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183 results on '"Leflon-Guibout, Véronique"'

5. Analysis of 15 cases from a monocentric cohort of 307 liver abscesses

Author

Canouï, Etienne, primary, Rossi, Geoffrey, additional, Nguyen, Yann, additional, Lafont, Emmanuel, additional, Rossi, Benjamin, additional, Roux, Olivier, additional, Dokmak, Safi, additional, Bert, Frédéric, additional, Leflon‐Guibout, Véronique, additional, Fantin, Bruno, additional, and Lefort, Agnès, additional

Published
2023
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6. Predominance of healthcare-associated cases among episodes of community-onset bacteraemia due to extended-spectrum β-lactamase-producing Enterobacteriaceae

Author

Aissa, Nejla, Losniewski, Alain, Henard, Sandrine, Rabaud, Christian, Janssen, Cécile, Pagani, Léonardo, Bland, Stéphane, Gaillat, Jacques, Bru, Jean Pierre, Bonnin, Pierre, Clavère, Gaelle, Vitrat, Virginie, Maulin, Laurence, Quarsaet, Luc, Roger, Helene, Euzen, Jean-Baptiste, Denes, Eric, Couve-Daecon, Elodie, Lechiche, Catherine, Lavigne, Jean-Philippe, Mondain, Véronique, Pulcini, Celine, Garnier, Veronique, Lambert, Dorothée, Hentzien, Maxime, Nguyen, Yohan, Talarmin, Jean Paul, Rameau, Pascaline, Geffroy, Françoise, Salmon-Rousseau, Arnaud, Cattoir, Vincent, Froissart, Antoine, Aberrane, Said, Legout, Laurence, Roblot, France, Beraud, Guillaume, Pavese, Patricia, Belmonte, Olivier, Traversier, Nicolas, Bellec, Laurent, Lagrange-Xelot, Marie, Moiton, Marie-Pierre, Kuli, Barbara, Longuet, Pascal, Scanvic, Agnes, Colombain, Lea, Ferreyra, Milagros, Jean, Maxime, Eden, Aurelia, Laurens, Etienne, Saada, Matthieu, Aumaitre, Hugues, Malet, Martine, Roustant, Frederique, LeMoing, Vincent, Helene, Jean-Pierre, Theodore, Jonathan, Garandeau, Caroline, Caillon, Jocelyne, Olivero, Benjamin, Boutoille, David, Lepelletier, Didier, Grandière-Perez, Lucia, Beaudron, Aurélie, Ramanantsoa, Céline, Varache, Catherine, Varache, Nicolas, Crochette, Nicolas, Hitoto, Hikombo, Blanchi, Sophie, Penn, Pascale, Louis, Bernard, Vanjak, Dominique, Escande, Marie-Christine, Patrigeon, René-Gilles, Honoré, Stéphanie, Barrelet, Audrey, Amara, Marlène, Galempoix, Jean-Marc, Réveil, Jean-Claude, Bucchiotty, Patricia, Couzigou, Carine, LeMonnier, Alban, Boidin, Emilie, Sire, Stephane, Luizy, Nelly, Bronstain, Caroline, Harbarth, Stephan, Fankhauser, Carolina, Brossier, Caroline, von Dach, Elodie, Sauve, Colette, Adam, Marie-Noelle, Hocqueloux, Laurent, Bret, Laurent, André, Marie-Hélène, Barruet, Régine, Challier, Marion, Cabié, Andre, Roze, Benoit, Plesiat, Patrick, Jeanot, Katy, Patry, Isabelle, Moreau, Joséphine, Chirouze, Catherine, Laurent, Aaron, Bachelier, Marie-Nadège, Kac, Guillaume, Farthouat, Nicolas, Feuillebois, Nicolas, Guimard, Thomas, Bourdon, Sandra, Nguyen, Sophie, Alfandari, Serge, Bentayeb, Houcine, Albertini, Mari-Thérèse, Marchou, Bruno, Gaudré, Noémie, Dubois, Damien, Guillet-Caruba, Christelle, Doucet Populaire, Florence, Fortineau, Nicolas, Escaut, Leila, Lesprit, Philippe, Fihman, Vincent, Gauzit, Rémy, Raymond, Josette, Kerneis, Solene, Leflon-Guibout, Veronique, Lefort, Agnes, Dinh, Aurelien, Roux, Anne-Laure, Abgrall, Sophie, Jauréguy, Françoise, Lucet, Jean-Christophe, Soismier, Nathalie, Lopes, Amanda, Cambau, Emmanuelle, Manceron, Veronique, Barnaud, Guilène, Zahar, Jean-Ralph, Ruckly, Stephane, Hikombo, Hitoto, Bernard, Louis, Timsit, Jean-François, and Brun-Buisson, Christian

