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8. Effects of Active-Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O-Methyltransferase.

Author

Law BJ, Bennett MR, Thompson ML, Levy C, Shepherd SA, Leys D, and Micklefield J

Subjects
Catalytic Domain, Catechol O-Methyltransferase chemistry, Crystallography, X-Ray, Protein Structure, Quaternary, Catechol O-Methyltransferase metabolism
Abstract

Catechol-O-methyltransferase (COMT), an important therapeutic target in the treatment of Parkinson's disease, is also being developed for biocatalytic processes, including vanillin production, although lack of regioselectivity has precluded its more widespread application. By using structural and mechanistic information, regiocomplementary COMT variants were engineered that deliver either meta- or para-methylated catechols. X-ray crystallography further revealed how the active-site residues and quaternary structure govern regioselectivity. Finally, analogues of AdoMet are accepted by the regiocomplementary COMT mutants and can be used to prepare alkylated catechols, including ethyl vanillin., (© 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)

Published
2016
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