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Objective: Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by increasing prevalence, diverse impairments, and unclear origins and mechanisms. To gain a better grasp of the origins of ASD, it is essential to identify the most distinctive structural brain abnormalities in individuals with ASD. Methods: A Multi-Stage Progressive Feature Refinement Approach was employed to identify the most pivotal structural magnetic resonance imaging (MRI) features that distinguish individuals with ASD from typically developing (TD) individuals. The study included 175 individuals with ASD and 69 TD individuals, all aged between 7 and 18 years, matched in terms of age and gender. Both cortical and subcortical features were integrated, with a particular focus on hippocampal subfields. Results: Out of 317 features, 9 had the most significant impact on distinguishing ASD from TD individuals. These structural features, which include a specific hippocampal subfield, are closely related to the brain areas associated with the reward system. Conclusion: Structural irregularities in the reward system may play a crucial role in the pathophysiology of ASD, and specific hippocampal subfields may also contribute uniquely, warranting further investigation. [ABSTRACT FROM AUTHOR]

Predicting crop yields before harvest is important for making and carrying out policies about food safety, transportation costs, import-export, storage, and selling of agricultural goods. The weather is a key factor in crop growth and its development. Therefore, models that include meteorological variables can predict reliable forecasts for crop output; however, selecting the appropriate model for use in agricultural production forecasting can be challenging. This study investigates the development of wheat yield prediction models using various multivariate analysis techniques and weather indices derived from meteorological data collected over 22 years in Punjab, India. Five different modeling approaches, including stepwise multiple linear regression (SMLR), LASSO, elastic net (ELNET), artificial neural network (ANN), and ridge regression, were employed and compared for their effectiveness in predicting wheat yield. The models were calibrated using data from 17 years (2000–01 to 2016–17) and validated using data from the subsequent 5 years (2017–18 to 2021–22). Evaluation metrics such as R2, root mean square error (RMSE), normalized root mean square error (NRMSE), mean biased error (MBE), and modeling efficiency (EF) were utilized to assess model performance. The results indicate varying degrees of performance across districts and modeling techniques. ANN demonstrated the highest performance during both calibration and validation periods, followed closely by LASSO and ELNET. However, certain districts showed discrepancies in model fit, with some models performing better than others depending on the specific district. Overall, ANN emerged as the most reliable approach for wheat yield prediction in Punjab followed by ELNET and LASSO, offering valuable insights for agricultural planning and management. This comprehensive analysis provides valuable contributions to the field of crop yield prediction, enhancing understanding of the complex interactions between weather variables and agricultural outcomes. [ABSTRACT FROM AUTHOR]

Background: Colon adenocarcinoma (COAD) is a gastrointestinal malignancy with a high mortality rate. Studies have confirmed the role of immunogenic cell death (ICD) in different cancer types. However, there is a lack of research on ICD-related genes (ICD-RGs) in COAD. This study aimed to examine the impact of ICD-RGs on COAD and their interaction with the immune microenvironment. Methods: Using data from The Cancer Genome Atlas and Gene Expression Omnibus databases, we identified 107 ICD-RGs in COAD. Using a one-way Cox regression analysis, we examined the relationship between these ICD-RGs and overall survival in COAD. Results: Following the regression analyses, we identified 14 overall survival-related genes. Furthermore, we examined the predictive impact of the ICD-RGs using the least absolute shrinkage and selection operator regression analysis and developed a nine-genes prognostic model. The Cancer Genome Atlas and Gene Expression Omnibus datasets were used for training and validation. Kaplan-Meier analysis was used to confirm that the high-risk group had a lower survival rate than the low-risk group. Finally, following a multifactorial analysis, we created a prognostic nomogram that integrated clinical data and risk scores. Conclusions: The nine-genes model exhibits robust stability and can provide valuable insights for guiding the development of tumor immunotherapy strategies and personalized drug selection for patients with COAD. [ABSTRACT FROM AUTHOR]

Introduction: The global burden of multiple myeloma (MM) is increasing every year. Here, we have developed machine learning models to provide a reference for the early detection of MM. Methods: A total of 465 patients and 150 healthy controls were enrolled in this retrospective study. Based on the variable screening strategy of least absolute shrinkage and selection operator (LASSO), three prediction models, logistic regression (LR), support vector machine (SVM), and random forest (RF), were established combining complete blood count (CBC) and cell population data (CPD) parameters in the training set (210 cases), and were verified in the validation set (90 cases) and test set (165 cases). The performance of each model was analyzed using receiver operating characteristic (ROC) curve, calibration curves, and decision curve analysis (DCA). Accuracy, sensitivity, specificity, positive predictive value, negative predictive value, and area under the ROC curve (AUC) were applied to evaluate the models. Delong test was used to compare the AUC of the models. Results: Six parameters including RBC (1012/L), RDW‐CV (%), IG (%), NE‐WZ, LY‐WX, and LY‐WZ were screened out by LASSO to construct the model. Among the three models, the AUC of RF model in the training set, validation set, and test set were 0.956, 0.892, and 0.875, which were higher than those of LR model (0.901, 0.849, and 0.858) and SVM model (0.929, 0.868, and 0.846). Delong test showed that there were significant differences among the models in the training set, no significant differences in the validation set, and significant differences only between SVM and RF models in the test set. The calibration curve and DCA showed that the three models had good validity and feasibility, and the RF model performed best. Conclusion: The proposed RF model may be a useful auxiliary tool for rapid screening of MM patients. [ABSTRACT FROM AUTHOR]

Background: Delayed hemothorax (dHTX) can occur unexpectedly, even in patients who initially present without signs of hemothorax (HTX), potentially leading to death. We aimed to develop a predictive model for dHTX requiring intervention, specifically targeting those with no or occult HTX. Methods: This retrospective study was conducted at a level 1 trauma center. The primary outcome was the occurrence of dHTX requiring intervention in patients who had no HTX or occult HTX and did not undergo closed thoracostomy post-injury. To minimize overfitting, we employed the least absolute shrinkage and selection operator (LASSO) logistic regression model for feature selection. Thereafter, we developed a multivariable logistic regression (MLR) model and a nomogram. Results: In total, 688 patients were included in the study, with 64 cases of dHTX (9.3%). The LASSO and MLR analyses revealed that the depth of HTX (adjusted odds ratio [aOR], 3.79; 95% confidence interval [CI], 2.10–6.85; p

BackgroundPostoperative respiratory failure (PRF) is associated with increased hospital charges and worse patient outcomes. Reliable prediction models can help to guide postoperative planning to optimize care, to guide resource allocation, and to foster shared decision-making with patients.Research questionCan a predictive model be developed to accurately identify patients at high risk of PRF?Study design and methodsIn this single-site proof-of-concept study, we used structured query language to extract, transform, and load electronic health record data from 23,999 consecutive adult patients admitted for elective surgery (2014-2021). Our primary outcome was PRF, defined as mechanical ventilation after surgery of > 48 h. Predictors of interest included demographics, comorbidities, and intraoperative factors. We used logistic regression to build a predictive model and the least absolute shrinkage and selection operator procedure to select variables and to estimate model coefficients. We evaluated model performance using optimism-corrected area under the receiver operating curve and area under the precision-recall curve and calculated sensitivity, specificity, positive and negative predictive values, and Brier scores.ResultsTwo hundred twenty-five patients (0.94%) demonstrated PRF. The 18-variable predictive model included: operations on the cardiovascular, nervous, digestive, urinary, or musculoskeletal system; surgical specialty orthopedic (nonspine); Medicare or Medicaid (as the primary payer); race unknown; American Society of Anesthesiologists class ≥ III; BMI of 30 to 34.9 kg/m2; anesthesia duration (per hour); net fluid at end of the operation (per liter); median intraoperative FIO2, end title CO2, heart rate, and tidal volume; and intraoperative vasopressor medications. The optimism-corrected area under the receiver operating curve was 0.835 (95% CI,0.808-0.862) and the area under the precision-recall curve was 0.156 (95% CI, 0.105-0.203).InterpretationThis single-center proof-of-concept study demonstrated that a structured query language extract, transform, and load process, based on readily available patient and intraoperative variables, can be used to develop a prediction model for PRF. This PRF prediction model is scalable for multicenter research. Clinical applications include decision support to guide postoperative level of care admission and treatment decisions.