Published
2017
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7. Contribution of Clinical Metagenomics to the Diagnosis of Bone and Joint Infections

Author

d’Humières, Camille, primary, Gaïa, Nadia, additional, Gueye, Signara, additional, de Lastours, Victoire, additional, Leflon-Guibout, Véronique, additional, Maataoui, Naouale, additional, Duprilot, Marion, additional, Lecronier, Marie, additional, Rousseau, Marc-Antoine, additional, Gamany, Naura, additional, Lescure, François-Xavier, additional, Senard, Olivia, additional, Deconinck, Laurène, additional, Dollat, Marion, additional, Isernia, Valentina, additional, Le Hur, Anne-Claire, additional, Petitjean, Marie, additional, Nazimoudine, Anissa, additional, Le Gac, Sylvie, additional, Chalal, Solaya, additional, Ferreira, Stéphanie, additional, Lazarevic, Vladimir, additional, Guigon, Ghislaine, additional, Gervasi, Gaspard, additional, Armand-Lefèvre, Laurence, additional, Schrenzel, Jacques, additional, and Ruppé, Etienne, additional

Published
2022
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11. Endocarditis caused by Thalassospira sp

Author

Deconinck, Laurène, Gschwind, Rémi, Petitjean, Marie, Gueye, Signara, Leflon-Guibout, Véronique, Maataoui, Naouale, Rondinaud, Emilie, Suard, Augustin, Gallais, Katell, Richaud, Rainui, Fuchs, Adeline, Iung, Bernard, Alkhoder, Soleiman, Ismaël, Sophie, Herrou, Julia, Prié, Héloïse, Armand-Lefèvre, Laurence, d’Humières, Camille, and Ruppé, Etienne

Subjects
Next-Generation Sequencing, Nanopore sequencing, Thalassospira, Case Report, Infectious and parasitic diseases, RC109-216, Infective endocarditis
Abstract

Highlights • First infective endocarditis by Thalassospira, a marine bacterium. • The patient is a fisherman who likely acquired the bacterium via extensive time spend in marine waters. • The strain could be cultured and identified by DNA sequencing methods, but not by routine mass spectrometry., We report a case of an infective endocarditis caused by a Thalassospira sp. in a 53-year-old man with pre-existing valvular lesions and living in French Polynesia as a fisherman. The strain was identified with DNA-sequecing methods while it was not by mass spectrometry.

Published
2021

12. Prevalence of day-care centre children (France) with faecal CTX-M-producing Escherichia coli comprising O25b:H4 and O16:H5 ST131 strains

Author

Blanc, Véronique, Leflon-Guibout, Véronique, Blanco, Jorge, Haenni, Marisa, Madec, Jean-Yves, Rafignon, Gwenaële, Bruno, Pascale, Mora, Azucena, Lopez, Cecilia, Dahbi, Ghizlane, Dunais, Brigitte, Anastay, Magali, Branger, Catherine, Moreau, Richard, Pradier, Christian, and Nicolas-Chanoine, Marie-Hélène

Published
2014
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13. Contribution of Clinical Metagenomics to the Diagnosis of Bone and Joint Infections.

Author

d'Humières, Camille, Gaïa, Nadia, Gueye, Signara, de Lastours, Victoire, Leflon-Guibout, Véronique, Maataoui, Naouale, Duprilot, Marion, Lecronier, Marie, Rousseau, Marc-Antoine, Gamany, Naura, Lescure, François-Xavier, Senard, Olivia, Deconinck, Laurène, Dollat, Marion, Isernia, Valentina, Le Hur, Anne-Claire, Petitjean, Marie, Nazimoudine, Anissa, Le Gac, Sylvie, and Chalal, Solaya

Subjects
JOINT infections, METAGENOMICS, MICROBIAL cultures, NUCLEIC acids, RECOMBINANT DNA, DIAGNOSIS
Abstract

