13 results on '"Laura Maria Francisca Kuijpers"'
Search Results
2. Enteric Fever in Cambodia: Community Perceptions and Practices Concerning Disease Transmission and Treatment
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Panha Chung, Amy Parry, Sotharith Bory, Sambunny Uk, Jan Jacobs, Laura Maria Francisca Kuijpers, Koen Peeters Grietens, Charlotte Gryseels, and Bun Sreng
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Male ,Health Knowledge, Attitudes, Practice ,BLOOD ,COMPLEX INTERVENTIONS ,Disease ,PARATYPHOID FEVER ,0302 clinical medicine ,Surveys and Questionnaires ,SEEKING BEHAVIOR ,030212 general & internal medicine ,Qualitative Research ,Public, Environmental & Occupational Health ,Family Characteristics ,Ceftriaxone ,Articles ,Middle Aged ,Anti-Bacterial Agents ,Infectious Diseases ,Social Perception ,INFECTIONS ,Female ,HEALTH ,Thematic analysis ,Cambodia ,Life Sciences & Biomedicine ,Adult ,medicine.medical_specialty ,030231 tropical medicine ,Pharmacy ,Health literacy ,03 medical and health sciences ,Virology ,Tropical Medicine ,SURVEILLANCE ,medicine ,Humans ,Medical prescription ,Typhoid Fever ,Science & Technology ,ANTIMICROBIAL RESISTANCE ,business.industry ,TYPHOID-FEVER ,Public health ,Community Participation ,Patient Acceptance of Health Care ,Salmonella typhi ,Focus group ,Family medicine ,Salmonella paratyphi A ,RISK-FACTORS ,Parasitology ,Medicine, Traditional ,Gentamicins ,business ,Qualitative research - Abstract
Enteric fever is a systemic bacterial infection in humans that is endemic in Cambodia and for which antibiotic resistance is increasingly reported. To guide public health programs, this qualitative study sought to explore community perceptions on transmission and treatment. Participant observation was carried out in hospital settings, pharmacies, and at a community level in Phnom Penh. In-depth interviews 39 and one focus group discussion were carried out with blood culture-confirmed enteric fever patients and purposively selected key informants. Informants were theoretically sampled based on initial themes identified using abductive analysis. Nvivo 11 was used for thematic coding. An urgent need to address health literacy concerning the transmission of enteric fever was identified, as lay informants did not link the disease and its symptoms to bacterial contamination of foods and drinks but rather to foods considered "bad" following humoral illness interpretations. As a result, lay informants considered recurrence of enteric fever preventable with appropriate dietary restrictions and Khmer traditional medicines. This study also reveals pluralistic health-care-seeking behavior. For initial and mild symptoms, patients preferred home treatment or traditional healing practices; limited household finances delayed treatment seeking. When symptoms persisted, patients first visited drug outlets or private practitioners, where they received a mix of nonessential medicines and one or more antibiotics often without prescription or confirmation of diagnosis. Inappropriate use of antibiotics was common and was related to diagnostic uncertainty and limited finances, factors which should be addressed during future efforts to improve the uptake of appropriate diagnostics and treatment of enteric fever. ispartof: AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE vol:99 issue:6 pages:1369-1377 ispartof: location:United States status: published
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- 2018
3. Chromogenic media for the detection of Salmonella enterica serovar Paratyphi A in human stool samples: evaluation in a reference setting
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Jan Jacobs, Annelies Post, and Laura Maria Francisca Kuijpers
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0301 basic medicine ,Microbiology (medical) ,Serotype ,Salmonella ,food.ingredient ,Chromogenic ,Salmonella enterica serovar Paratyphi A ,030106 microbiology ,Salmonella paratyphi A ,General Medicine ,Biology ,Chromogenic Compounds ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,fluids and secretions ,0302 clinical medicine ,Infectious Diseases ,food ,medicine ,Agar ,030212 general & internal medicine ,Feces - Abstract
Detection of Salmonella Paratyphi A stool carriers by conventional stool culture media is hindered by the absence of hydrogen sulphide production compared to most other Salmonella serovars. This study evaluated the detection of Salmonella Paratyphi A in stool samples using Salmonella chromogenic media compared to a conventional medium. Four chromogenic media, COMPASS Salmonella agar (Biokar Diagnostics, Beauvais, France), BBL™ CHROMagar™ Salmonella (BD Diagnostics, Erembodegem-Aalst, Belgium), Brilliance™ Salmonella agar (Oxoid Ltd., Basingstoke, UK) and Salmonella PLUS CHROMagar™ (CHROMagar, Paris, France), were compared to conventional Salmonella-Shigella agar (Oxoid Ltd.). The colony morphology of 29 freshly grown stock isolates (Salmonella and competing organisms) was assessed. The limit of detection (LOD) was also determined using saline and stool suspensions. Finally, recognizability of Salmonella Paratyphi A isolates was assessed using 20 human stool samples spiked with different concentrations of Salmonella Paratyphi A. All Salmonella Paratyphi A isolates demonstrated detectable growth with typical purple-coloured colonies that could be clearly differentiated from competing organisms on all four chromogenic media. The LOD for Salmonella Paratyphi A was 103 colony-forming units (CFU)/ml for all media, except for Brilliance agar (105 CFU/ml of stool). Salmonella Paratyphi A was easy to differentiate from competing organisms in the spiked stool samples. Colony yields improved when an enrichment step (Selenite-F broth (BD Diagnostics, Erembodegem-Aalst, Belgium)) and prolonged incubation for 48 h were performed. Chromogenic media demonstrated good specificity and a low LOD for the detection of Salmonella Paratyphi A in stool samples.
