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The dependency of the results from a network adjustment on the application used is investigated. For that purpose, the results obtained by each tested application on several sets of simulated measurements are compared. In each simulation, only one parameter varies. We first present our comparison methodology and the method that was used to add Gaussian-like errors to theoretical measurements. We then apply it to study the impact of the side length of the network and of the ellipsoidal height difference among points in the network for several network adjustment applications: Columbus, CoMeT, Geolab, JAG3D, LGC, Move3, Star*Net and Trinet+.

International audience; The estimation of tropospheric gradients in GNSS data processing is a well-known technique to improve positioning precision.To study the correlation between the tropospheric gradients and the topography, we computed Zenith Wet Delay (ZWD) and tropospheric gradients using the GIPSY-OASIS II SOFTWARE from 2 years of GPS observations recorded at 52 worldwide permanent stations, focusing on regions with significant relief. We observe that gradient directions are stable over time and point towards the relief for most of the considered stations. Based on these results, we discuss the physical meaning of the tropospheric horizontal gradients and we investigate why gradients have this particular direction for stations located nearby high mountains. The GPS stations were selected and classified into four main categories: stations close to a mountain range or an isolated mountain (class 1 and 2), stations surrounded by isolated mountains in several directions or in all directions (class 3 and 4). The correlation between the gradient direction and their magnitude with respect to mountain slopes was analysed. A very clear correlation appears for stations of classes 1 and 2 whereas no correlation is obvious for stations of classes 3 and 4. For 89% of stations in classes 1 and 2, a relevant correlation appears, varying between 0.4 and 1. For 64% of stations in classes 1 and 2, a relevant correlation appears, varying between 0.6 and 1. Horizontal gradients estimation show very significant amplitude and a stable direction all along the year, this main direction is most of the time pointing towards the direction of mountains. This behaviour can be explained by a vertical shift of the tropospheric layer due to the presence of mountains, close to the station and up to the maximum distance of 60 km from the station. This orientation does not seem to depend on seasons because no annual or bimonthly means variations appear for all stations. Moreover diurnal variations do not appear on the spatial distribution of the gradients and results are similar for neighbouring stations, separated by few km, which show that local effects such as multipath propagation have influence.

Chronic inflammation is now a well-known precursor for cancer development. Infectious prostatitis are the most common causes of prostate inflammation, but emerging evidence points the role of metabolic disorders as a potential source of cancer-related inflammation. Although the widely used treatment for prostate cancer based on androgen deprivation therapy (ADT) effectively decreases tumor size, it also causes profound alterations in immune tumor microenvironment within the prostate. Here, we demonstrate that prostates of a mouse model invalidated for nuclear receptors liver X receptors (LXRs), crucial lipid metabolism and inflammation integrators, respond in an unexpected way to androgen deprivation. Indeed, we observed profound alterations in immune cells composition, which was associated with chronic inflammation of the prostate. This was explained by the recruitment of phagocytosis-deficient macrophages leading to aberrant hyporesponse to castration. This phenotypic alteration was sufficient to allow prostatic neoplasia. Altogether, these data suggest that ADT and inflammation resulting from metabolic alterations interact to promote aberrant proliferation of epithelial prostate cells and development of neoplasia. This raises the question of the benefit of ADT for patients with metabolic disorders.

One of the most important but less understood step of epithelial tumourigenesis occurs when cells acquire the ability to leave their epithelial compartment. This phenomenon, described as basal epithelial cell extrusion (basal extrusion), represents the first step of tumour invasion. However, due to lack of adequate in vivo model, implication of emblematic signalling pathways such as Ras/Mitogen-Activated Protein Kinase (MAPK) and phosphoinositide 3 kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signalling pathways, is scarcely described in this phenomenon. We have developed a unique model of basal extrusion in the Drosophila accessory gland. There, we demonstrate that both Ras/MAPK and PI3K/AKT/mTOR pathways are necessary for basal extrusion. Furthermore, as in prostate cancer, we show that these pathways are co-activated. This occurs through set up of Epidermal Growth Factor Receptor (EGFR) and Insulin Receptor (InR) dependent autocrine loops, a phenomenon that, considering human data, could be relevant for prostate cancer., The molecular mechanisms leading to basal extrusion are unclear. Here, the authors use the Drosophila accessory gland to model human prostate acini and show that Ras/MAPK and PI3K/AKT/mTOR pathways are co-activated in two autocrine loops by dEGF and dIGF, inducing basal extrusion and subsequent tumour formation.

International audience; Magnetic cores are typically used in every stage of an electrical energy production and distribution chain. Local defects, including edge burrs and interlaminar faults, are detrimental for system performance and should be detected swiftly to ensure high reliability and durability. Real-time magnetic core condition monitoring is a promising solution for rapid fault detection. However, similar to dimensioning or performance evaluation, this monitoring has always been performed through averaged magnetic properties, which limits efficiency. In this domain, significant progress is forecasted by achieving precise local measurements. In this study, an innovative solution is proposed to measure local magnetic properties, which is adapted to real-time monitoring and magnetic circuit evaluation. The proposed sensor is a one-piece device that is flat enough to be placed noninvasively between the laminations at distinct positions in the magnetic core. It measures the magnetic excitation and induction fields in two dimensions, which can be used as local inputs for realtime condition monitoring. In this manuscript, the proposed sensor was first detailed, experimental results were provided, and finite-element simulations were performed. The sensor capability was validated both experimentally and through simulation. The results of this study contribute to the development of an intelligent magnetic core that includes an effective real-time monitoring system. The study provides a local validation of the most advanced highfidelity simulation method, which could be used to predict the responses of magnetic cores and electromagnetic converters to defects of various natures, as well as geometrical and property changes.