Bone and joint infections (BJIs) are complex infections that require precise microbiological documentation to optimize antibiotic therapy. Currently, diagnosis is based on microbiological culture, sometimes complemented by amplification and sequencing of the 16S rDNA gene. Clinical metagenomics (CMg), that is, the sequencing of the entire nucleic acids in a sample, was previously shown to identify bacteria not detected by conventional methods, but its actual contribution to the diagnosis remains to be assessed, especially with regard to 16S rDNA sequencing. In the present study, we tested the performance of CMg in 34 patients (94 samples) with suspected BJIs, as compared to culture and 16S rDNA sequencing. A total of 94 samples from 34 patients with suspicion of BJIs, recruited from two sites, were analyzed by (i) conventional culture, (ii) 16S rDNA sequencing (Sanger method), and (iii) CMg (Illumina Technology). Two negative controls were also sequenced by CMg for contamination assessment. Based on the sequencing results of negative controls, 414 out of 539 (76.7%) bacterial species detected by CMg were considered as contaminants and 125 (23.2%) as truly present. For monomicrobial infections (13 patients), the sensitivity of CMg was 83.3% as compared to culture, and 100% as compared to 16S rDNA. For polymicrobial infections (13 patients), the sensitivity of CMg was 50% compared to culture, and 100% compared to 16S rDNA. For samples negative in culture (8 patients, 21 samples), CMg detected 11 bacteria in 10 samples from 5 different patients. In 5/34 patients, CMg brought a microbiological diagnosis where conventional methods failed, and in 16/34 patients, CMg provided additional information. Finally, 99 antibiotic resistance genes were detected in 24 patients (56 samples). Provided sufficient genome coverage (87.5%), a correct inference of antibiotic susceptibility was achieved in 8/8 bacteria (100%). In conclusion, our study demonstrated that the CMg provides complementary and potentially valuable data to conventional methods of BJIs diagnosis. [ABSTRACT FROM AUTHOR]

Published
2022
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18. Molecular epidemiology of Escherichia coli producing extended-spectrum β-lactamases in Lugo (Spain): dissemination of clone O25b:H4-ST131 producing CTX-M-15

Author

Blanco, Miguel, Alonso, Maria Pilar, Nicolas-Chanoine, Marie-Hélène, Dahbi, Ghizlane, Mora, Azucena, Blanco, Jesús E., López, Cecilia, Cortés, Pilar, Llagostera, Montserrat, Leflon-Guibout, Véronique, Puentes, Beatriz, Mamani, Rosalía, Herrera, Alexandra, Coira, María Amparo, García-Garrote, Fernando, Pita, Julia María, and Blanco, Jorge

Published
2009

23. A prospective, observational study of fidaxomicin use for Clostridioides difficile infection in France.

Author

Guery, Benoit, Berger, Pierre, Gauzit, Remy, Gourdon, Magali, Barbut, Frédéric, DAFNE study group:, Bémer, Pascale, Bessède, Emilie, Camou, Fabrice, Cattoir, Vincent, Couzigou, Carine, Descamps, Dominique, Dinh, Aurélien, Laurans, Caroline, Lavigne, Jean-Philippe, Lechiche, Catherine, Leflon-Guibout, Véronique, Le Monnier, Alban, Levast, Marion, and Mootien, Joy Yoganaden

Published
2021
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25. Additional file 2: Table S2. of The ST131 Escherichia coli H22 subclone from human intestinal microbiota: Comparison of genomic and phenotypic traits with those of the globally successful H30 subclone

Author

Marie-Hélène Nicolas-Chanoine, Petitjean, Marie, Mora, Azucena, Mayer, Noémie, Jean-Philippe Lavigne, Boulet, Olivier, Leflon-Guibout, Véronique, Blanco, Jorge, and Hocquet, Didier

Abstract

Escherichia coli virulence factor encoding genes tested by the PCR method. (DOCX 25 kb)

Published
2017
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26. Additional file 3: Table S3. of The ST131 Escherichia coli H22 subclone from human intestinal microbiota: Comparison of genomic and phenotypic traits with those of the globally successful H30 subclone

Author

Marie-Hélène Nicolas-Chanoine, Petitjean, Marie, Mora, Azucena, Mayer, Noémie, Jean-Philippe Lavigne, Boulet, Olivier, Leflon-Guibout, Véronique, Blanco, Jorge, and Hocquet, Didier