BACKGROUND: Fibromyalgia syndrome, as a common rheumatic disease, is related to central sensitization and immune abnormalities. However, the specific mechanism has not been elucidated, and there is a lack of specific diagnostic markers. Exploring the possible pathogenesis of this disease has important clinical significance. OBJECTIVE: To screen the potential diagnostic marker genes of fibromyalgia syndrome and analyze the possible immune infiltration characteristics based on bioinformatics methods, such as weighted gene co-expression network analysis (WGCNA), and machine learning. METHODS: Gene expression profiles in peripheral serum of fibromyalgia syndrome patients and healthy controls were obtained from the gene expression omnibus (GEO) database. The differentially co-expressed genes were screened in the expression profile by differential analysis and WGCNA analysis. Least absolute shrinkage and selection operator (LASSO) and support vector machine -recursive feature elimination (SVM-RFE) machine learning algorithm were further used to identify hub biomarkers, and draw receiver operating characteristic curve (ROC) to evaluate the accuracy of diagnosing fibromyalgia syndrome. Finally, single sample gene set enrichment analysis (ssGSEA) and gene set enrichment analysis (GSEA) were used to evaluate the immune cell infiltration and pathway enrichment in patients with fibromyalgia syndrome. RESULTS AND CONCLUSION: Eight down-regulated differentially expressed genes (DEGs) were obtained after differential analysis of the GSE67311 dataset according to the conditions of log2|(FC)| > 0 and P < 0.05. After WGCNA analysis, 497 genes were included in the module (MEdarkviolet) with the highest positive correlation (r=0.22, P=0.04), and 19 genes were included in the module (MEsalmon2) with the highest negative correlation (r=-0.41, P=6×10-5). After intersecting DEGs and the module genes of WGCNA, seven genes were obtained. Four genes were screened out by LASSO regression algorithm and five genes were screened out by SVM-RFE machine learning algorithm. After the intersection of the two, three core genes were identified, which were germinal center associated signaling and motility like, integrin beta-8, and carboxypeptidase A3. The areas under the ROC curve of the three core genes were 0.744, 0.739, and 0.734, respectively, indicating that they have good diagnostic value and can be used as biomarkers for fibromyalgia syndrome. The results of immune infiltration analysis showed that memory B cells, CD56 bright NK cells, and mast cells were significantly down-regulated in patients with fibromyalgia syndrome compared with the control group (P < 0.05), and were significantly positively correlated with the above three biomarkers (P < 0.05). The enrichment analysis suggested that there were nine fibromyalgia syndrome enrichment pathways, mainly related to olfactory transduction pathway, neuroactive ligand- receptor interaction, and infection pathway. The above results showed that the occurrence and development of fibromyalgia syndrome are related to the involvement of multiple genes, abnormal immune regulation, and multiple pathways imbalance. However, the interactions between these genes and immune cells, as well as their relationships with various pathways need to be further investigated. [ABSTRACT FROM AUTHOR]

Background In recent years, numerous studies have indicated that hyperuricemia (HUA) is a contributing factor to certain diseases. However, whether HUA is a contributing factor to chronic pulmonary heart disease (CPHD) still requires further investigation. Objective To explore the association between HUA and CPHD, aiming to provide a theoretical basis for the management of serum uric acid (SUA) levels in patients with CPHD. Methods A total of1 171 patients with chronic obstructive pulmonary disease (COPD) admitted to the First Affiliated Hospital of Xinjiang Medical University from 2019 to 2023 were included in the study. They were divided into a CPHD group (470 cases) and a COPD group (701 cases) based on whether they had CPHD. General information, laboratory test results, and echocardiographic findings of the patients were collected. LASSO regression was used to select variables, and propensity score matching (PSM) was employed to eliminate the influence of confounding factors. Multivariate Logistic regression analysis was conducted to explore the influencing factors of CPHD in COPD patients. Results The CPHD group had lower proportions of females, Han ethnicity, smokers, drinkers, idiopathic pulmonary fibrosis, chronic bronchitis, bronchial asthma, lymphocyte percentage, left ventricular end-diastolic diameter, left ventricular end-systolic diameter, cardiac output, left ventricular ejection fraction compared to the COPD group. Higher proportions of heart function class 3-4, HUA, pulmonary embolism, congenital heart disease, red blood cell count, neutrophil percentage, SUA, blood urea nitrogen, D-dimer, N-terminal pro-B-type natriuretic peptide, right atrial diameter, right ventricular diameter, left atrial diameter, right ventricular outflow tract diameter, and pulmonary artery diameter were observed in the CPHD group, with statistically significant differences (P381.29 μmol/L) had a 1.421-fold inScrsed risk of having CPHD. Conclusion HUA is a contributing factor to the occurrence and development of CPHD. Actively controlling SUA levels may help prevent the occurrence and development of CPHD.

Introduction: The aim of this study was to compare various machine learning algorithms for constructing a diabetic retinopathy (DR) prediction model among type 2 diabetes mellitus (DM) patients and to develop a nomogram based on the best model. Methods: This cross-sectional study included DM patients receiving routine DR screening. Patients were randomly divided into training (244) and validation (105) sets. Least absolute shrinkage and selection operator regression was used for the selection of clinical characteristics. Six machine learning algorithms were compared: decision tree (DT), k-nearest neighbours (KNN), logistic regression model (LM), random forest (RF), support vector machine (SVM), and XGBoost (XGB). Model performance was assessed via receiver-operating characteristic (ROC), calibration, and decision curve analyses (DCAs). A nomogram was then developed on the basis of the best model. Results: Compared with the five other machine learning algorithms (DT, KNN, RF, SVM, and XGB), the LM demonstrated the highest area under the ROC curve (AUC, 0.894) and recall (0.92) in the validation set. Additionally, the calibration curves and DCA results were relatively favourable. Disease duration, DPN, insulin dosage, urinary protein, and ALB were included in the LM. The nomogram exhibited robust discrimination (AUC: 0.856 in the training set and 0.868 in the validation set), calibration, and clinical applicability across the two datasets after 1,000 bootstraps. Conclusion: Among the six different machine learning algorithms, the LM algorithm demonstrated the best performance. A logistic regression-based nomogram for predicting DR in type 2 DM patients was established. This nomogram may serve as a valuable tool for DR detection, facilitating timely treatment. [ABSTRACT FROM AUTHOR]

In coastal areas, coconuts are a common crop. Everyone from farmers to lawmakers and businesses would benefit from an accurate forecast of coconut production. Internet of Things (IoT) sensors are strategically positioned to continuously monitor the environment and gather production statistics to obtain accurate agricultural output predictions. To effectively estimate coconut prediction, this study presents an enhanced deep learning classifier called Bi-directional Long Short-Term Memory (BILSTM) with the integrated Lévy Flight and Seagull Optimization Algorithm (LFSOA). LASSO feature selection is applied to eliminate the superfluous characteristics in the yield estimation. To further enhance the coconut yield estimate, the optimal set of hyperparameters for BILSTM is tuned by the LFSOA, which helps to avoid the overfitting issue. For the results, the BILSTM is compared against different classifiers such as Recurrent Neural Network (RNN), Random Forest Classifier (RFC), and LSTM. Similarly, LFSOA-based hyperparameter tuning is contrasted with different optimization algorithms. The outputs show that LFSOA-based hyperparameter tuning in BILSTM achieved accuracy, precision, recall, and f1-score of 98.963%, 99.026%, 99.155%, and 95.758%, respectively, which are higher when compared to existing methods. Similarly, the BILSTM-LFSOA accomplished better results in statistical measures, including the Root Mean Square Error (RMSE) of 0.105, Mean Squared Error (MSE) of 0.011, Mean Absolute Error (MAE) of 0.094, and coefficient of determination (R2) of 0.954, respectively. From the overall analysis, the proposed BILSTM-LFSOA improves coconut yield prediction by achieving better results in all the performance measures when compared with existing models. The results of this study are important to many stakeholders, including but not limited to policymakers, farmers, banks, and insurance companies. As coconuts are an important crop in developing countries, accurate coconut yield forecasting will lead to greater financial and food security in these regions. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to identify prognostic signatures to predict the prognosis of patients with stomach adenocarcinoma (STAD), which is necessary to improve poor prognosis and offer possible treatment strategies for STAD patients. Methods: The overlapping genes between the key model genes that were screened by the weighted gene co-expression network analysis (WGCNA) and differentially expressed genes (DEGs) whose expression was different with significance between normal and tumor tissues were extracted to serve as co-expression genes. Then, enrichment analysis was performed on these genes. Furthermore, the least absolute shrinkage and selection operator (LASSO) regression was performed to screen the hub genes among overlapping genes. Finally, we constructed a model to explore the influence of polygenic risk scores on the survival probability of patients with STAD, and interaction effect and mediating analyses were also performed. Results: DEGs included 2,899 upregulated genes and 2,896 downregulated genes. After crossing the DEGs and light-yellow module genes that were obtained by WGCNA, a total of 39 overlapping genes were extracted. The gene enrichment analysis revealed that these genes were enriched in the prion diseases, biosynthesis of unsaturated fatty acids, RNA metabolic process, hydrolase activity, etc. PIP5K1P1, PTTG3P, and SNORD15B were determined by LASSO-Cox. The prognostic prediction of the three-gene model was established. The Cox regression analysis showed that the comprehensive risk score for three genes was an independent prognosis factor. Conclusion: PIP5K1P1, PTTG3P, and SNORD15B are related to the prognosis and overall survival of patients. The three-gene risk model constructed has independent prognosis predictive ability for STAD. [ABSTRACT FROM AUTHOR]

