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27 results on '"Lee, Fang-Hua"'

1. Predictors of Nonseroconversion after SARS-CoV-2 Infection

Author

Liu, Weimin, Russell, Ronnie M., Bibollet-Ruche, Frederic, Skelly, Ashwin N., Sherrill-Mix, Scott, Freeman, Drew A., Stoltz, Regina, Lindemuth, Emily, Lee, Fang-Hua, Sterrett, Sarah, Bar, Katharine J., Erdmann, Nathaniel, Gouma, Sigrid, Hensley, Scott E., Ketas, Thomas, Cupo, Albert, Portillo, Victor M. Cruz, Moore, John P., Bieniasz, Paul D., Hatziioannou, Theodora, Massey, Greer, Minyard, Mary-Beth, Saag, Michael S., Davis, Randall S., Shaw, George M., Britt, William J., Leal, Sixto M., Jr., Goepfert, Paul, and Hahn, Beatrice H.

Subjects
Development and progression, Research, Patient outcomes, Antigen-antibody reactions -- Research, Immunologic research, COVID-19 -- Development and progression -- Patient outcomes, Immunological research
Abstract

Coronavirus disease (COVID-19) is typically diagnosed by reverse transcription PCR (RT-PCR) amplification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA from nasopharyngeal fluids (1). RT-PCR yields cycle threshold ([C.sub.t]) [...]

Published
2021
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2. Envelope residue 375 substitutions in simian–human immunodeficiency viruses enhance CD4 binding and replication in rhesus macaques

Author

Li, Hui, Wang, Shuyi, Kong, Rui, Ding, Wenge, Lee, Fang-Hua, Parker, Zahra, Kim, Eunlim, Learn, Gerald H., Hahn, Paul, Policicchio, Ben, Brocca-Cofano, Egidio, Deleage, Claire, Hao, Xingpei, Chuang, Gwo-Yu, Gorman, Jason, Gardner, Matthew, Lewis, Mark G., Hatziioannou, Theodora, Santra, Sampa, Apetrei, Cristian, Pandrea, Ivona, Alam, S. Munir, Liao, Hua-Xin, Shen, Xiaoying, Tomaras, Georgia D., Farzan, Michael, Chertova, Elena, Keele, Brandon F., Estes, Jacob D., Lifson, Jeffrey D., Doms, Robert W., Montefiori, David C., Haynes, Barton F., Sodroski, Joseph G., Kwong, Peter D., Hahn, Beatrice H., and Shaw, George M.

Published
2016

5. New SHIVs and Improved Design Strategy for Modeling HIV-1 Transmission, Immunopathogenesis, Prevention, and Cure

Author

Li, Hui, primary, Wang, Shuyi, additional, Lee, Fang-Hua, additional, Roark, Ryan S., additional, Murphy, Alex I., additional, Smith, Jessica, additional, Zhao, Chengyan, additional, Rando, Juliette, additional, Chohan, Neha, additional, Ding, Yu, additional, Kim, Eunlim, additional, Lindemuth, Emily, additional, Bar, Katharine J., additional, Pandrea, Ivona, additional, Apetrei, Cristian, additional, Keele, Brandon F., additional, Lifson, Jeffrey D., additional, Lewis, Mark G., additional, Denny, Thomas N., additional, Haynes, Barton F., additional, Hahn, Beatrice H., additional, and Shaw, George M., additional

Published
2021
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7. Recapitulation of HIV-1 Env-antibody coevolution in macaques leading to neutralization breadth