Abstract

Distribution and identity percentage of the genes previously shown to be involved in biofilm formation [28] in representatives of different ST131 H subclones. (DOCX 41 kb)

Published
2017
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27. Additional file 4: Table S4. of The ST131 Escherichia coli H22 subclone from human intestinal microbiota: Comparison of genomic and phenotypic traits with those of the globally successful H30 subclone

Author

Marie-Hélène Nicolas-Chanoine, Petitjean, Marie, Mora, Azucena, Mayer, Noémie, Jean-Philippe Lavigne, Boulet, Olivier, Leflon-Guibout, Véronique, Blanco, Jorge, and Hocquet, Didier

Abstract

H22 and H30 strains with a whole genome sequence available in NCBI databases either under a circular DNA form or a fragmented DNA form. (DOCX 16 kb)

Published
2017
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28. Additional file 1: Table S1. of The ST131 Escherichia coli H22 subclone from human intestinal microbiota: Comparison of genomic and phenotypic traits with those of the globally successful H30 subclone

Author

Marie-Hélène Nicolas-Chanoine, Petitjean, Marie, Mora, Azucena, Mayer, Noémie, Jean-Philippe Lavigne, Boulet, Olivier, Leflon-Guibout, Véronique, Blanco, Jorge, and Hocquet, Didier

Abstract

List of primers designed and used in this study (DOCX 24 kb)

Published
2017
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29. HypervirulentKlebsiella pneumoniaein Cryptogenic Liver Abscesses, Paris, France

Author

Rossi, Benjamin, primary, Gasperini, Maria Ludovica, additional, Leflon-Guibout, Véronique, additional, Gioanni, Alice, additional, de Lastours, Victoire, additional, Rossi, Geoffrey, additional, Dokmak, Safi, additional, Ronot, Maxime, additional, Roux, Olivier, additional, Nicolas-Chanoine, Marie-Hélène, additional, Fantin, Bruno, additional, and Lefort, Agnès, additional

Published
2018
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30. The ST131 Escherichia coli H22 subclone from human intestinal microbiota: Comparison of genomic and phenotypic traits with those of the globally successful H30 subclone

Author

Nicolas-Chanoine, Marie-Hélène, primary, Petitjean, Marie, additional, Mora, Azucena, additional, Mayer, Noémie, additional, Lavigne, Jean-Philippe, additional, Boulet, Olivier, additional, Leflon-Guibout, Véronique, additional, Blanco, Jorge, additional, and Hocquet, Didier, additional

Published
2017
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31. Hypervirulent Klebsiella pneumoniae in Cryptogenic Liver Abscesses, Paris, France.

Author

Rossi, Benjamin, Gasperini, Maria Ludovica, Leflon-Guibout, Váronique, Gioanni, Alice, de Lastours, Victoire, Rossi, Geoffrey, Dokmak, Safi, Ronot, Maxime, Roux, Olivier, Nicolas-Chanoine, Marie-Hélène, Fantin, Bruno, Lefort, Agnès, and Leflon-Guibout, Véronique

Subjects
KLEBSIELLA pneumoniae, LIVER abscesses, CRYPTOGENIC organizing pneumonia, CANCER patients, MICROBIAL virulence
Abstract

Liver abscesses containing hypervirulent Klebsiella pneumoniae have emerged during the past 2 decades, originally in Southeast Asia and then worldwide. We hypothesized that hypervirulent K. pneumoniae might also be emerging in France. In a retrospective, monocentric, cohort study, we analyzed characteristics and outcomes for 199 consecutive patients in Paris, France, with liver abscesses during 2010-2015. We focused on 31 patients with abscesses containing K. pneumoniae. This bacterium was present in most (14/27, 52%) cryptogenic liver abscesses. Cryptogenic K. pneumoniae abscesses were more frequently community-acquired (p<0.00001) and monomicrobial (p = 0.008), less likely to involve cancer patients (p<0.01), and relapsed less often (p<0.01) than did noncryptogenic K. pneumoniae liver abscesses. K. pneumoniae isolates from cryptogenic abscesses belonged to either the K1 or K2 serotypes and had more virulence factors than noncryptogenic K. pneumoniae isolates. Hypervirulent K. pneumoniae are emerging as the main pathogen isolated from cryptogenic liver abscesses in the study area. [ABSTRACT FROM AUTHOR]