Leptospirosis is a zoonosis caused by the spirochete Leptospira. Most cases of leptospirosis are mild to moderate and self-limited. The course of disease, however, may be complicated by multiorgan dysfunction with liver and kidney failure causing Weil's disease. Leptospirosis is also rare among HIV-infected patients. We report a case of an HIV-infected patient with Weil's disease.

Today, it has become very easy to manipulate digital images using image processing tools and software such as Adobe Photoshop ( https://www.adobe.com/products/photoshop.html ). Tampering with images by splicing is an operation that consists of cutting-and-pasting an area of an image into another host image. In this paper, we propose to detect and localize such manipulations by analyzing the correlation of the image noise across the three color channels RGB, which is an intrinsic feature of the digital photography acquisition process. More precisely, we propose to detect the border between the background (host image) and the spliced area. Using a sliding window, we detect the blocks that span across the two areas which are characterized by two different color noise correlations. To do this, we propose specific features that are able to highlight these blocks. After the feature extraction, we introduce a learning phase using a Random Forest Classifier. Experimental results, specifically on the Columbia database, show very good results in comparison to other current state of the art methods.

Multiple studies have reported the metabolic benefits of high-intensity exercise programs like CrossFit. If these high-intensity exercises are not done in a proper structured pattern, adverse outcomes like rhabdomyolysis can occur. Here we discuss a case of a patient who undertook one session of CrossFit exercise and developed exertional rhabdomyolysis. A 22-year-old Caucasian male presented to the emergency department with complaints of generalized body ache and passage of dark-colored urine. His symptoms began after two days of an exhaustive session of CrossFit exercise. Blood test in the emergency showed elevated creatine kinase (CK) of 132,540 units per liter (U/L), normal renal function (creatinine and blood urea nitrogen), and normal serum electrolytes. His clinical symptoms and lab findings were consistent with exertional rhabdomyolysis. He was treated with aggressive intravenous fluids and oral hydration therapy. He did not develop any complication and he was discharged on the sixth day. This case report demonstrates a possible preventable rhabdomyolysis that developed secondary to undue participation in CrossFit exercise.

Chronic inflammation is now a well-known precursor for cancer development. Infectious prostatitis are the most common causes of prostate inflammation, but emerging evidence points the role of metabolic disorders as a potential source of cancer-related inflammation. Although the widely used treatment for prostate cancer based on androgen deprivation therapy (ADT) effectively decreases tumor size, it also causes profound alterations in immune tumor microenvironment within the prostate. Here, we demonstrate that prostates of a mouse model invalidated for nuclear receptors liver X receptors (LXRs), crucial lipid metabolism and inflammation integrators, respond in an unexpected way to androgen deprivation. Indeed, we observed profound alterations in immune cells composition, which was associated with chronic inflammation of the prostate. This was explained by the recruitment of phagocytosis-deficient macrophages leading to aberrant hyporesponse to castration. This phenotypic alteration was sufficient to allow prostatic neoplasia. Altogether, these data suggest that ADT and inflammation resulting from metabolic alterations interact to promote aberrant proliferation of epithelial prostate cells and development of neoplasia. This raises the question of the benefit of ADT for patients with metabolic disorders., Mice lacking the liver X nuclear receptors (LXRs), crucial integrators of lipid metabolism, were used to study the response of the prostate to androgen deprivation. This reveals that lack of androgens leads to chronic inflammation due to impaired clearance of castration-induced apoptotic cells, allowing production of pro-inflammatory cytokines and promoting prostate neoplasia.

International audience; The magnetic needle probe method is a technique used to locally measure the magnetic state of a magnetic component. This concept has a rich conceptual history, but it has rarely been used in the industrial field. There are mainly two reasons for this: instrumentation limitations and inappropriate sizes of sensors’ geometries. The first limitation has been overcome recently due to large improvements in both the analog and digital electronic fields. In this study, the second limitation, size/geometry is addressed by printing the magnetic needle probe using conductive ink directly on the ferromagnetic element to be controlled. The resulting sensor exhibits a drastic volume reduction. Such improvements allow measurement of the magnetic state of a previously inaccessible magnetic lamination through a magnetic laminated core. This opens up the possibility of measuring in situ magnetic behavior and monitoring many electromagnetic devices such as electric transformers, AC/DC electric motors, or even real-time electromagnetic non-destructive testing of ferromagnetic steel components. Over the past few years, simulation approaches including space discretization methods: finite elements, finite differences, and boundary elements have been proposed to describe the internal behavior of magnetic lamination stacks but experimental results validating these simulations could not be realized due to the aforementioned limitations. The printed magnetic needle probe method (PMNPM) described in this paper can be used to collect such local information and to validate homogenization methods. Experimental validations are proposed by comparing the sum of the laminations magnetic induction, they are individually measured with the PMNPM method to calculate the average induction obtained from a surrounding coil.

