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3. A Contribution to the Harmonization of Non-targeted NMR Methods for Data-Driven Food Authenticity Assessment

Author

Gallo, V, Ragone, R, Musio, B, Todisco, S, Rizzuti, A, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Pascazio, A, Cobas, C, Mari, S, Garino, C, Arlorio, M, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Barison, A, Benevelli, F, Borioni, A, Cagliani, L, Casadei, L, Marincola, F, Colson, K, Consonni, R, Costantino, G, Cremonini, M, Davalli, S, Duarte, I, Guyader, S, Hamon, E, Hegmanns, M, Lamanna, R, Longobardi, F, Mallamace, D, Mammi, S, Markus, M, Menezes, L, Milone, S, Molero-Vilchez, D, Mucci, A, Napoli, C, Rossi, M, Saez-Barajas, E, Savorani, F, Schievano, E, Sciubba, F, Sobolev, A, Takis, P, Thomas, F, Villa-Valverde, P, Latronico, M, Gallo V., Ragone R., Musio B., Todisco S., Rizzuti A., Mastrorilli P., Pontrelli S., Intini N., Scapicchio P., Triggiani M., Pascazio A., Cobas C., Mari S., Garino C., Arlorio M., Acquotti D., Airoldi C., Arnesano F., Assfalg M., Barison A., Benevelli F., Borioni A., Cagliani L. R., Casadei L., Marincola F. C., Colson K., Consonni R., Costantino G., Cremonini M. A., Davalli S., Duarte I., Guyader S., Hamon E., Hegmanns M., Lamanna R., Longobardi F., Mallamace D., Mammi S., Markus M., Menezes L. R. A., Milone S., Molero-Vilchez D., Mucci A., Napoli C., Rossi M. C., Saez-Barajas E., Savorani F., Schievano E., Sciubba F., Sobolev A., Takis P. G., Thomas F., Villa-Valverde P., Latronico M., Gallo, V, Ragone, R, Musio, B, Todisco, S, Rizzuti, A, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Pascazio, A, Cobas, C, Mari, S, Garino, C, Arlorio, M, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Barison, A, Benevelli, F, Borioni, A, Cagliani, L, Casadei, L, Marincola, F, Colson, K, Consonni, R, Costantino, G, Cremonini, M, Davalli, S, Duarte, I, Guyader, S, Hamon, E, Hegmanns, M, Lamanna, R, Longobardi, F, Mallamace, D, Mammi, S, Markus, M, Menezes, L, Milone, S, Molero-Vilchez, D, Mucci, A, Napoli, C, Rossi, M, Saez-Barajas, E, Savorani, F, Schievano, E, Sciubba, F, Sobolev, A, Takis, P, Thomas, F, Villa-Valverde, P, Latronico, M, Gallo V., Ragone R., Musio B., Todisco S., Rizzuti A., Mastrorilli P., Pontrelli S., Intini N., Scapicchio P., Triggiani M., Pascazio A., Cobas C., Mari S., Garino C., Arlorio M., Acquotti D., Airoldi C., Arnesano F., Assfalg M., Barison A., Benevelli F., Borioni A., Cagliani L. R., Casadei L., Marincola F. C., Colson K., Consonni R., Costantino G., Cremonini M. A., Davalli S., Duarte I., Guyader S., Hamon E., Hegmanns M., Lamanna R., Longobardi F., Mallamace D., Mammi S., Markus M., Menezes L. R. A., Milone S., Molero-Vilchez D., Mucci A., Napoli C., Rossi M. C., Saez-Barajas E., Savorani F., Schievano E., Sciubba F., Sobolev A., Takis P. G., Thomas F., Villa-Valverde P., and Latronico M.

Abstract

Spectroscopic non-targeted methods are gaining ever-growing importance in quality control and authenticity assessment of food products because of their strong potential for identification of specific features of the products by data-driven classifiers. One of the factors hampering the diffusion of spectroscopic non-targeted methods and data-driven classifiers is the lack of harmonized guidelines for their development and validation. In particular, to date, neither conditions to directly compare spectra recorded by different spectrometers nor studies demonstrating the statistical equivalence of the spectra are available. Among the spectroscopic analytical techniques suitable for the development of non-targeted methods, nuclear magnetic resonance (NMR) offers the unique opportunity to generate statistically equivalent signals. In this paper, the feasibility of NMR spectroscopy to generate statistically equivalent NMR signals from a number of different spectrometers was demonstrated for complex mixtures (aqueous extracts of wheat and flour) by organizing an inter-laboratory comparison involving 36 NMR spectrometers. Univariate statistics along with multivariate analysis were exploited to establish unbiased criteria for assessing the statistical equivalence of the NMR signals. The aspects affecting the signal equivalence were investigated, and possible solutions to reduce the extent of the human error were proposed and applied with satisfactory results. This study furnishes the scientific community with an appropriate and easy procedure to validate non-targeted NMR methods and provides error values to be used as a reference for future studies.