Published
2018
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32. Phylogenetic Distribution of CTX-M- and Non-Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates: Group B2 Isolates, Except Clone ST131, Rarely Produce CTX-M Enzymes

Author

Brisse, Sylvain, Diancourt, Laure, Laouénan, Cédric, Vigan, Marie, Caro, Valérie, Arlet, Guillaume, Drieux, Laurence, Leflon-Guibout, Véronique, Mentré, France, Jarlier, Vincent, and Nicolas-Chanoine, Marie-Hélène

Subjects
Microbiology (medical), Clone (cell biology), Biology, medicine.disease_cause, beta-Lactamases, Microbiology, Antibiotic resistance, Phylogenetics, Drug Resistance, Bacterial, medicine, polycyclic compounds, Escherichia coli, Prevalence, Cluster Analysis, Humans, Escherichia coli Infections, Phylogeny, Genetics, Molecular Epidemiology, Molecular epidemiology, Phylogenetic tree, Genetic Variation, Bacteriology, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Phylogenetic diversity, Multilocus sequence typing, Multilocus Sequence Typing
Abstract

Escherichia coli is the species most frequently associated with clinical infections by extended-spectrum-β-lactamase (ESBL)-producing isolates, with the CTX-M ESBL enzymes being predominant and found in genetically diverse E. coli isolates. The main objective of this study was to compare, on the basis of a case-control design, the phylogenetic diversity of 152 CTX-M-producing and 152 non-ESBL-producing clinical E. coli isolates. Multilocus sequence typing revealed that even though CTX-M enzymes were largely disseminated across the diversity of E. coli isolates, phylogenetic group B2 showed a particularly heterogeneous situation. First, clone ST131 of group B2 was strongly associated with CTX-M production (55 [79%] of 70 isolates), with CTX-M-15 being predominant. Second, the remaining members of group B2 were significantly less frequently associated with CTX-M production (9 [12%] of 75) than E. coli phylogenetic groups A, B1, and D (88 [55%] of 159). CTX-M-producing ST131 E. coli isolates were significantly more frequent in patients hospitalized in geriatric wards or long-term care facilities. Besides, the non-ESBL ST131 isolates significantly more frequently showed resistance to penicillins than the non-ESBL, non-ST131 isolates did. In conclusion, the present study emphasizes the particular antimicrobial resistance and epidemiologic characteristics of clone ST131 within group B2, which could result from the higher antibiotic exposure of this clone, as it is the predominant clone of group B2 carried in the human gut.

Published
2012

33. Erratum to ‘Predominance of healthcare-associated cases among episodes of community-onset bacteraemia due to extended-spectrum β-lactamase-producing Enterobacteriaceae’ [International Journal of Antimicrobial Agents 49/1 67–73]