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- 2018
4. Diagnostic accuracy of the InBiOS AMD rapid diagnostic test for the detection of Burkholderia pseudomallei antigen in grown blood culture broth
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Thong Phe, Chentha San, M. Peeters, Laura Maria Francisca Kuijpers, Syna Teav, Kristien Mortelmans, Panha Chung, Jan Jacobs, Chhundy Phe, and Hua Lin
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0301 basic medicine ,Burkholderia pseudomallei ,Melioidosis ,MELIOIDOSIS ,Diagnostic accuracy ,Rapid diagnostic test ,0302 clinical medicine ,Medical microbiology ,EPIDEMIOLOGY ,Blood culture ,Immunoassay ,biology ,medicine.diagnostic_test ,General Medicine ,Data Accuracy ,Infectious Diseases ,Health Resources ,Original Article ,Cambodia ,Life Sciences & Biomedicine ,Adult ,Microbiology (medical) ,medicine.medical_specialty ,030106 microbiology ,030231 tropical medicine ,GLANDERS ,Sensitivity and Specificity ,Microbiology ,03 medical and health sciences ,medicine ,Humans ,Low-resource setting ,Retrospective Studies ,Antigens, Bacterial ,Bacteriological Techniques ,Science & Technology ,IDENTIFICATION ,business.industry ,CAMBODIA ,Burkholderia pseudomallei antigen ,PHNOM-PENH ,equipment and supplies ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Culture Media ,NORTHEAST THAILAND ,Burkholderia ,Blood Culture ,Reagent Kits, Diagnostic ,business - Abstract
To assess the diagnostic and operational performance of the InBiOS AMD rapid diagnostic test (RDT) (Seattle, USA) for the detection of B. pseudomallei in grown blood culture broth. The InBiOS RDT is a lateral flow immunoassay in a strip format detecting B. pseudomallei capsular polysaccharide in culture fluids, marketed for research only. Broth of blood culture bottles (BacT/Alert, bioMérieux, Marcy L’Etoile, France) sampled in adult patients at the Sihanouk Hospital Center of HOPE, Phnom Penh, Cambodia, during 2010–2017 and stored at − 80 °C was tested. They included samples grown with B. pseudomallei (n = 114), samples with no growth (n = 12), and samples with growth of other pathogens (n = 139, among which Burkholderia cepacia (n = 5)). Diagnostic sensitivity and specificity were 96.5% [95% confidence interval (CI): 91.3–98.6%] and 100% [CI: 97.5–100%] respectively. Background clearance and line intensities were good and very good. The RDT’s test strip, not housed in a cassette, caused difficulties in manipulation and biosafety. The centrifugation step prescribed by the procedure challenged biosafety, but processing of 19 B. pseudomallei samples without centrifugation showed similar results for line intensity and background clearance, compared to centrifugation. The InBiOS RDT showed excellent accuracy for detection of B. pseudomallei in grown blood culture broth. Provided operational adaptations such as cassette housing, it has the potential to reduce time to diagnosis of melioidosis. Electronic supplementary material The online version of this article (10.1007/s10096-018-3237-3) contains supplementary material, which is available to authorized users.
