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4. AN OUTBREAK OF EXTENSIVELY DRUG-RESISTANT ACINETOBACTER BAUMANNII IN A BELGIAN TERTIARY BURN WOUND CENTER.

Author

Costescu Strachinaru, D. I., Gallez, J-L., Verroken, A., Wagemans, J., Lood, C., De Vos, D., Pirnay, J-P., Lavigne, R., Rose, T., Strachinaru, M., Vanbrabant, P., and Soentjens, P.

Subjects
BURN care units, WHOLE genome sequencing, NOSOCOMIAL infections, INTENSIVE care units, BODY surface area
Abstract

Copyright of Annals of Burns & Fire Disasters is the property of Euro-Mediterranean Council for Burns & Fire Disasters and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2024

7. DUAL APPLICATION OF BACTERIOPHAGES AND MEROPENEM USING MICROBEAD-LOADED HYDROGEL FOR TREATMENT OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA IN A MOUSE MODEL OF BONE INFECTION

Author

Chitto, M., primary, Chen, B., additional, Kunisch, F., additional, Wychowaniec, J., additional, Onsea, J., additional, Post, V., additional, Richards, G., additional, Zeiter, S., additional, Wagemans, J., additional, Trampuz, A., additional, D'Este, M., additional, Moreno, M., additional, Lavigne, R., additional, and Moriarty, F., additional

Published
2023
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12. Ckp1 Bacteriophage, a S16-Like Broad-Host-Range Myovirus, Recognizes Citrobacter Koseri Lipopolysaccharide Through its Long Tail Fibers

Author

Oliveira, Hugo, primary, Santos, Sílvio, additional, Pires, Diana P., additional, Boeckaerts, Dimitri, additional, Pinto, Graça, additional, Domingues, Rita, additional, Otero, Jennifer, additional, Briers, Yves, additional, Lavigne, R., additional, Schmelcher, Mathias, additional, Dötsch, Andreas, additional, and Azeredo, Joana, additional

Published
2022
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14. Isolation and Characterization of a Thermophilic Chain Elongating Bacterium that Produces the High Commodity Chemical N-Caproate from Polymeric Carbohydrates

Author

Nguyen, Tinh Van, primary, Viver, Tomeu, additional, Mortier, Jonah, additional, Liu, Bin, additional, Smets, Ilse, additional, Bernaerts, Kristel, additional, Faust, Karoline, additional, Lavigne, R., additional, Poughon, Laurent, additional, Dussap, Claude-Gilles, additional, and Springael, Dirk, additional

Published
2022
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15. Tail-Tape-Fused Virion and Non-Virion RNA Polymerases of a Thermophilic Virus

Author

Chaban, Anastasiia, primary, Minakhin, Leonid, additional, Goldobina, Ekaterina, additional, Bae, Brain, additional, Hao, Yue, additional, Borukhov, Sergei, additional, Putzeys, Leena, additional, Boon, Maarten, additional, Lavigne, R., additional, Makarova, Kira S., additional, Koonin, Eugene, additional, Nair, Satish K., additional, Tagami, Shunsuke, additional, Severinov, Konstantin, additional, and Sokolova, Maria L., additional

Published
2022
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20. DOSSIER : Bestrijding bacterieziekten met faagbiocontrole

Author

Wagemans, J., Holtappels, D., Fortuna, K., Lavigne, R., Olaerts, A., Van Vaerenbergh, J., Peeters, K., Tyvaert, L., Wagemans, J., Holtappels, D., Fortuna, K., Lavigne, R., Olaerts, A., Van Vaerenbergh, J., Peeters, K., and Tyvaert, L.

Abstract

"Virussen zijn biologisch alternatief voor gewasbeschermingsmiddelen" : KU Leuven en ILVO voeren onderzoek naar gewasbescherming met fagen ; "Gekke wortels overwinnen is geen hopeloze zaak" : ILVO-onderzoeker Johan Van Vaerenbergh strijdt tegen gekke wortels ; Resultaten van enquête 'Bestijding van gekke wortels' ; 8 vragen over fagen ; Brug tussen onderzoek en praktijk.

