cell interferon‐gamma production than those from uninfected controls. In addition, we show that NK cell activation by RSV‐specific antibodies correlates with their Fc‐glycosylation status.

Published
2020

5. Natural killer cell activation by respiratory syncytial virus-specific antibodies is decreased in infants with severe respiratory infections and correlates with Fc-glycosylation

Author

Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, van Erp, Elisabeth A, Lakerveld, Anke J, de Graaf, Erik, Larsen, Mads D, Schepp, Rutger M, Hipgrave Ederveen, Agnes L, Ahout, Inge Ml, de Haan, Cornelis Am, Wuhrer, Manfred, Luytjes, Willem, Ferwerda, Gerben, Vidarsson, Gestur, van Kasteren, Puck B, Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, van Erp, Elisabeth A, Lakerveld, Anke J, de Graaf, Erik, Larsen, Mads D, Schepp, Rutger M, Hipgrave Ederveen, Agnes L, Ahout, Inge Ml, de Haan, Cornelis Am, Wuhrer, Manfred, Luytjes, Willem, Ferwerda, Gerben, Vidarsson, Gestur, and van Kasteren, Puck B

Published
2020

6. Natural killer cell activation by respiratory syncytial virus‐specific antibodies is decreased in infants with severe respiratory infections and correlates with Fc‐glycosylation

Author

Erp, Elisabeth A, primary, Lakerveld, Anke J, additional, Graaf, Erik, additional, Larsen, Mads D, additional, Schepp, Rutger M, additional, Hipgrave Ederveen, Agnes L, additional, Ahout, Inge ML, additional, Haan, Cornelis AM, additional, Wuhrer, Manfred, additional, Luytjes, Willem, additional, Ferwerda, Gerben, additional, Vidarsson, Gestur, additional, and Kasteren, Puck B, additional

Published
2020
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7. Natural killer cell activation by respiratory syncytial virus-specific antibodies is decreased in infants with severe respiratory infections and correlates with Fc-glycosylation

Author

van Erp, Elisabeth A., primary, Lakerveld, Anke J., additional, de Graaf, Erik, additional, Larsen, Mads D., additional, Schepp, Rutger M., additional, Hipgrave Ederveen, Agnes L., additional, Ahout, Inge M. L., additional, de Haan, Cornelis A. M., additional, Wuhrer, Manfred, additional, Luytjes, Willem, additional, Ferwerda, Gerben, additional, Vidarsson, Gestur, additional, and van Kasteren, Puck B., additional

Published
2019
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8. Natural killer cell activation by respiratory syncytial virus-specific antibodies is decreased in infants with severe respiratory infections and correlates with Fc-glycosylation.

Author

van Erp EA, Lakerveld AJ, de Graaf E, Larsen MD, Schepp RM, Hipgrave Ederveen AL, Ahout IM, de Haan CA, Wuhrer M, Luytjes W, Ferwerda G, Vidarsson G, and van Kasteren PB

Abstract

Objectives: Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections in infants, and there is no vaccine available. In early life, the most important contributors to protection against infectious diseases are the innate immune response and maternal antibodies. However, antibody-mediated protection against RSV disease is incompletely understood, as both antibody levels and neutralisation capacity correlate poorly with protection. Since antibodies also mediate natural killer (NK) cell activation, we investigated whether this functionality correlates with RSV disease., Methods: We performed an observational case-control study including infants hospitalised for RSV infection, hernia surgery or RSV-negative respiratory viral infections. We determined RSV antigen-specific antibody levels in plasma using a multiplex immunoassay. Subsequently, we measured the capacity of these antibodies to activate NK cells. Finally, we assessed Fc-glycosylation of the RSV-specific antibodies by mass spectrometry., Results: We found that RSV-specific maternal antibodies activate NK cells in vitro . While concentrations of RSV-specific antibodies did not differ between cases and controls, antibodies from infants hospitalised for severe respiratory infections (RSV and/or other) induced significantly less NK cell interferon-γ production than those from uninfected controls. Furthermore, NK cell activation correlated with Fc-fucosylation of RSV-specific antibodies, but their glycosylation status did not significantly differ between cases and controls., Conclusion: Our results suggest that Fc-dependent antibody function and quality, exemplified by NK cell activation and glycosylation, contribute to protection against severe RSV disease and warrant further studies to evaluate the potential of using these properties to evaluate and improve the efficacy of novel vaccines., Competing Interests: The authors declare no conflicts of interest., (© 2020 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc.)

Published
2020
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