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27 results on '"Leblond, Marine M."'

2. Development of Traceable Mouse Models of Advanced and Metastatic Bladder Cancer.

Author

Desponds, Emma, Kioseoglou, Konstantina, Zdimerova, Hana, Ongaro, Marco, Verdeil, Grégory, and Leblond, Marine M.

Subjects
BLADDER tumors, BIOLOGICAL models, TRANSGENIC animals, RESEARCH funding, IMMUNOTHERAPY, ANIMALS, IN vivo studies, MANN Whitney U Test, METASTASIS, GENE expression, MICE, IMMUNOHISTOCHEMISTRY, CELL lines, IMMUNE checkpoint inhibitors, ANIMAL experimentation, ONE-way analysis of variance, GENETIC mutation, TUMOR antigens, DISEASE progression
Abstract

Simple Summary: Poor prognosis for advanced bladder cancer underlines the need for a better understanding of tumor resistance to treatment in relevant preclinical models. We developed new models of inducible and orthotopic muscle-invasive bladder cancer, with strong phenotypical and genetic resemblances to the human disease. These models enable real-time tracking of tumor progression and metastasis through bioluminescence imaging to facilitates the testing of new therapies. This valuable tool is important for advancing bladder cancer research and developing more effective treatments. Bladder cancer (BC) is the fourth most common cancer in men, with a poor patient prognosis for advanced disease. The poor survival of patients with muscle-invasive bladder cancer (MIBC) and metastatic status emphasizes the urgent need to develop new therapies. Lacking in the field of BC is the availability of relevant advanced BC mouse models, especially metastatic ones, that accurately recapitulate the complexities of human pathology to test and study new therapeutic strategies. Addressing this need, we developed a traceable mouse model of BC that expresses tumor-associated antigens within the context of advanced muscle-invasive BC. This novel system was achieved through the deletion of the tp53 and pten genes, alongside the incorporation of the fusion construct of Firefly luciferase (Luc) and the SIYRYYGL (SIY) T-cell antigen. We validate that the presence of the transgene did not impact on the development of the tumors while allowing us to measure tumor progression by bioluminescence. We show that the transgene did not influence the composition of the immune tumor microenvironment. More importantly, we report that this model was unresponsive to anti-PD-1 treatment, as in the majority of patients with BC. We also develop a new model based on the orthotopic injection of BC clonal cell lines derived from our first model. We demonstrate that this new model invades the muscle layer and has a metastasis development rate of 83%. The advantage of this model is that we can visualize tumor growth and metastasis development in vivo. These mouse models' characteristics, displaying many similarities with the human pathology, provide a valuable tool for tracking tumor progression, metastasis spread in vivo, and treatment resistance, as well as exploring fundamental and translational aspects of BC biology. This work contributes to the improvement in the landscape of mouse models of advanced BC for testing new therapeutic strategies. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Immuno-Transcriptomic Profiling of Blood and Tumor Tissue Identifies Gene Signatures Associated with Immunotherapy Response in Metastatic Bladder Cancer

Author

Desponds, Emma, primary, Croci, Davide, additional, Wosika, Victoria, additional, Hadadi, Noushin, additional, Fonseca Costa, Sara S., additional, Ciarloni, Laura, additional, Ongaro, Marco, additional, Zdimerova, Hana, additional, Leblond, Marine M., additional, Hosseinian Ehrensberger, Sahar, additional, Romero, Pedro, additional, and Verdeil, Grégory, additional

Published
2024
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8. Investigating the effects of proton therapy versus X-rays on circulating leukocytes in a context of brain irradiation: a preclinical study

Author

Coupey, Julie, Pham, Thao Nguyen, Toutain, Jérôme, Hue, Erika, Hélaine, Charly, Richard, Jade, Saulnier, Romaric, Chazalviel, Laurent, Rousseau, Marc, Thariat, Juliette, Leblond, Marine M., Valable, Samuel, Imagerie et Stratégies Thérapeutiques pour les Cancers et Tissus cérébraux (ISTCT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO), Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN), Normandie Université (NU)-Normandie Université (NU), Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales (ISTCT), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Unité Support CYCERON, Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physique corpusculaire de Caen (LPCC), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Radiothérapie [Centre François Baclesse], Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), and Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