Published
2020

8. A community-built calibration system: The case study of quantification of metabolites in grape juice by qNMR spectroscopy

Author

Musio, B, Ragone, R, Todisco, S, Rizzuti, A, Latronico, M, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Di Noia, T, Acquotti, D, Airoldi, C, Assfalg, M, Barge, A, Bateman, L, Benevelli, F, Bertelli, D, Bertocchi, F, Bieliauskas, A, Borioni, A, Caligiani, A, Callone, E, Čamra, A, Cesare Marincola, F, Chalasani, D, Consonni, R, Dambruoso, P, Davalli, S, David, T, Diehl, B, Donarski, J, Gil, A, Gobetto, R, Goldoni, L, Hamon, E, Harwood, J, Kobrlová, A, Longobardi, F, Luisi, R, Mallamace, D, Mammi, S, Martin-Biran, M, Mazzei, P, Mele, A, Milone, S, Molero Vilchez, D, Mulder, R, Napoli, C, Ragno, D, Randazzo, A, Rossi, M, Rotondo, A, Šačkus, A, Sáez Barajas, E, Schievano, E, Sitaram, B, Stevanato, L, Takis, P, Teipel, J, Thomas, F, Torregiani, E, Valensin, D, Veronesi, M, Warren, J, Wist, J, Zailer-Hafer, E, Zuccaccia, C, Gallo, V, Musio, Biagia, Ragone, Rosa, Todisco, Stefano, Rizzuti, Antonino, Latronico, Mario, Mastrorilli, Piero, Pontrelli, Stefania, Intini, Nicola, Scapicchio, Pasquale, Triggiani, Maurizio, Di Noia, Tommaso, Acquotti, Domenico, Airoldi, Cristina, Assfalg, Michael, Barge, Alessandro, Bateman, Lorraine, Benevelli, Francesca, Bertelli, Davide, Bertocchi, Fabio, Bieliauskas, Aurimas, Borioni, Anna, Caligiani, Augusta, Callone, Emanuela, Čamra, Ales, Cesare Marincola, Flaminia, Chalasani, Dinesh, Consonni, Roberto, Dambruoso, Paolo, Davalli, Silvia, David, Taylor, Diehl, Bernd, Donarski, James, Gil, Ana M., Gobetto, Roberto, Goldoni, Luca, Hamon, Erwann, Harwood, John S., Kobrlová, Andrea, Longobardi, Francesco, Luisi, Renzo, Mallamace, Domenico, Mammi, Stefano, Martin-Biran, Magali, Mazzei, Pierluigi, Mele, Andrea, Milone, Salvatore, Molero Vilchez, Dolores, Mulder, Roger J., Napoli, Claudia, Ragno, Daniele, Randazzo, Antonio, Rossi, Maria Cecilia, Rotondo, Archimede, Šačkus, Algirdas, Sáez Barajas, Elena, Schievano, Elisabetta, Sitaram, Bhavaraju, Stevanato, Livio, Takis, Panteleimon G., Teipel, Jan, Thomas, Freddy, Torregiani, Elisabetta, Valensin, Daniela, Veronesi, Marina, Warren, John, Wist, Julien, Zailer-Hafer, Elina, Zuccaccia, Cristiano, Gallo, Vito, Musio, B, Ragone, R, Todisco, S, Rizzuti, A, Latronico, M, Mastrorilli, P, Pontrelli, S, Intini, N, Scapicchio, P, Triggiani, M, Di Noia, T, Acquotti, D, Airoldi, C, Assfalg, M, Barge, A, Bateman, L, Benevelli, F, Bertelli, D, Bertocchi, F, Bieliauskas, A, Borioni, A, Caligiani, A, Callone, E, Čamra, A, Cesare Marincola, F, Chalasani, D, Consonni, R, Dambruoso, P, Davalli, S, David, T, Diehl, B, Donarski, J, Gil, A, Gobetto, R, Goldoni, L, Hamon, E, Harwood, J, Kobrlová, A, Longobardi, F, Luisi, R, Mallamace, D, Mammi, S, Martin-Biran, M, Mazzei, P, Mele, A, Milone, S, Molero Vilchez, D, Mulder, R, Napoli, C, Ragno, D, Randazzo, A, Rossi, M, Rotondo, A, Šačkus, A, Sáez Barajas, E, Schievano, E, Sitaram, B, Stevanato, L, Takis, P, Teipel, J, Thomas, F, Torregiani, E, Valensin, D, Veronesi, M, Warren, J, Wist, J, Zailer-Hafer, E, Zuccaccia, C, Gallo, V, Musio, Biagia, Ragone, Rosa, Todisco, Stefano, Rizzuti, Antonino, Latronico, Mario, Mastrorilli, Piero, Pontrelli, Stefania, Intini, Nicola, Scapicchio, Pasquale, Triggiani, Maurizio, Di Noia, Tommaso, Acquotti, Domenico, Airoldi, Cristina, Assfalg, Michael, Barge, Alessandro, Bateman, Lorraine, Benevelli, Francesca, Bertelli, Davide, Bertocchi, Fabio, Bieliauskas, Aurimas, Borioni, Anna, Caligiani, Augusta, Callone, Emanuela, Čamra, Ales, Cesare Marincola, Flaminia, Chalasani, Dinesh, Consonni, Roberto, Dambruoso, Paolo, Davalli, Silvia, David, Taylor, Diehl, Bernd, Donarski, James, Gil, Ana M., Gobetto, Roberto, Goldoni, Luca, Hamon, Erwann, Harwood, John S., Kobrlová, Andrea, Longobardi, Francesco, Luisi, Renzo, Mallamace, Domenico, Mammi, Stefano, Martin-Biran, Magali, Mazzei, Pierluigi, Mele, Andrea, Milone, Salvatore, Molero Vilchez, Dolores, Mulder, Roger J., Napoli, Claudia, Ragno, Daniele, Randazzo, Antonio, Rossi, Maria Cecilia, Rotondo, Archimede, Šačkus, Algirdas, Sáez Barajas, Elena, Schievano, Elisabetta, Sitaram, Bhavaraju, Stevanato, Livio, Takis, Panteleimon G., Teipel, Jan, Thomas, Freddy, Torregiani, Elisabetta, Valensin, Daniela, Veronesi, Marina, Warren, John, Wist, Julien, Zailer-Hafer, Elina, Zuccaccia, Cristiano, and Gallo, Vito