Author

Roark, Ryan S., primary, Li, Hui, additional, Williams, Wilton B., additional, Chug, Hema, additional, Mason, Rosemarie D., additional, Gorman, Jason, additional, Wang, Shuyi, additional, Lee, Fang-Hua, additional, Rando, Juliette, additional, Bonsignori, Mattia, additional, Hwang, Kwan-Ki, additional, Saunders, Kevin O., additional, Wiehe, Kevin, additional, Moody, M. Anthony, additional, Hraber, Peter T., additional, Wagh, Kshitij, additional, Giorgi, Elena E., additional, Russell, Ronnie M., additional, Bibollet-Ruche, Frederic, additional, Liu, Weimin, additional, Connell, Jesse, additional, Smith, Andrew G., additional, DeVoto, Julia, additional, Murphy, Alexander I., additional, Smith, Jessica, additional, Ding, Wenge, additional, Zhao, Chengyan, additional, Chohan, Neha, additional, Okumura, Maho, additional, Rosario, Christina, additional, Ding, Yu, additional, Lindemuth, Emily, additional, Bauer, Anya M., additional, Bar, Katharine J., additional, Ambrozak, David, additional, Chao, Cara W., additional, Chuang, Gwo-Yu, additional, Geng, Hui, additional, Lin, Bob C., additional, Louder, Mark K., additional, Nguyen, Richard, additional, Zhang, Baoshan, additional, Lewis, Mark G., additional, Raymond, Donald D., additional, Doria-Rose, Nicole A., additional, Schramm, Chaim A., additional, Douek, Daniel C., additional, Roederer, Mario, additional, Kepler, Thomas B., additional, Kelsoe, Garnett, additional, Mascola, John R., additional, Kwong, Peter D., additional, Korber, Bette T., additional, Harrison, Stephen C., additional, Haynes, Barton F., additional, Hahn, Beatrice H., additional, and Shaw, George M., additional

Published
2021
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8. Recapitulation of HIV-1 Env-Antibody Coevolution in Macaques Leading to Neutralization Breadth

Author

Roark, Ryan S., primary, Li, Hui, additional, Williams, Wilton B., additional, Chug, Hema, additional, Mason, Rosemarie D., additional, Gorman, Jason, additional, Wang, Shuyi, additional, Lee, Fang-Hua, additional, Rando, Juliette, additional, Bonsignori, Mattia, additional, Hwang, Kwan-Ki, additional, Saunders, Kevin O., additional, Wiehe, Kevin, additional, Moody, M. Anthony, additional, Hraber, Peter T., additional, Wagh, Kshitij, additional, Giorgi, Elena E., additional, Russell, Ronnie M., additional, Bibollet-Ruche, Frederic, additional, Liu, Weimin, additional, Connell, Jesse, additional, Smith, Andrew G., additional, DeVoto, Julia, additional, Murphy, Alexander I., additional, Smith, Jessica, additional, Ding, Wenge, additional, Zhao, Chengyan, additional, Chohan, Neha, additional, Okumura, Maho, additional, Rosario, Christina, additional, Ding, Yu, additional, Lindemuth, Emily, additional, Bauer, Anya M., additional, Bar, Katharine J., additional, Ambrozak, David, additional, Chao, Cara W., additional, Chuang, Gwo-Yu, additional, Geng, Hui, additional, Lin, Bob C., additional, Louder, Mark K., additional, Nguyen, Richard, additional, Zhang, Baoshan, additional, Lewis, Mark G., additional, Raymond, Donald, additional, Doria-Rose, Nicole A., additional, Schramm, Chaim A., additional, Douek, Daniel C., additional, Roederer, Mario, additional, Kepler, Thomas B., additional, Kelsoe, Garnett, additional, Mascola, John R., additional, Kwong, Peter D., additional, Korber, Bette T., additional, Harrison, Stephen C., additional, Haynes, Barton F., additional, Hahn, Beatrice H., additional, and Shaw, George M., additional

Published
2020
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13. The Major Cellular Sterol Regulatory Pathway Is Required for Andes Virus Infection

Author

Petersen, Josiah, primary, Drake, Mary Jane, additional, Bruce, Emily A., additional, Riblett, Amber M., additional, Didigu, Chukwuka A., additional, Wilen, Craig B., additional, Malani, Nirav, additional, Male, Frances, additional, Lee, Fang-Hua, additional, Bushman, Frederic D., additional, Cherry, Sara, additional, Doms, Robert W., additional, Bates, Paul, additional, and Briley, Kenneth, additional

Published
2014
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14. Transmitted/Founder and Chronic HIV-1 Envelope Proteins Are Distinguished by Differential Utilization of CCR5

Author

Parker, Zahra F., primary, Iyer, Shilpa S., additional, Wilen, Craig B., additional, Parrish, Nicholas F., additional, Chikere, Kelechi C., additional, Lee, Fang-Hua, additional, Didigu, Chuka A., additional, Berro, Reem, additional, Klasse, Per Johan, additional, Lee, Benhur, additional, Moore, John P., additional, Shaw, George M., additional, Hahn, Beatrice H., additional, and Doms, Robert W., additional

