98 results on '"Lei, Anna"'
Search Results
2. Basomedial amygdala mediates top-down control of anxiety and fear.
- Author
-
Adhikari, Avishek, Lerner, Talia N, Finkelstein, Joel, Pak, Sally, Jennings, Joshua H, Davidson, Thomas J, Ferenczi, Emily, Gunaydin, Lisa A, Mirzabekov, Julie J, Ye, Li, Kim, Sung-Yon, Lei, Anna, and Deisseroth, Karl
- Subjects
Amygdala ,Prefrontal Cortex ,Neural Pathways ,Animals ,Mice ,Inbred C57BL ,Mice ,Freezing Reaction ,Cataleptic ,Stress ,Psychological ,Anxiety ,Fear ,Learning ,Female ,Male ,Extinction ,Psychological ,Extinction ,Psychological ,Freezing Reaction ,Cataleptic ,Inbred C57BL ,Stress ,General Science & Technology - Abstract
Anxiety-related conditions are among the most difficult neuropsychiatric diseases to treat pharmacologically, but respond to cognitive therapies. There has therefore been interest in identifying relevant top-down pathways from cognitive control regions in medial prefrontal cortex (mPFC). Identification of such pathways could contribute to our understanding of the cognitive regulation of affect, and provide pathways for intervention. Previous studies have suggested that dorsal and ventral mPFC subregions exert opposing effects on fear, as do subregions of other structures. However, precise causal targets for top-down connections among these diverse possibilities have not been established. Here we show that the basomedial amygdala (BMA) represents the major target of ventral mPFC in amygdala in mice. Moreover, BMA neurons differentiate safe and aversive environments, and BMA activation decreases fear-related freezing and high-anxiety states. Lastly, we show that the ventral mPFC-BMA projection implements top-down control of anxiety state and learned freezing, both at baseline and in stress-induced anxiety, defining a broadly relevant new top-down behavioural regulation pathway.
- Published
- 2015
3. The Catalytic and Non-catalytic Functions of the Brahma Chromatin-Remodeling Protein Collaborate to Fine-Tune Circadian Transcription in Drosophila.
- Author
-
Kwok, Rosanna S, Li, Ying H, Lei, Anna J, Edery, Isaac, and Chiu, Joanna C
- Subjects
Cell Line ,Animals ,Drosophila melanogaster ,RNA Polymerase II ,Cell Cycle Proteins ,DNA-Binding Proteins ,Trans-Activators ,Drosophila Proteins ,Chromatin Assembly and Disassembly ,Transcription ,Genetic ,Gene Expression Regulation ,Binding Sites ,Circadian Rhythm ,Promoter Regions ,Genetic ,CLOCK Proteins ,Period Circadian Proteins ,Promoter Regions ,Genetic ,Transcription ,Genetics ,Developmental Biology - Abstract
Daily rhythms in gene expression play a critical role in the progression of circadian clocks, and are under regulation by transcription factor binding, histone modifications, RNA polymerase II (RNAPII) recruitment and elongation, and post-transcriptional mechanisms. Although previous studies have shown that clock-controlled genes exhibit rhythmic chromatin modifications, less is known about the functions performed by chromatin remodelers in animal clockwork. Here we have identified the Brahma (Brm) complex as a regulator of the Drosophila clock. In Drosophila, CLOCK (CLK) is the master transcriptional activator driving cyclical gene expression by participating in an auto-inhibitory feedback loop that involves stimulating the expression of the main negative regulators, period (per) and timeless (tim). BRM functions catalytically to increase nucleosome density at the promoters of per and tim, creating an overall restrictive chromatin landscape to limit transcriptional output during the active phase of cycling gene expression. In addition, the non-catalytic function of BRM regulates the level and binding of CLK to target promoters and maintains transient RNAPII stalling at the per promoter, likely by recruiting repressive and pausing factors. By disentangling its catalytic versus non-catalytic functions at the promoters of CLK target genes, we uncovered a multi-leveled mechanism in which BRM fine-tunes circadian transcription.
- Published
- 2015
4. Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Gollapudi, Anantha, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Ramamoorthy, Preveen
- Published
- 2020
- Full Text
- View/download PDF
5. PROGETTARE INFRASTRUTTURE FLESSIBILI
- Author
-
Caravaggi, Lucina, primary, Imbroglini, Cristina, additional, and Lei, Anna, additional
- Published
- 2019
- Full Text
- View/download PDF
6. Einsatz von Integraseinhibitoren in der Schwangerschaft
- Author
-
Weißmann, Daniela Lei-Anna Martina and Weißmann, Daniela Lei-Anna Martina
- Abstract
Integraseinhibitoren sind bereits seit Jahren in der Initialtherapie HIV-positiver Erwachsener empfohlen, in der Schwangerschaft aber bisher aber meist nur unter besonderen Voraussetzungen (z.B. bei hoher maternaler Viruslast kurz vor Entbindung) eingesetzt. Ziel dieser retrospektiven Studie ist es, die Effektivität und Verträglichkeit integraseinhibitorhaltiger ART bei schwangeren Patientinnen zu untersuchen und diese mit integraseinhibitorfreien Regimen zu vergleichen. Die quantitative Viruslast bzw. die Suppression der Viruslast zum Zeitpunkt der Entbindung stellte die primäre Zielgröße der Studie dar, Indikationen für einen INSTI als Intensivierung der ART oder Gründe für einen Therapiewechsel sowie unerwünschte Wirkungen bei Mutter und Kind waren sekundäre Zielgrößen. Hierzu wurden retrospektive Daten aus der Epidem-Datenbank des HIVCENTERs und aus den Patientinnenakten der teilnehmenden Frankfurter Schwerpunktpraxen extrahiert und in die Auswertung einbezogen. Im Beobachtungzeitraum vom 1. Januar 2008 bis zum 01. Juni 2018 wurden n=274 Schwangerschaften erfasst, diese resultierten in n=281 Kindern (fünf Zwillinge und einmal Drillinge). INSTI-haltige Therapieregime wurden bei 52 (19%) der Schwangerschaften eingesetzt, darunter zumeist Raltegravir (92%). Die maternale Viruslast zum Zeitpunkt der Geburt unterschied sich in beiden Therapiegruppen nicht signifikant, in beiden Subgruppen konnte eine signifikante Reduktion der Viruslast zum Zeitpunkt der Entbindung erreicht werden., Integrase inhibitors are highly recommended for the initial treatment of HIV-positive adults, but have so far only been used during pregnancy under special conditions (e.g. high maternal viral load shortly before delivery). The aim of this retrospective study is to investigate the effectiveness and tolerability of integrase inhibitor-containing ART in pregnant patients and to compare these with integrase inhibitor-free regimens. The primary objective of this study was the quantitative viral load respectively virologic suppression at time of delivery; secondary objectives were indications for an INSTI containing ART, reasons for a change of regimen as well as adverse effects in mother and child. For this purpose, retrospective data from the HIVCENTER’s Epidem database and patient files of the participating clinics in Frankfurt were extracted and included in the analysis. From January 1, 2008 to June 1, 2018 we observed n=274 pregnancies resulting in n=281 children (five twins and one triplet). INSTI-containing regimen were used in 52 (19%) pregnancies, predominantly raltegravir (92%). There were no significant differences in terms of virologic response between the two treatment groups; a significant reduction of the viral load at the time of delivery was achieved in both subgroups.