Published
2021

29. Caractérisation protéomique du liquide coelomique de salmonidés

Author

Guého, Aurélie, Rime, Hélène, Com, Emmanuelle, Lavigne, R., Guével, B., Montfort, Jérôme, Labbé, Catherine, Pineau, Charles, Bobe, Julien, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

30. Analysis of Spounaviruses as a Case Study for the Overdue Reclassification of Tailed Phages

Author

Barylski, J., Enault, F., Dutilh, B.E., Schuller, M.B., Edwards, R.A., Gillis, A., Klumpp, J., Knezevic, P., Krupovic, M., Kuhn, J.H., Lavigne, R., Oksanen, H.M., Sullivan, M.B., Jang, H.B., Simmonds, P., Aiewsakun, P., Wittmann, J., Tolstoy, I., Brister, J.R., Kropinski, A.M., Adriaenssens, E.M., Barylski, J., Enault, F., Dutilh, B.E., Schuller, M.B., Edwards, R.A., Gillis, A., Klumpp, J., Knezevic, P., Krupovic, M., Kuhn, J.H., Lavigne, R., Oksanen, H.M., Sullivan, M.B., Jang, H.B., Simmonds, P., Aiewsakun, P., Wittmann, J., Tolstoy, I., Brister, J.R., Kropinski, A.M., and Adriaenssens, E.M.

Abstract

Contains fulltext : 218209.pdf (publisher's version ) (Open Access), Tailed bacteriophages are the most abundant and diverse viruses in the world, with genome sizes ranging from 10 kbp to over 500 kbp. Yet, due to historical reasons, all this diversity is confined to a single virus order-Caudovirales, composed of just four families: Myoviridae, Siphoviridae, Podoviridae, and the newly created Ackermannviridae family. In recent years, this morphology-based classification scheme has started to crumble under the constant flood of phage sequences, revealing that tailed phages are even more genetically diverse than once thought. This prompted us, the Bacterial and Archaeal Viruses Subcommittee of the International Committee on Taxonomy of Viruses (ICTV), to consider overall reorganization of phage taxonomy. In this study, we used a wide range of complementary methods-including comparative genomics, core genome analysis, and marker gene phylogenetics-to show that the group of Bacillus phage SPO1-related viruses previously classified into the Spounavirinae subfamily, is clearly distinct from other members of the family Myoviridae and its diversity deserves the rank of an autonomous family. Thus, we removed this group from the Myoviridae family and created the family Herelleviridae-a new taxon of the same rank. In the process of the taxon evaluation, we explored the feasibility of different demarcation criteria and critically evaluated the usefulness of our methods for phage classification. The convergence of results, drawing a consistent and comprehensive picture of a new family with associated subfamilies, regardless of method, demonstrates that the tools applied here are particularly useful in phage taxonomy. We are convinced that creation of this novel family is a crucial milestone toward much-needed reclassification in the Caudovirales order.