Spatial data is data that contains the influence of location with non-homogeneous variance at each location, or spatial heterogeneity. To address spatial heterogeneity, the Geographically Weighted Regression (GWR) model is used. However, in the GWR model, there is a phenomenon of multicollinearity, which is a strong relationship between independent variables that will reduce the accuracy of parameter estimation. To overcome multicollinearity in the GWR model, the Least Absolute Shrinkage and Selection Operator (LASSO) method is used. The LASSO method estimates the parameters of the GWR model by minimizing the sum of squared errors subject to a constraint function, which is solved using the Least Angle Regression (LARS) algorithm. This results in the Least Absolute Shrinkage and Selection Operator (LASSO) regression model to address the problem of multicollinearity in spatial data. Based on the research results, the LASSO method can overcome multicollinearity by shrinking the coefficients of parameters that contribute less and have a strong correlation with other independent variables in the GWR model, resulting in 33 final models. One of the models is for Nias Regency, where the factors influencing the poverty rate are the open unemployment rate, life expectancy, average length of schooling, gross participation rate, and per capita income. In Nias Regency, the value of s is 0.288 with an R-squared value of 0.9403. In Nias Regency, 94.03% of the variation in the poverty rate is explained by the independent variables in the model, while the remaining 5.97% is attributed to external factors not covered by the model. Coefficient of the Human Development Index variable shrinks to exactly zero, indicating that it has no effect on the poverty rate in Nias Regency. [ABSTRACT FROM AUTHOR]

Abstact: In the deregulated electricity market, it is increasingly important to accurately predict the fluctuating, nonlinear, and high‐frequent electricity price for market decision‐making. However, the uncertainties associated with electricity prices, such as non‐stationarity, nonlinearity, and high volatility, pose critical difficulties for electricity price forecasting (EPF). Unlike point forecasting, which provides only a single, deterministic estimate of future prices, probabilistic forecasting gives a more comprehensive and nuanced picture of future price dynamics, which can help market participants make better‐informed decisions when facing uncertainty. Therefore, in this paper, we propose a robust deep learning method for multi‐step probabilistic forecasting. First, we use the least absolute shrinkage and selection operator (LASSO) in the expert model to generate point forecasts. Second, we introduce the smoothly clipped absolute deviation regularization term, a nonconvex penalty with proven oracle properties in model selection, into temporal fusion transformers. Finally, we employ the proposed model to integrate point forecasts to give probabilistic forecasts. To evaluate the proposed forecasting model, real‐data experiments are conducted in the Nord Pool electricity market and the Polish Power Exchange market. Empirical results show that the proposed model has demonstrated superior probabilistic forecasting performances compared with other competitors and has proven its effectiveness in real‐world applications. [ABSTRACT FROM AUTHOR]

Objective To construct a diagnostic scoring model for early Parkinson's disease (PD) and to verify its efficacy. Methods Seventy-five patients with early PD and 75 gender-age matched healthy volunteers were selected and randomly divided into the verification group (38 patients with early PD and 37 healthy volunteers) and training group (37 patients with early PD and 38 healthy volunteers）. Medical records of subjects were collected. The least absolute shrinkage and selection operator (LASSO) was used to determine the optimal parameters through 10-fold cross-validation； the relevant diagnostic factors were screened from the relevant data of the training group, and the diagnostic scoring model was constructed according to each factor coefficient. The nomogram was constructed by Logistic regression； the receiver operating characteristic curve (ROC) was drawn, and the diagnostic efficiency and fit degree of the model were evaluated by the area under the curve (AUC) and the calibration curve. Results There was no significant difference in relevant data between the training group and the verification group (P>0. 05）. In the training group, the best parameter λ=0. 052 was determined by LASSO algorithm, and seven indexes of identification ability were screened out. The diagnosis scoring model formula =–1. 048+0. 961× RBDSQ score +0. 079× HAMA-14 score－0. 0002× NSE－0. 011×VEGF－0. 001× uric acid－0. 046× FA+0. 003× DFV. Multivariate Logistic regression analysis confirmed that the seven selected indicators could be used as independent diagnostic factors for early PD patients. In the verification group, the area under the curve of this diagnostic scoring model in diagnosing early PD patients was 0. 91, which was higher than that of seven factors alone； the fitting curve showed that the model had good goodness of fit. Conclusion Based on RBDSQ scoring, HAMA-14 scoring, VEGF, FA, NSE, uric acid and DFV, the diagnostic scoring model of early PD has been constructed, and the model has relatively high diagnostic efficiency. [ABSTRACT FROM AUTHOR]

The aim of the study is the development of methodology for accurate estimation of electric vehicle demand; which is paramount regarding various aspects of the firms decision-making such as optimal price, production level, and corresponding amounts of capital and labor; as well as supply chain, inventory control, capital financing, and operational expenses management. The forecasting methods utilized include statistical techniques (autoregressive integrated moving average [ARIMA], and polynomial regression), machine learning (nonlinear autoregressive neural network [NAR]), deep learning (long short-term memory [LSTM]), hybrid and combination forecasting. With regard to the latter method, our study experiments with four different combining model approaches, including the introduction of an original, novel combining method with the employment of a transcendental LASSO function, which is used to form combinations of forecasts generated by the NAR, ARIMA, and polynomial regression models. The LASSO -based combining model proved superior to all other models, for the majority of forecast error statistics; where the root mean square error (RMSE) and mean absolute percentage error (MAPE) values are 4.5% and 8% respectively lower than the average level of the component model forecasts. The major implications of our empirical findings are that greater accuracy in demand forecasting can be achieved with a combining model approach, rather than reliance on any particular, singular model. Furthermore, given its superior performance, the employment of the studys LASSO -based combining model to forecast electric vehicle demand may lead to optimal firm decision-making over a range of organizational facets, which is predicated on accurate demand function estimation. [ABSTRACT FROM AUTHOR]

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Abstract Patients with cytogenetically normal acute myeloid leukemia (CN-AML) may harbor prognostically relevant gene mutations and thus be categorized into one of the three 2022 European LeukemiaNet (ELN) genetic-risk groups. Nevertheless, there remains heterogeneity with respect to relapse-free survival (RFS) within these genetic-risk groups. Our training set included 306 adults on Alliance for Clinical Trials in Oncology studies with de novo CN-AML aged

Modeling ecological patterns and processes often involve large‐scale and complex high‐dimensional spatial data. Due to the nonlinearity and multicollinearity of ecological data, traditional geostatistical methods have faced great challenges in model accuracy. As machine learning has increased our ability to construct models on big data, the main focus of the study is to propose the use of statistical models that hybridize machine learning and spatial interpolation methods to cope with increasingly large‐scale and complex ecological data. Here, two machine learning algorithms, boosted regression tree (BRT) and least absolute shrinkage and selection operator (LASSO), were combined with ordinary kriging (OK) to model plant invasions across the eastern United States. The accuracies of the hybrid models and conventional models were evaluated by 10‐fold cross‐validation. Based on an invasive plants dataset of 15 ecoregions across the eastern United States, the results showed that the hybrid algorithms were significantly better at predicting plant invasion when compared to commonly used algorithms in terms of RMSE and paired‐samples t‐test (with the p‐value <.0001). Besides, the additional aspect of the combined algorithms is to have the ability to select influential variables associated with the establishment of invasive cover, which cannot be achieved by conventional geostatistics. Higher accuracy in the prediction of large‐scale biological invasions improves our understanding of the ecological conditions that lead to the establishment and spread of plants into novel habitats across spatial scales. The results demonstrate the effectiveness and robustness of the hybrid BRTOK and LASOK that can be used to analyze large‐scale and high‐dimensional spatial datasets, and it has offered an optional source of models for spatial interpolation of ecology properties. It will also provide a better basis for management decisions in early‐detection modeling of invasive species. [ABSTRACT FROM AUTHOR]