- Published
- 2023
7. Cartes, images et dessins dans la démarche de co-conception du paysage. L’expérience de l’atelier Pontons Corviale à Rome
- Author
-
Lei, Anna and Caravaggi, Lucina
- Subjects
progetto di paesaggio contemporaneo ,rappresentazione del paesaggio ,progettazione partecipata ,Pontili Corviale ,accessibilità metropolitana ,Roma - Published
- 2023
8. Giardini viventi. Coltivare la città per curare il mondo
- Author
-
Lei, Anna
- Subjects
edible garden ,progetto di paesaggio contemporaneo ,community garden ,jardin vivant ,coltivazione ,giardino ,cura - Published
- 2023
9. Animali in città
- Author
-
Imbroglini, Cristina, primary and Lei, Anna, additional
- Published
- 2023
- Full Text
- View/download PDF
10. Animali in città
- Author
-
Lei, Anna, primary and Imbroglini, Cristina, additional
- Published
- 2023
- Full Text
- View/download PDF
11. Progettare la lentezza. Le ragioni del progetto culturale di linee antifragili
- Author
-
Lei, Anna, primary
- Published
- 2022
- Full Text
- View/download PDF
12. A unified peptide array platform for antibody epitope binning, mapping, specificity and predictive off-target binding
- Author
-
Moore, Cody, primary, Lei, Anna, additional, Walsh, Patrick, additional, Trenchevska, Olgica, additional, Saini, Gaurav, additional, Tarasow, Theodore M, additional, Srinivasan, Mohan, additional, Smith, David, additional, and Greving, Matthew P, additional
- Published
- 2022
- Full Text
- View/download PDF
13. Prenatal ethanol exposure impairs sensory processing and habituation of visual stimuli, effects normalized by postnatal environmental enrichment
- Author
-
Wang, Ruixiang, primary, Martin, Connor D., additional, Lei, Anna L., additional, Hausknecht, Kathryn A., additional, Turk, Marisa, additional, Micov, Veronika, additional, Kwarteng, Francis, additional, Ishiwari, Keita, additional, Oubraim, Saida, additional, Wang, An‐Li, additional, Richards, Jerry B., additional, Haj‐Dahmane, Samir, additional, and Shen, Roh‐Yu, additional
- Published
- 2022
- Full Text
- View/download PDF
14. Infrastrutture di paesaggio - categorie per il progetto di nuovi spazi lineari
- Author
-
Caravaggi, Lucina and Lei, Anna
- Subjects
infrastrutture di paesaggio ,diffusione insediativa ,civic ecology ,mobilità sostenibile ,walkability - Published
- 2022
15. Rome’s GRAB—Great Bicycle Ring Route—As Complex Landscape Infrastructure
- Author
-
Caravaggi, Lucina, primary, Imbroglini, Cristina, additional, and Lei, Anna, additional
- Published
- 2022
- Full Text
- View/download PDF
16. Sugar wonders
- Author
-
Best, Andrew, Van Der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, muo, Lohmann, Julia, Correia Alves, Carlos, Best, Andrew, Van Der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, muo, Lohmann, Julia, and Correia Alves, Carlos
- Abstract
This work has sugar at its core. Sugar is nowadays an omnipresent substance in processed food due to its taste enhancing qualities. We can find sugar in food items that we would not consider sugary; for instance, soups, bread, and packed nuts among many others. Excessive consumption of added sugar may lead to many health and well-being problems. The most known condition associated with sugar consumption is diabetes, the ninth leading cause of death in the world. When at a young age, the author was diagnosed with diabetes, so minding sugar consumption became an essential aspect of his life. Initially, this was a personal concern, which afterwards, served as a motivation for his artistic persona. This MA thesis is a gathering of personal considerations that are reasoned on empirical experiences and facts. It concerns social awareness in the context of sugar consumption and diabetes. In order to convey these considerations, a series of objects which have sugar as their main medium, were produced. The conception of these objects was enhanced by a combination of theoretical research and material exploration. The theoretical research assembled notions from the fields of social psychology (with a focus on empathy) and speculative design. The material exploration consisted of exploring sugar as a building material by applying it to conventional craft methods and digitalized manufacturing methods. Through the lens of material development, these material explorations confirmed the potential of sugar as a building material. From a narrative perspective, what better medium than sugar itself, to expand on the problem of its consumption and to bring awareness about diabetes. Furthermore, SUGAR WONDERS allowed the author to find new branch topics, such as Diabetia, the country, which expands on the idea of a country made of diabetics and the notion of belonging for a person with a condition.
- Published
- 2021
17. Aesthetics of chaos - Exploring creative identity through material and colour experiments with Finnish clay
- Author
-
van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, muo, Mäkelä, Maarit, Eren, Hulya, van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, muo, Mäkelä, Maarit, and Eren, Hulya
- Abstract
Aesthetics of Chaos is a study on exploring creative identity, using Finnish clay as its main medium. The starting point of this research work is author’s critical self-reflection on her relationship with colour, and an unexpected failure experience with Finnish clay. The study discusses the process of pushing through the internalised norms of working with clay, recognising self-established boundaries on creative identity; and it analyses colours’ impact on different levels throughout the project. Through a series of material and colour experiments with Finnish clay and altering the usual production methods of ceramic-making, this research work explores a wider spectrum of this material’s character and potential; while it also challenges the maker to critically reflect both on her own creative identity, and the concepts such as normal/weird, perfection, and failure, among others. The name Aesthetics of Chaos refers to the chaotic state of conducting a personally challenging research that deliberately forces the author to go out of the comfort zone and expand tolerances; and the molten, unruly, unapologetically free high-fired end results of the Finnish clay. This research work utilises various methods from practice-based design research and experimental material research fields. Making, documenting and reflecting on making itself goes hand in hand to gain a better understanding on intuitive reflexes of the maker, and acts an important source of insight for the research. Apart from making, the literature review is also utilised as an integral part of the process. It provides a theoretical structure for understanding, analysing and contextualising some of the concepts involved with and aroused from the making, such as one’s own creative identity, strong emotional influence and repulsion from certain colours, doubts, material agency and control. The research has a critical perspective on maker’s own pre-set standards and perceptions, and by engaging in a dialogue with
- Published
- 2021
18. Moderate prenatal ethanol exposure leads to attention deficits in both male and female rats
- Author
-
Wang, Ruixiang, primary, Martin, Connor D., additional, Lei, Anna L., additional, Hausknecht, Kathryn A., additional, Ishiwari, Keita, additional, Oubraim, Saida, additional, Wang, An‐Li, additional, Richards, Jerry B., additional, Haj‐Dahmane, Samir, additional, and Shen, Roh‐Yu, additional