Published
2013
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16. Adaptive Mutations in a Human Immunodeficiency Virus Type 1 Envelope Protein with a Truncated V3 Loop Restore Function by Improving Interactions with CD4

Author

Agrawal-Gamse, Caroline, primary, Lee, Fang-Hua, additional, Haggarty, Beth, additional, Jordan, Andrea P. O., additional, Yi, Yanjie, additional, Lee, Benhur, additional, Collman, Ronald G., additional, Hoxie, James A., additional, Doms, Robert W., additional, and Laakso, Meg M., additional

Published
2009
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18. An Engineered Saccharomyces cerevisiae Strain Binds the Broadly Neutralizing Human Immunodeficiency Virus Type 1 Antibody 2G12 and Elicits Mannose-Specific gp120-Binding Antibodies

Author

Luallen, Robert J., primary, Lin, Jianqiao, additional, Fu, Hu, additional, Cai, Karen K., additional, Agrawal, Caroline, additional, Mboudjeka, Innocent, additional, Lee, Fang-Hua, additional, Montefiori, David, additional, Smith, David F., additional, Doms, Robert W., additional, and Geng, Yu, additional

Published
2008
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20. V3 Loop Truncations in HIV-1 Envelope Impart Resistance to Coreceptor Inhibitors and Enhanced Sensitivity to Neutralizing Antibodies

Author

Laakso, Meg M, primary, Lee, Fang-Hua, additional, Haggarty, Beth, additional, Agrawal, Caroline, additional, Nolan, Katrina M, additional, Biscone, Mark, additional, Romano, Josephine, additional, Jordan, Andrea P. O, additional, Leslie, George J, additional, Meissner, Eric G, additional, Su, Lishan, additional, Hoxie, James A, additional, and Doms, Robert W, additional

Published
2007
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21. Dissecting the Neutralizing Antibody Specificities of Broadly Neutralizing Sera from Human Immunodeficiency Virus Type 1-Infected Donors

Author

Dhillon, Amandeep K., primary, Donners, Helen, additional, Pantophlet, Ralph, additional, Johnson, Welkin E., additional, Decker, Julie M., additional, Shaw, George M., additional, Lee, Fang-Hua, additional, Richman, Douglas D., additional, Doms, Robert W., additional, Vanham, Guido, additional, and Burton, Dennis R., additional

Published
2007
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25. The role of cytochromes c in the electron transport chain of Haemophilus parainfluenzae

Author

Lee, Fang-Hua and Lee, Fang-Hua

Abstract

The electron transport system of Haemophilus parainfluenzae, which can use nitrate or fumarate or oxygen as terminal electron acceptors, has been examined with emphasis on the functions of cytochromes c. Differential synthesis of electron transport components was observed in cells under varying growth conditions. When H. parainfluenzae are grown anaerobically with nitrate a significant increase in the concentration of c-type cytochromes and decrease in the cytochrome d are found. Polarographic assays indicate the existence of NADH-, succinate-, formate-, TMPD- and quinol- oxidases in membrane fractions. A novel NADH-bovine cytochrome c reductase which is resistant to cytochrome $bc\sb1$ inhibitors is found in membrane particles in spectrophotometric assays. Cytochromes $b\sb{558}$, $b\sb{598}$ and d which are responsible for quinol oxidase activity are located exclusively in the membrane fraction. We have identified four cytochromes c with molecular weights of 57.5, 33.6, 23.8, and 14 kDa in H. parainfluenzae. A soluble cytochrome c-57.5 kDa, which can be reoxidized with nitrite but not nitrate, was extracted under all growth conditions tested. It was isolated from the soluble fraction of early stationary phase organisms grown anaerobically with nitrate when the cytochrome is present in high concentrations. This cytochrome $c\sb{552}$-57.5 kDa was purified to electrophoretic homogeneity by ion-exchange, hydroxylapatite and gel filtration chromatography. Reduced minus oxidized difference spectra show $\alpha$- and $\beta$-bands at 552 nm and 522 nm and a Soret band at 421 nm. The cytochrome $c\sb{552}$ has a low redox potential, is not reducible by ascorbate, binds CO when reduced by dithionite and is autooxidizable. The pI determined by isoelectric focusing gel electrophoresis is 5.8. The purified cytochrome $c\sb{552}$ shows nitrite reductase activity which reduces nitrite to ammonia, as assayed with methyl viologen as the electron donor. Pyridine hemochromogen ana

Published
1990

26. Receptor-Ligand Binding in the Cell-Substrate Contact Zone: A Quantitative Analysis Using CX3CR1 and CXCR1 Chemokine Receptors.