Abstract

Nuclear Magnetic Resonance (NMR) is an analytical technique extensively used in almost every chemical laboratory for structural identification. This technique provides statistically equivalent signals in spite of using spectrometer with different hardware features and is successfully used for the traceability and quantification of analytes in food samples. Nevertheless, to date only a few internationally agreed guidelines have been reported on the use of NMR for quantitative analysis. The main goal of the present study is to provide a methodological pipeline to assess the reproducibility of NMR data produced for a given matrix by spectrometers from different manufacturers, with different magnetic field strengths, age and hardware configurations. The results have been analyzed through a sequence of chemometric tests to generate a community-built calibration system which was used to verify the performance of the spectrometers and the reproducibility of the predicted sample concentrations.

Published
2020

12. Salmonella serotypes isolated in geckos kept in seven collections in southern Italy

Author

Russo, T. P., Varriale, L., Borrelli, L., Pace, A., Latronico, M., Menna, L. F., Fioretti, A., Dipineto, L., Russo, T. P., Varriale, L., Borrelli, L., Pace, A., Latronico, M., Menna, L. F., Fioretti, A., and Dipineto, L.

Subjects
Geckos, Salmonella, Prevalence
Abstract

OBJECTIVES: Reptiles are considered an important reservoir of Salmonella species. This study evaluated the prevalence of Salmonella species in different species of gecko kept as pets in Italy. MATERIALS AND METHODS: Faecal swab samples were collected from 70 clinically healthy geckos and examined for Salmonella species by culture that were then serotyped. RESULTS: Salmonella species were isolated from 24 of 70 (34·3%) samples. Eighteen isolates expressed resistance to ceftazidime and four isolates to ampicillin. CLINICAL SIGNIFICANCE: Salmonella spp. can be isolated from apparently healthy captive gecko which should be considered as a potential source of infection for humans and other companion animals.