Background: Non-invasive imaging methods are still lacking for evaluating bone changes in chronic kidney diseases (CKD). Purpose: To investigate the feasibility of chest CT radiomics in evaluating bone changes caused by CKD. Material and Methods: In total, 75 patients with stage 1 CKD (CKD1) and 75 with stage 5 CKD (CKD5) were assessed using the chest CT radiomics method. Radiomics features of bone were obtained using 3D Slicer software and were then compared between CKD1 and CKD5 cases. The methods of maximum correlation minimum redundancy (mRMR) and least absolute shrinkage and selection operator (LASSO) were used to establish a prediction model to determine CKD. The receiver operating characteristic (ROC) curve was used to determine the performance of the model. Results: Cases of CKD1 and CKD5 differed in 40 radiomics features (P <0.05). Using the mRMR and LASSO methods, five features were finally selected to establish a predication model. The area under the receiver operating characteristic curve of the model in the determination of CKD1 and CKD5 was 0.903 and 0.854, respectively, for the training and validation cohorts. Conclusion: Chest CT radiomics is feasible in evaluating bone changes caused by CKD. [ABSTRACT FROM AUTHOR]

Patients with cytogenetically normal acute myeloid leukemia (CN-AML) may harbor prognostically relevant gene mutations and thus be categorized into one of the three 2022 European LeukemiaNet (ELN) genetic-risk groups. Nevertheless, there remains heterogeneity with respect to relapse-free survival (RFS) within these genetic-risk groups. Our training set included 306 adults on Alliance for Clinical Trials in Oncology studies with de novo CN-AML aged < 60 years who achieved a complete remission and for whom centrally reviewed cytogenetics, RNA-sequencing, and gene mutation data from diagnostic samples were available (Alliance trial A152010). To overcome deficiencies of the Cox proportional hazards model when long-term survivors are present, we developed a penalized semi-parametric mixture cure model (MCM) to predict RFS where RNA-sequencing data comprised the predictor space. To validate model performance, we employed an independent test set from the German Acute Myeloid Leukemia Cooperative Group (AMLCG) consisting of 40 de novo CN-AML patients aged < 60 years who achieved a complete remission and had RNA-sequencing of their pre-treatment sample. For the training set, there was a significant non-zero cure fraction (p = 0.019) with 28.5% of patients estimated to be cured. Our MCM included 112 genes associated with cure, or long-term RFS, and 87 genes associated with latency, or shorter-term time-to-relapse. The area under the curve and C-statistic were respectively, 0.947 and 0.783 for our training set and 0.837 and 0.718 for our test set. We identified a novel, prognostically relevant molecular signature in CN-AML, which allows identification of patient subgroups independent of 2022 ELN genetic-risk groups. Trial registration Data from companion studies CALGB 8461, 9665 and 20202 (trials registered at www.clinicaltrials.gov as, respectively, NCT00048958, NCT00899223, and NCT00900224) were obtained from Alliance for Clinical Trials in Oncology under data sharing study A152010. Data from the AMLCG 2008 trial was registered at www.clinicaltrials.gov as NCT01382147. [ABSTRACT FROM AUTHOR]

The increasing lifespan and large individual differences in cognitive capability highlight the importance of comprehending the aging process of the brain. Contrary to visible signs of bodily ageing, like greying of hair and loss of muscle mass, the internal changes that occur within our brains remain less apparent until they impair function. Brain age, distinct from chronological age, reflects our brain's health status and may deviate from our actual chronological age. Notably, brain age has been associated with mortality and depression. The brain is plastic and can compensate even for severe structural damage by rewiring. Functional characterization offers insights that structural cannot provide. Contrary to the multitude of studies relying on structural magnetic resonance imaging (MRI), we utilize resting-state functional MRI (rsfMRI). We also address the issue of inclusion of subjects with abnormal brain ageing through outlier removal. In this study, we employ the Least Absolute Shrinkage and Selection Operator (LASSO) to identify the 39 most predictive correlations derived from the rsfMRI data. The data is from a cohort of 176 healthy right-handed volunteers, aged 18-78 years (95/81 male/female, mean age 48, SD 17) collected at the Mind Research Imaging Center at the National Cheng Kung University. We establish a normal reference model by excluding 68 outliers, which achieves a leave-one-out mean absolute error of 2.48 years. By asking which additional features that are needed to predict the chronological age of the outliers with a smaller error, we identify correlations predictive of abnormal aging. These are associated with the Default Mode Network (DMN). Our normal reference model has the lowest prediction error among published models evaluated on adult subjects of almost all ages and is thus a candidate for screening for abnormal brain aging that has not yet manifested in cognitive decline. This study advances our ability to predict brain aging and provides insights into potential biomarkers for assessing brain age, suggesting that the role of DMN in brain aging should be studied further. [ABSTRACT FROM AUTHOR]

Airborne laser scanning technique (ALS) is the most appealing remote sensing technique for the precise estimation of forest above-ground biomass (AGB). Significantly strong correlations (collinearity) among the independent variables derived from ALS data decrease the accuracy of developed AGB models. To address this issue, we propose a novel variable selection algorithm-an improved sure independence screening (SPV), which integrates the Pearson correlation coefficient, threshold μ, and variance inflation factor. We further compared the performance of SPV-based, stepwise feature selection (SFS)-based, and least absolute shrinkage and selection operator (LASSO)-based AGB models developed with different regression approaches that were sensitive to strong collinearity. Field-measured data and corresponding ALS data, acquired from 1002 sample plots distributed across four distinct forest types within the Guangxi Zhuang Autonomous Region in Southern China, were used to evaluate variable selection techniques and develop AGB models. Results indicated that ALS variables selected by SPV exhibited weaker collinearity compared to those selected by SFS and LASSO. SPV-based AGB models outperformed SFS-based AGB models with higher leave-one-out cross-validation adjusted R2 (LOOCV Radj2) by 0.1% − 27.8%. SPV-based AGB models outperformed LASSO-based AGB models with higher LOOCV Radj2 by 0.4% − 16.3%. Hence, for variable selection in constructing AGB models (linear regression model, log–log regression model, and generalized additive model (GAM)) based on strongly collinear ALS variables, SPV is most preferred, followed by SFS and LASSO. The smooth curves from our GAMs developed using SPV-selected variables revealed that five canopy height variables (whp40, hmean, hp25, hp70, hskew), one canopy density variable (whp85), three density-related variables (nVegPts, bcc, and mcc), and one vertical structural variable (LADmean) were positively correlated with AGB. The canopy height variables (whp40, hmean, hp25, and hp70) were identified as the most important variables in estimating AGB for four forest types. The canopy density variable wdp85 showed a strong effect on AGB of the coniferous forests, whereas it had almost no effect on the AGB of broadleaved forests. Overall, this manuscript proposes a novel variable selection algorithm named SPV, aimed at addressing collinearity of variables derived from ALS data, which has significant implications for the application of ALS in forest inventory and forest modeling.

Background: Data regarding risk factors for premature coronary artery disease (PCAD) is scarce given that few research focus on it. This study aimed to develop and validate a clinical nomogram for PCAD patients in Guangzhou. Methods: We recruited 108 PCAD patients (female ≤65 years old and male ≤55 years old) and 96 healthy controls from Sun Yat-sen Memorial Hospital of Sun Yat-sen University between 01/01/2021 and 31/12/2022. Twenty potentially relevant indicators of PCAD were extracted. Next, the least absolute shrinkage and selection operator (LASSO) regression analysis was used to optimize variable selection. The nomogram was developed based on the selected variables visually. Results: Independent risk factors, including body mass index (BMI), history of PCAD, glucose, Apolipoprotein A1(ApoA1), high density lipoprotein 2-cholesterol (HDL2-C), total cholesterol and triglyceride, were identified by LASSO and logistic regression analysis. The nomogram showed accurate discrimination (area under the receiver operator characteristic curve, ROC, 87.45 %, 95 % CI: 82.58 %-92.32 %). Decision curve analysis (DCA) suggested that the nomogram was clinical beneficial. HDL2, one risk factor, was isolated by a two-step discontinuous density-gradient ultracentrifugation method. And HDL2 from PCAD patients exhibited less 3H-cholesterol efflux (22.17 % vs 26.64 %, P

