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43 results on '"Lee, Shiao-Pieng"'

5. Targeting microRNA-190a halts the persistent myofibroblast activation and oxidative stress accumulation through upregulation of Krüppel-like factor 15 in oral submucous fibrosis.

Author

Chou, Ming-Yung, Lee, Chia-Hsuan, Hsieh, Pei-Ling, Chao, Shih-Chi, Yu, Chuan-Hang, Liao, Yi-Wen, Lee, Shiao-Pieng, Yu, Cheng-Chia, and Fan, Jun-Yang

Subjects
ORAL submucous fibrosis, GENE expression, KRUPPEL-like factors, REACTIVE oxygen species, PHENOTYPES
Abstract

Oral submucous fibrosis (OSF) is a condition characterized by inflammation and excessive collagen deposition, which has been identified as a potentially malignant disorder. Recently, several microRNAs (miRNAs) have been shown to be implicated in various disorders associated with fibrosis. However, how these miRNAs modulate OSF development is poorly understood. Therefore, the study aimed to identify the specific miRNAs that contribute to the progression of OSF and to investigate their molecular mechanisms in promoting fibrosis. The expression and clinical significance of potential pro-fibrosis miRNA in the OSF cohort and primary buccal mucosal fibroblasts were confirmed through RNA sequencing and qRT–PCR. Luciferase reporter activity assay, miRNA mimic or inhibitor, and short-hairpin RNA silencing were used to elucidate the molecular mechanism of miRNA. Transwell migration, collagen contraction, and reactive oxygen species (ROS) generation detection were used to investigate the effects of this mechanism on the myofibroblast phenotype and cellular pro-fibrosis capacity. This study demonstrated that miR-190a was overexpressed in fibrotic buccal mucosal fibroblasts (fBMFs). Transfecting fBMFs with miR-190a inhibitor resulted in reduced cell migration, collagen gel contraction, ROS generation, and expression of fibrotic markers. Furthermore, miR-190a exerted this pro-fibrosis property by direct binding to its target, Krüppel-like factor 15 (KLF15). The results also indicated that the aberrant upregulation of miR-190a, in turn, downregulated the expression of KLF15, which resulted in the activation of myofibroblast. Our findings demonstrated that miR-190a was involved in myofibroblast activation, suggesting that targeting the miR-190a/KLF15 axis may be a feasible approach in the therapy of OSF. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Characterization of intracellular buffering power in human induced pluripotent stem cells and the loss of pluripotency is delayed by acidic stimulation and increase of NHE1 activity

Author

Lee, Shiao‐Pieng, primary, Chao, Shih‐Chi, additional, Chou, Mei‐Fang, additional, Huang, Shu‐Fu, additional, Dai, Niann‐Tzyy, additional, Wu, Gwo‐Jang, additional, Tsai, Chien‐Sung, additional, Loh, Shih‐Hurng, additional, and Tsai, Yi‐Ting, additional

Published
2020
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26. Effects of Andrographolide on Intracellular pH Regulation, Cellular Migration, and Apoptosis in Human Cervical Cancer Cells (Running Tittle: Effects of Andrographolide on pH Regulators and Apoptosis in Cervical Cancer)

Author

Loh, Shih-Hurng, primary, Tsai, Yi-Ting, additional, Huang, Shu-Fu, additional, Yu, Tien-Chieh, additional, Kuo, Pei-Chun, additional, Chao, Shih-Chi, additional, Chou, Mei-Fang, additional, Tsai, Chien-Sung, additional, and Lee, Shiao-Pieng, additional

Published
2020
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30. Characterization of intracellular buffering power in human induced pluripotent stem cells and the loss of pluripotency is delayed by acidic stimulation and increase of NHE1 activity.

Author

Lee, Shiao‐Pieng, Chao, Shih‐Chi, Chou, Mei‐Fang, Huang, Shu‐Fu, Dai, Niann‐Tzyy, Wu, Gwo‐Jang, Tsai, Chien‐Sung, Loh, Shih‐Hurng, and Tsai, Yi‐Ting