Published
2018

26. Multinuclear and Dynamic NMR Study of trans-[Pt(Cl)(PHCy2)2(PCy2)], [Pt(Cl)(PHCy2)3][BF4], [Pt(Cl)(PHCy2)3][Cl], trans-[Pt(Cl)(PHCy2)2{P(S)Cy2}], and trans-[Pt(Cl)(PHCy2)2{P(O)Cy2}]. Influence of Intramolecular P=O···H-P and Cl···H-P Interactions on Restricted Rotation about Pt-P Bond. X-ray Structure of trans-[Pt(Cl)(PHCy2)2{P(O)Cy2}]

Author

MASTRORILLI P., NOBILE C. F., LATRONICO M., GALLO V., ENGLERT U., SCIACOVELLI O., FANIZZI, Francesco Paolo, Mastrorilli, P., Nobile, C. F., Latronico, M., Gallo, V., Englert, U., Fanizzi, Francesco Paolo, and Sciacovelli, O.

Abstract

Dynamic NMR expts. on trans-[Pt(Cl)(PHCy2)2{P(X)Cy2}]z where X is a lone pair (1, z = 0), H (2, z = +1), S (3, z = 0), or O (4, z = 0) show that the rotation around the P(X)-Pt bond is hindered for all mols. studied, with G.thermod. ranging from 8.2 to 11.0 kcal/mol. The highest value of the series was calcd. for trans-[Pt(Cl)(PHCy2)2{P(O)Cy2}] (4) where intramol. P:OH-P interactions act as a mol. brake at room temp. Single-crystal x-ray diffraction confirms the presence of both intra and intermol. P:OH interactions in solid 4. In the case of [Pt(Cl)(PHCy2)3]Cl, multinuclear NMR anal. indicates a P-HCl- interaction in arom. or halogenated solvents which could have also a minor effect on the rotational barrier around the P(X)-Pt bond.

Published
2005

27. Performance Assessment in Fingerprinting and Multi Component Quantitative NMR Analyses

Author

Gallo, V, Intini, N, Mastrorilli, P, Latronico, M, Scapicchio, P, Triggiani, M, Bevilacqua, V, Fanizzi, P, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Benevelli, F, Bertelli, D, Cagliani, L, Casadei, L, Cesare Marincola, F, Colafemmina, G, Consonni, R, Cosentino, C, Davalli, S, De Pascali, S, D'Aiuto, V, Faccini, A, Gobetto, R, Lamanna, R, Liguori, F, Longobardi, F, Mallamace, D, Mazzei, P, Menegazzo, I, Milone, S, Mucci, A, Napoli, C, Pertinhez, T, Rizzuti, A, Rocchigiani, L, Schievano, E, Sciubba, F, Sobolev, A, Tenori, L, Valerio, M, Gallo, V, Intini, N, Mastrorilli, P, Latronico, M, Scapicchio, P, Triggiani, M, Bevilacqua, V, Fanizzi, P, Acquotti, D, Airoldi, C, Arnesano, F, Assfalg, M, Benevelli, F, Bertelli, D, Cagliani, L, Casadei, L, Cesare Marincola, F, Colafemmina, G, Consonni, R, Cosentino, C, Davalli, S, De Pascali, S, D'Aiuto, V, Faccini, A, Gobetto, R, Lamanna, R, Liguori, F, Longobardi, F, Mallamace, D, Mazzei, P, Menegazzo, I, Milone, S, Mucci, A, Napoli, C, Pertinhez, T, Rizzuti, A, Rocchigiani, L, Schievano, E, Sciubba, F, Sobolev, A, Tenori, L, and Valerio, M

Abstract

An interlaboratory comparison (ILC) was organized with the aim to set up quality control indicators suitable for multicomponent quantitative analysis by nuclear magnetic resonance (NMR) spectroscopy. A total of 36 NMR data sets (corresponding to 1260 NMR spectra) were produced by 30 participants using 34 NMR spectrometers. The calibration line method was chosen for the quantification of a five-component model mixture. Results show that quantitative NMR is a robust quantification tool and that 26 out of 36 data sets resulted in statistically equivalent calibration lines for all considered NMR signals. The performance of each laboratory was assessed by means of a new performance index (named Qp-score) which is related to the difference between the experimental and the consensus values of the slope of the calibration lines. Laboratories endowed with a Qp-score falling within the suitable acceptability range are qualified to produce NMR spectra that can be considered statistically equivalent in terms of relative intensities of the signals. In addition, the specific response of nuclei to the experimental excitation/relaxation conditions was addressed by means of the parameter named NR. NR is related to the difference between the theoretical and the consensus slopes of the calibration lines and is specific for each signal produced by a well-defined set of acquisition parameters. (Graph Presented).