Author

Lee, Fang-Hua, Haskell, Christopher, Charo, Isreal F., and David Boettiger

Subjects
*LIGANDS (Biochemistry), *THERMODYNAMICS, *QUANTITATIVE chemical analysis, *METHODOLOGY, *CARRIER proteins, *CELL receptors
Abstract

Receptor-ligand binding analyses have generally used soluble components to measure thermodynamic binding constants. In their biological context, adhesion receptors bind to an immobile ligand and the binding reaction is confined to the cell-substrate contact zone. We have developed a new procedure based on the spinning disk technology to measure the number of receptor -- ligand bonds in the contact zone. Application of this methodology to the CX3CR1 --fractalkine and the CXCR1 -IL-8 receptor- ligand systems demonstrated that the level of binding to an immobilized ligand is reduced by several orders of magnitude in comparison to Solution binding. A comparison of the Solution binding and contact zone binding constants shows that the effect of ligand immobilization was similar for each system. In contrast, although the CXCR1 -- IL-8 bond had the higher affinity, the average bond strength was only 10% of that for the CX3CR1 bond. Because fractalkine can be expressed as a cell surface-bound protein, CX3CR1 has been Proposed to function as an adhesion receptor. The higher bond strength suggests that the bond architecture has also evolved to serve an adhesion function. [ABSTRACT FROM AUTHOR]

Published
2004
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27. V3 Loop Truncations in HIV-1 Envelope Impart Resistance to Coreceptor Inhibitors and Enhanced Sensitivity to Neutralizing Antibodies

Author

Leslie, George J., Jordan, Andrea P. O., Doms, Robert W., Biscone, Mark, Agrawal, Caroline, Romano, Josephine, Su, Lishan, Haggarty, Beth, Hoxie, James A., Meissner, Eric G., Laakso, Meg M., Lee, Fang-Hua, and Nolan, Katrina M.

Subjects
viruses, virus diseases, 3. Good health
Abstract

The V1/V2 region and the V3 loop of the human immunodeficiency virus type I (HIV-1) envelope (Env) protein are targets for neutralizing antibodies and also play an important functional role, with the V3 loop largely determining whether a virus uses CCR5 (R5), CXCR4 (X4), or either coreceptor (R5X4) to infect cells. While the sequence of V3 is variable, its length is highly conserved. Structural studies indicate that V3 length may be important for interactions with the extracellular loops of the coreceptor. Consistent with this view, genetic truncation of the V3 loop is typically associated with loss of Env function. We removed approximately one-half of the V3 loop from three different HIV-1 strains, and found that only the Env protein from the R5X4 strain R3A retained some fusion activity. Loss of V1/V2 (ΔV1/V2) was well tolerated by this virus. Passaging of virus with the truncated V3 loop resulted in the derivation of a virus strain that replicated with wild-type kinetics. This virus, termed TA1, retained the V3 loop truncation and acquired several adaptive changes in gp120 and gp41. TA1 could use CCR5 but not CXCR4 to infect cells, and was extremely sensitive to neutralization by HIV-1 positive human sera, and by antibodies to the CD4 binding site and to CD4-induced epitopes in the bridging sheet region of gp120. In addition, TA1 was completely resistant to CCR5 inhibitors, and was more dependent upon the N-terminal domain of CCR5, a region of the receptor that is thought to contact the bridging sheet of gp120 and the base of the V3 loop, and whose conformation may not be greatly affected by CCR5 inhibitors. These studies suggest that the V3 loop protects HIV from neutralization by antibodies prevalent in infected humans, that CCR5 inhibitors likely act by disrupting interactions between the V3 loop and the coreceptor, and that altered use of CCR5 by HIV-1 associated with increased sensitivity to changes in the N-terminal domain can be linked to high levels of resistance to these antiviral compounds.

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