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- 2018
5. An African Salmonella Typhimurium ST313 sublineage with extensive drug-resistance and signatures of host adaptation
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Derek Pickard, Simon Clare, Katherine Harcourt, Eva Heinz, Jan Jacobs, Dauly Ngbonda, Elizabeth J. Klemm, Nicole E. Wheeler, Emily A. Lees, Marie-France Phoba, Stijn Deborggraeve, Tessa De Block, Sushmita Sridhar, Barbara Barbé, Koen Vandelannoote, Dadi Falay, Octavie Lunguya, Lisette Mbuyi Kalonji, Eve L. Coomber, Laura Maria Francisca Kuijpers, Gordon Dougan, Sandra Van Puyvelde, Van Puyvelde, Sandra [0000-0001-8434-5732], Heinz, Eva [0000-0003-4413-3756], Coomber, Eve L [0000-0002-7093-2263], Lees, Emily A [0000-0003-0229-7509], Wheeler, Nicole E [0000-0003-4599-9164], Klemm, Elizabeth J [0000-0002-2175-3752], Kuijpers, Laura [0000-0002-4480-7872], Jacobs, Jan [0000-0002-8657-6777], Deborggraeve, Stijn [0000-0003-1501-5355], Apollo - University of Cambridge Repository, Coomber, Eve L. [0000-0002-7093-2263], Lees, Emily A. [0000-0003-0229-7509], Wheeler, Nicole E. [0000-0003-4599-9164], and Klemm, Elizabeth J. [0000-0002-2175-3752]
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0301 basic medicine ,Salmonella typhimurium ,Salmonella ,THP-1 Cells ,631/326/325/2482 ,General Physics and Astronomy ,Drug resistance ,Azithromycin ,medicine.disease_cause ,706/134 ,wc_269 ,Antimicrobial resistance ,ANNOTATION ,38/70 ,DISEASE ,Mice ,Plasmid ,Ciprofloxacin ,Bacterial genetics ,Drug Resistance, Multiple, Bacterial ,NONTYPHOIDAL SALMONELLA ,EPIDEMIOLOGY ,129 ,lcsh:Science ,Phylogeny ,Developing world ,Multidisciplinary ,biology ,SPECTRUM BETA-LACTAMASE ,Adaptation, Physiological ,3. Good health ,Anti-Bacterial Agents ,Multidisciplinary Sciences ,Salmonella enterica ,INFECTIONS ,Democratic Republic of the Congo ,Science & Technology - Other Topics ,64/60 ,Host adaptation ,Engineering sciences. Technology ,medicine.drug ,Plasmids ,631/114/739 ,141 ,Science ,NUCLEOTIDE ,030106 microbiology ,13/106 ,45/23 ,wa_395 ,Microbial Sensitivity Tests ,SEQUENCE ,General Biochemistry, Genetics and Molecular Biology ,Article ,Microbiology ,qw_45 ,Cell Line ,03 medical and health sciences ,medicine ,Animals ,Humans ,qw_131 ,Whole genome sequencing ,Science & Technology ,45 ,Whole Genome Sequencing ,General Chemistry ,ENTERICA ,biology.organism_classification ,Multiple drug resistance ,Mice, Inbred C57BL ,030104 developmental biology ,692/699/255/1318 ,DISCOVERY ,Biofilms ,631/326/22/1434 ,lcsh:Q ,Bacterial infection - Abstract
Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) infections are dominated by isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence of ST313 sublineage II.1 in the Democratic Republic of the Congo. Sublineage II.1 exhibits extensive drug resistance, involving a combination of multidrug resistance, extended spectrum β-lactamase production and azithromycin resistance. ST313 lineage II.1 isolates harbour an IncHI2 plasmid we name pSTm-ST313-II.1, with one isolate also exhibiting decreased ciprofloxacin susceptibility. Whole genome sequencing reveals that ST313 II.1 isolates have accumulated genetic signatures potentially associated with altered pathogenicity and host adaptation, related to changes observed in biofilm formation and metabolic capacity. Sublineage II.1 emerged at the beginning of the 21st century and is involved in on-going outbreaks. Our data provide evidence of further evolution within the ST313 clade associated with iNTS in SSA., Invasive non-typhoidal Salmonella (iNTS) infections are dominated by antibiotic resistant isolates of the sequence type (ST) 313. Here, the authors identify the ST313 sublineage II.1 in the Democratic Republic of the Congo exhibiting extensive drug resistance and genetic signatures potentially associated with host adaptation.