A network adjustment program is an essential tool for surveyors. It allows the computation of point coordinates and confidence regions from measurements undertaken in the field. It also hel...

Made available in DSpace on 2020-12-12T01:15:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-15 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) PPP (Precise Point Positioning) is a GNSS (Global Navigation Satellite Systems) positioning method that requires SSR (State Space Representation) corrections in order to provide solutions with an accuracy of centimetric level. The so-called RT-PPP (Real-time PPP) is possible thanks to real-time precise SSR products, for orbits and clocks, provided by IGS (International GNSS Service) and its associate analysis centers such as CNES (Centre National d'Etudes Spatiales). CNES SSR products also enable RT-PPP with integer ambiguity resolution. In GNSS related literature, PPP with ambiguity resolution (PPP-AR) in real-time is often referred as PPP-RTK (PPP – Real Time Kinematic). PPP-WIZARD (PPP - With Integer and Zero-difference Ambiguity Resolution Demonstrator) is a software that is made available by CNES. This software is capable of performing PPP-RTK. It estimates slant ionospheric delays and other GNSS positioning parameters. Since ionospheric effects are spatially correlated by GNSS data from active networks, it is possible to model and provide ionospheric delays for any position in the network coverage area. The prior knowledge ionospheric delays can reduce positioning convergence for PPP-RTK users. Real-time ionospheric models could benefit from highly precise ionospheric delays estimated in PPP-AR. In this study, we demonstrate that ionospheric delays obtained throughout PPP-AR estimation are actu ally ionospheric observables. Ionospheric observables are biased by an order of few meters caused by the receiver hardware biases. These biases prohibit the use of PPP-WIZARD ionospheric delays to produce ionospheric models. Receiver biases correction is essential to provide ionospheric delays while using PPP-AR based ionospheric observables. In this contribution, a method was implemented to estimate and mitigate receiver hardware biases influence on slant ionospheric observables from PPP-AR. In order to assess the proposed approach, PPP-AR data from 12 GNSS stations were processed over a two-month period (March and April 2018). A comparison between IGS ionospheric products and PPP-AR based ionospheric observables corrected for receiver biases, resulted in a mean of differences of −39 cm and 51 cm standard deviation. The results are consistent with the accuracy of the IGS ionospheric products, 2–8 TECU, considering that 1 TECU is ~16 cm in L1. In another analysis, a comparison of ionospheric delays from 5 pairs of short baselines GNSS stations found an agreement of 0.001 m in mean differences with 22 cm standard deviation after receiver biases were corrected. Therefore, the proposed solution is promising and could produce high quality (1–2 TECU) slant ionospheric delays. This product can be used in a large variety of modeling approaches, since ionospheric delays after correction are unbiased. These results indicate that the proposed strategy is promising, and could benefit applications that require accuracy of 1–2 TECU (~16–32 cm in L1). PPGCG/UFPR PPGCC/Unesp G&F/CNAM PPGCC/Unesp CNPq: 465648/2014-2

The facile synthesis of functionalized luminescent nanoparticles from LnL3 lanthanide complexes is described. The luminescence properties of the lanthanide chelates and of the corresponding nanohybrids are reported and compared. For a further application in bioimaging, the cytotoxicity of the nano-objects was investigated.

International audience; An alternative sensing solution is described to measure local magnetic hysteresis cycles through a laminated magnetic core. Due to the reduced space gap separating two successive laminations, it is impossible to interpose the usual oversize magnetic sensors (wound coil, Halleffect sensor). In this study, the space issue has been solved by printing the needle probe method for the magnetic state monitoring and by using a micrometric Giant Magneto Resistance (GMR) for the magnetic excitation measurement. An instrumented magnetic lamination including the non-invasive monitoring solution has been built and moved successively to every lamination position of the whole laminated ferromagnetic core. A precise cartography of the hysteresis losses has been reconstructed from all these local measurements and the average values compared to the classic measurement methods obtained with a wound coil. The relative agreement between the experimental results observed opened doors to large improvement in the estimation of magnetic losses and in the design of magnetic circuits.

International audience; Increasing the energy efficiency is one of the most important issues in modern power electronic systems. In aircraft applications, the energy efficiency must be associated with a maximum reduction of mass and volume, so a high components compactness. A consequence from this compactness is the increase of operating temperature. Thus, the magnetic materials used in these applications, have to work at high temperature. It raises the question of the thermal ageing problem. The reliability of these components operating at this condition becomes a real problem which deserves serious interest. Our work takes part in this context by studying the magnetic material thermal ageing. The nanocrystalline materials are getting more and more used in power electronic applications. Main advantages of nanocrystalline materials compared to ferrite are: high saturation flux density of almost 1.25 T and low dynamic losses for low and medium frequencies. The nanocrystalline Fe73.5Cu1Nb3Si15.5B7 alloys have been chosen in our aging study. This study is based on monitoring the magnetic characteristics for several continuous thermal ageing (100, 150, 200 and 240 °C). An important experimental work of magnetic characterization is being done following a specific monitoring protocol. Elsewhere, X-Ray Diffraction and magnetostriction measurements were carried out to support the study of the anisotropy energies evolution with ageing. This latter is discussed in this paper to explain and give hypothesis about the ageing phenomena.