Author

Brun-Buisson, Christian, Carlet, Jean, Dellamonica, Pierre, Gauzit, Remy, Rabaud, Christian, Zahar, Jean-Ralph, Timsit, Jean-Francois, Alfandari, Serge, Cohen, Robert, Coignard, Bruno, Pulcini, Celine, Robert, Jerome, Aissa, Nejla, Losniewski, Alain, Henard, Sandrine, Janssen, Cecile, Pagani, Leonardo, Bland, Stephane, Gaillat, Jacques, Bru, Jean Pierre, Bonnin, Pierre, Clavere, Gaelle, Vitrat, Virginie, Maulin, Laurence, Quarsaet, Luc, Roger, Helene, Euzen, Jean-Baptiste, Denes, Eric, Couve-Daecon, Elodie, Lechiche, Catherine, Lavigne, Jean-Philippe, Mondain, Veronique, Garnier, Veronique, Lambert, Dorothee, Hentzien, Maxime, Nguyen, Yohan, Talarmin, Jean Paul, Rameau, Pascaline, Geffroy, Francoise, Salmon-Rousseau, Arnaud, Cattoir, Vincent, Froissart, Antoine, Aberrane, Said, Legout, Laurence, Roblot, France, Beraud, Guillaume, Pavese, Patricia, Belmonte, Olivier, Traversier, Nicolas, Bellec, Laurent, Lagrange-Xelot, Marie, Moiton, Marie-Pierre, Kuli, Barbara, Longuet, Pascal, Scanvic, Agnes, Colombain, Lea, Ferreyra, Milagros, Jean, Maxime, Eden, Aurelia, Laurens, Etienne, Saada, Matthieu, Aumaitre, Hugues, Malet, Martine, Roustant, Frederique, LeMoing, Vincent, Helene, Jean-Pierre, Theodore, Jonathan, Garandeau, Caroline, Caillon, Jocelyne, Olivero, Benjamin, Boutoille, David, Lepelletier, Didier, Grandiere-Perez, Lucia, Beaudron, Aurelie, Ramanantsoa, Celine, Varache, Catherine, Varache, Nicolas, Crochette, Nicolas, Hitoto, Hikombo, Blanchi, Sophie, Penn, Pascale, Louis, Bernard, Vanjak, Dominique, Escande, Marie-Christine, Patrigeon, Rene-Gilles, Honore, Stephanie, Barrelet, Audrey, Amara, Marlene, Galempoix, Jean-Marc, Reveil, Jean-Claude, Bucchiotty, Patricia, Couzigou, Carine, LeMonnier, Alban, Boidin, Emilie, Sire, Stephane, Luizy, Nelly, Bronstain, Caroline, Harbarth, Stephan, Fankhauser, Carolina, Brossier, Caroline, von Dach, Elodie, Sauve, Colette, Adam, Marie-Noelle, Hocqueloux, Laurent, Bret, Laurent, Andre, Marie-Helene, Barruet, Regine, Challier, Marion, Cabie, Andre, Roze, Benoit, Plesiat, Patrick, Jeanot, Katy, Patry, Isabelle, Moreau, Josephine, Chirouze, Catherine, Laurent, Aaron, Bachelier, Marie-Nadege, Kac, Guillaume, Farthouat, Nicolas, Feuillebois, Nicolas, Guimard, Thomas, Bourdon, Sandra, Nguyen, Sophie, Bentayeb, Houcine, Albertini, Mari-Therese, Marchou, Bruno, Gaudre, Noemie, Dubois, Damien, Guillet-Caruba, Christelle, Populaire, Florence Doucet, Fortineau, Nicolas, Escaut, Leila, Lesprit, Philippe, Fihman, Vincent, Raymond, Josette, Kerneis, Solene, Leflon-Guibout, Veronique, Lefort, Agnes, Dinh, Aurelien, Roux, Anne-Laure, Abgrall, Sophie, Jaureguy, Francoise, Lucet, Jean-Christophe, Soismier, Nathalie, Lopes, Amanda, Cambau, Emmanuelle, Manceron, Veronique, Barnaud, Guilene, Ruckly, Stephane, Hikombo, Hitoto, and Bernard, Louis

Published
2017
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34. Genetic and Biochemical Characterization of the Chromosomal Class A β-Lactamases of Raoultella (formerly Klebsiella) planticola and Raoultella ornithinolytica

Author

Walckenaer, Estelle, Poirel, Laurent, Leflon-Guibout, Véronique, Nordmann, Patrice, and Nicolas-Chanoine, Marie-Hélène

Subjects
DNA, Bacterial, Enterobacteriaceae, Mechanisms of Resistance, Molecular Sequence Data, Amino Acid Sequence, Microbial Sensitivity Tests, Cloning, Molecular, Isoelectric Focusing, beta-Lactams, beta-Lactamases, Anti-Bacterial Agents, Plasmids
Abstract

Enterobacterial strains of Raoultella spp. display a penicillinase-related beta-lactam resistance pattern suggesting the presence of a chromosomal bla gene. From whole-cell DNA of Raoultella planticola strain ATCC 33531(T) and Raoultella ornithinolytica strain ATCC 31898(T), bla genes were cloned and expressed into Escherichia coli. Each gene encoded an Ambler class A beta-lactamase, named PLA-1 and ORN-1 for R. planticola and R. ornithinolytica, respectively. These beta-lactamases (291 amino acids), with the same pI value of 7.8, had a shared amino acid identity of 94%, 37 to 47% identity with the majority of the chromosome-encoded class A beta-lactamases previously described for Enterobacteriaceae, and 66 to 69% identity with the two beta-lactamases LEN-1 and SHV-1 from Klebsiella pneumoniae. However, the highest identity percentage (69 to 71%) was found with the plasmid-mediated beta-lactamase TEM-1. PLA-1, which displayed very strong hydrolytic activity against penicillins, also displayed significant hydrolytic activity against cefepime and, to a lesser extent, against cefotaxime and aztreonam, but there was no hydrolytic activity against ceftazidime. Such a substrate profile suggests that the Raoultella beta-lactamases PLA-1 and ORN-1 should be classified into the group 2be of the beta-lactamase classification of K. Bush, G. A. Jacoby, and A. A. Medeiros (Antimicrob. Agents Chemother. 39:1211-1233, 1995). The highly homologous regions upstream of the bla(PLA-1A) and bla(ORN-1A) genes comprised a nucleotide sequence identical to the -35 region and another one very close to the -10 region of the bla(LEN-1) gene. From now on, as the bla gene sequences of the most frequent Raoultella and Klebsiella species are available, the bla gene amplification method can be used to differentiate these species from each other, which the biochemical tests currently carried out in the clinical laboratory are unable to do.