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- 2019
6. Diagnostic accuracy of antigen-based immunochromatographic rapid diagnostic tests for the detection of Salmonella in blood culture broth
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Chun Kham, Panha Chung, M. Peeters, Barbara Barbé, Octavie Lunguya, Laura Maria Francisca Kuijpers, Jan Jacobs, and Marie-France Phoba
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Male ,Bacterial Diseases ,0301 basic medicine ,Serotype ,Salmonella ,Salmonellosis ,Bacterial Broth Dilution ,Physiology ,lcsh:Medicine ,Bacteremia ,Pathology and Laboratory Medicine ,medicine.disease_cause ,Salmonella typhi ,Salmonella Typhi ,Chromatography, Affinity ,0302 clinical medicine ,Limit of Detection ,Medicine and Health Sciences ,Blood culture ,030212 general & internal medicine ,lcsh:Science ,Child ,Multidisciplinary ,medicine.diagnostic_test ,biology ,Salmonella paratyphi A ,Middle Aged ,Bacterial Typing Techniques ,Bacterial Pathogens ,Body Fluids ,Multidisciplinary Sciences ,Infectious Diseases ,Blood ,Salmonella Enterica ,Medical Microbiology ,Salmonella enterica ,INFECTIONS ,Child, Preschool ,Salmonella Infections ,Salmonella Typhimurium ,Democratic Republic of the Congo ,Science & Technology - Other Topics ,Female ,Pathogens ,Anatomy ,Cambodia ,Research Article ,Adult ,BACTEREMIA ,Adolescent ,Salmonella enteritidis ,Preservation, Biological ,030106 microbiology ,Serogroup ,Proof of Concept Study ,Sensitivity and Specificity ,Microbiology ,Antibiotic Susceptibility Testing ,Young Adult ,03 medical and health sciences ,Species Specificity ,Enterobacteriaceae ,Antigen ,Diagnostic Medicine ,medicine ,Humans ,Developing Countries ,Microbial Pathogens ,Cryopreservation ,Pharmacology ,Antigens, Bacterial ,Bacteriological Techniques ,Science & Technology ,Bacteria ,business.industry ,TYPHOID-FEVER ,lcsh:R ,Organisms ,Biology and Life Sciences ,GLOBAL BURDEN ,biology.organism_classification ,Culture Media ,Early Diagnosis ,Pharmacologic Analysis ,Blood Culture ,lcsh:Q ,Reagent Kits, Diagnostic ,business ,RESISTANCE - Abstract
Background In low resource settings, Salmonella serovars frequently cause bloodstream infections. This study investigated the diagnostic performance of immunochromatographic rapid diagnostic tests (RDTs), which detect Salmonella antigens, when applied to stored grown blood culture broth. Material/Methods The SD Bioline One Step Salmonella Typhi Ag Rapid Detection Kit (Standard Diagnostics, Republic of Korea), marketed for the detection of Salmonella enterica serovar Typhi (Salmonella Typhi) in stool and the Salmonella Ag Rapid Test (Creative Diagnostics, USA), marketed for the detection of all Salmonella serotypes in stool, were selected for evaluation based on a pre-test evaluation of six RDT products. The limits of detection (LOD) for culture suspensions were established and the selected RDT products were assessed on 19 freshly grown spiked blood culture broth samples and 413 stored clinical blood culture broth samples, collected in Cambodia and the Democratic Republic of the Congo. Results The LOD of both products was established as 107−108 CFU/ml. When applied to clinical blood culture broth samples, the diagnostic sensitivity and specificity of the SD Bioline RDT were respectively 100% and 79.7% for the detection of Salmonella Typhi; 94.4% (65/69) of false-positive results were caused by Salmonella Enteritidis. When considering the combined detection of Salmonella Typhi and Enteritidis (both group D Salmonella), sensitivity and specificity were 97.9% and 98.5% respectively. For Creative Diagnostics, diagnostic sensitivity was 78.3% and specificity 91.0% for all Salmonella serotypes combined; 88.3% (53/60) of false negative results were caused by Salmonella Paratyphi A. Conclusions When applied to grown blood culture broths, the SD Bioline RDT had a good sensitivity and specificity for the detection of Salmonella Typhi and Salmonella Enteritidis. The Creative Diagnostics product had a moderate sensitivity and acceptable specificity for the detection of all Salmonella serovars combined and needs further optimization. A RDT that reliably detects Salmonella Paratyphi A is needed.