CERVOXY; National audience

Published
2022

9. Comparative study of proton versus X-rays on circulating leukocytes in a context of brain irradiation: a preclinical study

Author

Coupey, Julie, Pham, Thao Nguyen, Toutain, Jérôme, Hue, Erika, Hélaine, Charly, Richard, Jade, Saulnier, Romaric, Chazalviel, Laurent, Rousseau, Marc, Thariat, Juliette, Leblond, Marine M., Valable, Samuel, Imagerie et Stratégies Thérapeutiques pour les Cancers et Tissus cérébraux (ISTCT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO), Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN), Normandie Université (NU)-Normandie Université (NU), Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales (ISTCT), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Unité Support CYCERON, Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physique corpusculaire de Caen (LPCC), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Radiothérapie [Centre François Baclesse], Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), and Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

CERVOXY; National audience

Published
2022

10. Activation of the transcription factor NFAT5 in the tumor microenvironment enforces CD8+T cell exhaustion

Author

Tillé, Laure, Cropp, Daniela, Charmoy, Mélanie, Reichenbach, Patrick, Andreatta, Massimo, Wyss, Tania, Bodley, Gabrielle, Crespo, Isaac, Nassiri, Sina, Lourenco, Joao, Leblond, Marine M., Lopez-Rodriguez, Cristina, Speiser, Daniel E., Coukos, George, Irving, Melita, Carmona, Santiago J., Held, Werner, and Verdeil, Grégory

Abstract

Persistent exposure to antigen during chronic infection or cancer renders T cells dysfunctional. The molecular mechanisms regulating this state of exhaustion are thought to be common in infection and cancer, despite obvious differences in their microenvironments. Here we found that NFAT5, an NFAT family transcription factor that lacks an AP-1 docking site, was highly expressed in exhausted CD8+T cells in the context of chronic infections and tumors but was selectively required in tumor-induced CD8+T cell exhaustion. Overexpression of NFAT5 in CD8+T cells reduced tumor control, while deletion of NFAT5 improved tumor control by promoting the accumulation of tumor-specific CD8+T cells that had reduced expression of the exhaustion-associated proteins TOX and PD-1 and produced more cytokines, such as IFNɣ and TNF, than cells with wild-type levels of NFAT5, specifically in the precursor exhausted PD–1+TCF1+TIM–3–CD8+T cell population. NFAT5 did not promote T cell exhaustion during chronic infection with clone 13 of lymphocytic choriomeningitis virus. Expression of NFAT5 was induced by TCR triggering, but its transcriptional activity was specific to the tumor microenvironment and required hyperosmolarity. Thus, NFAT5 promoted the exhaustion of CD8+T cells in a tumor-selective fashion.

Published
2023
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12. c‐Maf enforces cytokine production and promotes memory‐like responses in mouse and human type 2 innate lymphoid cells

Author

Trabanelli, Sara, primary, Ercolano, Giuseppe, additional, Wyss, Tania, additional, Gomez‐Cadena, Alejandra, additional, Falquet, Maryline, additional, Cropp, Daniela, additional, Imbratta, Claire, additional, Leblond, Marine M, additional, Salvestrini, Valentina, additional, Curti, Antonio, additional, Adotevi, Olivier, additional, Jandus, Camilla, additional, and Verdeil, Grégory, additional

Published
2022
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14. Helicobacter pylori infection has a detrimental impact on the efficacy of cancer immunotherapies

Author

Oster, Paul, primary, Vaillant, Laurie, additional, Riva, Erika, additional, McMillan, Brynn, additional, Begka, Christina, additional, Truntzer, Caroline, additional, Richard, Corentin, additional, Leblond, Marine M, additional, Messaoudene, Meriem, additional, Machremi, Elisavet, additional, Limagne, Emeric, additional, Ghiringhelli, Francois, additional, Routy, Bertrand, additional, Verdeil, Gregory, additional, and Velin, Dominique, additional

Published
2021
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16. Helicobacter pylori infection has a detrimental impact on the efficacy of cancer immunotherapies.