Lin Feng,1 Jiachen Li,1 Zhenbei Qian,2 Chenglong Li,3,4 Darui Gao,3,4 Yongqian Wang,3,4 Wuxiang Xie,3,4 Yutong Cai,5 Zhaohui Tong,2 Lirong Liang1 1Department of Clinical Epidemiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China; 3Heart and Vascular Health Research Center, Peking University Clinical Research Institute, Peking University First Hospital, Beijing, People’s Republic of China; 4Key Laboratory of Epidemiology of Major Diseases (Peking University), Ministry of Education, Beijing, People’s Republic of China; 5Centre for Environmental Health and Sustainability, Department of Health Sciences, University of Leicester, Leicester, UKCorrespondence: Lirong Liang, Department of Clinical Epidemiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China, Email llrcruie@163.comPurpose: Patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) exhibit heterogeneous responses to corticosteroid treatment. We aimed to determine whether combining eosinophil levels with other routine clinical indicators can enhance the predictability of corticosteroid treatment outcomes and to come up with a scoring system.Patients and Methods: Consecutive patients admitted with AECOPD receiving corticosteroid treatment between July 2013 and March 2022 at Beijing Chao-Yang Hospital were retrospectively analyzed. Data on patients’ demographics, smoking status, hospitalization for AECOPD in the previous year, comorbidities, blood laboratory tests, in-hospital treatment and clinical outcomes were collected. Least absolute shrinkage and selection operator (LASSO) regression and backward logistic regression were used for predictor selection, and predictive nomograms were developed. The discrimination and calibration of the nomograms were assessed using the area under the receiver operating curve (AUC) and calibration plots. Internal validation was performed using the 500-bootstrap method, and clinical utility was evaluated using decision curve analysis (DCA).Results: Among the 3254 patients included, 804 (24.7%) had treatment failure. A nomogram of eosinophils, platelets, C-reactive protein (CRP), low density lipoprotein cholesterol, prognostic nutritional index (PNI), hospitalization for AECOPD in the previous year, ischemic heart diseases and chronic hepatic disease was developed to predict treatment failure for patients with a smoking history. For patients without a smoking history, a nomogram of CRP, PNI, ischemic heart diseases and chronic hepatic disease was developed. Although the AUCs of these two nomograms were only 0.644 and 0.647 respectively, they were significantly superior to predictions based solely on blood eosinophil levels.Conclusion: We developed easy-to-use comprehensive nomograms utilizing readily available clinical biomarkers related to inflammation, nutrition and immunity, offering modestly enhanced predictive value for treatment outcomes in corticosteroid-treated patients with AECOPD. Further investigations into novel biomarkers and additional patient data are imperative to optimize the predictive performance.Keywords: chronic obstructive pulmonary disease, glucocorticoids, prediction model, least absolute shrinkage and selection operator

Pregnancy complications in the early months of the family process can lead to miscarriage. Miscarriage does not occur due to only one reason; many factors are involved in causing miscarriage. Deep Learning (DL) can help healthcare providers by providing advanced analysis. In this study, we have proposed two novel ensemble models, Echo Dense Inception Blending (EDI-Blend) and Dense Reservoir Inception Modular Network (DRIM-Net), for miscarriage prediction. The dataset is balanced through the use of the hybrid balancing technique NearSMOTE. As the dataset is high-dimensional, we have used the Absolute Shrinkage and Selection Operator to select essential features from the dataset that significantly impact pregnancy complications. We validate the output of our proposed EDI-Blend and DRIM-Net models using 10-Fold Cross Validation. To determine the contribution of features for miscarriage prediction two eXplainable Artificial Intelligence techniques are applied to EDI-Blend and DRIM-Net: Interpretable Model-agnostic Explanations and SHapley Additive exPlanations. We have compared our proposed EDI-Blend model with base models, and the results show that EDI-Blend model performance is more efficient, with 0.732 accuracy, 0.721 recall, 0.732 F1-score, and 0.721 Receiver Operating Characteristic-Area Under the Curve (ROC-AUC). The DRIM-Net model is also compared with baseline models and achieves 0.768 F1-score, 0.764 precision, 0.769 accuracy, 0.769 recall, and 0.837 ROC-AUC.

Background: Epidermal growth factor receptor (EGFR) and Kirsten rat sarcoma viral oncogene homolog (KRAS) are mutually exclusive, and they are two important genes that are most prone to mutation in patients with non‐small cell lung cancer. Purpose: This retrospective study investigated the ability of radiomics to predict the mutation status of EGFR and KRAS in patients with non‐small cell lung cancer (NSCLC) and guide precision medicine. Methods: Computed tomography images of 1045 NSCLC patients from five different institutions were collected, and 1204 imaging features were extracted. In the training set (EGFR: 678, KRAS: 246), Max‐Relevance and Min‐Redundancy and least absolute shrinkage and selection operator logistic regression were used to screen radiomics features. The combination of selected radiomics features and clinical factors was used to establish the combined models in identifying EGFR and KRAS mutation status, respectively, through stepwise logistic regression. Then, on two independent external validation sets (EGFR: 203/164, KRAS: 123/95), the performance of each model was evaluated separately, and then the overall performance of predicting the two mutation states was calculated. Results: In the EGFR and KRAS groups, radiomics signatures comprised 14 and 10 radiomics features, respectively. They were mutually exclusive between the tumors with positive EGFR mutation and those with positive KRAS mutation in imaging phenotype. For the EGFR group, the area under the curve (AUC) of the combined model in the two validation sets was 0.871 (95% CI: 0.821–0.926) and 0.861 (95% CI: 0.802–0.911), respectively, whereas the AUC of the combined model in the two validation sets was 0.798 (95% CI: 0.739–0.850) and 0.778 (95% CI: 0.735–0.821), respectively, for the KRAS group. Considering both EGFR and KRAS, the overall precision, recall, and F1‐score of the combined model in the two validation sets were 0.704, 0.844, and 0.768, as well as 0.754, 0.693, and 0.722, respectively. Conclusions: Our study demonstrates the potential of radiomics in the non‐invasive identification of EGFR and KRAS mutation status, which may guide patients with non‐small cell lung cancer to choose the most appropriate personalized treatment. This method can be used when biopsy will bring unacceptable risk to patients with NSCLC. [ABSTRACT FROM AUTHOR]

The improved Sparse Signal Reconstruction (SR) algorithm for Trusted Artificial Intelligence (AI) and Distributed Compressed Sensing (DCS) technology was thoroughly investigated. The study verified its effectiveness and advantages in trusted AI and DCS systems, which have significant implications for enhancing the credibility, security, and performance of signal processing and AI algorithms. The reconstruction performance was evaluated using Orthogonal Matching Pursuit (OMP), Basis Pursuit (BP), and Least Absolute Shrinkage and Selection Operator (LASSO). The analysis primarily focused on runtime, refactoring errors, and the number of successful reconstruction attempts. When K = 4, K = 6, K = 8, and K = 10, OMP outperformed BP and LASSO in terms of successful reconstructions, demonstrating better performance and higher reconstruction precision. [ABSTRACT FROM AUTHOR]

Abstract Modeling ecological patterns and processes often involve large‐scale and complex high‐dimensional spatial data. Due to the nonlinearity and multicollinearity of ecological data, traditional geostatistical methods have faced great challenges in model accuracy. As machine learning has increased our ability to construct models on big data, the main focus of the study is to propose the use of statistical models that hybridize machine learning and spatial interpolation methods to cope with increasingly large‐scale and complex ecological data. Here, two machine learning algorithms, boosted regression tree (BRT) and least absolute shrinkage and selection operator (LASSO), were combined with ordinary kriging (OK) to model plant invasions across the eastern United States. The accuracies of the hybrid models and conventional models were evaluated by 10‐fold cross‐validation. Based on an invasive plants dataset of 15 ecoregions across the eastern United States, the results showed that the hybrid algorithms were significantly better at predicting plant invasion when compared to commonly used algorithms in terms of RMSE and paired‐samples t‐test (with the p‐value

Background Few prediction models for individuals with early‐stage out‐of‐hospital cardiac arrest (OHCA) have undergone external validation. This study aimed to externally validate updated prediction models for OHCA outcomes using a large nationwide dataset. Methods and Results We performed a secondary analysis of the JAAM‐OHCA (Comprehensive Registry of In‐Hospital Intensive Care for Out‐of‐Hospital Cardiac Arrest Survival and the Japanese Association for Acute Medicine Out‐of‐Hospital Cardiac Arrest) registry. Previously developed prediction models for patients with cardiac arrest who achieved the return of spontaneous circulation were updated. External validation was conducted using data from 56 institutions from the JAAM–OHCA registry. The primary outcome was a dichotomized 90‐day cerebral performance category score. Two models were updated using the derivation set (n=3337). Model 1 included patient demographics, prehospital information, and the initial rhythm upon hospital admission; Model 2 included information obtained in the hospital immediately after the return of spontaneous circulation. In the validation set (n=4250), Models 1 and 2 exhibited a C‐statistic of 0.945 (95% CI, 0.935–0.955) and 0.958 (95% CI, 0.951–0.960), respectively. Both models were well‐calibrated to the observed outcomes. The decision curve analysis showed that Model 2 demonstrated higher net benefits at all risk thresholds than Model 1. A web‐based calculator was developed to estimate the probability of poor outcomes (https://pcas‐prediction.shinyapps.io/90d_lasso/). Conclusions The updated models offer valuable information to medical professionals in the prediction of long‐term neurological outcomes for patients with OHCA, potentially playing a vital role in clinical decision‐making processes.