- Published
- 2021
- Full Text
- View/download PDF
19. Animali in città.
- Author
-
Imbroglini, Cristina and Lei, Anna
- Abstract
Copyright of Ri-vista: Ricerche per la progettazione del paesaggio is the property of Firenze University Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
- View/download PDF
20. Prenatal ethanol exposure impairs sensory processing and habituation to visual stimuli, effects normalized by enrichment of postnatal environmental.
- Author
-
Wang, Ruixiang, Martin, Connor D., Lei, Anna L., Hausknecht, Kathryn A., Turk, Marisa, Micov, Veronika, Kwarteng, Francis, Ishiwari, Keita, Oubraim, Saida, Wang, An‐Li, Richards, Jerry B., Haj‐Dahmane, Samir, and Shen, Roh‐Yu
- Subjects
CHILD development deviations -- Risk factors ,LIGHTING ,SENSORIMOTOR integration ,ANALYSIS of variance ,SENSORY disorders ,ANIMAL experimentation ,GESTATIONAL age ,HEALTH outcome assessment ,PRENATAL exposure delayed effects ,RATS ,VISUAL perception ,DESCRIPTIVE statistics ,ETHANOL ,STATISTICAL sampling ,DATA analysis software ,FETAL alcohol syndrome ,DISEASE risk factors - Abstract
Background: Individuals with fetal alcohol spectrum disorders (FASD) often show processing deficits in all sensory modalities. Using an operant light reinforcement model, we tested whether prenatal ethanol exposure (PE) alters operant responding to elicit a contingent sensory stimulus—light onset (turning on the light) and habituation to this behavior in rats. We also explored whether postnatal environmental enrichment could ameliorate PE‐induced deficits. Methods: Pregnant Sprague Dawley rats were gavaged twice/day with 0 or 3 g/kg/treatment ethanol (15% w/v) during gestational days 8–20, mimicking second‐trimester heavy PE in humans. The offspring were reared in a standard housing condition or an enriched condition. Adult male and female offspring underwent an operant light reinforcement experiment with either a short‐access or a long‐access procedure. A dishabituation test was also conducted to characterize the habituation process. Results: In the short‐access procedure, PE led to increased operant responding to the contingent light onset in both sexes reared in the standard housing condition. Such an effect was not observed in rats reared in enriched conditions due to an overall decrease in responding. Moreover, rats reared in enriched conditions showed greater short‐term habituation. In the long access procedure, PE rats showed increased responding and impaired long‐term habituation. The long‐access procedure facilitated both short‐term and long‐term habituation in control and PE rats. Conclusion: Prenatal ethanol exposure increases responding to contingent light onset and impairs the long‐term habituation process. The PE‐induced deficits were ameliorated by rearing in the enriched environment and increasing the duration and frequency of exposure to light onset. The PE‐induced effects are like increased sensation‐seeking, a subtype of sensory‐processing deficit that is often observed in individuals with FASD. Our findings could inform a suitable animal model for investigating the underlying mechanisms and possible intervention strategies for sensory deficits in FASD. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
21. Additional file 15 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 15: Appendix [49–52].
- Published
- 2020
- Full Text
- View/download PDF
22. Additional file 7 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 7: Figure S7. Peptide array immune age is distinct from cytokine derived immune age. (A,B) Correlation between cytokine marker quantification by Luminex assay (y-axis, log-scale) and age (A) and BMI (B). (C) Peptide array prediction of age is not improved by cytokine data; however, prediction of BMI is significantly increased when including cytokine data. Graphs show on the y-axis, the relationship between peptide array signals (row a), cytokines (row b), and combinations of peptide array and cytokines (rows c and d) and on the x-axis, a combination function of chronological age and BMI that roughly approximates health (column i), chronological age (column ii) and BMI (column iii), as proxies for immune health. Row (c) trained on example matrix where peptide array and cytokine data were concatenated, whereas row (d) trained on matrix where only score derived from peptide array data was concatenated to cytokine data. Cytokine data was transformed by log10(x + 1) to make linear regression variance more homoscedastic. In this context, “concatenation” refers to combining two matrices (organized as donors as rows and measurements in columns) by adjoining column-wise after matching rows by donor. (D) Chronological age prediction by peptide array (y-axis) and cytokine levels (x-axis) finds that markers of humoral and innate immunity have related, but independent prediction of chronological age.
