Search

Your search keyword '"Lee, Jaeyoon"' showing total 153 results
Start Over Author "Lee, Jaeyoon"
153 results on '"Lee, Jaeyoon"'

9. Chromatinization Modulates Topoisomerase II Processivity

Author

Lee, Jaeyoon, primary, Wu, Meiling, additional, Inman, James T., additional, Singh, Gundeep, additional, Park, Seong ha, additional, Lee, Joyce H., additional, Fulbright, Robert M., additional, Hong, Yifeng, additional, Jeong, Joshua, additional, Berger, James M., additional, and Wang, Michelle D., additional

Published
2023
Full Text
View/download PDF

12. Targeting the NANOG/HDAC1 axis reverses resistance to PD-1 blockade by reinvigorating the antitumor immunity cycle

Author

Oh, Se Jin, Lee, Hyo-Jung, Song, Kwon-Ho, Kim, Suyeon, Cho, Eunho, Lee, Jaeyoon, Bosenberg, Marcus W., and Kim, Tae Woo

Subjects
Tumors -- Genetic aspects -- Drug therapy, Hydrolases -- Health aspects -- Physiological aspects -- Genetic aspects, Drug resistance -- Genetic aspects, Immunity -- Genetic aspects, Membrane proteins -- Health aspects -- Physiological aspects -- Genetic aspects, Enzymes -- Health aspects -- Physiological aspects -- Genetic aspects, Transcription factors -- Physiological aspects -- Health aspects, Health care industry
Abstract

Immune checkpoint blockade (ICB) therapy has shifted the paradigm for cancer treatment. However, the majority of patients lack effective responses because of the emergence of immune-refractory tumors that disrupt the amplification of antitumor immunity. Therefore, the identification of clinically available targets that restrict antitumor immunity is required to develop potential combination therapies. Here, using transcriptomic data on patients with cancer treated with programmed cell death protein 1 (PD-1) therapy and newly established mouse preclinical anti-PD-1 therapy-refractory models, we identified NANOG as a factor restricting the amplification of the antitumor immunity cycle, thereby contributing to the immune-refractory feature of the tumor microenvironment (TME). Mechanistically, NANOG induced insufficient T cell infiltration and resistance to CTL-mediated killing via the histone deacetylase 1-dependent (HDAC1-dependent) regulation of CXCL10 and MCL1, respectively. Importantly, HDAC1 inhibition using an actionable agent sensitized [NANOG.sup.hi] immune-refractory tumors to PD-1 blockade by reinvigorating the antitumor immunity cycle. Thus, our findings implicate the NANOG/HDAC1 axis as a central molecular target for controlling immune-refractory tumors and provide a rationale for combining HDAC inhibitors to reverse the refractoriness of tumors to ICB therapy., Introduction Immune checkpoint blockade (ICB) therapy elicits a marked clinical response in patients with various tumor types, changing the paradigm for cancer treatment (1-3). However, despite the developing field's potential [...]