Karsts of Patagonia (Madre de Dios archipelago, Chile, 50° S) are among the more wettest in the world (> 8 000 mm. year-1). The rate of surface dissolution on bare karst is estimated at more than 100 mm/ millennium from glacial erratics, exhumed dykes or historical paintings. We have instrumented an experimental watershed on karren and recorded meteorological parameters (precipitation, temperature, wind speed, direction) and parameters of runoff into karren (discharge, conductivity, temperature) used to understand the flow on limestone in subpolar oceanic setting. The wind, spray and especially the rainfalls explain the dissolution of the limestone surface. A topographic survey shows the drainage stairs organization whose genesis and evolution seem to be conditioned by running «step pool system » resulting in high variability of flows during the flood event., Les karsts de Patagonie (archipel de Madre de Dios, Chili, 50° S) comptent parmi les plus arrosés de la planète (> 8 000 mm/ an). À partir de socles d’erratiques glaciaires, de dykes exhumés ou de peintures historiques, les taux de dissolution superficielle sur karst nu sont estimés supérieurs à 100 mm/ millénaire. Nous avons instrumenté un bassin-versant sur lapiés nu ; le suivi de paramètres météorologiques (précipitation, température, vitesse et direction du vent) et de paramètres de ruissellement dans les rigoles (débit, conductivité, température) permet d’affiner la connaissance de l’écoulement sur calcaire en contexte océanique subpolaire. Le vent, les embruns et surtout le régime des précipitations contrôlent les modalités de la dissolution des calcaires en surface. Un relevé topographique fin permet de montrer une organisation du drainage en marches d’escalier dont la genèse et l’évolution semblent conditionner un fonctionnement en « step-pool system » se traduisant notamment par une forte variabilité des débits durant l’onde de crue., Jaillet Stéphane, Morel Laurent, Maire Richard, Malet Emmanuel, Marbach Georges, Morales Denis, Chenu Florian, Hobléa Fabien, Tourte Bernard, Expéditions Ultima Patagonia. Ruissellement et dissolution d’un bassin versant lapiazé des karsts de Patagonie, île Tarlton, archipel de Madre de Dios (Chili). In: Collection EDYTEM. Cahiers de géographie, numéro 19, 2017. Monitoring en milieux naturels. Retours d’expériences en terrains difficiles. pp. 137-146.

Over the last decade, near real-time analysis of GPS data has become a well-established atmospheric observing tool, primarily coordinated by the EIG EUMETNET GPS Water Vapour Programme (E-GVAP) in Europe. In the near future, four operational GNSS will be available for commercial and scientific applications with atmospheric science benefiting from new signals from up to 60 satellites observed at any one place and time, however, many challenges remain regarding their optimal combined utilization. Besides raw data streaming, recent availability of precise real-time orbit and clock corrections enable wide utilization of autonomous Precise Point Positioning (PPP), which is particularly efficient for high-rate, real-time and multi-GNSS analyses.

International audience; Mechanical characterization of electrical steels in an attempt to predict component fatigue has drawn more and more attention to magneto-mechanical coupling in ferromagnetic materials. In this paper, experimental evidence on the magneto-mechanical coupling of a thin Fe-Ni50 sheet subject to multiaxial stress due to electromagnetic peening-induced residual stresses is presented. A comprehensive model of the stress-dependent magnetostriction is validated using the Jiles-Atherton-Sablik (JAS) model for scalar ferromagnetic hysteresis. The JAS parameters are determined from an optimization algorithm, and the dependence of the model accuracy regarding its own parametrization is analyzed. Good correlation of experimentation data with the JAS model was obtained upon careful optimization of the model parameters.

International audience; Liver X receptors (LXRs) α (NR1H3) and β (NR1H2) are nuclear receptors that have been involved in the regulation of many physiological processes, principally in the control of cholesterol homeostasis, as well as in the control of the cell death and proliferation balance. These receptors are thus promising therapeutic targets in various pathologies such as dyslipidemia, atherosclerosis, diabetes and/or cancers. These receptors are known to be activated by specific oxysterol compounds. The screening for LXR-specific ligands is a challenging process: indeed, these molecules should present a specificity towards each LXR-isoform. Because some natural products have significant effects in the regulation of the LXR-regulated homeostasis and are enriched in flavonoids, we have decided to test in cell culture the effects of 4 selected flavonoids (galangin, quercetin, apigenin and naringenin) on the modulation of LXR activity using double-hybrid experiments. In silico, molecular docking suggests specific binding pattern between agonistic and antagonistic molecules. Altogether, these results allow a better understanding of the ligand binding pocket of LXRα/β. They also improve our knowledge about flavonoid mechanism of action, allowing the selection and development of better LXR selective ligands.