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- 2018
7. Voice- and swallow-related quality of life in idiopathic Parkinson's disease
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R Vos, Bernd Kremer, Emilia Michou, Walmari Pilz, Laura Maria Francisca Kuijpers, Michel R. A. van Hooren, and Laura W. J. Baijens
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medicine.medical_specialty ,Visual analogue scale ,business.industry ,digestive, oral, and skin physiology ,Dysphagia ,humanities ,03 medical and health sciences ,0302 clinical medicine ,Otorhinolaryngology ,Quality of life ,Swallowing ,030220 oncology & carcinogenesis ,Severity of illness ,otorhinolaryngologic diseases ,Physical therapy ,medicine ,Voice Handicap Index ,medicine.symptom ,MD Anderson Dysphagia Inventory ,Prospective cohort study ,business ,030217 neurology & neurosurgery - Abstract
Objectives/Hypothesis This study explores whether changes in voice- and swallow-related QoL are associated with progression of idiopathic Parkinson's disease (IPD). Furthermore, it examines the relationship between patients' perception of both voice and swallowing disorders in IPD. Study Design Prospective clinical study, quality of life (QoL). Methods One-hundred mentally competent IPD patients with voice and swallowing complaints were asked to answer four QoL questionnaires (Voice Handicap Index, MD Anderson Dysphagia Inventory, Visual Analog Scale [VAS] voice, and Dysphagia Severity Scale [DSS]). Differences in means for the QoL questionnaires and their subscales within Hoehn and Yahr stage groups were calculated using one-way analysis of variance. The relationship between voice- and swallow-related QoL questionnaires was determined with the Spearman correlation coefficient. Results Scores on both voice and swallow questionnaires suggest an overall decrease in QoL with progression of IPD. A plateau in QoL for VAS voice and the DSS was seen in the early Hoehn and Yahr stages. Finally, scores on voice-related QoL questionnaires were significantly correlated with swallow-related QoL outcomes. Conclusions Voice- and swallow-related QoL decreases with progression of IPD. A significant association was found between voice- and swallow-related QoL questionnaires. Healthcare professionals can benefit from voice- and swallow-related QoL questionnaires in a multidimensional voice- or swallow-assessment protocol. The patient's perception of his/her voice and swallowing disorders and its impact on QoL in IPD should not be disregarded. Level of Evidence 2b Laryngoscope, 2015
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- 2015
8. The clinical and microbiological characteristics of enteric fever in Cambodia, 2008-2015
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Nizar Fawal, Jan Jacobs, Laura Maria Francisca Kuijpers, François-Xavier Weill, Chhun Heng Veng, Simon Le Hello, Laetitia Fabre, Willy Peetermans, Thong Phe, Erika Vlieghe, Sovann Ieng, Kruy Lim, Chun Kham, Institute of Tropical Medicine [Antwerp] (ITM), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Sihanouk Hospital Center of HOPE [Phnom Penh], Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS), Institut Pasteur [Paris] (IP), Antwerp University Hospital [Edegem] (UZA), University Hospitals Leuven [Leuven], The surveillance of bloodstream infections at the Sihanouk Hospital Center of HOPE is supported by the Belgian Directorate of Development Cooperation (DGD) (https://diplomatie.belgium.be/en/policy/development_cooperation/who_we_are/our_organisation/dgd) through project 2.08 of the Third Framework Agreement between the Belgian DGD (Ministry of Development Cooperation) and the Institute of Tropical Medicine (ITM). This study was further supported by the Strategic Network Laboratory Quality Management (LQM) project (http://www.labquality.be/) (Belgian Development Cooperation), the Institut Pasteur and the French government’s Investissement d’Avenir programme, Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (http://www.agence-nationale-recherche.fr/?ProjetIA=10-LABX-0062) (grant number ANR-10-LABX-62-IBEID). LMFK is supported by the Flemish Ministry of Sciences (EWI, SOFI project IDIS) (http://www.ewi-vlaanderen.be/en) and received additional travel grants (grant numbers K2.060.16N and K2.065.17N) from the Fund for Scientific Research Flanders (F.W.O.-Vlaanderen, Belgium) (http://www.fwo.be/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris]