Published
2015

28. Bilateral dexamethasone intravitreal implant (Ozurdex®) in a young patient with Vogt-Koyanagi-Harada disease and refractory uveitis

Author

Latronico, M. e., Rigante, Donato, Cantarini, L., Caso, F., Traversi, C., Franceschini, R., Rigante, Donato (ORCID:0000-0001-7032-7779), Latronico, M. e., Rigante, Donato, Cantarini, L., Caso, F., Traversi, C., Franceschini, R., and Rigante, Donato (ORCID:0000-0001-7032-7779)

Abstract

Vogt-Koyanagi-Harada disease (VKHD) is a multisystemic disorder characterized by granulomatous panuveitis with exudative retinal detachments, often associated with neurologic and cutaneous manifestations. Therapy relies mainly on the use of corticosteroids, administrated as oral or intravenous high-dose pulses, and immunosuppressants. However, since macular edema and retinal detachment can often be refractory to systemic therapies, intravitreal triamcinolone acetate has been used successfully. Herein, we report the first case of refractory bilateral panuveitis in a young patient with VKHD successfully treated with dexamethasone intravitreal implant.

Published
2015

29. <italic>Salmonella</italic> serotypes isolated in geckos kept in seven collections in southern Italy.

Author

Russo, T. P., Varriale, L., Borrelli, L., Pace, A., Latronico, M., Menna, L. F., Fioretti, A., and Dipineto, L.

Abstract

Objectives: Reptiles are considered an important reservoir of Salmonella species. This study evaluated the prevalence of Salmonella species in different species of gecko kept as pets in Italy. Materials and Methods: Faecal swab samples were collected from 70 clinically healthy geckos and examined for Salmonella species by culture that were then serotyped. Results: Salmonella species were isolated from 24 of 70 (34·3%) samples. Eighteen isolates expressed resistance to ceftazidime and four isolates to ampicillin. Clinical Significance: Salmonella spp. can be isolated from apparently healthy captive gecko which should be considered as a potential source of infection for humans and other companion animals. [ABSTRACT FROM AUTHOR]

Published
2018
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37. D 3.5.2 System verification

Author

Amoretti M., Fides Valero A., Latronico M., Abril Jimenez P., Furfari F., Lenzi S., Tazari M., Hamisu P., Dosis S., Anastasiadis M., and Tsangalis K.

Subjects
Ambient Assisted Living, Mobile Middleware, Context-aware Computing
Abstract

The purpose of this document is to provide the instructions for retrieving and installing the PERSONA platform (release 1.0), as a whole or component by component. Moreover the document illustrates how the PERSONA platform (release 1.0.0) has been deployed and verified in ITACA's Living Lab, in Valencia.

Published
2009

38. Randomized clinical trial on the effects of full mouth disinfection versus conventional quadrant therapy in the control of chronic periodontitis

Author

Latronico M, Russo R, Garbarino F, Rolandi C, Andrea Mascolo, and Blasi G

Subjects
Adult, Disinfection, Chronic Disease, Humans, Periodontitis
Abstract

This clinical trial investigates the effectiveness of full-mouth disinfection (FMD) versus conventional etiological therapy in patients with chronic periodontitis (CP).The therapy effectiveness was assessed by a randomized trial, performed over 20 adult periodontitis (AP) patients, divided into two groups. Patients were recruited undergoing strict inclusion/exclusion criteria. The following parameters were considered to evaluate and compare the two procedures: bleeding on probing (BOP), Plaque Index (PLI), probing depth (PD), clinical attachment level (CAL). These clinical data were collected at baseline and at three follow-ups (three months, six months and twelve months from baseline). Each parameter was averaged within each group; then statistic comparisons were performed within groups and between groups.In the test-group statistically significant improvements (P0.001) were found for all parameters between baseline and every following review. The same result was reported in the control group (with a further significant difference between first and second review). Finally, the comparison between groups did not show any difference at any time for every parameter considered.FMD outcomes are similar to those of the conventional therapy and improvements can be achieved more quickly. FMD does not cause remarkable side effects and reduces the number of therapy sessions. Some aspects about this treatment need further research: maybe FMD could give an extra reduction of bacterial load, in comparison with traditional therapy, resulting in a longer free-infection period; that could allow a decrease in the frequency of supporting periodontal treatment.