International audience; BACKGROUND: Deregulation of cholesterol metabolism represents a hallmark of prostate cancer (PCa) and promotes its development. OBJECTIVE: To compare cholesterol metabolism on individual paired normal and tumour prostate tissues obtained from patients with PCa. DESIGN, SETTING, AND PARTICIPANTS: Between 2008 and 2012, normal and tumour paired tissue samples were collected from radical prostatectomy specimens from a cohort of 69 patients treated for localised PCa. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Tumour and normal tissues were subjected to gene analysis, sterol measurement, and immunohistochemistry. The Wilcoxon paired test and Spearman test were applied for comparison and correlation analyses, respectively. Principal component analysis was also carried out to investigate relationships between quantitative variables. RESULTS AND LIMITATIONS: Overall, cholesterol concentrations were not significantly different between tissue pairs. However, tumour samples were significantly associated with downregulated de novo cholesterol synthesis, but exhibited 54.7% overexpression of SCARB1 that could increase high-density lipoprotein uptake in PCa. Tumour tissues showed different trafficking of available cholesterol, with significantly lower ACAT1, and an altered efflux via APOE. Furthermore, cholesterol metabolism in tumour tissues was characterised by higher accumulation of 7alpha-hydroxycholesterol (OHC), 7betaOHC, and 7-ketosterol, and a lower level of 27OHC. CONCLUSIONS: Focusing on individually paired prostate tissues, our results highlighted several differences between normal and tumour samples linked to a metabolic shift in cholesterol flux. PCa samples exhibited a specific tissue signature characterised by higher SCARB1 expression, higher accumulation of OHC species, and clear downregulation of de novo cholesterol synthesis. PATIENT SUMMARY: Comparing normal and tumour tissues from the same prostates, our study identified a set of alterations in prostate cancer samples in terms of their use of cholesterol. These included higher cholesterol uptake, accumulation of oxidised cholesterol derivatives, and autonomous cellular production of cholesterol. Together, these data provide promising clinical targets to fight prostate cancer.

International audience; Signal Transducer and Activator of Transcription 3 (STAT3) is aberrantly activated in glioblastoma and has been identified as a relevant therapeutic target in this disease and many other human cancers. After two decades of intensive research, there is not yet any approved STAT3-based glioma therapy. In addition to the canonical activation by tyrosine 705 phosphorylation, concordant reports described a potential therapeutic relevance of other post-translational modifications including mainly serine 727 phosphorylation. Such reports reinforce the need to refine the strategy of targeting STAT3 in each concerned disease. This review focuses on the role of serine 727 and tyrosine 705 phosphorylation of STAT3 in glioma. It explores their contribution to glial cell transformation and to the mechanisms that make glioma escape to both immune control and standard treatment.

The mammalian target of rapamycin (mTOR) plays essential roles in the regulation of growth-related processes such as protein synthesis, cell sizing and metabolism in both normal and pathological growing conditions. These functions of mTOR are thought to be largely a consequence of its cytoplasmic activity in regulating translation rate, but accumulating data highlight supplementary role(s) for this serine/threonine kinase within the nucleus. Indeed, the nuclear activities of mTOR are currently associated with the control of protein biosynthetic capacity through its ability to regulate the expression of gene products involved in the control of ribosomal biogenesis and proliferation. Using primary murine embryo fibroblasts (MEFs), we observed that cells with overactive mTOR signaling displayed higher abundance for the growth-associated Npm1 protein, in what represents a novel mechanism of Npm1 gene regulation. We show that Npm1 gene expression is dependent on mTOR as demonstrated by treatment of wild-type and Pten inactivated MEFs cultured with rapamycin or by transient transfections of small interfering RNA directed against mTOR. In accordance, the mTOR kinase localizes to the Npm1 promoter gene in vivo and it enhances the activity of a human NPM1-luciferase reporter gene providing an opportunity for direct control. Interestingly, rapamycin did not dislodge mTOR from the Npm1 promoter but rather strongly destabilized the Npm1 transcript by increasing its turnover. Using a prostate-specific Pten-deleted mouse model of cancer, Npm1 mRNA levels were found up-regulated and sensitive to rapamycin. Finally, we also showed that Npm1 is required to promote mTOR-dependent cell proliferation. We therefore proposed a model whereby mTOR is closely involved in the transcriptional and posttranscriptional regulation of Npm1 gene expression with implications in development and diseases including cancer.

An extensive validation of line-of-sight tropospheric slant total delays (STD) from Global Navigation Satellite Systems (GNSS), ray tracing in numerical weather prediction model (NWM) fields and microwave water vapour radiometer (WVR) is presented. Ten GNSS reference stations, including collocated sites, and almost 2 months of data from 2013, including severe weather events were used for comparison. Seven institutions delivered their STDs based on GNSS observations processed using 5 software programs and 11 strategies enabling to compare rather different solutions and to assess the impact of several aspects of the processing strategy. STDs from NWM ray tracing came from three institutions using three different NWMs and ray-tracing software. Inter-techniques evaluations demonstrated a good mutual agreement of various GNSS STD solutions compared to NWM and WVR STDs. The mean bias among GNSS solutions not considering post-fit residuals in STDs was -0.6 mm for STDs scaled in the zenith direction and the mean standard deviation was 3.7 mm. Standard deviations of comparisons between GNSS and NWM ray-tracing solutions were typically 10 mm +/- 2 mm (scaled in the zenith direction), depending on the NWM model and the GNSS station. Comparing GNSS versus WVR STDs reached standard deviations of 12 mm +/- 2 mm also scaled in the zenith direction. Impacts of raw GNSS post-fit residuals and cleaned residuals on optimal reconstructing of GNSS STDs were evaluated at intertechnique comparison and for GNSS at collocated sites. The use of raw post-fit residuals is not generally recommended as they might contain strong systematic effects, as demonstrated in the case of station LDB0. Simplified STDs reconstructed only from estimated GNSS tropospheric parameters, i.e. without applying post-fit residuals, performed the best in all the comparisons; however, it obviously missed part of tropospheric signals due to non-linear temporal and spatial variations in the troposphere. Although the post-fit residuals cleaned of visible systematic errors generally showed a slightly worse performance, they contained significant tropospheric signal on top of the simplified model. They are thus recommended for the reconstruction of STDs, particularly during high variability in the troposphere. Cleaned residuals also showed a stable performance during ordinary days while containing promising information about the troposphere at low-elevation angles. Web of Science 10 6 2208 2183