ObjectiveTo investigate the relationships between perfluoroalkyl and polyfluoroalkyl substances (PFASs) exposure and glucose metabolism indices.MethodsData from the National Health and Nutrition Examination Survey (NHANES) 2017–2018 waves were used. A total of 611 participants with information on serum PFASs (perfluorononanoic acid (PFNA); perfluorooctanoic acid (PFOA); perfluoroundecanoic acid (PFUA); perfluorohexane sulfonic acid (PFHxS); perfluorooctane sulfonates acid (PFOS); perfluorodecanoic acid (PFDeA)), glucose metabolism indices (fasting plasma glucose (FPG), homeostasis model assessment for insulin resistance (HOMA-IR) and insulin) as well as selected covariates were included. We used cluster analysis to categorize the participants into three exposure subgroups and compared glucose metabolism index levels between the subgroups. Least absolute shrinkage and selection operator (LASSO), multiple linear regression analysis and Bayesian kernel machine regression (BKMR) were used to assess the effects of single and mixed PFASs exposures and glucose metabolism.ResultsThe cluster analysis results revealed overlapping exposure types among people with higher PFASs exposure. As the level of PFAS exposure increased, FPG level showed an upward linear trend (p 50 years-old group: β = 0.059, p 50 years-old group: insulin β = 0.191, p = 0.018, HOMA-IR β = 0.220, p = 0.013) showed positive correlation with HOMA-IR/insulin, which was essentially the same as results that obtained for the univariate exposure-response map in the BKMR model. Association of exposure to PFASs on glucose metabolism indices showed positive interactions between PFOS and PFHxS and negative interactions between PFOA and PFNA/PFOS/PFHxS.ConclusionOur study provides evidence that positive and negative correlations between PFASs and FPG and HOMA-IR/insulin levels are observed, respectively. Combined effects and interactions between PFASs. Given the higher risk of glucose metabolism associated with elevated levels of PFAS, future studies are needed to explore the potential underlying mechanisms.

In coastal areas, coconuts are a common crop. Everyone from farmers to lawmakers and businesses would benefit from an accurate forecast of coconut production. Internet of Things (IoT) sensors are strategically positioned to continuously monitor the environment and gather production statistics to obtain accurate agricultural output predictions. To effectively estimate coconut prediction, this study presents an enhanced deep learning classifier called Bi-directional Long Short-Term Memory (BILSTM) with the integrated Lévy Flight and Seagull Optimization Algorithm (LFSOA). LASSO feature selection is applied to eliminate the superfluous characteristics in the yield estimation. To further enhance the coconut yield estimate, the optimal set of hyperparameters for BILSTM is tuned by the LFSOA, which helps to avoid the overfitting issue. For the results, the BILSTM is compared against different classifiers such as Recurrent Neural Network (RNN), Random Forest Classifier (RFC), and LSTM. Similarly, LFSOA-based hyperparameter tuning is contrasted with different optimization algorithms. The outputs show that LFSOA-based hyperparameter tuning in BILSTM achieved accuracy, precision, recall, and f1-score of 98.963%, 99.026%, 99.155%, and 95.758%, respectively, which are higher when compared to existing methods. Similarly, the BILSTM-LFSOA accomplished better results in statistical measures, including the Root Mean Square Error (RMSE) of 0.105, Mean Squared Error (MSE) of 0.011, Mean Absolute Error (MAE) of 0.094, and coefficient of determination (R2) of 0.954, respectively. From the overall analysis, the proposed BILSTM-LFSOA improves coconut yield prediction by achieving better results in all the performance measures when compared with existing models. The results of this study are important to many stakeholders, including but not limited to policymakers, farmers, banks, and insurance companies. As coconuts are an important crop in developing countries, accurate coconut yield forecasting will lead to greater financial and food security in these regions.

Objective: The purpose of this study was to identify potential biomarkers in the tubulointerstitium of focal segmental glomerulosclerosis (FSGS) and comprehensively analyze its mRNA-miRNA-lncRNA/circRNA network. Methods: The expression data (GSE108112 and GSE200818) were downloaded from the Gene Expression Omnibus database (https://www.ncbi.nlm.nih.gov/geo/). Identification and enrichment analysis of differentially expressed genes (DEGs) were performed. the PPI networks of the DEGs were constructed and classified using the Cytoscape molecular complex detection (MCODE) plugin. Weighted gene coexpression network analysis (WGCNA) was used to identify critical gene modules. Least absolute shrinkage and selection operator regression analysis were used to screen for key biomarkers of the tubulointerstitium in FSGS, and the receiver operating characteristic curve was used to determine their diagnostic accuracy. The screening results were verified by quantitative real-time-PCR (qRT-PCR) and Western blot. The transcription factors (TFs) affecting the hub genes were identified by Cytoscape iRegulon. The mRNA-miRNA-lncRNA/circRNA network for identifying potential biomarkers was based on the starBase database. Results: A total of 535 DEGs were identified. MCODE obtained eight modules. The green module of WGCNA had the greatest association with the tubulointerstitium in FSGS. PPARG coactivator 1 alpha (PPARGC1A) was screened as a potential tubulointerstitial biomarker for FSGS and verified by qRT-PCR and Western blot. The TFs FOXO4 and FOXO1 had a regulatory effect on PPARGC1A. The ceRNA network yielded 17 miRNAs, 32 lncRNAs, and 50 circRNAs. Conclusions: PPARGC1A may be a potential biomarker in the tubulointerstitium of FSGS. The ceRNA network contributes to the comprehensive elucidation of the mechanisms of tubulointerstitial lesions in FSGS. [ABSTRACT FROM AUTHOR]

The aim of our study was to identify key biomarkers of glomeruli in focal glomerulosclerosis (FSGS) and analyze their relationship with the infiltration of immune cells. The expression profiles (GSE108109 and GSE200828) were obtained from the GEO database. The differentially expressed genes (DEGs) were filtered and analyzed by gene set enrichment analysis (GSEA). MCODE module was constructed. Weighted gene coexpression network analysis (WGCNA) was performed to obtain the core gene modules. Least absolute shrinkage and selection operator (LASSO) regression was applied to identify key genes. ROC curves were employed to explore their diagnostic accuracy. Transcription factor prediction of the key biomarkers was performed using the Cytoscape plugin IRegulon. The analysis of the infiltration of 28 immune cells and their correlation with the key biomarkers were performed. A total of 1474 DEGs were identified. Their functions were mostly related to immune-related diseases and signaling pathways. MCODE identified five modules. The turquoise module of WGCNA had significant relevance to the glomerulus in FSGS. TGFB1 and NOTCH1 were identified as potential key glomerular biomarkers in FSGS. Eighteen transcription factors were obtained from the two hub genes. Immune infiltration showed significant correlations with T cells. The results of immune cell infiltration and their relationship with key biomarkers implied that NOTCH1 and TGFB1 were enhanced in immune-related pathways. TGFB1 and NOTCH1 may be strongly correlated with the pathogenesis of the glomerulus in FSGS and are new candidate key biomarkers. T-cell infiltration plays an essential role in the FSGS lesion process. [ABSTRACT FROM AUTHOR]

Objectives: Triple-negative breast cancer (TNBC) poses a significant challenge due to the lack of reliable prognostic gene signatures and an understanding of its immune behavior. Methods: We analyzed clinical information and mRNA expression data from 162 TNBC patients in TCGA-BRCA and 320 patients in METABRIC-BRCA. Utilizing weighted gene coexpression network analysis, we pinpointed 34 TNBC immune genes linked to survival. The least absolute shrinkage and selection operator Cox regression method identified key TNBC immune candidates for prognosis prediction. We calculated chemotherapy sensitivity scores using the "pRRophetic" package in R software and assessed immunotherapy response using the Tumor Immune Dysfunction and Exclusion algorithm. Results: In this study, 34 survival-related TNBC immune gene expression profiles were identified. A least absolute shrinkage and selection operator-Cox regression model was used and 15 candidates were prioritized, with a concomitant establishment of a robust risk immune classifier. The high-risk TNBC immune groups showed increased sensitivity to therapeutic agents like RO-3306, Tamoxifen, Sunitinib, JNK Inhibitor VIII, XMD11-85h, BX-912, and Tivozanib. An analysis of the Search Tool for Interaction of Chemicals database revealed the associations between the high-risk group and signaling pathways, such as those involving Rap1, Ras, and PI3K-Akt. The low-risk group showed a higher immunotherapy response rate, as observed through the tumor immune dysfunction and exclusion analysis in the TCGA-TNBC and METABRIC-TNBC cohorts. Conclusion: This study provides insights into the immune complexities of TNBC, paving the way for novel diagnostic approaches and precision treatment methods that exploit its immunological intricacies, thus offering hope for improved management and outcomes of this challenging disease. [ABSTRACT FROM AUTHOR]