Author

Oster, Paul, Vaillant, Laurie, Riva, Erika, McMillan, Brynn, Begka, Christina, Truntzer, Caroline, Richard, Corentin, Leblond, Marine M, Messaoudene, Meriem, Machremi, Elisavet, Limagne, Emeric, Ghiringhelli, Francois, Routy, Bertrand, Verdeil, Gregory, and Velin, Dominique

Subjects
HELICOBACTER pylori infections, BACTEROIDES fragilis, DERMATOPHAGOIDES pteronyssinus, BACTERIAL colonies, TUMOR-infiltrating immune cells
Published
2022
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19. Helicobacter pyloriinfection has a detrimental impact on the efficacy of cancer immunotherapies

Author

Oster, Paul, Vaillant, Laurie, Riva, Erika, McMillan, Brynn, Begka, Christina, Truntzer, Caroline, Richard, Corentin, Leblond, Marine M, Messaoudene, Meriem, Machremi, Elisavet, Limagne, Emeric, Ghiringhelli, Francois, Routy, Bertrand, Verdeil, Gregory, and Velin, Dominique

Abstract

ObjectiveIn this study, we determined whether Helicobacter pylori(H. pylori) infection dampens the efficacy of cancer immunotherapies.DesignUsing mouse models, we evaluated whether immune checkpoint inhibitors or vaccine-based immunotherapies are effective in reducing tumour volumes of H. pylori-infected mice. In humans, we evaluated the correlation between H. pyloriseropositivity and the efficacy of the programmed cell death protein 1 (PD-1) blockade therapy in patients with non-small-cell lung cancer (NSCLC).ResultsIn mice engrafted with MC38 colon adenocarcinoma or B16-OVA melanoma cells, the tumour volumes of non-infected mice undergoing anticytotoxic T-lymphocyte-associated protein 4 and/or programmed death ligand 1 or anti-cancer vaccine treatments were significantly smaller than those of infected mice. We observed a decreased number and activation status of tumour-specific CD8+T cells in the tumours of infected mice treated with cancer immunotherapies independent of the gut microbiome composition. Additionally, by performing an in vitro co-culture assay, we observed that dendritic cells of infected mice promote lower tumour-specific CD8+T cell proliferation. We performed retrospective human clinical studies in two independent cohorts. In the Dijon cohort, H. pyloriseropositivity was found to be associated with a decreased NSCLC patient survival on anti-PD-1 therapy. The survival median for H. pyloriseropositive patients was 6.7 months compared with 15.4 months for seronegative patients (p=0.001). Additionally, in the Montreal cohort, H. pyloriseropositivity was found to be associated with an apparent decrease of NSCLC patient progression-free survival on anti-PD-1 therapy.ConclusionOur study unveils for the first time that the stomach microbiota affects the response to cancer immunotherapies and that H. pyloriserology would be a powerful tool to personalize cancer immunotherapy treatment.

Published
2022
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20. Hyperoxic gas-induced changes in two models of glioma: an MRI study to assess blood volume and oxygen saturation in rats

Author

Chakhoyan, Ararat, Corroyer-Dulmont, Aurelien, Leblond, Marine M., Gerault, Aurelie, Toutain, Jerome, Chazaviel, Laurent, Divoux, Didier, Petit, Edwige, Mackenzie, Eric T., Kauffaann, Francois, Delcroix, Nicolas, Myriam Bernaudin, Touzani, Omar, Valable, Samuel, Hypoxie, physiopathologies cérébrovasculaire et tumorale (CERVOxy), Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales (ISTCT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Mathématiques Nicolas Oresme (LMNO), Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Unité Support CYCERON, Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Centre National de la Recherche Scientifique (CNRS), and Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

CERVOXY; International audience

Published
2016

22. Hypoxia induces macrophage polarization and re-education towards an M2 phenotype in glioblastoma

Author

Leblond, Marine M., Gerault, Aurelie N., Corroyer-Dulmont, Aurelien, Petit, Edwige, Myriam Bernaudin, Valable, Samuel, Brunaud, Carole, Hypoxie, physiopathologies cérébrovasculaire et tumorale (CERVOxy), Imagerie et Stratégies Thérapeutiques des pathologies Cérébrales et Tumorales (ISTCT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