Published
2004

36. Promoters P3, Pa/Pb, P4, and P5 Upstream from blaTEM Genes and Their Relationship to β-Lactam Resistance

Author

Lartigue, Marie Frédérique, Leflon-Guibout, Véronique, Poirel, Laurent, Nordmann, Patrice, and Nicolas-Chanoine, Marie-Hélène

Subjects
Base Sequence, Mechanisms of Resistance, Escherichia coli, Microbial Sensitivity Tests, Promoter Regions, Genetic, beta-Lactams, Gene Expression Regulation, Enzymologic, beta-Lactamases, Anti-Bacterial Agents
Abstract

Using an isogenic system, we have determined the impact that the four promoters known to control bla(TEM) gene expression have on beta-lactamase activity. For both TEM-1 and TEM-30, this activity gradually increased in relation to the presence of promoters P3, Pa/Pb, and P4 upstream of the corresponding gene. Promoter P5, only found upstream of the bla(TEM-1B) gene, was related to the highest expression of this gene.

Published
2002

38. Clinical Outcome After a Totally Implantable Venous Access Port-Related Infection in Cancer Patients

Author

Lebeaux, David, primary, Larroque, Béatrice, additional, Gellen-Dautremer, Justine, additional, Leflon-Guibout, Véronique, additional, Dreyer, Chantal, additional, Bialek, Suzanne, additional, Froissart, Antoine, additional, Hentic, Olivia, additional, Tessier, Catherine, additional, Ruimy, Raymond, additional, Pelletier, Anne-Laure, additional, Crestani, Bruno, additional, Fournier, Michel, additional, Papo, Thomas, additional, Barry, Béatrix, additional, Zarrouk, Virginie, additional, and Fantin, Bruno, additional

Published
2012
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40. CTX-M-15-Producing Escherichia coli Clinical Isolates in Cairo (Egypt), Including Isolates of Clonal Complex ST10 and Clones ST131, ST73, and ST405 in Both Community and Hospital Settings

Author

Fam, Nevine, primary, Leflon-Guibout, Véronique, additional, Fouad, Salwa, additional, Aboul-Fadl, Laila, additional, Marcon, Estelle, additional, Desouky, Doaa, additional, El-Defrawy, Inas, additional, Abou-Aitta, Aisha, additional, Klena, John, additional, and Nicolas-Chanoine, Marie-Hélène, additional

Published
2011
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43. Genetic Diversity and Virulence Profiles of Escherichia coli Isolates Causing Spontaneous Bacterial Peritonitis and Bacteremia in Patients with Cirrhosis

Author

Bert, Frédéric, primary, Johnson, James R., additional, Ouattara, Bénédicte, additional, Leflon-Guibout, Véronique, additional, Johnston, Brian, additional, Marcon, Estelle, additional, Valla, Dominique, additional, Moreau, Richard, additional, and Nicolas-Chanoine, Marie-Hélène, additional

Published
2010
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48. Emergence and Spread of Three Clonally Related Virulent Isolates of CTX-M-15-Producing Escherichia coli with Variable Resistance to Aminoglycosides and Tetracycline in a French Geriatric Hospital

Author

Leflon-Guibout, Véronique, primary, Jurand, Cécile, additional, Bonacorsi, Stéphane, additional, Espinasse, Florence, additional, Guelfi, Marie Claude, additional, Duportail, Françoise, additional, Heym, Beate, additional, Bingen, Edouard, additional, and Nicolas-Chanoine, Marie-Hélène, additional

Published
2004
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