In this article, we address the problem of lung cancer diagnosis from gene expression data, which is now recognized as an effective means for early treatment and prevention of cancer. Specifically, we employ Fuzzy min–max (FMM) classifier for the task which is a well‐known neuro‐fuzzy neural network. The idea is to take advantage of the fuzzy class definitions whose boundaries are set using the min–max hyperbox constructed for each class. We implement two advanced FMM neural network architectures: general Fuzzy min–max (GFMM) and enhanced Fuzzy min–max (EFMM) for the classification of lung cancer subtypes from gene expression data. The advantage of GFMM is that it involves very simple operations for hyperbox manipulation, and can handle both labeled and unlabeled data. On the other hand, EFMM proposes three heuristic rules related to hyperbox expansion, contraction and the overlap test, which enhances the learning algorithm. We perform the classification of gene expression data using these two models, and then we analyze the performance by visualizing the hyperboxes obtained after training, and compare the accuracies of these classifiers with the state of the art. Least absolute shrinkage and selection operator (LASSO) is used for selecting the informative genes from the high‐dimensional gene expression data. From the empirical results, we observe that GFMM with LASSO gives the best performance of all, with validation accuracy of 98.04% and cross‐validation accuracy of 94.06%. [ABSTRACT FROM AUTHOR]

Objective: Tongue squamous cell carcinoma (TSCC) is one of the most common and poor prognosis head and neck tumors. The purpose of this study is to establish a model for predicting TSCC prognosis based on clinical and MR radiomics data and to develop a nomogram. Methods: A retrospective analysis was performed on the clinical and imaging data of 211 patients with pathologically confirmed TSCC who underwent radical surgery at xx hospital from February 2011 to January 2020. Patients were divided into a study group (recurrence, metastasis, and death, n = 76) and a control group (normal survival, n = 135) according to 1 to 6 years of follow-up. A training set and a test set were established based on a ratio of 7:3 and a time point. In the training set, 3 prediction models (clinical data model, imaging model, and combined model) were established based on the MR radiomics score (Radscore) combined with clinical features. The predictive performance of these models was compared using the Delong curve, and the clinical net benefit of the model was tested using the decision curve. Then, the external validation of the model was performed in the test set, and a nomogram for predicting TSCC prognosis was developed. Results: Univariate analysis confirmed that betel nut consumption, spicy hot pot or pickled food, unclean oral sex, drug use, platelet/lymphocyte ratio (PLR), neutrophil/lymphocyte ratio (NLR), depth of invasion (DOI), low differentiation, clinical stage, and Radscore were factors that affected TSCC prognosis (P <.05). In the test set, the combined model based on these factors had the highest predictive performance for TSCC prognosis (area under curve (AUC) AUC: 0.870, 95% CI [0.761-0.942]), which was significantly higher than the clinical model (AUC: 0.730, 95% CI [0.602-0.835], P =.033) and imaging model (AUC: 0.765, 95% CI [0.640-0.863], P =.074). The decision curve also confirmed the higher clinical net benefit of the combined model, and these results were validated in the test set. The nomogram developed based on the combined model received good evaluation in clinical application. Conclusion: MR-LASSO extracted texture parameters can help improve the performance of TSCC prognosis models. The combined model and nomogram provide support for postoperative clinical treatment management of TSCC. [ABSTRACT FROM AUTHOR]

Ciguatera poisoning is a globally occurring seafood disease caused by the ingestion of marine products contaminated with dinoflagellate produced neurotoxins. Persistent forms of ciguatera, which prove to be highly debilitating, are poorly studied and represent a significant medical issue. The present study aims to better understand chronic ciguatera manifestations and identify potential predictive factors for their duration. Medical files of 49 patients were analyzed, and the post-hospitalization evolution of the disease assessed through a follow-up questionnaire. A rigorous logistic lasso regression model was applied to select significant predictors from a list of 37 patient characteristics potentially predictive of having chronic symptoms. Missing data were handled by complete case analysis, and a survival analysis was implemented. All models used standardized variables, and multiple comparisons in the survival analyses were handled by Bonferroni correction. Among all studied variables, five significant predictors of having symptoms lasting ≥3 months were identified: age, tobacco consumption, acute bradycardia, laboratory measures of urea, and neutrophils. This exploratory, hypothesis-generating study contributes to the development of ciguatera epidemiology by narrowing the list from 37 possible predictors to a list of five predictors that seem worth further investigation as candidate risk factors in more targeted studies of ciguatera symptom duration.

AbstractObjective The aim of our study was to identify key biomarkers of glomeruli in focal glomerulosclerosis (FSGS) and analyze their relationship with the infiltration of immune cells.Methods The expression profiles (GSE108109 and GSE200828) were obtained from the GEO database. The differentially expressed genes (DEGs) were filtered and analyzed by gene set enrichment analysis (GSEA). MCODE module was constructed. Weighted gene coexpression network analysis (WGCNA) was performed to obtain the core gene modules. Least absolute shrinkage and selection operator (LASSO) regression was applied to identify key genes. ROC curves were employed to explore their diagnostic accuracy. Transcription factor prediction of the key biomarkers was performed using the Cytoscape plugin IRegulon. The analysis of the infiltration of 28 immune cells and their correlation with the key biomarkers were performed.Results A total of 1474 DEGs were identified. Their functions were mostly related to immune-related diseases and signaling pathways. MCODE identified five modules. The turquoise module of WGCNA had significant relevance to the glomerulus in FSGS. TGFB1 and NOTCH1 were identified as potential key glomerular biomarkers in FSGS. Eighteen transcription factors were obtained from the two hub genes. Immune infiltration showed significant correlations with T cells. The results of immune cell infiltration and their relationship with key biomarkers implied that NOTCH1 and TGFB1 were enhanced in immune-related pathways.Conclusion TGFB1 and NOTCH1 may be strongly correlated with the pathogenesis of the glomerulus in FSGS and are new candidate key biomarkers. T-cell infiltration plays an essential role in the FSGS lesion process.

Abstract In this article, we address the problem of lung cancer diagnosis from gene expression data, which is now recognized as an effective means for early treatment and prevention of cancer. Specifically, we employ Fuzzy min–max (FMM) classifier for the task which is a well‐known neuro‐fuzzy neural network. The idea is to take advantage of the fuzzy class definitions whose boundaries are set using the min–max hyperbox constructed for each class. We implement two advanced FMM neural network architectures: general Fuzzy min–max (GFMM) and enhanced Fuzzy min–max (EFMM) for the classification of lung cancer subtypes from gene expression data. The advantage of GFMM is that it involves very simple operations for hyperbox manipulation, and can handle both labeled and unlabeled data. On the other hand, EFMM proposes three heuristic rules related to hyperbox expansion, contraction and the overlap test, which enhances the learning algorithm. We perform the classification of gene expression data using these two models, and then we analyze the performance by visualizing the hyperboxes obtained after training, and compare the accuracies of these classifiers with the state of the art. Least absolute shrinkage and selection operator (LASSO) is used for selecting the informative genes from the high‐dimensional gene expression data. From the empirical results, we observe that GFMM with LASSO gives the best performance of all, with validation accuracy of 98.04% and cross‐validation accuracy of 94.06%.

