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20 results on '"Lee, Jeehoon"'

7. Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization

Author

KANG, Ikjae, SEO, Hwi Won, PARK, Changhoon, OH, Yeonsu, LEE, Jeehoon, YOU, Ok Heui, KIM, Sung-Hoon, GOTTSCHALK, Marcelo, and CHAE, Chanhee

Subjects
DNA, Bacterial, Swine Diseases, Streptococcus suis, Swine, virus diseases, Biotin, Note, Familial Mediterranean Fever, Polyserositis, RNA, Ribosomal, 16S, Streptococcal Infections, Pathology, Animals, In situ hybridization, Digoxigenin
Abstract

The objective of this study was to develop digoxigenin-labeled in situ hybridization (ISH) for the detection of Streptococcus suis in naturally infected pigs with polyserositis and to compare it with biotinylated ISH. Digoxigenin-labeled hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis and microcolonies in the blood vessels. Mock hybridization showed no hybridization signals for endogenous digoxigenin. Biotinylated hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis. However, similar hybridization signals were also observed in the fibrous inflammatory area using mock hybridization for endogenous biotin. The present study demonstrated that digoxigenin-labeled ISH is a valuable diagnostic tool for specific detection of S. suis in polyserositic tissues without nonspecific reactions compared with biotinylated ISH.

Published
2013

19. Optimized protocol for multiplex nested polymerase chain reaction to detect and differentiate Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinis in formalin-fixed, paraffin-embedded tissues from pigs with polyserositis.

Author

Kang I, Kim D, Han K, Seo HW, Oh Y, Park C, Lee J, Gottschalk M, and Chae C

Subjects
Animals, Formaldehyde, Haemophilus Infections microbiology, Haemophilus Infections veterinary, Multiplex Polymerase Chain Reaction methods, Mycoplasma Infections microbiology, Mycoplasma Infections veterinary, Paraffin Embedding, Sensitivity and Specificity, Serositis microbiology, Streptococcal Infections microbiology, Streptococcal Infections veterinary, Swine, Swine Diseases diagnosis, Tissue Fixation, Haemophilus parasuis isolation & purification, Multiplex Polymerase Chain Reaction veterinary, Mycoplasma hyorhinis isolation & purification, Serositis veterinary, Streptococcus suis isolation & purification, Swine Diseases microbiology
Abstract

An optimized protocol was developed for the simultaneous detection and differentiation of Haemophilus parasuis, Streptococcus suis, and Mycoplasma hyorhinis in formalin-fixed, paraffin-embedded (FFPE) tissues with multiplex nested polymerase chain reaction (PCR). This method also determines the prevalence of these bacteria in pigs with polyserositis. DNA extraction with a combination of a commercial reagent and proteinase K resulted in more frequent detection of the pathogens than DNA extraction with proteinase K alone. Among FFPE tissue samples from 312 cases of polyserositis in which at least 1 bacterial species was detected, multiplex nested PCR detected H. parasuis in 239 (77%), S. suis in 124 (40%), and M. hyorhinis in 40 (13%). The disease was caused by a single pathogen in 224 (72%) of the cases and multiple pathogens in 88 (28%). Among the pigs positive for H. parasuis, S. suis, and M. hyorhinis by multiplex nested PCR, the pathogen was isolated from only 11%, 35%, and 28%, respectively. Therefore, the PCR protocol developed in this study is a useful diagnostic method when samples are negative after isolation methods and even for samples in which only 1 pathogen was isolated.

Published
2012

20. Distribution of capsular serotypes and virulence markers of Streptococcus suis isolated from pigs with polyserositis in Korea.

Author

Kim D, Han K, Oh Y, Kim CH, Kang I, Lee J, Gottschalk M, and Chae C

Subjects
Agglutination Tests veterinary, Animals, Antigens, Bacterial genetics, Antigens, Bacterial metabolism, Bacterial Capsules genetics, Bacterial Proteins genetics, Bacterial Proteins metabolism, DNA, Bacterial chemistry, DNA, Bacterial genetics, Hemolysin Proteins genetics, Hemolysin Proteins metabolism, Polymerase Chain Reaction veterinary, Serositis microbiology, Serotyping veterinary, Streptococcal Infections immunology, Streptococcal Infections microbiology, Streptococcus suis genetics, Swine, Swine Diseases immunology, Virulence, Bacterial Capsules metabolism, Serositis veterinary, Streptococcal Infections veterinary, Streptococcus suis pathogenicity, Swine Diseases microbiology
Abstract

The objective of this study was to determine the capsular serotypes and potential virulence factors of Streptococcus suis isolated from pigs with polyserositis. Among the 24 isolates evaluated, serotype 3 [7 (29%) of the isolates] and serotype 4 [5 (21%)] were the most common. The isolates were also studied for the presence of the genes mrp, epf, and sly, which encode muramidase-released protein (MRP), extracellular factor (EF), and suilysin (SLY), respectively. Of the 24 isolates, 8 carried mrp: 4 of serotype 3, 2 of serotype 2, and 2 of serotype 4. One mrp(+) isolate (serotype 2) also carried the epf gene. All 24 isolates carried the sly gene. The serotype and genotype distribution greatly differed from that reported for isolates from pigs with other clinical manifestations of S. suis infection in other countries.

Published
2010

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