Published
2022
Full Text
View/download PDF

47. 누구와 창업할 것인가? 스타트업 공동창업팀 내 심층적 다양성의 필요영역

Author

Lee, JaeYoon, Oh, SooJin, and Kim, MyungUn

Abstract

스타트업이라는 새로운 형태의 조직이 급속히 확산하고 있다. 스타트업이 ‘신생의 불리함’을 극복하고 성장하기 위해서는 초기 공동창업팀을 잘 구성해야 한다. 스타트업 창업팀과 초기경영진을 연구했던 국내외 57개 문헌을 개관한 결과, 창업팀의 ‘다양성’에 대한 연구가 활발히 이루어졌다는 점을 확인할 수 있었다. 하지만 기존 문헌들은 성별, 연령, 전문분야, 창업 전 경험과 같은 ‘표면적 다양성’을 주로 연구했다. 이에 반해, 창업팀원들의 가치, 성격, 사고방식과 같은 ‘심층적 다양성’을 다룬 연구는 거의 없었다. 본 연구는 스타트업 창업팀 내에서 어떤 영역의 심층적 다양성이 필요한지에 대해 살펴보았다. 이 주제에 대한 국내외 연구의 일천함을 고려하여, 현장 창업자들의 직접 경험을 바탕으로 한 질적 연구를 실시했으며(연구 1), 여기서 도출된 결과를 정량적으로 검증해 보았다(연구 2). 이를 위해 연구 1에서는 19명의 창업자 및 관련자를 대상으로 인터뷰를 실시한 결과, 창업팀에서 필요한 심층적 다양성 영역으로 4개의 개인 특성 요인들(외향성, 의견 수용, 위험 감수, 낙관주의)과 4개의 업무 관련 요인(업무 관련 의견, 속도 중시, 큰 그림 중시, 시간관점)이 파악 되었다. 연구 2에서는 이들 8개 심층적 다양성 영역을 총 30명의 스타트업 관련자를 대상으로 설문조사를 실시해 정량적으로 검증해보았다. 이 결과, 낙관주의와 속도 중시를 제외한 나머지 6개 영역에서 다양성의 중요성이 확인되었고 낙관주의와 속도 중시는 경향성만이 확인 되었다., Start-ups organizations are increasing rapidly. To overcome “liability of newness,” it is essential for entrepreneurs to compose the right co-founding team. Based on the review of 57 journal articles on start-up co-founding teams and new venture teams, we found that the composition of the team, especially diversity among team members, was frequently studied. However, previous research has only focused on surface-level diversity such as gender, age, functional background, previous experience. Only a very few studies investigated deep-level diversity such as co-founding team members’ value, personality or thinking style. The present study explored what type of diversity is required in start-ups. Since this topic is rarely studied, we first conducted a qualitative study by interviewing with nineteen start-up founders and venture capitalists in Study 1. As a result we found that four individual characteristic factors (extraversion, agreeing to different idea, risk taking, optimism) and four work-related factors (business opinion, speed oriented, big-picture oriented, time perspective) were the key component of deep-level diversity. In Study 2, we conducted a quantitative study to empirically investigate these aspects by a survey to thirty start-up related individuals. The result confirmed that the diversity were required for six aspects deducted from Study 1. For the other factors of optimism and speed orientation, frequency tendency supported Study 1’s result.

Published
2017
Full Text
View/download PDF

49. Ratiometric Fluorescence Sensing System for Lead Ions Based on Self-Assembly of Bioprobes Triggered by Specific Pb2+–Peptide Interactions

Author

Mehta, Pramod Kumar, Lee, JaeYoon, Oh, Eun-Taex, Park, Heon Joo, and Lee, Keun-Hyeung

Abstract

Lead is one of the most toxic substances. However, there are few ratiometric fluorescent probes for sensing Pb2+in aqueous solution as well as living cells because specific ligands for Pb2+ions have not been well characterized. Considering the interactions between Pb2+and peptides, we developed ratiometric fluorescent probes for Pb2+based on the peptide receptor in two steps. First, we synthesized fluorescent probes (1–3) based on the tetrapeptide receptor (ECEE-NH2) containing hard and soft ligands by conjugation with diverse fluorophores that showed excimer emission when they aggregated. After investigation of fluorescent responses to metal ions, benzothiazolyl-cyanovinylene was evaluated as an appropriate fluorophore for ratiometric detection of Pb2+. Next, we modified the peptide receptor to decrease the number of hard ligands and/or to replace Cys with disulfide bond and methylated Cys for improving selectivity and cell permeability. From this process, we developed two fluorescent probes (3and 8) among the probes (1–8) that exhibited remarkable ratiometric sensing properties for Pb2+including high water solubility (≤2% DMF), visible light excitation, high sensitivity, selectivity for Pb2+, low detection limits (<10 nM), and fast response (<6 min). The binding mode study revealed that specific Pb2+–peptide interactions of the probes caused nanosized aggregates in which the fluorophores of the probes came close each other, exhibiting excimer emission. In particular, 8based on tetrapeptide bearing a disulfide bond and two carboxyl groups with a good permeability successfully quantified intracellular uptake of Pb2+in live cells through ratiometric fluorescent signals. The ratiometric sensing system based on specific metal–peptide interactions and excimer emission process could provide a valuable tool to quantify Pb2+in live cells and pure aqueous solutions.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results