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Bacterial Diseases ,0301 basic medicine ,Male ,Salmonella ,Salmonellosis ,Physiology ,Fevers ,Pathology and Laboratory Medicine ,Salmonella typhi ,medicine.disease_cause ,Salmonella Typhi ,Geographical Locations ,Medicine and Health Sciences ,Blood culture ,medicine.diagnostic_test ,biology ,lcsh:Public aspects of medicine ,Paratyphoid fever ,Salmonella paratyphi A ,Bacterial Pathogens ,Body Fluids ,3. Good health ,Anti-Bacterial Agents ,Ciprofloxacin ,Salmonella Enterica ,Blood ,Infectious Diseases ,Medical Microbiology ,Salmonella enterica ,Female ,Pathogens ,Anatomy ,Cambodia ,Research Article ,medicine.drug ,Asia ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,030106 microbiology ,Microbiology ,complex mixtures ,Typhoid fever ,03 medical and health sciences ,Signs and Symptoms ,Enterobacteriaceae ,Diagnostic Medicine ,Microbial Control ,Drug Resistance, Bacterial ,Paratyphoid Fever ,medicine ,Humans ,Typhoid Fever ,Microbial Pathogens ,Retrospective Studies ,Pharmacology ,Bacteria ,business.industry ,Organisms ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,lcsh:RA1-1270 ,biology.organism_classification ,medicine.disease ,Virology ,Antibiotic Resistance ,People and Places ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Human medicine ,Antimicrobial Resistance ,business - Abstract
Background Enteric fever remains a major public health problem in low resource settings and antibiotic resistance is increasing. In Asia, an increasing proportion of infections is caused by Salmonella enterica serovar Paratyphi A, which for a long time was assumed to cause a milder clinical syndrome compared to Salmonella enterica serovar Typhi. Methodology A retrospective chart review study was conducted of 254 unique cases of blood culture confirmed enteric fever who presented at a referral adult hospital in Phnom Penh, Cambodia between 2008 and 2015. Demographic, clinical and laboratory data were collected from clinical charts and antibiotic susceptibility testing was performed. Whole genome sequence analysis was performed on a subset of 121 isolates. Results One-hundred-and-ninety unique patients were diagnosed with Salmonella Paratyphi A and 64 with Salmonella Typhi. In the period 2008–2012, Salmonella Paratyphi A comprised 25.5% of 47 enteric fever cases compared to 86.0% of 207 cases during 2013–2015. Presenting symptoms were identical for both serovars but higher median leukocyte counts (6.8 x 109/L vs. 6.3 x 109/L; p = 0.035) and C-reactive protein (CRP) values (47.0 mg/L vs. 36 mg/L; p = 0.034) were observed for Salmonella Typhi infections. All but one of the Salmonella Typhi isolates belonged to haplotype H58 associated with multidrug resistance (MDR) (i.e. resistance to ampicillin, chloramphenicol and co-trimoxazole).;42.9% actually displayed MDR compared to none of the Salmonella Paratyphi A isolates. Decreased ciprofloxacin susceptibility (DCS) was observed in 96.9% (62/64) of Salmonella Typhi isolates versus 11.5% (21/183) of Salmonella Paratyphi A isolates (all but one from 2015). All isolates were susceptible to azithromycin and ceftriaxone. Conclusions In Phnom Penh, Cambodia, Salmonella Paratyphi A now causes the majority of enteric fever cases and decreased susceptibility against ciprofloxacin is increasing. Overall, Salmonella Typhi was significantly more associated with MDR and DCS compared to Salmonella Paratyphi A., Author summary Enteric fever is a bloodstream infection caused by the bacteria Salmonella Typhi or Salmonella Paratyphi A, B, or C. It is common in low resource settings and linked to poor water quality and sanitation. The disease is also endemic in Cambodia and since 2013 there has been a sharp increase in the number of Salmonella Paratyphi A infections. We sought to compare the clinical phenotypes and antibiotic susceptibilities of Salmonella Paratyphi A infections with those of Salmonella Typhi infections in this setting. We retrospectively collected demographic, clinical and laboratory data from clinical charts of 254 patients with a blood culture positive for enteric fever. We also assessed antibiotic susceptibility patterns and sequenced the genome of a subset of isolates. We found that since 2013 the majority of enteric fever cases are caused by Salmonella Paratyphi A which increasingly shows decreased susceptibility to the antibiotic ciprofloxacin, the current first line treatment. In contrast, in Salmonella Typhi a re-emergence of susceptibility for the former first line antibiotics of ampicillin, co-trimoxazole and chloramphenicol was observed. Presenting symptoms of Salmonella Typhi and Salmonella Paratyphi A were identical, minor differences were observed in laboratory parameters.
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- 2017
9. Ongoing outbreak of Salmonella enterica serovar Paratyphi A infections, Phnom Penh, Cambodia
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Panha Chung, Erika Vlieghe, Jan Jacobs, Dara Sar, Chhun Heng Veng, Thong Phe, Laura Maria Francisca Kuijpers, and Chun Kham
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Adult ,Male ,Adolescent ,Salmonella enterica serovar Paratyphi A ,Microbiology ,Disease Outbreaks ,Young Adult ,Virology ,Paratyphoid Fever ,Humans ,Medicine ,Aged ,Aged, 80 and over ,business.industry ,Incidence ,Paratyphoid fever ,Salmonella paratyphi A ,Outbreak ,General Medicine ,Middle Aged ,medicine.disease ,Phnom penh ,Infectious Diseases ,Female ,Parasitology ,Cambodia ,business - Abstract
This item has no abstract. Follow the links below to access the full text.