- Published
- 2020
- Full Text
- View/download PDF
23. Additional file 8 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 8: Figure S8. Additional control experiments suggest that age-associated antibody-peptide binding is driven by direct IgG binding. (A) Original image associated with Fig. 4b. In addition to filter columns, we also demonstrated IgG separation with Melon Gel purification; however, the Melon Gel purification assay reagents disrupted IgG-peptide array binding in the recombined fractions (the positive control) even though it produced superior IgG purification. It was thus excluded from downstream analysis. (B) The age-associated probes are not bound by anti-IgG secondary antibody, which is used for detecting IgG bound to peptides. Generic stickiness or peptides with similarity to IgG-Fc (which can partially bind secondary-antibody directly; y-axis) have minimal correlation with on age-association (x-axis). Each data point is a probe. (C-D) Interfering substances found in varying abundances in serum have limited impact on age-associated peptide probes. Serum from 4 healthy donors with and without an interfering substance was assayed by peptide microarray. Triglycerides, rheumatoid factor (RF), conjugated bilirubin, human anti-mouse antibody (HAMA), hemoglobin, and unconjugated bilirubin at a single high concentration (Methods). (C) The log10 ratio of serum with and without interfering substance (y-axis) is compared to the log10 ratio of serum from older (> 60 years) and younger ( 0.25 and RF log10 ratio > 0.05; other cutoffs provided similar values). Pearson’s and Spearman correlation were not near significance, r = 0.06, rho = 0.03 (p 30) vs lower BMI (
- Published
- 2020
- Full Text
- View/download PDF
24. Additional file 2 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 2: Figure S2. Peptide array fluorescent intensity is highly reproducible across reagent lots, independent cohorts, and assay batch. (A) To estimate reagent lot and assay batch impact, 66 donors from the Discovery Cohort were selected for repeat assay using arrays from an independent manufacturing synthesis batch. The initial quantification (x-axis) is shown for each probe (dot) for all donors (single scatter plot) compared to re-assay on an independent reagent lot (y-axis). Values shown are normalized intra-array by median and inter-array by probe mean. This normalization decreases artifactual correlation as a result of probe absolute quantification, which tends to be similar in many conditions. Thus, each scatter plot shows the donor-specific residuals, which are highly correlated in the initial and repeated quantification. (B,C) Age (B) and BMI (C) statistics are reproducible across multiple independent cohorts. Age is highly reproducible with nearly exact same large effect size for each probe across cohorts. BMI is reproducible, but with smaller overall effect size and increased variation in BMI log10 fold change. Axes show average log10 ratio and each data point is a peptide probe on the array. (D) Probe correlations and association with age are reproducible across reagent lot and assay batch. Donors are the 66 selected for technical replication (described in main text), probes are same as shown in main-text heatmap, axis clustering uses new technical replicate data using independent reagent and array synthesis lots.
- Published
- 2020
- Full Text
- View/download PDF
25. Additional file 3 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 3: Figure S3. Peptide probe motifs that are associated with ageing and BMI. (A) Peptide sequences that are associated with age, scatter plot same as in Fig. 1d. (B) Peptide sequences that are associated with BMI. (C) Sequence motifs in peptide probes associated with BMI. Probes associated with BMI typically have N-terminus glutamic acid (N-glutamic acid). Motif information content (bits, y-axis) is shown for each position (x-axis). (D) The N-terminus di-serine and glutamic acid motifs are present in nearly all age- and BMI-associated probes, respectively, whereas these motifs are much less common across non-age and non-BMI associated probes. Fraction of probes (y-axis) with specified motifs (color legend) are shown for age, BMI, and all other probes (x-axis). (E) The presence of N-terminus glutamic acid (E) residues is associated with BMI-correlated probes. A single E residue is comparable in significance to multiple E residues. The presence of glutamic acid at the N-terminus is significantly associated with BMI, whereas any other position in the peptide probe has limited association with BMI. (F) The di-serine score is similar across array formats. Donor samples were assayed on standard sized arrays (131 k probes, x-axis) and large-format arrays (3366 k probes y-axis) to find that the presence of N-terminus di-serine motif conferred similar association with age (axis values). Each data point represents a single donor. (G) On the 3366 k array format with both acetyl capped and uncapped probes (351 k array format, see Methods), age-associated probes are N-terminus acetylated capped. Each data point shows probe count (y-axis) for a single cutoff value to consider a probe to be age associated (x-axis). (H) Donor samples were assayed on arrays with 100% acetylated probes (x-axis) and arrays where only a fraction of probes where acetylated (y-axis). Probes that are acetylated in the split array (red) had higher association with age than those probes uncapped on the split array (black). This intra-array experimental design controls for potential inter-array confounders. Each data point represents a single probe and axes are average values across donors of specified age groups.
- Published
- 2020
- Full Text
- View/download PDF
26. Additional file 5 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 5: Figure S5. The regression model and residuals are consistent across multiple training and verification cohorts and potential confounding variables (array synthesis, ethnicity, sample collection site, and BMI). (A) The age regression residuals are consistent across multiple sub-training sets. The Training Cohort and Verification Cohorts were merged into a single large cohort (N = 1675). Two training sets were created, each of size 698, which left 279 samples as a holdout set. An elastic net regression model was trained on each of the training sets and then residuals were calculated on the holdout set of 279 samples (data points) using each of the models (axes). High correlation between model residuals suggests a low variance-error term, which is consistent with residuals potentially being biologically relevant. Result is representative of 100 simulated training set splits. (B) Age regression residuals (axes) are correlated when samples are assayed on different array formats, different training sets, and different algorithm parameters (subplots). Each dot is a single donor assayed for a single permutation of array type, training set, and algorithm parameters. Not all samples were assayed on all permutations of array type. Values on x- and y-axes are residuals, which normalize out the default transitive correlation of all models being correlated to chronological age. (C) Regression model yields similar results across samples binned by ethnicity and sample collection site. Each dot is single donor and each line shows regression predictions on grouped donors. Chronological age (x-axis) and prediction of age based on peptide array regression model (y-axis) are shown. Legend shows correlation coefficient, regression slope and intercept, and number of samples in a given bin. Data shown is regression model learned on Training Cohort and applied to the Verification Cohort. (D) The intercept (shift) and slope (interacting) terms associated with BMI, ethnicity, and collection state are not statistically significant. One-way ANOVA test statistics are shown in the Table. (E) Age-associated antibody-peptide binding to di-serine N-terminus peptides is highly consistent in a long-term study. A total of N = 16 donors consented to regular blood draws for > 1 yr. Donors with > 5 samples over > 1 yr (N = 13) had consistent age-regression values over this time period. Data shown for all donors (lines).