Les mesures issues de l'interférométrie d'images radar (InSAR), notamment satellitales, doivent être corrigées du délai dû à la propagation des ondes dans l'atmosphère. Plusieurs travaux ont déjà montré l'intérêt de cette correction. Cet article présente une méthode d'estimation de la phase troposphérique qui utilise à la fois des mesures GNSS (Global Navigation Satellite System) et un modèle d'atmosphère global (ERA-Interim). Pour évaluer cette méthode, nous comparons alors les mesures de déplacements interférométriques avant et après la correction avec les déplacements mesurés par GNSS, considérés comme référence. Les données utilisées pour les expériences sont acquises sur le Piton de la Fournaise en France.

Radiotherapy is an essential component of glioma standard treatment. Glioblastomas (GBM), however, display an important radioresistance leading to tumor recurrence. To improve patient prognosis, there is a need to radiosensitize GBM cells and to circumvent the mechanisms of resistance caused by interactions between tumor cells and their microenvironment. STAT3 has been identified as a therapeutic target in glioma because of its involvement in mechanisms sustaining tumor escape to both standard treatment and immune control. Here, we studied the role of STAT3 activation on tyrosine 705 (Y705) and serine 727 (S727) in glioma radioresistance. This study explored STAT3 phosphorylation on Y705 (pSTAT3-Y705) and S727 (pSTAT3-S727) in glioma cell lines and in clinical samples. Radiosensitizing effect of STAT3 activation down-modulation by Go6976 was explored. In a panel of 15 human glioma cell lines, we found that the level of pSTAT3-S727 was correlated to intrinsic radioresistance. Moreover, treating GBM cells with Go6976 resulted in a highly significant radiosensitization associated to a concomitant pSTAT3-S727 down-modulation only in GBM cell lines that exhibited no or weak pSTAT3-Y705. We report the constitutive activation of STAT3-S727 in all GBM clinical samples. Targeting pSTAT3-S727 mainly in pSTAT3-Y705-negative GBM could be a relevant approach to improve radiation therapy.

International audience; The Pyrenean mountain range is a slowly deforming belt with continuous and moderate seismic activity. To quantify its deformation field, we present the velocity field estimated from a GPS survey of the Pyrenees spanning 18 yr. The PotSis and ResPyr networks, including a total of 85 GPS sites, were installed and first measured in 1992 and 1995–1997, respectively, and remeasured in 2008 and 2010. We obtain a deformation field with velocities less than 1 mm yr−1 across the range. The estimated velocities for individual stations do not differ significantly from zero with 95 per cent confidence. Even so, we estimate a maximum extensional horizontal strain rate of 2.0 ± 1.7 nanostrain per year in a N–S direction in the western part of the range. We do not interpret the vertical displacements due to their large uncertainties. In order to compare the horizontal strain rates with the seismic activity, we analyse a set of 194 focal mechanisms using three methods: (i) the ‘r’ factor relating their P and T axes, (ii) the stress tensors obtained by fault slip inversion and (iii) the strain-rate tensors. Stress and strain-rate tensors are estimated for: (i) the whole data set, (ii) the eastern and western parts of the range separately, and (iii) eight zones, which are defined based on the seismicity and the tectonic patterns of the Pyrenees. Each of these analyses reveals a lateral variation of the deformation style from compression and extension in the east to extension and strike-slip in the west of the range. Although the horizontal components of the strain-rate tensors estimated from the seismic data are slightly smaller in magnitude than those computed from the GPS velocity field, they are consistent within the 2σ uncertainties. Furthermore, the orientations of their principal axes agree with the mapped active faults.

International audience; Estimation of tropospheric gradients in GNSS data processing is a well-known technique to improve positioning (e.g. Bar-Sever et al., 1998; Chen and Herring, 1997). More recently, several authors also focused on the estimation of such parameters for meteorological studies and demonstrated their potential benefits (e.g. Champollion et al., 2004). Today, they are routinely estimated by several global and regional GNSS analysis centres but they are still not yet used for operational meteorology.This paper discusses the physical meaning of tropospheric gradients estimated from GPS observations recorded in 2011 by 13 permanent stations located in Corsica Island (a French Island in the western part of Italy). Corsica Island is a particularly interesting location for such study as it presents a significant environmental contrast between the continent and the sea, as well as a steep topography.Therefore, we estimated Zenith Total Delay (ZTD) and tropospheric gradients using two software: GAMIT/GLOBK (GAMIT version 10.5) and GIPSY-OASIS II version 6.1. Our results are then compared to radiosonde observations and to the IGS final troposphere products. For all stations we found a good agreement between the ZWD estimated by the two software (the mean of the ZWD differences is 1 mm with a standard deviation of 6 mm) but the tropospheric gradients are in less good agreement (the mean of the gradient differences is 0.1 mm with a standard deviation of 0.7 mm), despite the differences in the processing strategy (double-differences for GAMIT/GLOBK versus zero-difference for GIPSY-OASIS).We also observe that gradient amplitudes are correlated with the seasonal behaviour of the humidity. Like ZWD estimates, they are larger in summer than in winter. Their directions are stable over the time but not correlated with the IWV anomaly observed by ERA-Interim. Tropospheric gradients observed at many sites always point to inland throughout the year. These preferred directions are almost opposite to the largest slope of the local topography as derived from the world Digital Elevation Model ASTER GDEM v2. These first results give a physical meaning to gradients but the origin of such directions need further investigations.

