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2. Manuka honey microneedles for enhanced wound healing and the prevention and/or treatment of Methicillin-resistant Staphylococcus aureus (MRSA) surgical site infection

Author

Massachusetts Institute of Technology. Division of Comparative Medicine, Massachusetts Institute of Technology. Department of Biological Engineering, Frydman, Galit H., Olaleye, David, Annamalai, Damodaran, Layne, Kim, Yang, Illina, Kaafarani, Haytham MA, Fox, James G, Massachusetts Institute of Technology. Division of Comparative Medicine, Massachusetts Institute of Technology. Department of Biological Engineering, Frydman, Galit H., Olaleye, David, Annamalai, Damodaran, Layne, Kim, Yang, Illina, Kaafarani, Haytham MA, and Fox, James G

Abstract

© 2020, The Author(s). Manuka honey (MH) is currently used as a wound treatment and suggested to be effective in Methicillin-resistant Staphylococcus aureus (MRSA) elimination. We sought to optimize the synthesis of MH microneedles (MHMs) while maintaining the MH therapeutic effects. MHMs were synthesized using multiple methods and evaluated with in vitro assays. MHMs demonstrated excellent bactericidal activity against MRSA at concentrations ≥ 10% of honey, with vacuum-prepared honey appearing to be the most bactericidal, killing bacterial concentrations as high as 8 × 107 CFU/mL. The wound-healing assay demonstrated that, at concentrations of 0.1%, while the cooked honey had incomplete wound closure, the vacuum-treated honey trended towards faster wound closure. In this study, we demonstrate that the method of MHM synthesis is crucial to maintaining MH properties. We optimized the synthesis of MHMs and demonstrated their potential utility in the treatment of MRSA infections as well as in wound healing. This is the first report of using MH as a substrate for the formation of dissolvable microneedles. This data supports the need for further exploration of this new approach in a wound-healing model and opens the door for the future use of MH as a component of microneedle scaffolds., NIH (Grants P30-ES002109 (JGF), P50-GM021700)

Published
2021

3. Manuka honey microneedles for enhanced wound healing and the prevention and/or treatment of Methicillin-resistant Staphylococcus aureus (MRSA) surgical site infection

Author

Frydman, Galit H, Olaleye, David, Annamalai, Damodaran, Layne, Kim, Yang, Illina, Kaafarani, Haytham MA, Fox, James G, Frydman, Galit H, Olaleye, David, Annamalai, Damodaran, Layne, Kim, Yang, Illina, Kaafarani, Haytham MA, and Fox, James G

Abstract

© 2020, The Author(s). Manuka honey (MH) is currently used as a wound treatment and suggested to be effective in Methicillin-resistant Staphylococcus aureus (MRSA) elimination. We sought to optimize the synthesis of MH microneedles (MHMs) while maintaining the MH therapeutic effects. MHMs were synthesized using multiple methods and evaluated with in vitro assays. MHMs demonstrated excellent bactericidal activity against MRSA at concentrations ≥ 10% of honey, with vacuum-prepared honey appearing to be the most bactericidal, killing bacterial concentrations as high as 8 × 107 CFU/mL. The wound-healing assay demonstrated that, at concentrations of 0.1%, while the cooked honey had incomplete wound closure, the vacuum-treated honey trended towards faster wound closure. In this study, we demonstrate that the method of MHM synthesis is crucial to maintaining MH properties. We optimized the synthesis of MHMs and demonstrated their potential utility in the treatment of MRSA infections as well as in wound healing. This is the first report of using MH as a substrate for the formation of dissolvable microneedles. This data supports the need for further exploration of this new approach in a wound-healing model and opens the door for the future use of MH as a component of microneedle scaffolds.

Published
2021

4. Normal subjects consuming physiological levels of 18:3(n-3) and 20:5(n-3) from flaxseed or fish oils have characteristic differences in plasma lipid and lipoprotein fatty acid levels

Author

Layne, Kim S., Goh, Yeow K., Jumpsen, Jacqueline A., Ryan, Edmond A., Chow, Peter, and Clandinin, Michael T.

Subjects
Flaxseed -- Health aspects, Fish oils -- Health aspects, Fatty acids -- Physiological aspects, Blood lipids -- Physiological aspects, Blood cholesterol -- Physiological aspects, Food/cooking/nutrition
Abstract

The study assessed the effect of low doses of fatty acids from fish or flaxseed oil on plasma lipid concentrations in normal humans consuming diets with either high (0.87, n = 11) or low (0.48, n = 15) dietary polyunsaturated/saturated fatty acid (P/S) ratios. The dose of (n-3) fatty acids reflected an (n-3) intake that could easily be attained by selection of foods in a normal diet. The individuals were initially supplemented with olive oil [35 mg 18:1/(kg body weight. d)], and then were randomly assigned to either flaxseed or fish oil [35 mg 18:3(n-3) or 35 mg 20:5(n-3) + 22:6(n-3)/(kg body weight [multiplied by] d), respectively] treatments. Participants consumed each oil supplement for 3 mo. Blood samples were drawn for analysis at the end of each 3-mo period. Plasma triacylglycerol, total, LDL and HDL cholesterol concentrations, and lipoprotein fatty acid concentrations are shown. Fish oil reduced plasma triacylglycerol and increased lipo-protein levels of 20:5(n-3) and 22:6(n-3). The flaxseed oil did not alter plasma triacylglycerol level and produced small changes in 20:5(n-3) and 22:6(n-3) concentrations. Total, LDL and HDL cholesterol levels were not affected by either (n-3) fatty acid. Significant differences in plasma triacylglycerol concentrations and total and LDL cholesterol levels were found between the two dietary P/S groups after all oil treatment periods. Levels of 18:3(n-3), 20:4(n-6), 20:5(n-3), and 22:6(n-3) in LDL were also different in high vs. low dietary P/S groups for all oil treatments and in the VLDL for the olive oil and fish oil supplementation. This study indicates that low intake of purified fish oil induces changes in plasma triacylglycerol, 20:5(n-3) levels in VLDL, LDL, and HDL, and 22:6(n-3) levels in LDL and HDL that are apparent after 3 mo and which might influence atherogenicity of lipoprotein particles in normal free-living individuals. J. Nutr. 126: 2130-2140, 1996. INDEXING KEY WORDS: * dietary fatty acids * plasma lipoprotein * phospholipid * cholesterol * humans

Published
1996

7. Normal subjects consuming physiological levels of 18:3 (n-3) and 20:5 (n-3) from flaxseed or fish...

Author

Layne, Kim S. and Goh, Yeow K.

Subjects
*FISH oils, *FATTY acids, *BLOOD lipids, *PHYSIOLOGY
Abstract

Assesses the effect of low doses of fatty acids from fish or flaxseed oil on plasma lipid concentration in normal individuals. Reduced plasma triacylglycerol on low-dose fish oil; Maintenance of hypotriacylglyceridemic effects of marine (n-3) fatty acids for 3 months; Effect of (n-3) fatty acids supplementation.

Published
1996
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