CERVOXY; International audience

Published
2015

23. Carbogen-induced increases in tumor oxygenation depend on the vascular status of the tumor: A multiparametric MRI study in two rat glioblastoma models

Author

Chakhoyan, Ararat, primary, Corroyer-Dulmont, Aurélien, additional, Leblond, Marine M, additional, Gérault, Aurélie, additional, Toutain, Jérôme, additional, Chazaviel, Laurent, additional, Divoux, Didier, additional, Petit, Edwige, additional, MacKenzie, Eric T, additional, Kauffmann, François, additional, Delcroix, Nicolas, additional, Bernaudin, Myriam, additional, Touzani, Omar, additional, and Valable, Samuel, additional

Published
2016
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26. Hypoxia induces macrophage polarization and re-education toward an M2 phenotype in U87 and U251 glioblastoma models.

Author

Leblond, Marine M, Gérault, Aurélie N, Corroyer-Dulmont, Aurélien, MacKenzie, Eric T, Petit, Edwige, Bernaudin, Myriam, and Valable, Samuel

Subjects
*HYPOXIA (Water), *MACROPHAGES, *PHENOTYPES, *BRAIN tumors, *GLIOBLASTOMA multiforme, *PROGNOSIS
Abstract

Hypoxia is a common feature of solid tumors, particularly in glioblastoma (GBM), and known to be a poor prognosis factor in GBM patients. The growth of GBM is also associated with a marked inflammation partially characterized by an accumulation of macrophage (MΦ) of the M2 phenotype. However, the transition between M1 MΦ (antitumoral) and M2 MΦ (protumoral) phenotypes is a dynamic process. We made the assumption that oxygen (O2) availability could be a major regulator of this transition and that the intratumoral O2gradient is of importance. We evaluated,in vivo, the impact of hypoxia on MΦ tropism and polarization in two models of human GBM, well differentiated by their degree of hypoxia. MΦ migration in the tumor was more pronounced in the more hypoxic tumor of the two GBM models. In the more hypoxic of the models, we have shown that MΦ migrated at the tumor site only when hypoxia takes place. We also demonstrated that the acquisition of the M2 phenotype was clearly an evolving phenomenon with hypoxia as the major trigger for this transition. In support of thesein vivofinding, M0 but also M1 MΦ cultured in moderate or severe hypoxia displayed a phenotype close to that of M2 MΦ whose phenotype was further reinforced by severe hypoxia. These results highlight the role of hypoxia in the aggressiveness of GBM, in part, by transforming MΦ such that a protumoral activity is expressed. [ABSTRACT FROM PUBLISHER]

Published
2016
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27. Flow cytometry detection and quantification of circulating leukocyte subpopulations in mice after brain irradiation.

Author

Coupey J, Leblond MM, Hue ES, and Valable S

Subjects
Animals, Mice, Leukocytes radiation effects, Tumor Microenvironment immunology, Glioblastoma radiotherapy, Glioblastoma pathology, Glioblastoma immunology, Glioblastoma blood, Lymphopenia etiology, Lymphopenia pathology, Lymphopenia blood, Flow Cytometry methods, Brain pathology, Brain radiation effects, Brain Neoplasms radiotherapy, Brain Neoplasms pathology
Abstract

In the context of high-grade gliomas such as glioblastoma (GBM), the immune part of the tumor microenvironment (TME) is involved in tumor growth and tumor recurrence. It is mostly represented by high amount of macrophages and low amount of lymphocytes. GBM in itself as well as x-ray-based radiotherapy, a standard treatment for brain tumors, are also associated with systemic effects like lymphopenia that correlates with a poor prognosis. This contributes to the immune-suppressive nature of the TME and may explain the lack of the anti-tumor immune response. Radiation-induced lymphopenia (RIL) is generally evaluated on CD4 + and CD8 + count or on a CBC (complete blood count), but the heterogeneity of the subtypes prompts us to explore them in detail to better understand the cellular response to brain irradiation. To facilitate and develop the evaluation of x-ray brain exposure on circulating immune cells, we developed a reproducible and reliable method to quantify the variation of lymphoid and myeloid subtypes using flow cytometry after brain irradiation in the rodent., Competing Interests: Conflict of interest statement The authors declare no conflict of interest., (Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.)

Published
2024
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