International audience; It is found to be one of the major issues while designing an aircraft, mass and volume have to be reduced in order to achieve energy efficiency. This leads to a high compactness of the electrical components which enables them to withstand at high temperatures. The magnetic components which are responsible for the electrical energy conversion, therefore exposed to high temperatures in working conditions. Their thermal ageing becomes a serious problem and deserves a particular attention. The FeCuNbSiB nanocrystalline materials have been selected for this ageing study because they are used in power electronic systems very frequently. The objective of the study is based on monitoring the magnetic characteristics under the condition of several continuous thermal ageing (100, 150, 200 and 240 °C). An important, experimental work of magnetic characterization is being done through a specific monitoring protocol and X-ray diffraction (XRD) along with magnetostriction measurements was carried out to support the study of the evolution of the anisotropy energies with aging. The latter is discussed in this paper to explain and give the hypothesis about the aging phenomena.

International audience; Since their birth or mergence the late 1980s, the nanocrystalline ultrasoft magnetic materials are taking a great importance in power electronic systems conception. One of the main advantages that make them more attractive nowadays is their ability to be packaged since the reduction of the magnetostrictive constant to almost zero. In aircraft applications, due to the high component compactness and to their location (for example near the jet engine), the operating temperature increases and may reach easily 200 °C and more. Consequently, the magnetic thermal ageing may occur but is, unfortunately, weakly studied. This paper focuses on the influence of the coating (packaging type) on the magnetic nanocrystalline performances during a thermal ageing. This study is based on monitoring the magnetic characteristics of two types of nanocrystalline cores (naked and coated) during a thermal activated ageing (100, 150 and 200 °C). Based on a dedicated monitoring protocol, a large magnetic characterization has been done and analyzed. Elsewhere, X-Ray Diffraction and magnetostriction measurements were carried out to support the study of the anisotropy energies evolution with ageing. This latter is discussed in this paper to explain and give hypothesis about the ageing phenomena.

The environmental issues are a strategic stake for the management of the planet. Our project falls under this context, at the interface man/ environment, by the means of the underground karstic field which is regarded as a recorder of environment evolution because located safe from erosion. Caves conceal detrital rhytmic deposits, still little studied, but very interesting from an environmental point of view., Dans le cadre des changements environnementaux, les études sur les milieux naturels sont des enjeux stratégiques pour la recherche scientifique. Cette recherche s’appuie sur une instrumentation autonome et adaptée à des problématiques spécifiques. Elle est orientée sur la mesure de phénomènes non-continus, fortement non-linéaires, lié à des effets de seuils et de stockage. Cette étude s’inscrit dans ce contexte, en domaine karstique souterrain, considéré comme un enregistreur performant de l’évolution de l’environnement car situé à l’abri de l’érosion. Les accumulations sédimentaires (détritiques ou carbonatées), enregistrent ces évolutions et sont utilisées en paléoclimatologie par exemple., Morel Laurent, Jaillet Stéphane, Lignier Vincent, Maire Richard. Instrumentation et caractérisation pour l’étude des dépôts sédimentaires dans les grottes. In: Collection EDYTEM. Cahiers de géographie, numéro 19, 2017. Monitoring en milieux naturels. Retours d’expériences en terrains difficiles. pp. 243-248.

Made available in DSpace on 2018-12-11T16:40:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 Precise Point Positioning (PPP) is a well-known technique of positioning by Global Navigation Satellite Systems (GNSS) that provides accurate solutions. With the availability of real-time precise orbit and clock products provided by the International GNSS Service (IGS) and by individual analysis centers such as Centre National d’Etudes Spatiales through the IGS Real-Time Project, PPP in real time is achievable. With such orbit and clock products and using dual-frequency receivers, first-order ionospheric effects can be eliminated by the ionospheric-free combination. Concerning the tropospheric delays, the Zenith Hydrostatic Delays can be quite well modeled, although the Zenith Wet Delays (ZWDs) have to be estimated because they cannot be mitigated by, for instance, observable combinations. However, adding ZWD estimates in PPP processing increases the time to achieve accurate positions. In order to reduce this convergence time, we (1) model the behavior of troposphere over France using ZWD estimates at Orphéon GNSS reference network stations and (2) send the modeling parameters to the GNSS users to be introduced as a priori ZWDs, with an appropriate uncertainty. At the user level, float PPP-RTK is achieved; that is, GNSS data are performed in kinematic mode and ambiguities are kept float. The quality of the modeling is assessed by comparison with tropospheric products published by Institut National de l’Information Géographique et Forestière. Finally, the improvements in terms of required time to achieve 10-cm accuracy for the rover position (simulated float PPP-RTK) are quantified and discussed. Results for 68 % quantiles of absolute errors convergence show that gains for GPS-only positioning with ZWDs derived from the assessed tropospheric modeling are about: 1 % (East), 20 % (North), and 5 % (Up). Since ZWD estimation is correlated with satellite geometry, we also investigated the positioning when processing GPS + GLONASS data, which increases significantly the number of available satellites. The improvements achieved by adding tropospheric corrections in this case are about: 2 % (East), 5 % (North), and 13 % (Up). Finally, a reduction in the number of reference stations by using a sparser network configuration to perform the tropospheric modeling does not degrade the generated tropospheric corrections, and similar performances are achieved. GeF Cnam Geodata Diffusion PPGCC/Unesp PPGCC/Unesp