Published
2020

31. Taxonomy of prokaryotic viruses: 2018-2019 update from the ICTV Bacterial and Archaeal Viruses Subcommittee.

Author

Adriaenssens, EM, Sullivan, MB, Knezevic, P, van Zyl, LJ, Sarkar, BL, Dutilh, BE, Alfenas-Zerbini, P, Łobocka, M, Tong, Y, Brister, JR, Moreno Switt, AI, Klumpp, J, Aziz, RK, Barylski, J, Uchiyama, J, Edwards, RA, Kropinski, AM, Petty, NK, Clokie, MRJ, Kushkina, AI, Morozova, VV, Duffy, S, Gillis, A, Rumnieks, J, Kurtböke İ, Chanishvili, N, Goodridge, L, Wittmann, J, Lavigne, R, Jang, HB, Prangishvili, D, Enault, F, Turner, D, Poranen, MM, Oksanen, HM, Krupovic, M, Adriaenssens, EM, Sullivan, MB, Knezevic, P, van Zyl, LJ, Sarkar, BL, Dutilh, BE, Alfenas-Zerbini, P, Łobocka, M, Tong, Y, Brister, JR, Moreno Switt, AI, Klumpp, J, Aziz, RK, Barylski, J, Uchiyama, J, Edwards, RA, Kropinski, AM, Petty, NK, Clokie, MRJ, Kushkina, AI, Morozova, VV, Duffy, S, Gillis, A, Rumnieks, J, Kurtböke İ, Chanishvili, N, Goodridge, L, Wittmann, J, Lavigne, R, Jang, HB, Prangishvili, D, Enault, F, Turner, D, Poranen, MM, Oksanen, HM, and Krupovic, M

Abstract

This article is a summary of the activities of the ICTV's Bacterial and Archaeal Viruses Subcommittee for the years 2018 and 2019. Highlights include the creation of a new order, 10 families, 22 subfamilies, 424 genera and 964 species. Some of our concerns about the ICTV's ability to adjust to and incorporate new DNA- and protein-based taxonomic tools are discussed.

Published
2020

36. Host metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation

Author

Hendrix, H., primary, Kogadeeva, M., additional, Zimmermann, M., additional, Sauer, U., additional, De Smet, J., additional, Muchez, L., additional, Lissens, M., additional, Staes, I., additional, Voet, M., additional, Wagemans, J., additional, Ceyssens, P-J., additional, Noben, J-P., additional, Aertsen, A., additional, and Lavigne, R., additional

Published
2019
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38. Minimum Information about an Uncultivated Virus Genome (MIUViG)

Author

Roux, S., Adriaenssens, E.M., Dutilh, B.E., Koonin, E.V., Kropinski, A.M., Krupovic, M., Kuhn, J.H., Lavigne, R., Brister, J.R., Varsani, A., Amid, C., Aziz, R.K., Bordenstein, S.R., Bork, P., Breitbart, M., Cochrane, G.R., Daly, R.A., Desnues, C., Duhaime, M.B., Emerson, J.B., Enault, F., Fuhrman, J.A., Hingamp, P., Hugenholtz, P., Hurwitz, B.L., Ivanova, N.N., Labonte, J.M., Lee, K.B., Malmstrom, R.R., Martinez-Garcia, M., Mizrachi, I.K., Ogata, H., Paez-Espino, D., Petit, M.A., Putonti, C., Rattei, T., Reyes, A., Rodriguez-Valera, F., Rosario, K., Schriml, L., Schulz, F., Steward, G.F., Sullivan, M.B., Sunagawa, S., Suttle, C.A., Temperton, B., Tringe, S.G., Thurber, R.V., Webster, N.S., Whiteson, K.L., Wilhelm, S.W., Wommack, K.E., Woyke, T., Wrighton, K.C., Yilmaz, P., Yoshida, T., Young, M.J., Yutin, N., Allen, L.Z., Kyrpides, N.C., Eloe-Fadrosh, E.A., Roux, S., Adriaenssens, E.M., Dutilh, B.E., Koonin, E.V., Kropinski, A.M., Krupovic, M., Kuhn, J.H., Lavigne, R., Brister, J.R., Varsani, A., Amid, C., Aziz, R.K., Bordenstein, S.R., Bork, P., Breitbart, M., Cochrane, G.R., Daly, R.A., Desnues, C., Duhaime, M.B., Emerson, J.B., Enault, F., Fuhrman, J.A., Hingamp, P., Hugenholtz, P., Hurwitz, B.L., Ivanova, N.N., Labonte, J.M., Lee, K.B., Malmstrom, R.R., Martinez-Garcia, M., Mizrachi, I.K., Ogata, H., Paez-Espino, D., Petit, M.A., Putonti, C., Rattei, T., Reyes, A., Rodriguez-Valera, F., Rosario, K., Schriml, L., Schulz, F., Steward, G.F., Sullivan, M.B., Sunagawa, S., Suttle, C.A., Temperton, B., Tringe, S.G., Thurber, R.V., Webster, N.S., Whiteson, K.L., Wilhelm, S.W., Wommack, K.E., Woyke, T., Wrighton, K.C., Yilmaz, P., Yoshida, T., Young, M.J., Yutin, N., Allen, L.Z., Kyrpides, N.C., and Eloe-Fadrosh, E.A.

