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Context: The hemoglobin, albumin, lymphocyte, and platelet (HALP) score is a prognostic marker in several types of malignant tumors. The prognostic value of HALP score in diffuse large B-cell lymphoma (DLBCL) remains unknown. Aim: We aimed to determine the prognostic value of baseline HALP score in DLBCL patients. Subjects and methods: We retrospectively analyzed data from 153 newly diagnosed DLBCL patients treated with R-CHOP or R-CHOP-like regimens at our university hospital center. We evaluated the significance of HALP score as a predictor of response to treatment, overall survival (OS), and event-free survival (EFS). Results: The median follow-up time for all patients was 40 months. Lower HALP score was found in patients with advanced stages of disease (P = 0.005) and in those with poor response to therapy (P = 0.004). Patients with a HALP score ≤20.8 had significantly worse 5-year OS (47.3% vs. 79.5%, P < 0.001) and 5-year EFS (40.6% vs. 76.7%, P < 0.001). These observations remained statistically significant in the multivariate Cox regression models independently of International Prognostic Index (IPI) and age. Conclusion: Lower HALP is associated with unfavorable clinicopathological characteristics of DLBCL and seems to be an IPI independent negative prognostic factor. HALP score can be easily and inexpensively applied to timely recognize DLBCL patients under higher risk of unwanted outcomes in everyday clinical practice.

Cilj: Sindrom Klippel-Trenaunay-Weber rijedak je kongenitalni sporadični poremećaj koji rezultira abnormalnim razvojem krvnih žila, mekih tkiva i kostiju. Cilj je ovog rada prikazati izazovan tijek dijagnostike i terapije u pacijentice s ovom rijetkom bolešću, gdje je izostao kožni hemangiom, inače prisutan u 98 % pacijenata i gdje se bolest javila na gornjem ekstremitetu, što je zabilježeno u tek 12,5 % slučajeva. Prikaz slučaja: U ženskog je novorođenčeta pri prvom pregledu neonatologa uočena bezbolna oteklina desne ruke i kroz kožu vidljive zadebljane vene na desnoj podlaktici i šaci. Promjene su ostale trajno prisutne uz blago ograničenu fleksiju lakatnog zgloba i prstiju desne ruke. Nakon sedam godina javili su se bolovi u desnoj ruci pa je učinjena ehotomografija mišića podlaktice i šake. Nalazi su pokazali široke venske prostore uz znakove arteriovenskih fistula, nakon čega je upućena na CT s kontrastom i flebografiju. Potvrđena je tvorba vaskularnog podrijetla, dijelom trombozirana i niske brzine protoka. U idućih šest godina tegobe su postale jače izražene, stoga je bio indiciran kirurški tretman stanja. Pacijentica je podvrgnuta ekstirpaciji varikoziteta desne ruke. Tegobe su i dalje perzistirale, stoga su učinjene magnetska rezonancija i angiografija magnetskom rezonancijom desne ruke, čiji su nalazi bili suspektni na intramuskularni hemangiom. Sedam godina kasnije učinjena je posljednja embolizacija vaskularne tvorbe te se planira nastavak operativnog liječenja. Zaključak: Sindrom Klippel-Trenaunay može se prezentirati bez kožnog hemangioma, koji je inače najčešći klinički znak. Također, znakovi sindroma mogu atipično biti prisutni na gornjim ekstremitetima., Aim: Klippel-Trenaunay-Weber syndrome is a rare congenital sporadic disorder which results in abnormal development of blood vessels, soft tissue and bones. The aim of this case report is to present a challenging course of diagnostics and treatment in patients with this rare disease, where skin hemangioma, otherwise present in 98%, was absent and disease occurred in the upper extremity, which was recorded in only 12,5% of cases. Case report: The first examination of a neonatologist in a female newborn showed painless swelling of the right arm and visible varicose veins on the right forearm and hand. The changes were permanent and with mild flexion restriction of elbow joint and right hand fingers. After seven years the pain appeared in the right arm and the echotomography of the forearm and hand muscles was done. The results showed broad veins and signs of arteriouvenous fistulas, after which the computed tomography with contrast and flebography were ordered. The presence of a structure of vascular etiology was confirmed, partially thrombosed, with slow flow. In the next six years simptoms advanced and surgical treatment was recommended. The patient has undergone varicosities extirpation. The pain persisted, therefore magnetic resonance and magnetic resonance angiography of the right arm were indicated and suspected of intramuscular hemangioma. Embolisation of vascular malformation was performed seven years later and there are plans for further surgical treatment. Conclusion: Klippel-Trenaunay syndrome may occur without cutaneous hemangioma, which is otherwise the most common clinical sign. Also, syndrome signs could atypically be found in the upper extremities.

