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113 results on '"Lederis, Karl"'

9. Mutational analysis and molecular modeling of the nonapeptide hormone binding domains of the [Arg8] vasotocin receptor

Author

Hausmann, Holger, Richters, Anke, Kreienkamp, Hans-Jurgen, Meyerhof, Wolfgang, Mattes, Henri, Lederis, Karl, Zwiers, Henk, and Richter, Dietmar

Subjects
Osteichthyes -- Genetic aspects, Binding sites (Biochemistry) -- Research, Ligand binding (Biochemistry) -- Research, Science and technology
Abstract

To identify determinants that form nonapeptide hormone binding domains of the white sucker Catostomus commersoni [[Arg.sup.8]]vasotocin receptor, chimeric constructs encoding parts of the vasotocin receptor and parts of the isotocin receptor have been analyzed by [(3,[5.sup.-3]H)[Tyr.sup.2], [Arg.sup.8]]vasotocin binding to membranes of human embryonic kidney cells previously transfected with the different cDNA constructs and by functional expression studies in Xenopus laevis oocytes injected with mutant cRNAs. The results indicate that the N terminus and a region spanning the second extracellular loop and its flanking transmembrane segments, which contains a number of amino acid residues that are conserved throughout the nonapeptide receptor family, contribute to the affinity of the receptor for its ligand. Nonapeptide selectivity, however, is mainly defined by transmembrane region VI and the third extracellular loop. These results are complemented by a molecular model of the vasotocin receptor obtained by aligning its sequence with those of other G-protein coupled receptors as well as that of bacteriorhodopsin. The model indicates that amino acid residues of transmembrane regions II-VII that are located close to the extracellular surface also contribute to the binding of vasotocin.

Published
1996

13. Structure, function, and phylogeny of (Arg8)vasotocin receptors from teleost fish and toad

Author

Mahlmann, Stefan, Meyerhof, Wolfgang, Hausmann, Holger, Heierhorst, Jorg, Schonrock, Christiane, Zwiers, Henk, Lederis, Karl, and Richter, Dietmar

Subjects
Oxytocin -- Research, Phylogeny -- Research, Fishes -- Genetic aspects, Science and technology
Abstract

An (Arg8)vasotocin (AVT) receptor containing 435 amino acid residues has been cloned from the teleost fish, the white sucker, to analyze the molecular evaluation of associated receptor family of the vasopressin oxytocin hormone family. The receptor is associated with an ancestral nonapeptide receptor. Metabolic, sensory and osmoregulation mechanisms probably involve a physiological function for this receptor.

Published
1994

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