Subjects
*INDUCED pluripotent stem cells, *PLURIPOTENT stem cells
Abstract

The homeostasis of intracellular pH (pHi) affects many cellular functions. Our previous study has established a functional and molecular model of the active pHi regulators in human induced pluripotent stem cells (hiPSCs). The aims of the present study were to further quantify passive pHi buffering power (β) and to investigate the effects of extracellular pH and Na+–H+ exchanger 1 (NHE1) activity on pluripotency in hiPSCs. pHi was detected by microspectrofluorimetry with pH‐sensitive dye‐BCECF. Western blot, immunofluorescence staining, and flow cytometry were used to detect protein expression and pluripotency. Our study in hiPSCs showed that (a) the value of total (βtot), intrinsic (βi), and CO2‐dependent (βCO2) buffering power all increased while pHi increased; (b) during the spontaneous differentiation for 4 days, the β values of βtot and βCO2 changed in a tendency of decrease, despite the absence of statistical significance; (c) an acidic cultured environment retained pluripotency and further upregulated expression and activity of NHE1 during spontaneous differentiation; (d) inhibition on NHE1 activity promoted the loss of pluripotency. In conclusion, we, for the first time, established a quantitative model of passive β during differentiation and demonstrated that maintenance of NHE1 at a higher level was of critical importance for pluripotency retention in hiPSCs. [ABSTRACT FROM AUTHOR]

Published
2021
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33. Correction: Tissue distribution and subcellular localizations determine in vivo functional relationship among prostasin, matriptase, HAI-1, and HAI-2 in human skin

Author

Lee, Shiao-Pieng, primary, Kao, Chen-Yu, additional, Chang, Shun-Cheng, additional, Chiu, Yi-Lin, additional, Chen, Yen-Ju, additional, Chen, Ming-Hsing G., additional, Chang, Chun-Chia, additional, Lin, Yu-Wen, additional, Chiang, Chien-Ping, additional, Wang, Jehng- Kang, additional, Lin, Chen-Yong, additional, and Johnson, Michael D., additional

Published
2018
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34. Tissue distribution and subcellular localizations determine in vivo functional relationship among prostasin, matriptase, HAI-1, and HAI-2 in human skin

Author

Lee, Shiao-Pieng, primary, Kao, Chen-Yu, additional, Chang, Shun-Cheng, additional, Chiu, Yi-Lin, additional, Chen, Yen-Ju, additional, Chen, Ming-Hsing G., additional, Chang, Chun-Chia, additional, Lin, Yu-Wen, additional, Chiang, Chien-Ping, additional, Wang, Jehng-Kang, additional, Lin, Chen-Yong, additional, and Johnson, Michael D., additional

Published
2018
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41. Matriptase Expression and Zymogen Activation in Human Pilosebaceous Unit

Author

Wu, Bai-Yao, primary, Lee, Shiao-Pieng, additional, Hsiao, Hui-Chung, additional, Chiu, Han, additional, Chen, Chi-Yung, additional, Yeo, Yee Hui, additional, Lee, Herng-Sheng, additional, Chen, Ya-Wen, additional, Kaul, Malvika, additional, Kataoka, Hiroaki, additional, Johnson, Michael D., additional, Wang, Jehng-Kang, additional, and Lin, Chen-Yong, additional

Published
2013
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42. Matriptase Expression and Zymogen Activation in Human Pilosebaceous Unit.

Author

Wu, Bai-Yao, Lee, Shiao-Pieng, Hsiao, Hui-Chung, Chiu, Han, Chen, Chi-Yung, Yeo, Yee Hui, Lee, Herng-Sheng, Chen, Ya-Wen, Kaul, Malvika, Kataoka, Hiroaki, Johnson, Michael D., Wang, Jehng-Kang, and Lin, Chen-Yong

Abstract

Studies of human genetic disorders and mouse models reveal the important roles of matriptase in hair growth. Here, we investigate matriptase expression and zymogen activation in hair follicles. We show: 1) layer-dependent distribution patterns, with much higher matriptase expression in cells of the outer root sheath and matrix cells of the hair bulb than in cells of the inner root sheath; 2) cycle-dependent expression patterns, with matriptase expressed in the anagen and catagen phases of the hair lifecycle, but not in the telogen phase; 3) reduced expression of the matriptase inhibitor, HAI-1, in the catagen phase, suggesting increased proteolytic activity in this phase; and 4) definitive matriptase zymogen activation patterns, with the highest matriptase activation observed in matrix cells and outer root sheath cells in the isthmus/bulge region. In sebaceous glands, matriptase is highly expressed in basal and ductal cells, with much lower expression in the differentiated, lipid-filled cells of the interior. We also show that matriptase potently activates hepatocyte growth factor (HGF) in vitro, and that the HGF receptor, c-Met, is co-expressed in those cells that express activated matriptase. Our observations suggest that the matriptase-HGF-c-MET pathway has the potential to be engaged, primarily in proliferative cells rather than terminally differentiated epithelial cells of the human pilosebaceous unit. [ABSTRACT FROM PUBLISHER]

Published
2014
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