Abstract

Contains fulltext : 205177.pdf (publisher's version ) (Open Access), We present an extension of the Minimum Information about any (x) Sequence (MIxS) standard for reporting sequences of uncultivated virus genomes. Minimum Information about an Uncultivated Virus Genome (MIUViG) standards were developed within the Genomic Standards Consortium framework and include virus origin, genome quality, genome annotation, taxonomic classification, biogeographic distribution and in silico host prediction. Community-wide adoption of MIUViG standards, which complement the Minimum Information about a Single Amplified Genome (MISAG) and Metagenome-Assembled Genome (MIMAG) standards for uncultivated bacteria and archaea, will improve the reporting of uncultivated virus genomes in public databases. In turn, this should enable more robust comparative studies and a systematic exploration of the global virosphere.

Published
2019

39. Phage Genome Annotation Using the RAST Pipeline

Author

McNair, Katelyn, Aziz, Ramy Karam, Pusch, Gordon D, Overbeek, Ross, Dutilh, Bas E, Edwards, Robert A, Clokie, M.R.J., Kropinski, A.M., Lavigne, R., Sub Bioinformatics, and Theoretical Biology and Bioinformatics

Subjects
RAST, Coronacrisis-Taverne, Phage, Functional annotation, Gene predictions, Genome annotation
Abstract

Phages are complex biomolecular machineries that have to survive in a bacterial world. Phage genomes show many adaptations to their lifestyle such as shorter genes, reduced capacity for redundant DNA sequences, and the inclusion of tRNAs in their genomes. In addition, phages are not free-living, they require a host for replication and survival. These unique adaptations provide challenges for the bioinformatics analysis of phage genomes. In particular, ORF calling, genome annotation, noncoding RNA (ncRNA) identification, and the identification of transposons and insertions are all complicated in phage genome analysis. We provide a road map through the phage genome annotation pipeline, and discuss the challenges and solutions for phage genome annotation as we have implemented in the rapid annotation using subsystems (RAST) pipeline.

Published
2018

40. Could bacteriophages help with managing cholera in The Democratic Republic of Congo (DRC)?

Author

Maestri, A., primary, Chan, B., additional, de Vos, D., additional, Pirnay, J.-P., additional, Turner, P., additional, Zozo, D., additional, Lavigne, R., additional, Nagel, T., additional, Kamiwiziku, G., additional, Batumbo, D., additional, Bompangue, D., additional, Merabishvili, M., additional, Kakabadze, E., additional, and Gribaudo, G., additional

Published
2018
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41. Taxonomy of prokaryotic viruses: 2017 update from the ICTV Bacterial and Archaeal Viruses Subcommittee

Author

Adriaenssens, E.M., Wittmann, J., Kuhn, J.H., Turner, D., Sullivan, M.B., Dutilh, B.E., Jang, H.B., Zyl, L.J. van, Klumpp, J., Lobocka, M., Switt, A.I. Moreno, Rumnieks, J., Edwards, R.A., Uchiyama, J., Alfenas-Zerbini, P., Petty, N.K., Kropinski, A.M., Barylski, J., Gillis, A., Clokie, M.R., Prangishvili, D., Lavigne, R., Aziz, R.K., Duffy, S., Krupovic, M., Poranen, M.M., Knezevic, P., Enault, F., Tong, Y., Oksanen, H.M., Brister, J. Rodney, Adriaenssens, E.M., Wittmann, J., Kuhn, J.H., Turner, D., Sullivan, M.B., Dutilh, B.E., Jang, H.B., Zyl, L.J. van, Klumpp, J., Lobocka, M., Switt, A.I. Moreno, Rumnieks, J., Edwards, R.A., Uchiyama, J., Alfenas-Zerbini, P., Petty, N.K., Kropinski, A.M., Barylski, J., Gillis, A., Clokie, M.R., Prangishvili, D., Lavigne, R., Aziz, R.K., Duffy, S., Krupovic, M., Poranen, M.M., Knezevic, P., Enault, F., Tong, Y., Oksanen, H.M., and Brister, J. Rodney