- Published
- 2020
- Full Text
- View/download PDF
27. Progetto di paesaggio e interazione con le altre specie viventi
- Author
-
Imbroglini, Cristina, Caravaggi, Lucina, and Lei, Anna
- Subjects
living structures ,landscape design project ,complexity ,natural resources - Abstract
Landscape design has always been considered a cultural crossroad, an open reference, a welcoming and available space of interpretation, but this does not equate to considering it a field of undetermined meanings nor a set of images difficult to recognize. Complexity, intended as aptitude to distinguish but not to separate , can be considered a genetic feature of the landscape design project since its modern ‘origins’, but often it is misunderstood for indeterminacy. Univocal terms, those terms apparently clear, frequently used to ‘confine’ the landscape design project (green areas, urban, suburban, natural, anthropic landscapes, etc., borgesian lists which will never get to clear doubts about ‘attributions’) reveal the crisis of ideas long worn by time and simplifications, only able to flatten every possibility of interpretation and start the usual nominalist debate, aimed mostly at (very complicated) disciplinary attributions. The hypothesis discussed in this article is that the landscape design project is instead clearly interpretable. The field of meanings to which the article will refer to is ascribable to the representation and design experimentation of relations with the natural world. These relations are obviously very different one from the other, historically defined, but related by the same research of spatial interaction between human communities, contexts of life and ‘other’ living species. Landscape designers have a common vision capable of looking at living species as traveling companions on the path to the project and not as mere ‘tools’, whether the individual experience of landscape designers is related to the design of gardens and parks, or comes from the study of layered landscapes which must not be wasted, or is oriented towards landscapes damaged by use and pollution, or is on projects of poetic comment or radical regeneration of degraded soils, whether it is referred to ecological adaptability or collective health, to social dialogue or the re-appropriation of abandonedspaces. Until a few years ago this central role was difficult to affirm, and landscape designerswere consideredmarginalfigures, exponents of a soft world, detached from real dynamics. The landscape design project instead, far from being accessory and decorative, is a hard project, and necessary, today more than ever. In the words of Kristine Hill: “People often think of landscape as something ephemeral or something at small scale, but landscape is massive and muscular and strong” (Hill 2012, p. 49). To argue more clearly about the peculiarities of the contemporary landscape design project, it may be useful to briefly outline some distinguishing features in other moments of history., Ri-Vista. Research for landscape architecture, Vol. 18 No. 1 (2020): Exploring Landscapes of Research
- Published
- 2020
28. Additional file 4 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 4: Figure S4. Illustration of how age and BMI can be used as proxies for immunosenescence, where regression residuals are values of interest. Hypothetical data are shown, emphasizing residuals with respect to a learned regression line. Dots are hypothetical donors (this is not real data) that have been labeled with an Immune Wellness proxy (x-axis, proxies selected are age and BMI) from which an immune age (y-axis) was learned. Three hypothetical donors are highlighted and their signed residuals are plotted on the right with interpretations of good, average, and poor immune wellness.
- Published
- 2020
- Full Text
- View/download PDF
29. Additional file 6 of Age-associated changes in the circulating human antibody repertoire are upregulated in autoimmunity
- Author
-
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, and Preveen Ramamoorthy
- Abstract
Additional file 6: Figure S6. Parameter selection and impact on accuracy and analytic characteristics of the regression model. (A) Regression accuracy and stability metrics (rows) are impacted by parameters alpha (columns) and lambda (x-axis). While accuracy (quantified by Pearson’s correlation r) is similar across many parameters, residual correlation and MSE can vary substantially. We select α = 0.01 and λ = 1 based on this analysis. Note that the residual correlation plot is undefined (currently shown as y = 1) when number of features in model is zero. Empty plots showing “number of features” on y-axis indicate that even for high λ = 10, we found > 1500 features included in model. (B) As training set size increases (x-axis) the learned model has improved accuracy as measured by Pearson’s correlation with chronological age (y-axis). Each training set is simulated 10 times from all 1675 samples using a holdout set to test for correlation with chronological age.
- Published
- 2020
- Full Text
- View/download PDF
30. Translation. Notes on colour and migration
- Author
-
van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, muo, Mäkelä, Maarit, Gurrola Alvarez, Jose, van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, muo, Mäkelä, Maarit, and Gurrola Alvarez, Jose
- Abstract
TRANSLATIONS is a study on colour in the context of migration. This research work takes as a starting point the author’s personal journey of leaving his home country of Mexico to settle in Finland. The study discusses the process of integration and identity building through the analysis of different aspects in the concept of colour, departing from the fact that the phenomena of colour and colour perception are on the one hand deeply individual, and yet highly informed by context and social constructs. Through a series of pieces, colour tests and interventions this research work explores the relation we build with colour and how it transforms when moving from point A to point B. Following the same line, the term translation is used in its different meanings to describe the process of transformation in words, objects and colour perception, creating a simile with the process of migration. The artistic research is documented in two chapters. In the first, a series of traditional Mexican wooden crafts are selected under the criteria of colour to be dissected and intervened in their materiality and appearance. The results of contextualising and decontextualising these objects are then discussed by the author to reflect on the object’s provenance, identity and practical function. In the second chapter, a technique for colouring wood is developed using pigments and several media to conduct a series of colour tests. A group of coloured wooden pieces are created using the act of colour testing itself as a method to translate different colour experiences deeply embedded in the author’s mind; experiences subjected to the translation from point A to point B. The conceptualisation of the outcomes of this artistic research on colour and colour perception becomes the ground to discuss different complexities of migration and the process of integration through the author’s own experience.
- Published
- 2020
31. Weaving landscapes
- Author
-
van der Lei , Anna-Marie, Savola, Kaisu, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Lohmann, Julia, Solis Escalera, Mariana, van der Lei , Anna-Marie, Savola, Kaisu, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Lohmann, Julia, and Solis Escalera, Mariana
- Abstract