Cilj: Klippel-Trenaunay-Weber sindrom je rijetki kongenitalni sporadični poremećaj koji rezultira abnormalnim razvojem krvnih žila, mekih tkiva i kostiju. Cilj ovog rada je prikazati izazovan tijek dijagnostike i terapije u pacijentice s ovom rijetkom bolešću, gdje je izostao kožni hemangiom, inače prisutan u 98% pacijenata i gdje se bolest javila na gornjem ekstremitetu, što je zabilježeno u tek 12, 5% slučajeva. Prikaz slučaja: U ženskog novorođenčeta je pri prvom pregledu neonatologa uočena bezbolna oteklina desne ruke i kroz kožu vidljive zadebljane vene na desnoj podlaktici i šaci. Promjene su ostale trajno prisutne uz blago ograničenu fleksiju lakatnog zgloba i prstiju desne ruke. Nakon sedam godina javili su se bolovi u desnoj ruci, po čemu je učinjena ehotomografija mišića podlaktice i šake. Nalazi su pokazali široke venske prostore uz znakove arterio-venskih fistula, nakon čega je upućena na CT s kontrastom i flebografiju. Potvrđena je tvorba vaskularnog podrijetla, dijelom trombozirana i niske brzine protoka. U narednih šest godina tegobe su postale jače izražene, stoga je bio indiciran kirurški tretman stanja. Pacijentica je podvrgnuta ekstirpaciji varikoziteta desne ruke. Tegobe su i dalje perzistirale, stoga su učinjene magnetska rezonancija i angiografija magnetskom rezonancijom desne ruke, čiji su nalazi bili suspektni na intramuskularni hemangiom. Sedam godina kasnije učinjena je posljednja embolizacija vaskularne tvorbe te se planira nastavak operativnog liječenja. Zaključak: Klippel-Trenaunay sindrom može se prezentirati bez kožnog hemangioma, koji je inače najčešći klinički znak. Također, znakovi sindroma mogu atipično biti prisutni na gornjim ekstremitetima.

In the last couple of years, video games (VG) have become largely accessible, and more and more people started playing them as a result of fun, boredom, or stress relief. They are most popular among children, teenagers, and young adults. However, in many cases instead of providing fun, they have become an addiction that obstructs them in their everyday life and sleeping schedule, resulting in poor sleep quality. The aim of this study was to investigate the frequency of VS addiction and its effect on the sleep quality of college students. This online, anonymous questionnaire study was done during one month by the use of a specially designed questionnaire, which contained Problematic Online Gaming Questionnaire Short Form (POGQSF), Sleep Quality Survey (SQS), and demographic data questions. There was a total of 230 participants currently studying at the University of Osijek. There were 42, 60% (98/230) males. Age median was 22 years with a minimum being 18 and a maximum of 44 years. 16, 5% (38/230) were classified as having problematic gaming habits according to POGQSF, acquired point median was 23 points, the minimum was 12, and the maximum was 55 points. Regarding SQS median of acquired points was 28, minimum was 13, and maximum 64 points. The study has shown that students who were classified as having problematic gaming habits according to POGQSF had poorer sleep quality p=0, 007, also female students had poorer sleep quality p=0, 014. VG addiction disturbs sleep quality in college students of the University of Osijek.

Introduction: Philadelphia-positive chronic myeloid leukemia (CML) is a clonal myeloproliferative disease driven by t(9 ; 22) (q34 ; q11) chromosomal translocation that gives rise to a druggable, constitutively active tyrosine kinase BCR-ABL1. As the disease progresses, the surviving pool of self-renewing leukemic stem cells continues to accumulate mutational events that end-up in a widespread loss of heterozygosity (LOH) and microsatellite instability (MSI). In solid cancer, these processes have been associated with treatment failure and poor prognosis, but less is known about the prevalence and impact of MSI and LOH in CML. Materials and methods: Paired buccal swabs and peripheral blood samples were collected from 10 healthy volunteers and 18 Ph(+)CML patients with one (n=13) or two (n=5) prior TKIs and evidence of complete cytogenetic response. Poly- and mononuclear leukocytes were separated via gradient centrifugation, and QlAmp DNA Blood Midi set/InstaGene Matrix were used for DNA extraction. DNA was quantified (Qubit fluorometer), and 15 somatic short tandem repeats (STR) were analyzed by capillary electrophoresis (AmpFLSTR Identifiler Plus PCR Amplification set). Results: MSI events were encountered in 4 out of 18 CML patients, including 2 individuals who switched from imatinib to second-generation TKI (nilotinib). The highest MSI occurrence was noticed in polymorphonuclears (17 %), most often within D8S1179 (n=3), D3S1358 (n=2), and D5S818 (n=2) loci. No MSI was observed in buccal swabs representing germline STR content in either group. Conclusion: MSI is unfrequent in CML, at least in the initial, therapeutically amenable stage. High-coverage whole-genome sequencing is needed to address genetic instabilities in stable and accelerated CML.