Abstract

Item does not contain fulltext

Published
2018

42. Targeting mechanisms of tailed bacteriophages

Author

Nobrega, F.L., Vlot, M., Jonge, P.A. de, Dreesens, L.L., Beaumont, H.J.E., Lavigne, R., Dutilh, B.E., Brouns, S.J., Nobrega, F.L., Vlot, M., Jonge, P.A. de, Dreesens, L.L., Beaumont, H.J.E., Lavigne, R., Dutilh, B.E., and Brouns, S.J.

Abstract

Contains fulltext : 200432.pdf (publisher's version ) (Closed access), Phages differ substantially in the bacterial hosts that they infect. Their host range is determined by the specific structures that they use to target bacterial cells. Tailed phages use a broad range of receptor-binding proteins, such as tail fibres, tail spikes and the central tail spike, to target their cognate bacterial cell surface receptors. Recent technical advances and new structure-function insights have begun to unravel the molecular mechanisms and temporal dynamics that govern these interactions. Here, we review the current understanding of the targeting machinery and mechanisms of tailed phages. These new insights and approaches pave the way for the application of phages in medicine and biotechnology and enable deeper understanding of their ecology and evolution.

Published
2018

43. Phage Genome Annotation Using the RAST Pipeline

Author

Sub Bioinformatics, Theoretical Biology and Bioinformatics, McNair, Katelyn, Aziz, Ramy Karam, Pusch, Gordon D, Overbeek, Ross, Dutilh, Bas E, Edwards, Robert A, Clokie, M.R.J., Kropinski, A.M., Lavigne, R., Sub Bioinformatics, Theoretical Biology and Bioinformatics, McNair, Katelyn, Aziz, Ramy Karam, Pusch, Gordon D, Overbeek, Ross, Dutilh, Bas E, Edwards, Robert A, Clokie, M.R.J., Kropinski, A.M., and Lavigne, R.

Published
2018

44. Taxonomy of prokaryotic viruses: 2017 update from the ICTV Bacterial and Archaeal Viruses Subcommittee

Author

Adriaenssens, EM, Wittmann, J, Kuhn, JH, Turner, D, Sullivan, MB, Dutilh, BE, Jang, HB, van Zyl, LJ, Klumpp, J, Lobocka, M, Moreno Switt, AI, Rumnieks, J, Edwards, RA, Uchiyama, J, Alfenas-Zerbini, P, Petty, NK, Kropinski, AM, Barylski, J, Gillis, A, Clokie, MRC, Prangishvili, D, Lavigne, R, Aziz, RK, Duffy, S, Krupovic, M, Poranen, MM, Knezevic, P, Enault, F, Tong, Y, Oksanen, HM, Rodney Brister, J, Adriaenssens, EM, Wittmann, J, Kuhn, JH, Turner, D, Sullivan, MB, Dutilh, BE, Jang, HB, van Zyl, LJ, Klumpp, J, Lobocka, M, Moreno Switt, AI, Rumnieks, J, Edwards, RA, Uchiyama, J, Alfenas-Zerbini, P, Petty, NK, Kropinski, AM, Barylski, J, Gillis, A, Clokie, MRC, Prangishvili, D, Lavigne, R, Aziz, RK, Duffy, S, Krupovic, M, Poranen, MM, Knezevic, P, Enault, F, Tong, Y, Oksanen, HM, and Rodney Brister, J