“Weaving Landscapes” is a research through design thesis that explores the relationship between craft and design, by using concepts from the material culture, with(in) the landscape. Landscape in this thesis refers to the environment(s) in which humans and nonhumans interact. Meanwhile, material culture is the concept that brings together activities from human society and natural ecosystems (natural culture) into one culture. Furthermore, this thesis examines the collaboration that exists between craft and design, which is studied on the notions of preservation and revitalization. Craft is understood as the practice of traditional techniques, while design is used as the perspective and tool to question, think, understand and develop concepts. The relationship between these two practices, craft and design, is inquired through the knowledge in materiality, in making and in the society. The context of the thesis is situated in Finland, in the relation that birch bark weaving technique has with the natural environment and with the society. This topic was chosen because the interest in this traditional craft in Finland seems to have decreased along with its makers, whether they are professional craftspeople or hobbyists. This thesis argues that birch bark weaving should be reconsidered because of the concepts and values that it has within the Finnish landscape. The context is studied by examining how craft values can be regenerated and preserved while revitalizing craft and design practices. As part of my research, I have traveled to mid-Finland to learn and research the birch bark weaving technique from a professional craftsperson. The trip along with the theory have inspired me to create the project “A Dialogue with Birch”, a collection of explorative artefacts. These artefacts consist on canvases that express the outcome of a conversation between my hands and the material (birch bark). Finally, with this thesis I want to analyze and empathize with the connections that
- Published
- 2020
32. Pigments of microorganisms
- Author
-
van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Kääriäinen, Pirjo, Juuri, Eveliina, van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Kääriäinen, Pirjo, and Juuri, Eveliina
- Abstract
Colors are something that humans have always been fascinated by but impacts of different colorants have caused concern about health and environmental issues. Now more sustainable alternatives interest many industries since the demand for greener products has increased. To be able to answer these environmental challenges, biotechnology can offer new types of tools for design that are enabling co-working with living organisms as a source for new colors and material alternatives. This research project is executed in collaboration with professionals from biochemistry and with living organisms. Research seeks after inherent natural colorants that are produced by microorganisms, bacteria and fungi. Research focuses on exploring the challenges of isolating the pigment out from the microorganisms and also considering the ethical aspects of working with living. This research introduces a detailed description of a working procedure that was implemented with bacteria Serratia marcescens that is able to produce pigment called prodigiosin. In addition to this, working with another microorganism, fungi, is presented as a case study. Both of these projects focused on finding color, growing and isolating of the color from the microorganisms. To be able to tell more about the characteristics of the pigment, experimental color and material research was implemented with prodigiosin pigment that provided promising signs as a colorant. These future colorants can offer us more environmental friendly alternatives from a natural source. Colorants that are produced by microorganisms still require more research to be done of their environmental impacts and benefits but according to the results from this research these colorants already showed potential to be scaled and further developed., Värit ovat aina kiehtoneet ihmisiä, mutta erilaisten väriaineiden vaikutukset ovat jo pidemmän aikaa herättäneet keskustelua erilaisten terveys- ja ympäristöhaittojen vuoksi. Uudet ympäristöystävällisemmät vaihtoehdot kiinnostavat nyt monien eri alojen yrityksiä kuluttajien kasvavan vaatimuksen myötä. Bioteknologia voi tarjota muotoilulle uusia välineitä näiden haasteiden ratkaisuun mahdollistaen yhteistyön elävien organismien kanssa, jotka kykenevät tuottamaan erilaisia värejä ja materiaaleja. Tämä tutkimusprojekti on toteutettu yhteistyössä biokemian ammattilaisten sekä elävien mikro-organismien kanssa. Tutkimuksessa tavoitteena on löytää luonnollisia väriaineita, jotka voidaan tuottaa bakteerien ja sienien avulla. Tutkimus keskittyy tarkastelemaan, onko näistä mikro-organismeista mahdollista eristää kiinnostavia väripigmenttejä ja mitkä ovat haasteet työn ympärillä samalla arvioiden työn eettisiä näkökulmia. Työ esittelee yksityiskohtaisen kuvauksen työskentelymenetelmästä, jota hyödynnettiin Serratia marcescens bakteerin kanssa, joka kykenee tuottamaan prodigiosin pigmenttiä. Tämän lisäksi työ esittelee tapaustutkimuksen, jossa väriä pyrittiin tuottamaan sienten myseelin avulla. Molemmat projektit keskittyvät värin löytämiseen, kasvattamiseen ja väriaineen eristämiseen mikro-organismeista. Jotta väripigmentin luonteesta voitaisiin kertoa enemmän, toteutettiin prodigiosin pigmentin avulla kokeellinen väri- ja materiaalitutkimus, joka tarjosi lupaavia merkkejä väriaineena. Tutkimuksen tuloksena saatiin tuotettua väriaineita sekä väripigmentti onnistuneesti. Nämä tulevaisuuden väriaineet voivat tarjota ympäristöystävällisen vaihtoehdon kirkkaiden värien saamiseksi, samalla sisältäen terveydelle hyödyllisiä ominaisuuksia. Mikro-organismeilla tuotetut väriaineet vaativat vielä lisätutkimusta niiden todellisista ympäristövaikutuksista ja -hyödyistä, mutta jo tämän tutkimuksen tulosten perusteella voitiin huomata, että väriaineissa on potentiaalia skaalattaviksi ja jat
- Published
- 2020
33. New urban forests in layering landscapes
- Author
-
Caravaggi, Lucina, Imbroglini, Cristina, Lei, Anna, and Lentini, Manuel
- Subjects
rome ,urban forst, rome, environmental and landascpe evolutions and layering ,urban forst ,environmental and landascpe evolutions and layering - Published
- 2019
34. Prenatal Ethanol Exposure Leads to Attention Deficits in Both Male and Female Rats
- Author
-
Wang, Ruixiang, primary, Martin, Connor D., additional, Lei, Anna L., additional, Hausknecht, Kathryn A., additional, Ishiwari, Keita, additional, Richards, Jerry B., additional, Haj-Dahmane, Samir, additional, and Shen, Roh-Yu, additional
- Published
- 2020
- Full Text
- View/download PDF
35. Moving bodies
- Author
-
van der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, Cortés Reina, Camilo, van der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, and Cortés Reina, Camilo
- Abstract
Moving Bodies is a maker’s personal narrative. It illustrates a journey towards finding one’s voice as a designer through a material experimentation process that resulted in the making of The Moving Bodies. The project begins with self doubt and and a question: “Who am I?”, and continues with the evolution of the author’s thoughts as a maker leading to the creation of his sculpture series Moving Bodies. A material experimentation process in ceramics is narrated through the analysis and self-reflections of the author’s own practice. By studying himself and his own mind, he explores his values and through an hands approach he is able to deeply analyse his intuition, a key point to understand creative decisions. Learning by doing, letting go of set guidelines and prioritising the value of a maker’s process over planning and clear cut results are defining points of this body of work. The narrative is far from linear, conscious design decisions are showcased with a no-nonsense attitude and most importantly, a detailed analysis is made on where those decisions stem from. Moving Bodies does not draw clear lines between experimental ceramic samples, the very first material development tests and the final outcomes. The process of creating or stumbling upon a new ceramic material, followed by trying to master and understand the material results in an extremely hands-on diary focused both on personal thoughts and technical information. Material and maker co-creation as a method is introduced, the moving bodies, free flowing ceramic bodies, is the main collaborator to the maker himself. Every test piece or sculpture is essentially the maker creating a playground for the material to free-flow. Material co-creation is a back and forth dialogue between the maker and the material he is trying to guide, form and understand. The Moving Bodies are created through many unorthodox methods of crafting ceramics and setting up molding and forming techniques for the material in the ceramic
- Published
- 2019