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- 2015
10. Genomic analysis of
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Laura Maria Francisca, Kuijpers, Simon, Le Hello, Nizar, Fawal, Laetitia, Fabre, Mathieu, Tourdjman, Muriel, Dufour, Dara, Sar, Chun, Kham, Thong, Phe, Erika, Vlieghe, Christiane, Bouchier, Jan, Jacobs, and François-Xavier, Weill
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whole genome sequencing ,Asia ,Communicable Disease Genomics ,Genomics ,Serogroup ,Disease Outbreaks ,Europe ,resistance ,Salmonella paratyphi A ,Microbial Evolution and Epidemiology ,Africa ,Drug Resistance, Bacterial ,Paratyphoid Fever ,Humans ,Cambodia ,Genome, Bacterial ,Research Paper - Abstract
In 2013, an unusual increase in the number of Salmonella enterica serotype Paratyphi A (Salmonella Paratyphi A) infections was reported in patients in Phnom Penh, Cambodia, and in European, American and Japanese travellers returning from Cambodia. Epidemiological investigations did not identify a common source of exposure. To analyse the population structure and genetic diversity of these Salmonella Paratyphi A isolates, we used whole-genome sequencing on 65 isolates collected from 1999 to 2014: 55 from infections acquired in Cambodia and 10 from infections acquired in other countries in Asia, Africa and Europe. Short-read sequences from 80 published genomes from around the world and from 13 published genomes associated with an outbreak in China were also included. Pulsed-field gel electrophoresis (PFGE) was performed on a subset of isolates. Genomic analyses were found to provide much more accurate information for tracking the individual strains than PFGE. All but 2 of the 36 isolates acquired in Cambodia during 2013–2014 belonged to the same clade, C5, of lineage C. This clade has been isolated in Cambodia since at least 1999. The Chinese outbreak isolates belonged to a different clade (C4) and were resistant to nalidixic acid, whereas the Cambodian outbreak isolates displayed pan-susceptibility to antibiotics. Since 2014, the total number of cases has decreased, but there has been an increase in the frequency with which nalidixic acid-resistant C5 isolates are isolated. The frequency of these isolates should be monitored over time, because they display decreased susceptibility to ciprofloxacin, the first-choice antibiotic for treating paratyphoid fever.
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- 2016
11. Severe anaemia associated with Plasmodium falciparum infection in children: consequences for additional blood sampling for research
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Issa Guiraud, Jessica Maltha, Palpouguini Lompo, Laura Maria Francisca Kuijpers, Hugo Devlieger, Jan Jacobs, Chris Van Geet, Berenger Kaboré, and Halidou Tinto
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Male ,Practice guidelines as topic ,medicine.medical_specialty ,Pediatrics ,Biomedical Research ,Anemia ,030231 tropical medicine ,Blood volume ,Specimen Handling ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Burkina Faso ,parasitic diseases ,Humans ,Medicine ,030212 general & internal medicine ,Malaria, Falciparum ,Child ,Rapid diagnostic test ,Diagnostic Tests, Routine ,business.industry ,Research ,Blood specimen collection ,Infant ,Guideline ,medicine.disease ,Surgery ,Infectious Diseases ,Child, Preschool ,Cohort ,Tropical medicine ,Female ,Parasitology ,business ,Blood sampling ,Cohort study - Abstract
BACKGROUND: Plasmodium falciparum infection may cause severe anaemia, particularly in children. When planning a diagnostic study on children suspected of severe malaria in sub-Saharan Africa, it was questioned how much blood could be safely sampled; intended blood volumes (blood cultures and EDTA blood) were 6 mL (children aged 2 months to
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- 2016
12. Invasive Salmonella Infections at Multiple Surveillance Sites in the Democratic Republic of the Congo, 2011-2014
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Laura Maria Francisca Kuijpers, Octavie Lunguya, Wesley Mattheus, Jan Verhaegen, Lisette Mbuyi Kalonji, Dadi Falay, Chris Van Geet, Jan Jacobs, Annelies Post, Jean-Jacques Muyembe, Marie-France Phoba, Barbara Barbé, Dauly Ngbonda, Pieter-Jan Ceyssens, and Sophie Bertrand