Published
2018

45. Activity of bacteriophages to multiply resistant strains of salmonella and their various serotypes

Author

Makalatia, Kh., primary, Kakabadze, E., additional, Bakuradze, N., additional, Grdzelishvili, N., additional, Natroshvili, G., additional, Kusradze, I., additional, Goderdzishvili, M., additional, Sedrakyan, A., additional, Arakelova, K., additional, Mkrtchyan, M., additional, Macharashvili, N., additional, Papiashvili, E., additional, Lavigne, R., additional, Lee, D., additional, Coffey, A., additional, De Vos, D., additional, Pirnay, J. P., additional, and Chanishvili, N., additional

Published
2018
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47. Minimum Information about a Biosynthetic Gene cluster

Author

Medema, M., Kottmann, R., Yilmaz, P., Cummings, M., Biggins, J., Blin, K., de Bruijn, I., Chooi, Y., Claesen, J., Coates, R., Cruz-Morales, P., Duddela, S., Düsterhus, S., Edwards, D., Fewer, D., Garg, N., Geiger, C., Gomez-Escribano, J., Greule, A., Hadjithomas, M., Haines, A., Helfrich, E., Hillwig, M., Ishida, K., Jones, A., Jones, C., Jungmann, K., Kegler, C., Uk Kim, H., Kötter, P., Krug, D., Masschelein, J., Melnik, A., Mantovani, S., Monroe, E., Moore, M., Moss, N., Nützmann, H., Pan, G., Pati, A., Petras, D., Reen, F., Rui, Z., Tian, Z., Tsunematsu, Y., Tobias, N., Wiemann, P., Wyckoff, E., Yan, X., Yim, G., Yu, F., Xie, Y., Aigle, B., Apel, A., Balibar, C., Balskus, E., Barona-Gómez, F., Bechthold, A., Bode, H., Borriss, R., Brady, S., Brakhage, A., Caffrey, P., Cheng, Y., Clardy, J., Cox, R., De Mot, R., Donadio, S., van der Donk, W., Dorrestein, P., Doyle, S., Driessen, A., Ehling-Schulz, M., Entian, K., Fischbach, M., Gerwick, L., Gerwick, W., Gross, H., Gust, B., Hertweck, C., Höfte, M., Jensen, S., Ju, J., Katz, L., Kaysser, L., Klassen, J., Keller, N., Kormanec, J., Kuipers, O., Kuzuyama, T., Kyrpides, N., Kwon, H., Lautru, S., Lavigne, R., Lee, C., Linquan, B., Liu, X., Liu, W., Luzhetskyy, A., Mahmud, T., Mast, Y., Méndez, C., Metsä-Ketelä, M., Micklefield, J., Mitchell, D., Moore, B., Moreira, L., Müller, R., Neilan, B., Nett, M., Nielsen, J., O’Gara, F., Oikawa, H., Osbourn, A., Osburne, M., Ostash, B., Payne, S., Pernode, J., Petricek, M., Piel, J., Ploux, O., Raaijmakers, J., Salas, J., Schmitt, E., Scott, B., Seipke, R., Shen, B., Sherman, D., Sivonen, K., Smanski, M., Sosio, M., Stegmann, E., Süssmuth, R., Tahlan, K., Thomas, C., Tang, Y., Truman, A., Viaud, M., Walton, J., Walsh, C., Weber, T., van Wezel, G., Wilkinson, B., Willey, J., Wohlleben, W., Wright, G., Ziemert, N., Zhang, C., Zotchev, S., Breitling, R., Takano, E., and Glöckner, F.

Abstract

A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters, pathways and metabolites is currently dispersed throughout the literature, making it difficult to exploit. To facilitate consistent and systematic deposition and retrieval of data on biosynthetic gene clusters, we propose the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard.

