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While play-based learning is important for a high quality early education, only when teachers are confident and competent in enacting play-based learning in their everyday practice are expected benefits of play-based learning for whole-child development realized. A sample of 592 early childhood educators who assumed different job titles in Hong Kong kindergartens participated in this study. This study investigated the roles of these educators' perceptions of personal and organizational enabling factors in facilitating teachers' enactment of play-based learning and further their perceived children's whole-child development. Research Findings: These educators reported that two personal enablers (i.e. beliefs of play-based learning and self-efficacy beliefs) and two organizational enablers (i.e. teacher collaboration and home-kindergarten collaboration) were positively associated with their enactment and further enhanced their perceived whole-child development through different mechanisms. Teachers' reported enactment of play-based learning played significant mediation roles. Practice or Policy: Policy makers and change facilitators should convey clear messages and take effective measures to promote play-based learning in kindergartens. Kindergarten principals need to create a mutually supportive climate in the kindergarten to praise and appreciate teachers' good practice of play-based learning, and enhance two kinds of collaborative relationships: teacher collaboration and home-kindergarten collaboration. [ABSTRACT FROM AUTHOR]

Background: Children with craniopharyngiomas (CPs) typically suffer from a life-long chronic disease. The younger the child, the more vulnerable the maturing brain is to invasive therapies such as surgery or radiotherapy. Therefore, treatment modalities facilitating avoidance or delay of invasive therapies are beneficial for these patients. In the last decade, intracystic injection of interferon alfa-2a or alfa-2b evolved as a treatment of choice based on efficacy and minor toxicity. However, the drug is no longer available internationally. After an extensive pharmacological review, peginterferon alfa-2a was identified as the agent with closest similarity. Methods: A retrospective case series is described, including five patients treated with intracystic peginterferon alfa-2a for cystic CP according to an innovative care protocol. After initial CP cyst aspiration, peginterferon alfa-2a was injected once per week via an Ommaya reservoir for 6 weeks followed by response assessment with MRI. Results: Patients' age ranged from 4 to 54 years (four patients <12 years, one adult patient). Intracystic therapy with peginterferon alfa-2a was tolerated well by all five individuals without any major toxicities and resulted in cyst shrinkage in all of the five patients. The importance of a permeability study prior to commencing intracystic therapy became apparent in one patient who suffered from cyst leakage. Conclusions: Intracystic treatment with peginterferon alfa-2a was found to be a tolerable and efficacious treatment modality in patients with cystic CP. This experience warrants further research with a larger number of patients with measurement of long-term efficacy and safety outcomes. [ABSTRACT FROM AUTHOR]

Subject relative clauses (RCs) have been shown to be acquired earlier, comprehended more accurately, and produced more easily than object RCs by children. While this subject preference is often claimed to be a universal tendency, it has largely been investigated piecemeal and with low-powered experiments. To address these issues, this meta-analysis follows an established and rigorous scientific method to test the generalizability of the subject preference in RC acquisition by evaluating the collective evidence. While the results show a significant crosslinguistic subject preference, there is a large amount of heterogeneity in the data. The manifestation of this subject preference may not be uniform across languages, depending on typological properties such as language headedness, RC headedness, and main clause similarity. The true impact of these features, however, requires research on more typologically diverse languages. [ABSTRACT FROM AUTHOR]

Differences in the ability of opioid drugs to promote regulated endocytosis of μ-opioid receptors are related to their tendency to produce drug tolerance and dependence. Here we show that drug-specific differences in receptor internalization are determined by a conserved, 10-residue sequence in the receptor's carboxyl-terminal cytoplasmic tail. Diverse opioids induce receptor phosphorylation at serine (S)375, present in the middle of this sequence, but opioids differ markedly in their ability to drive higher-order phosphorylation on flanking residues [threonine (T)370, T376, and T379]. Multi-phosphorylation is required for the endocytosis-promoting activity of this sequence and occurs both sequentially and hierarchically, with S375 representing the initiating site. Higher-order phosphorylation involving T370, T376, and T379 specifically requires GRK2/3 isoforms, and the same sequence controls opioid receptor internalization in neurons. These results reveal a biochemical mechanism differentiating the endocytic activity of opioid drugs.

Resumptive pronouns are often regarded as a last-resort strategy for rescuing illicit long-distance dependencies. Previous work has demonstrated a facilitative role for resumptive pronouns in production as well as in comprehension, though not a grammatical option in the languages. This study examined whether the same pattern is found in Cantonese, a language that allows resumption as a legitimate relativization strategy. Young Cantonese monolingual children were tested for both comprehension and production of object relative clauses using different relativization strategies (the gap strategy vs. the resumptive pronoun strategy). Although children did not perform better on either strategy in comprehension, and never employed resumptive pronouns in their production, resumption did prevent misanalysis of prenominal relative clauses as matrix clauses, suggesting that these elements help reveal the full structure of the relative clause to the processor. The study also showed better performance on subject than on object relative clauses, suggesting that the widely observed subject--object asymmetry also applies to Cantonese.

During organogenesis, immunosurveillance, and inflammation, chemokines selectively recruit leukocytes by activating seven-transmembrane-spanning receptors. It has been suggested that an important component of this process is the formation of a haptotactic gradient by immobilization of chemokines on cell surface glycosaminoglycans (GAGs). However, this hypothesis has not been experimentally demonstrated in vivo. In the present study we investigated the effect of mutations in the GAG binding sites of three chemokines, monocyte chemoattractant protein-I/CC chemokine ligand (CCL)2, macrophage-inflammatory protein-1[beta]/ CCL4, and RANTES/CCL5, on their ability to recruit cells in vivo. These mutant chemokines retain chemotactic activity in vitro, but they are unable to recruit cells when administered intraperitoneally. Additionally, monomeric variants, although fully active in vitro, are devoid of activity in vivo. These data demonstrate that both GAG binding and the ability to form higher-order oligomers are essential for the activity of particular chemokines in vivo, although they are not required for receptor activation in vitro. Thus, quaternary structure of chemokines and their interaction with GAGs may significantly contribute to the localization of leukocytes beyond migration patterns defined by chemokine receptor interactions.

Research has been conducted on the M3 protein which alteres host antiviral inflammatory responses. The crystal structures of this protein alone and in complex with CC chemokine MPC-1 have been investigated and the details are reported.