36. transcendence
- Author
-
van der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Lohmann, Julia, West, Sini, van der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Lohmann, Julia, and West, Sini
- Abstract
A state of chaos conflicts with reason and discipline, repressing order and precision. Entropy can be organised into logic only with information. Disorder arranged by knowledge increases the opportunity for definition as it reveals measurable differences. What can be methodologically measured, known, justified and thus argued as knowledge, is interpreted by our sensorial system. However, our perceptional capabilities are limited and affecting the spectrum of collectible data, thus restricting our notion of reality. Preserving knowledge through time, tradition is mediated by language, culture and interaction. Tradition consists of propositional knowledge, a truth that relies on empirical observation and experience. Therefore, the provability of the external reality is tightly linked to our subjective experientiality. Knowledge and information of a contrary kind, existing remote from the rational and measurable, is in this research defined as transcendental. Transcendence is a phenomenon or an assortment of events occurring hidden from our bare senses, beyond the realm of our consciousness. This thesis is an exploration through art, design and philosophy, reflecting the topic of combining experimental material design with VR technology on the discourse of new materialities in the digital age and research on current knowledge on the processes of perception and presence. The project unravels the path to a transcendental level of information, which is collected and used as the worked material. The design and construction processes of a virtual environment extend the conventional studio facilities and practice, and present affordances, new possible directions and different perspectives for a material experimentation process. Virtual reality enables the observation of glass in an alternative context where its transformed phase, interpreted by complex systems, reveal another dimension of its complete objecthood that can be perceived through designated devices. Through recip, Kaaos on järjen ja kurinalaisuuden vastakkainen tila, joka pidättelee järjestystä ja tarkkuutta. Ainoastaan informaation avulla entropian on mahdollista järjestyä loogiseen muotoon. Tiedon avulla järjestetty sekasorto paljastaa mitattavia eroavaisuuksia tuoden näin esiin mahdollisuuden määrittelylle. Kuitenkin kaikki, mikä voidaan metodologisen mittaamisen, perustelun ja todentamisen myötä vahvistaa tiedoksi, on lopulta aistijärjestelmämme tulkitsemaa. Havaintokykymme on rajoittunutta, ja tämä ominaisuus vaikuttaa kaiken kerättävissä olevan tiedon kirjoon, vaikuttaen käsitykseemme todellisuudesta. Traditio säilyttää tietoa ajan kuluessa välittyen eteenpäin kielen, kulttuurin ja interaktion kautta. Traditio on propositionaalista tietoa, joka nojaa empiirisiin havaintoihin ja kokemuksiin linkittäen näin ulkoisen todellisuuden todistettavuuden vahvasti subjektiiviseen kokemuksellisuuteemme. Vastakohtainen, rationaalisuuden ja mitattavuuden ulkopuolella oleva tieto on tässä tutkimuksessa määritelty transsendentaaliseksi. Transsendenssi on ilmiö tai kokoelma tapahtumia, jotka ilmenevät tietoisuutemme ulottumattomissa, biologisen aistikykymme ulkopuolella. Tämä opinnäytetyö on tutkimusmatka taiteen, suunnittelun ja filosofian alueille. Se reflektoi kokeellisen materiaalisuunnittelun ja VR-teknologian yhdistämistä digitalisoituneella aikakaudella ilmenneisiin uusiin materiaalisuuden käsityksiin sekä tämänhetkiseen tietoon ihmisen havainnon ja läsnäolon prosesseista. Projekti selvittää tietä transsendentaaliselle tasolle, jossa kerättyä ja tallennettua informaatiota käytetään työstettävänä materiaalina. Virtuaalisen ympäristön suunnittelu ja rakentaminen laajentaa perinteisiä työskentely-ympäristöjä ja -käytäntöjä sekä tuottaa affordansseja, uusia mahdollisia suuntia ja erilaisia näkökulmia materiaalikokeiluille. Virtuaalitodellisuus mahdollistaa lasin tarkastelun vaihtoehtoisessa kontekstissa, jossa fyysisen objektin olomuoto ja kokonaisvaltainen esineellisyys tulkitaan uu
- Published
- 2019
37. Human element
- Author
-
Van Der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Mäkelä, Maarit, Velkoff, Collin, Van Der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Mäkelä, Maarit, and Velkoff, Collin
- Published
- 2018
38. The Sweater Work / Shop
- Author
-
van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, Lachner, Valentina, van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, and Lachner, Valentina
- Abstract
The Sweater Work / Shop is a project focusing on the utilization of DIY, crafts and making techniques in a design context. Setting out to develop a small-scale system, this Thesis explores the possibilities of working with textile waste in new and innovative ways through hands-on making while searching for an alternative to mass consumption. Theoretical research provides relevant and contemporary knowledge about the key areas of DIY, crafts and making, as well as their suggested benefits for the environment, society, individual wellbeing and the human-object relationship. Added to this, an extensive practical research provides deeper insights into these themes, related businesses and local projects, together with applied knowledge about the DIY, crafts and making process in a series of experiments with techniques and materials. An in-depth analysis summarizes the most significant problems and opportunities learned by application of the previously mentioned research methods, resulting in the formulation of a design brief for the practical prototype. The prototype itself is a small mobile kiosk to show, make and sell. It visualizes the process of un-knitting old garments, making recycled yarns and then knitting new products from these yarns. It serves both as a workstation and a small shop, therefore the title of this Thesis: The Sweater Work / Shop. This prototype is combined with an alternative pricing system, offering customized products for a lower price, and thereby creating value through engagement of the customer and the story of the making process instead of monetary investments. In the end, a real-life trial proved, that customized products offer a good balance of involvement; allowing even those who don’t want to craft, DIY or make to participate and benefit from some of the positive aspects of DIY, crafts and making.
- Published
- 2018
39. Thin cuts
- Author
-
van der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Kokkonen, Ville, Horvath, Zsuzsanna, van der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Kokkonen, Ville, and Horvath, Zsuzsanna
- Abstract
Paper cutting has been a well-known and developed form of art for centuries, traditionally performed in China and Japan since the invention of paper. It is still popular today with even newer ways of cutting emerging from the use of modern technologies. This thesis builds on current and traditional paper cutting methods to develop a new type of material manipulation using digital fabrication. The focus of this work is on practice-based material research of paper, cardboard, wood pulp, plywood and metal, and explorative prototyping using cutting and bending techniques to reshape the thin sheets into three-dimensional objects, aiming to widen the boundaries of material use. The research is based on self-developed cutting patterns and is documented by photos and videos of the process and outcome. After the first tests are conducted on the selected materials, they are analysed and evaluated based on the material characteristics after the cutting process, the cutting patterns and resulting shapes, durability as well as possible functions. After careful consideration, one particular cutting pattern has been selected to be developed into the final outcome of this thesis, a pendant lamp. The design process is documented by sketches, computer models, photographs of mock-ups and prototypes. After several sets of prototypes, Sila pendant lamp is been refined to its current shape incorporating the feedback from the public and professionals in three consecutive exhibitions, where it was presented.