Published
2015

48. In vitro and in vivo assessment of phage therapy against Staphylococcus aureuscausing bovine mastitis

Author

Ngassam-Tchamba, C., Duprez, J.N., Fergestad, M., De Visscher, A., L’Abee-Lund, T., De Vliegher, S., Wasteson, Y., Touzain, F., Blanchard, Y., Lavigne, R., Chanishvili, N., Cassart, D., Mainil, J., and Thiry, D.

Abstract

•Lytic activities of three bacteriophages in vitro against bovine mastitis Staphylococcus aureus.•Survival rate below 50% in vivo at DPI 4 with Galleria mellonellalarvae.•Incomplete recovery of the mouse mastitis in vivo at HPI 48.•Significant differences after blending of larvae and mice mammary glands.

Published
2020
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49. Klebsiella phages representing a novel clade of viruses with an unknown DNA modification and biotechnologically interesting enzymes

Author

Maciejewska, B, Roszniowski, B, Espaillat, Akbar, Kęsik-Szeloch, A, Majkowska-Skrobek, G, Kropinski, AM, Briers, Y, Cava, Felipe, Lavigne, R, Drulis-Kawa, Z, Maciejewska, B, Roszniowski, B, Espaillat, Akbar, Kęsik-Szeloch, A, Majkowska-Skrobek, G, Kropinski, AM, Briers, Y, Cava, Felipe, Lavigne, R, and Drulis-Kawa, Z

Abstract

Lytic bacteriophages and phage-encoded endolysins (peptidoglycan hydrolases) provide a source for the development of novel antimicrobial strategies. In the present study, we focus on the closely related (96 % DNA sequence identity) environmental myoviruses vB_KpnM_KP15 (KP15) and vB_KpnM_KP27 (KP27) infecting multidrug-resistant Klebsiella pneumoniae and Klebsiella oxytoca strains. Their genome organisation and evolutionary relationship are compared to Enterobacter phage phiEap-3 and Klebsiella phages Matisse and Miro. Due to the shared and distinct evolutionary history of these phages, we propose to create a new phage genus BKp15virus^ within the Tevenvirinae subfamily. In silico genome analysis reveals two unique putative homing endonucleases of KP27 phage, probably involved in unrevealed mechanism of DNA modification and resistance to restriction digestion, resulting in a broader host spectrum. Additionally, we identified in KP15 and KP27 a complete set of lysis genes, containing holin, antiholin, spanin and endolysin. By turbidimetric assays on permeabilized Gram-negative strains, we verified the ability of the KP27 endolysin to destroy the bacterial peptidoglycan. We confirmed high stability, absence of toxicity on a human epithelial cell line and the enzymatic specificity of endolysin, which was found to possess endopeptidase activity, cleaving the peptide stem between L-alanine and D-glutamic acid.

Published
2017
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50. Taxonomy of prokaryotic viruses: 2016 update from the ICTV bacterial and archaeal viruses subcommittee

Author

Adriaenssens, E.M., Krupovic, M., Knezevic, P., Ackermann, H.W., Barylski, J., Brister, J.R., Clokie, M.R., Duffy, S., Dutilh, B.E., Edwards, R.A., Enault, F., Jang, H.B., Klumpp, J., Kropinski, A.M., Lavigne, R., Poranen, M.M., Prangishvili, D., Rumnieks, J., Sullivan, M.B., Wittmann, J., Oksanen, H.M., Gillis, A., Kuhn, J.H., Adriaenssens, E.M., Krupovic, M., Knezevic, P., Ackermann, H.W., Barylski, J., Brister, J.R., Clokie, M.R., Duffy, S., Dutilh, B.E., Edwards, R.A., Enault, F., Jang, H.B., Klumpp, J., Kropinski, A.M., Lavigne, R., Poranen, M.M., Prangishvili, D., Rumnieks, J., Sullivan, M.B., Wittmann, J., Oksanen, H.M., Gillis, A., and Kuhn, J.H.

Abstract

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Published
2017

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