Luirographe is a recording and measuring apparatus of water heights in underground cavities. It makes it possible to better know and understand the hydrological operation of the cavities. The study must be achieved over several years. The flood frequency and the height of water, the rising times, the speed of the wave in the cavity, the rise and fall times of the level of water can be studied. We can also study if there are outfalls and know in which order and at which speed the galleries are flooded. For example, in Luire cave, the arrival of walls of water in certain galleries, the outfalls and the flow of rivers have been studied., Le Luirographe est un appareil de mesure et d’enregistrement des hauteurs d’eau dans les cavités souterraines. Il permet de mieux connaître et de comprendre le fonctionnement hydrologique des systèmes karstiques. Sur plusieurs années, on peut connaître la fréquence des crues, la hauteur d’ennoiement, les temps de réaction, la vitesse de l’onde de crues dans la cavité, les temps de montée et de descente du niveau de l’eau. On peut également déterminer l’existence de déversoirs ou connaître dans quel ordre et à quelle vitesse les galeries s’ennoient. Par exemple, dans la Luire, on a ainsi pu constater l’arrivée de « murs d’eau » dans certaines galeries, identifier des déversoirs et déterminer l’écoulement de rivières souterraines qui n’ont jamais été observées par les spéléologues., Morel Laurent. Le Luirographe, développement d’un enregistreur autonome motivé par les mises en charge de la grotte de la Luire (Vercors, France). In: Collection EDYTEM. Cahiers de géographie, numéro 19, 2017. Monitoring en milieux naturels. Retours d’expériences en terrains difficiles. pp. 161-167.

Located in the italian massif of Marguareis, Scarasson is a famous cave because of its underground glacier and the operation “ Beyond Time” of Michel Siffre in 1962. Because of the quick melting of the glacier since the 2000 years, due to stop of snow feeding, a monitoring began in 2008 for temperature and humidity, and for measuring the retreat with ultrasonic sensors. The results show variations in temperature at different scales, the great dynamic complexity of an «open » underground climate system, and a remarkable transformation of the ice around the melting point., Situé dans le massif du Marguareis (France-Italie), le gouffre de Scarasson est une cavité connue en raison de la présence d’un glacier souterrain et de l’expérience «hors du temps » menée par Michel Siffre en 1962. La fonte accélérée du glacier depuis les années 2000 en lien probable avec l’arrêt de l’alimentation neigeuse a mené à une étude instrumentée de la cavité. Débutée en 2008, elle a été conduite avec des capteurs de température, d’humidité et de distance à ultrasons. Les résultats montrent des variations de la température à différentes échelles, la complexité du système climatique souterrain «ouvert » et la transformation de la glace autour du point de fusion., Morel Laurent, Maire Richard, Valla François, Decker Joan, Siffre Michel, Boscart Joffrey, Caillault Serge, Lambolglia Cathy, Lamboglia Jo, Petit Jean-robert, Ogand Michel. Fonte du glacier souterrain de Scarasson (Marguareis, Italie). Suivi instrumenté de la température, de l’humidité et du recul. In: Collection EDYTEM. Cahiers de géographie, numéro 19, 2017. Monitoring en milieux naturels. Retours d’expériences en terrains difficiles. pp. 101-108.

Several attempts to combine GNSS and InSAR for deformation measurements have been published in the last years. In this work, we propose a global inverse method that combines phase measurements from GNSS and spaceborne radar interferometry to estimate the ground vertical displacement. It is based on the PS (permanent scatterer) and least squares approaches and considers non-ambiguous phases. The exploratory method is tested using simulated data.

Complexes of the type [PdX(PPh3)(1)]X [1 = 4-phenyl-3-thiosemicarbazide; X = Cl- (2), Br- (3), I- (4), and SCN- (5)] have been synthesized and characterized by elemental analyses and IR, UV/Vis, and 1H and 13C NMR spectroscopy. The molecular structure of complex 4 was determined by single-crystal X-ray diffraction. The binding of the complexes with a purine base (guanosine) was investigated by 1H NMR spectroscopy and mass spectrometry, which showed the complexes to coordinate to guanosine through N7. A gel electrophoresis assay demonstrated the ability of 2-5 to cleave DNA plasmid. All the complexes were tested in vitro by means of the MTT assay for their cytotoxicity against two murine cell lines, LM3 (mammary adenocarcinoma) and LP07 (lung adenocarcinoma), and compared with cisplatin. Complexes 2-5 exhibited good cytotoxicity that surpasses that of cisplatin in the case of LM3.