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Microbiology (medical) ,nontyphoidal Salmonella ,Adult ,Male ,Salmonella typhimurium ,Salmonella ,antibiotic resistance ,Adolescent ,Salmonella enteritidis ,Salmonella infection ,bloodstream infection ,Bacteremia ,Microbial Sensitivity Tests ,Azithromycin ,Salmonella typhi ,medicine.disease_cause ,Salmonella Typhi ,beta-Lactamases ,Microbiology ,Young Adult ,Antibiotic resistance ,Ciprofloxacin ,Drug Resistance, Multiple, Bacterial ,medicine ,Humans ,Blood culture ,Child ,Aged ,medicine.diagnostic_test ,business.industry ,Infant ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,Anti-Bacterial Agents ,Multiple drug resistance ,Infectious Diseases ,Child, Preschool ,Epidemiological Monitoring ,Salmonella Infections ,Democratic Republic of the Congo ,Female ,Seasons ,business ,medicine.drug - Abstract
BACKGROUND This study reports the microbiological landscape of Salmonella Typhi and invasive nontyphoidal Salmonella (iNTS) in the Democratic Republic of the Congo (DRC). METHODS Blood cultures obtained from hospital-admitted patients suspected of bloodstream infection (BSI) in 4 of 11 provinces in DRC (Kinshasa, Bas-Congo, Equateur, and Orientale) were processed. Sampling had started in 2007; the results for the period 2011-2014 are reported. RESULTS Salmonella Typhi and iNTS were cultured from 194 (1.4%) and 840 (5.9%), respectively, of 14,110 BSI episodes and ranked first among BSI pathogens in adults (65/300 [21.7%]) and children (783/1901 [41.2%]), respectively. A total of 948 of 1034 (91.7%) isolates were available for analysis (164 Salmonella Typhi and 784 iNTS). Salmonella Typhimurium and Salmonella Enteritidis represented 386 (49.2%) and 391 (49.9%), respectively, of iNTS isolates, fluctuating over time and geography and increasing during the rainy season. Adults accounted for
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- 2015
13. Microbiological, clinical and molecular findings of non-typhoidal Salmonella bloodstream infections associated with malaria, Oriental Province, Democratic Republic of the Congo
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Wesley Mattheus, Raymond Vanhoof, Emmanuel Vakaniaki, Hilde De Boeck, Octavie Lunguya, Pieter-Jan Ceyssens, Sophie Bertrand, Jan Jacobs, Laura Maria Francisca Kuijpers, Erik Verheyen, Dauly Ngbonda, Hugo Devlieger, Dadi Falay, Chris Van Geet, and Marie-France Phoba
- Subjects
Male ,Salmonella typhimurium ,Salmonella ,Bacteremia ,Azithromycin ,Molecular typing ,medicine.disease_cause ,Disease Outbreaks ,0302 clinical medicine ,Drug Resistance, Multiple, Bacterial ,Case fatality rate ,Blood culture ,030212 general & internal medicine ,Malaria, Falciparum ,Child ,Children ,medicine.diagnostic_test ,Coinfection ,Ceftriaxone ,Anti-Bacterial Agents ,Hospitalization ,Infectious Diseases ,Tandem Repeat Sequences ,Child, Preschool ,Salmonella Infections ,Democratic Republic of the Congo ,Female ,Research Article ,medicine.drug ,Adult ,Adolescent ,Salmonella enteritidis ,030231 tropical medicine ,Serogroup ,03 medical and health sciences ,Asian People ,parasitic diseases ,medicine ,Humans ,business.industry ,Infant, Newborn ,Antibiotic ,Infant ,Outbreak ,medicine.disease ,Virology ,Malaria ,Symptoms ,Human medicine ,Bloodstream infections ,business - Abstract
Background In sub-Saharan Africa, non-typhoidal Salmonella (NTS) can cause bloodstream infections, referred to as invasive non-typhoidal Salmonella disease (iNTS disease); it can occur in outbreaks and is often preceded by malaria. Data from Central Africa is limited. Methods Clinical, microbiological and molecular findings of NTS recovered in a blood culture surveillance project (2009–2014) were analyzed. Results In March-July 2012 there was an epidemic increase in malaria infections in the Oriental Province of the Democratic Republic of the Congo (DRC). In one referral hospital, overall hospital admissions in June 2012 were 2.6 times higher as compared to the same period in the years before and after (336 versus an average of 128 respectively); numbers of malaria cases and blood transfusions were nearly three- and five-fold higher respectively (317 versus 112 and 250 versus 55). Case fatality rates (in-hospital deaths versus all admissions) peaked at 14.6 %. Salmonella Typhimurium and Salmonella Enteritidis together accounted for 88.9 % of pathogens isolated from blood cultures collected during an outreach visit to the affected districts in June 2012. Children infected with Salmonella Enteritidis (33 patient files available) tended to be co-infected with Plasmodium falciparum more often than children infected with Salmonella Typhimurium (40 patients files available) (81.8 % versus 62.5 %). Through the microbiological surveillance project (May 2009–May 2014) 113 unique NTS isolates were collected (28.5 % (113/396) of pathogens); most (95.3 %) were recovered from children
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