Groundnut is a key oilseed crop in the world and India is one of the largest groundnuts producing country in terms of area and yield. Keeping that in view, five models were developed for five districts of Andhra Pradesh to forecast the groundnut yield viz., Stepwise Multiple Linear Regression (SMLR), Ridge regression, Least Absolute Shrinkage and Selection Operator (LASSO), Elastic Net (ELNET) and Artificial Neural Network (ANN). The historical data on the weather parameters are obtained from NASA POWER web portal and groundnut yields for these districts of the state during both Kharif and Rabi seasons obtained through Season and Crop Report, Government of Andhra Pradesh for the period, 2001 to 2020. In total 30 weather indices were generated through five weather variables. The assessment of models was done by fixing 75 % of the data for calibration and left 25 % data for validation. The findings inferred that based on the values of R², RMSE, nRMSE and EF, Ridge regression, ELNET and ANN models showed better performance for Ananthapur, Chittoor and Kadapa districts and SMLR and LASSO models showed better performance for Kurnool and Nellore districts during both Kharif and Rabi seasons at calibration and validation stages. [ABSTRACT FROM AUTHOR]

Objective: People with diabetes have a higher risk of suicidal behaviors than the general population. However, few studies have focused on understanding this relationship. We investigated risk factors and predicted suicide attempts in people with diabetes using Least Absolute Shrinkage and Selection Operator (LASSO) regression. Method: Data was retrieved from Cerner Real-World Data and included over 3 million diabetes patients in the study. LASSO regression was applied to identify associated factors. Gender, diabetes-type, and depression-specific LASSO regression models were analyzed. Results: There were 7764 subjects diagnosed with suicide attempts with an average age of 45. Risk factors for suicide attempts in diabetes patients were American Indian or Alaska Native race (β = 0.637), receiving atypical antipsychotic agents (β = 0.704), benzodiazepines (β = 0.784), or antihistamines (β = 0.528). Amyotrophy was negatively associated with suicide attempts in males (β = 2.025); in contrast, amyotrophy significantly increased the risk in females (β = 3.339). Using a MAOI was negatively related to suicide attempts in T1DM patients (β = 7.304). Age less than 20 was positively associated with suicide attempts in depressed (β = 2.093) and non-depressed patients (β = 1.497). The LASSO model achieved a 94.4% AUC and 87.4% F1 score. Conclusions: To our knowledge, this is the first study to use LASSO regression to identify risk factors for suicide attempts in patients with diabetes. The shrinkage technique successfully reduced the number of variables in the model to improve the fit. Further research is needed to determine cause-and-effect relationships. The results may help providers to identify high-risk groups for suicide attempt among diabetic patients. [ABSTRACT FROM AUTHOR]

Abstract Background Utilizing population-based survey data in epidemiological research with a spatial perspective can integrate valuable context into the dynamics of HIV prevalence in West Africa. However, the situation in the Mano River Union (MRU) countries is largely unknown. This research aims to perform an ecological study to determine the HIV prevalence patterns in MRU. Methods We analyzed Demographic and Health Survey (DHS) and AIDS Indicator Survey (AIS) data on HIV prevalence in MRU from 2005 to 2020. We examined the country-specific, regional-specific and sex-specific ratios of respondents to profile the spatial–temporal heterogeneity of HIV prevalence and determine HIV hot spots. We employed Geodetector to measure the spatial stratified heterogeneity (SSH) of HIV prevalence for adult women and men. We assessed the comprehensive correct knowledge (CCK) about HIV/AIDS and HIV testing uptake by employing the Least Absolute Shrinkage and Selection Operator (LASSO) regression to predict which combinations of CCKs can scale up the ratio of HIV testing uptake with sex-specific needs. Results In our analysis, we leveraged data for 158,408 respondents from 11 surveys in the MRU. From 2005–2015, Cote d'Ivoire was the hot spot for HIV prevalence with a Gi_Bin score of 3, Z-Score 8.0–10.1 and P

Objective: Studies have shown that cancer progression of head and neck squamous cell carcinoma (HNSCC) is related with metabolic alterations. The aim of this study is to identify the clinical roles of metabolic alterations in HNSCC. Materials and methods: Metabolism‐related genes associated with HNSCC were searched in public databases. A predictive and efficacious LASSO model was fabricated to optimize the diagnosis that was based on these genes. Meantime, ultra‐performance liquid chromatography‐quadrupole/orbitrap high resolution mass spectrometry (UHPLC‐Q‐Orbitrap HRMS) was used to compare patients with HNSCC (n = 73) with healthy controls (n = 51) for serum metabolites. Potential biomarkers and alterations in serum metabolites were analysed and evaluated using t test analysis, principal component analysis and orthogonal partial least square‐discrimination analysis. Results: Overall, 21 differential metabolites were probed in serum, of which eight metabolites had potential for clinical uses. Transcriptome analysis showed that four genes in the constructed LASSO model were found to be associated with seven differential metabolites. Metabolic pathway analysis by MetaboAnalyst showed that the biomarkers that were related with HNSCC were closely related to four metabolism pathways (p < 0.05). Conclusion: To conclude, future research on HNSCC should be directed towards multi‐omics to provide treatment, intervention or diagnosis of the disease. [ABSTRACT FROM AUTHOR]

Groundnut is a key oilseed crop in the world and India is one of the largest groundnuts producing country in terms of area and yield. Keeping that in view, five models were developed for five districts of Andhra Pradesh to forecast the groundnut yield viz., Stepwise Multiple Linear Regression (SMLR), Ridge regression, Least Absolute Shrinkage and Selection Operator (LASSO), Elastic Net (ELNET) and Artificial Neural Network (ANN). The historical data on the weather parameters are obtained from NASA POWER web portal and groundnut yields for these districts of the state during both Kharif and Rabi seasons obtained through Season and Crop Report, Government of Andhra Pradesh for the period, 2001 to 2020. In total 30 weather indices were generated through five weather variables. The assessment of models was done by fixing 75 % of the data for calibration and left 25 % data for validation. The findings inferred that based on the values of R2, RMSE, nRMSE and EF, Ridge regression, ELNET and ANN models showed better performance for Ananthapur, Chittoor and Kadapa districts and SMLR and LASSO models showed better performance for Kurnool and Nellore districts during both Kharif and Rabi seasons at calibration and validation stages.

Kai Wang, Yian Tian, Shanshan Liu, Zhongyuan Zhang, Leilei Shen, Deqian Meng, Ju Li Department of Rheumatology, the Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huaian, 223001, People’s Republic of ChinaCorrespondence: Ju Li; Deqian Meng, Department of Rheumatology, the affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huaian, 223001, People’s Republic of China, Email liju198461@163.com; deqianmeng2016njmc@163.comBackground: Rapidly progressive interstitial lung disease (RP-ILD) is a significant complication that determines the prognosis of dermatomyositis (DM). Early RP-ILD diagnosis can improve screening and diagnostic efficiency and provide meaningful guidance to carry out early and aggressive treatment.Methods: A retrospective screening of 284 patients with DM was performed. Clinical and laboratory characteristics of the patients were recorded. The risk factors of RP-ILD in DM patients were screened by logistic regression (LR) and machine learning methods, and the prediction models of RP-ILD were developed by machine learning methods, namely least absolute shrinkage and selection operator (LASSO), random forest (RF), and extreme gradient boosting (XGBoost).Results: According to the result of univariate LR, disease duration is a protective factor for RP-ILD, and ESR, CRP, anti-Ro-52 antibody and anti-MDA5 antibody are risk factors for RP-ILD. The top 10 important variables of the 3 machine learning models were intersected to obtain common important variables, and there were 5 common important variables, namely disease duration, LDH, CRP, anti-Ro-52 antibody and anti-MDA5 antibody. The AUC of LASSO, RF and XGBoost test set were 0.661, 0.667 and 0.867, respectively. We further validated the performance of these three models on validation set, and the results showed that, the AUC of LASSO, RF and XGBoost were 0.764, 0.727 and 0.909, respectively. Based on the results of the models, XGBoost is the optimal model in this study.Conclusion: Disease duration, LDH, CRP, anti-Ro-52 antibody and anti-MDA5 antibody are vital risk factors for RP-ILD in DM. The prediction model constructed using XGBoost can be used for risk identification and early intervention in DM patients with RP-ILD and practical application.Keywords: dermatomyositis, interstitial lung disease, risk factor, predictive model, logistic regression, least absolute shrinkage and selection operator, random forest, extreme gradient boosting

The effective early prediction of clinical outcomes of Parkinson's disease (PD) is of great significance in the implementation of appropriate interventions. We aimed to propose a method based on the use of baseline resting‐state functional characteristics (i.e., fractional amplitude of low‐frequency fluctuations, fALFF) to predict motor progression in PD patients. Resting‐state functional magnetic resonance imaging was performed on 48 newly‐diagnosed drug‐naïve PD patients and 27 age‐ and sex‐ matched healthy controls (HCs). Two PD subgroups were defined with different annual increase of Unified PD Rating Scale Part III motor scores. Least absolute shrinkage and selection operator regression analysis was performed to explore the baseline region‐functional indicators for PD discrimination as well as the predictors for future motor deficits. Two significant models composed of baseline fALFF values from cerebral subregions were proposed. The classification model that distinguished PD patients from HCs (area under the curve [AUC] = 0.897) showed the most significant imaging characteristics in the putamen and precentral gyrus. The other prediction model that evaluated the degree of future deterioration of motor symptoms in PD patients (AUC = 0.916) showed the most significant imaging characteristics in the superior occipital gyrus and caudate nucleus. Furthermore, the increased regional function in bilateral caudate nuclei was correlated with the lower annual increase in motor deficits in all PD patients. The caudate nucleus might be the core region responsible for future motor deficits in newly‐diagnosed PD patients, which may aid the development of disease progression preventive strategies in clinical practice. [ABSTRACT FROM AUTHOR]