Introduction/Objectives: Schizophrenia is a chronic psychiatric disorder with a heterogeneous genetic and neurobiological background it is expressed as a combination of psychotic symptoms and cognitive dysfunctions. It`s prevalence in general population is about 1%. Symptoms of schizophrenia are categorised as positive (delusions, hallucinations and disorganized speech), negative (social with¬drawal, affective flattening, anhedonia) and cognitive. On average, patients with schizophrenia have a shorter life than the general population. Suicide is considered the main reason for shorter life in younger schizophrenia patients while cardiovascular comorbidities are main contributors in later years. Antipsychotics can relieve psychotic symptoms, but do not lead to significant improvements in social and cognitive functioning. Case report: We present a case of a 53-year old patient diagnosed with schizophrenia. The patient was hospitalised at the Clinic for Psychiatry multiple time, because of several suicide attempts. She attempted suicide in different wais, she took several pills of valproate, twice she tried to cut her throat and once she cut both of her wrists. Complete blood test results were normal, except total cholesterol (6, 24mmol/l) and LDL cholesterol (4, 26 mmol/l) which were elevated. EEG and CT scan were normal. Physically, the patient has hypertension. In therapy she takes valproate, alprasolam, risperidon, zolpidem, biperiden and intramuscular depo application of paliperidon. Before each suicide attempt the patient had severe exacerbation of negative symptoms (imperative auditory hallucination, negative thoughts such as worthless). During hospitalization the patient was included in sociotherapy programme and her pharmacological treatment was modified. Conclusions: Schizophrenia is on of the most serious psychiatric diagnoses. The patients have shorter life because of suicide in the earlier age. Antipsychotics can relive symptoms, but they will not significantly improve cognitive and social functioning and that is the reason why all patients with schizophrenia should be included in sociotherapy programmes. We presented this case report to show how devastating suicide attempts can be and how deeply they can torture a patient. After each treatment modification and sociotherapy programme the patient was stabilised and distant from suicidal thoughts.

Introduction: We aimed to compare the genetic profile of Eastern Croatian population by studying differences in short tandem repeat (STR) allele frequency between unrelated volunteers from 5 eastern Croatian counties (n=101), general Croatian population (n=195) and four neighbouring countries (Serbia (n=365), Bosnia and Herzegovina (n=1000), Hungary (n=4213) and Montenegro (n=101)). Materials and methods: Peripheral blood was sampled on FTA cards, and genomic DNA extracted with the use of Chelex method. AmpFlSTR® Identifiler Plus kit and ProFlex 3x32 well PCR system were used for the multiplex amplification of 15 STR loci including D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA. Allelic variants were identified by electrophoresis on an ABI 310 Genetic Analyser and GeneMapper ID v3.2.1 was used for allele annotation. Allelic frequencies were obtained by direct counting and the exact test of population differentiation was calculated using Markov-chain algorithm and Arlequin v3.5.2.2 software. Results: In total, 129 STR alleles were detected in the Eastern Croatian population, 8 of which qualifying as rare variants (frequency

The goal: To investigate microdifferentiation of Croatian population by studying 15 short tandem repeat (STR) loci in 101 adult, unrelated volunteers from 5 eastern Croatian counties. Materials and methods: Peripheral blood was sampled on FTA cards, and genomic DNA extracted with the use of Chelex 100 method. AmpFlSTR® Identifiler PlusTM kit and ProFlex 3x32 well PCR system were used for multiplex amplification of 15 STR loci including D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA. Allelic variants were identified by electrophoresis on an ABI 310 Genetic Analyser and GeneMapper ID v3.2.1 was used for raw data analysis. Allelic frequencies were obtained by direct counting. Departures from Hardy-Weinberg equilibrium (HWE, 1000 permutations) and the exact test of population differentiation were estimated with the STRAF 1.0.5 and Arlequin v3.5.2.2 software. Results: In total, 129 STR alleles were detected, 8 of which qualifying as rare variants (frequency

Poster is dealing with the comorbidity of psychotic symptoms and somatic symptoms in patients.

Poster is dealing with the interaction of psychosis and porphyria