Published
2008

42. Synthesis of phosphido-bridged phosphinito platinum(I) complexes by reaction of cis-PtCl2(PHCy2)2 with oxygenated bases - Crystal structure of [(PCy2OMe)Pt(μ-PCy2)] 2(Pt-Pt)

Author

Gallo, V., Latronico, M., Mastrorilli, P., Nobile, C. F., Suranna, G. P., Ciccarella, G., Englert, U., V., Gallo, M., Latronico, P., Mastrorilli, C. F., Nobile, G. P., Suranna, Ciccarella, Giuseppe, and U., Englert

Subjects
phosphinite, bridging ligands, Platinum, phosphinites, oxygenated bases, platinum, bridging ligand
Abstract

Reaction of cis-PtCl2(PHCy2)2 (1) with oxygenated bases leads to phosphido-bridged dinuclear complexes. The product obtained using sodium hydroxide is the asymmetric Pt1 complex [(Cy2PH)Pt(μ-PCy2)(κ2P,O-μ-Cy 2PO)Pt(Cy2PH)](Pt-Pt) (2), which represents a rare example of a complex containing a Pt-Pt-P-O cycle. The reaction products between 1 and NaOR (R = Me, Et) depend on experimental conditions: lack of base results in the formation of trans-[Pt(PHCy2) Cl(μ-PCy2)]2 (3) as the main product. Using an excess of base at 50°C allowed the isolation of the symmetric PtI dimers [(PCy2OR)P(μ- PCy2)]2(Pt-Pt) (5, R = Me; 7, R = Et) containing two alkyl dicyclohexylphosphinito ligands. The asymmetric compounds [(PCy 2H)Pt(μ-PCy2)2Pt(PCy2OR)](Pt-Pt) (4, R = Me; 8, R = Et) form after work-up of the reaction of 1 with excess NaOR (8 equiv.) in toluene/methanol at room temperature. Investigations on the mechanism of formation of phosphinito PtI complexes (carried out also employing NaOtBu and NaOPh as oxygenated bases) show that the first two steps of the overall reaction are the metathesis leading to cis-Pt(OR) 2(PHCy2)2 and the subsequent formation of terminal phosphido complexes through ROH elimination. Single crystal X-ray diffraction showed that molecules of 5 are located on cristallographic inversion centres; hence their necessarily planar Pt2P2 core contains a Pt-Pt bond.

Published
2005

49. Post-natal cardiomyocytes can generate iPS cells with an enhanced capacity toward cardiomyogenic re-differentation

Author

Rizzi, R, Di Pasquale, E, Portararo, P, Papait, R, Cattaneo, P, Latronico, M, Altomare, C, Sala, L, Zaza, A, Hirsch, E, Naldini, L, Condorelli, G, Bearzi, C, ALTOMARE, CLAUDIA, SALA, LUCA, ZAZA, ANTONIO, CONDORELLI, GIANLUIGI, Bearzi, C., Rizzi, R, Di Pasquale, E, Portararo, P, Papait, R, Cattaneo, P, Latronico, M, Altomare, C, Sala, L, Zaza, A, Hirsch, E, Naldini, L, Condorelli, G, Bearzi, C, ALTOMARE, CLAUDIA, SALA, LUCA, ZAZA, ANTONIO, CONDORELLI, GIANLUIGI, and Bearzi, C.

Abstract

Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of a few embryonic transcription factors. The resulting induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack the capacity to proliferate. Here, we report that neonatal CMs can be reprogrammed to generate iPS cells that express embryonic-specific markers and feature gene-expression profiles similar to those of mouse embryonic stem (mES) cell and cardiac fibroblast (CF)-derived iPS cell populations. CM-derived iPS cells are able to generate chimeric mice and, moreover, re-differentiate toward CMs more efficiently then either CF-derived iPS cells or mES cells. The increased differentiation capacity is possibly related to CM-derived iPS cells retaining an epigenetic memory of the phenotype of their founder cell. CM-derived iPS cells may thus lead to new information on differentiation processes underlying cardiac differentiation and proliferation.

Published
2012

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