- Published
- 2018
40. Delfuksi tulemisesta
- Author
-
Tervo, Juuso, Van Der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Muotoilun laitos, Department of Design, Mäkelä, Maarit, Yau, Man, Tervo, Juuso, Van Der Lei, Anna, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Muotoilun laitos, Department of Design, Mäkelä, Maarit, and Yau, Man
- Abstract
Opinnäytetyö Delfuksi Tulemisesta koostuu taiteellisesta työstä sekä kirjallisesta osiosta. Taiteellinen työ on veistoskokonaisuus, joka esiteltiin Galleria Huudossa 5.-20.8.2017. Näistä veistoksista esittelen tarkemmin kaksi kokonaisuutta, keraamisen Sea-installaation ja puusta koostuvan Delfu-veistoksen. Kirjallisessa osiossa kuvailen kyseisten veistosten työprosessia, keskittyen taiteellisen ajatuksen kulkuun. Kuvailen kolmesta eri näkökulmasta miten työn lähtökohta määrittelee valintoja ja valitsematta jättämisiä veistoksen työprosessissa. Opinnäytetyön keskiössä on teoksen ja tekemisen suhde siihen, mitä se tarkastelee. Reflektoin omaa taiteellista työskentelyprosessia suhteessa lähdekirjallisuuteen, Max Horkheimerin ja Theodor W. Adornon kirjaan Valistuksen Dialektiikka: Filosofisia sirpaleita, keskittyen erityisesti lukuun Kulttuuriteollisuus: Valistus massapetoksena (2008). Kyseisen luvun tulkinnan kautta syntyy työn lähtökohta ja koko taiteellista produktiota ohjaava metodi: Intuitio muutetaan mittaamiseksi, näkemys järjestetään systemaattiseksi ja tämä systeemi imitoi sitä muovista ympäristöä, jossa huomaamatta elämme. Metodista syntyy vieraannuttamisen efekti, oman tekijyyden piilottaminen, joka näkyy jokaisessa työvaiheessa näyttelyn ripustukseen asti. Käyn työskentelyprosessin läpi kolmen määrittelemäni termin avulla. Kappaleessa Käytäntö kuvailen miten valitsemani metodi näkyy studiolla tapahtuvassa työprosessissa. Keramiikan työstäminen tapahtuu keramiikan työtavoin, mutta kaikkea tekemistä ohjaa taiteellinen ajatus. Kohdassa Paljastaminen kerron kehollisen työskentelyn merkityksestä minulle, joka on oleellinen elementti metodia ajatellen. Vieraannuttamisen efekti, joka ohjaa työskentelyä, pyrkii peittämään kaikki kehollisen työskentelyn työvaiheet, joista syntyy minulle itselleni merkityksellinen tunne. Piilottamalla oman käden jäljen, piilotan samalla opitut tekniikat ja käytettävän materiaalin, työhön käytetyn ajan sekä studion, missä olen työstäny, The Master’s thesis Delfuksi Tulemisesta (eng. On becoming Delfu) consists of artistic work as well as a written section. The artistic work is a set of sculptures that were presented at Gallery Huuto during 5.-20.8.2017. I’ve selected two works, the ceramic installation Sea and the wooden sculpture Delfu, of which I speak of in depth. In the written section I describe the work process of these sculptures focusing on the artistic thought behind them. Using three different perspectives I describe how the work’s basis defines the choices of doing and not doing during the sculpture’s artistic process at the studio. In the center of the thesis is the relationship of the artwork and it’s artistic process towards that which it surveys. I reflect my own artistic work process in relation to the reference literature, Max Horkeimer’s and Theodor W. Adorno’s Valistuksen Dialektiikka: Filosofisia sirpaleita (eng. Dialectic of Enlightenment: Philosophical Fragments), especially focusing on the chapter Kulttuuriteollisuus: Valistus massapetoksena (eng. The Culture Industry: Enlightenment as Mass Deception). Through the interpretation of this chapter the basis for the work and also the method which guides the entire artistic production is born: Intuition is transformed into measuring, insight is arranged as systematic and this system imitates the plastic environment in which we live in without realizing it. Out of this method is born an effect of distancing, the hiding of authorship, which is seen in every stage of the working process. I go through the working process with three different perspectives I’ve defined as terms. In the chapter Käytäntö I describe how the method I’ve chosen is visible in the working process at the studio. Using ceramics as a material determines the specific techniques and requirements in the work process but all the work is guided by the artistic thought. In the second chapter Paljastaminen I speak of the meaningfulness of the physical work for me. It is
- Published
- 2018
41. How I learned to stop worrying and love the object
- Author
-
Van Der Lei, Anna, Song, Aamu, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, Melentjeff, Alli Roosa Sofia, Van Der Lei, Anna, Song, Aamu, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, and Melentjeff, Alli Roosa Sofia
- Abstract
The state of awareness that is possible to achieve today by being constantly engaging with devices of technology had left me deeply concerned, about everything. My concern about the future, in global as in personal spectrum, had affected diminishingly on my abilities to work in a field where the product of my labour was, to me, just an object. I feared that the crafts field I was graduating to as a ceramicist was an aging, dusty one and becoming obsolete in today’s society. This thesis aims to explore the meaningfulness of objects and the beautiful relationship between them and people through makers eyes. Through the production I am exploring my creative process and methods and trying to learn new ways to comprehend that process more, in order to find enjoyment in the making and in the product. I am reflecting my own and people’s relationship to pieces I’ve made, and pondering why we love certain objects. I am exploring the relevance of objects through theoretical literature studying people and their relationship to objects through ethnographical, sociological and philosophical look. I am also skimming through texts concerning creative process and making, and exploring how the relationship with objects is expressed by makers and people using them. I am also addressing the subject of the value of small scale production in order to clarify wether crafts industry today is obsolete or not. By also delving into my own creative process and exploring what methods I use to find inspiration I will go deeper into the process of creating objects, finding enjoyment in the process and finally, in the object., Tietoisuuden taso joka on mahdollista saavuttaa tänä päivänä tietoteknisten välineiden avulla oli tehnyt minusta huolestuneen, huolestuneen kaikesta. Huolestuneisuuteni tulevaisuudesta, globaalista sekä henkilökohtaisesta, oli johtanut kykenemättömyyteeni toimia omalla alallani, jossa tekemiseni tuotos oli minulle vain esine. Valmistuessani keraamikoksi ja käsityöläiseksi pelkäsin koko käsityöläisalan olevan vain pölyttynyt ja aikansa elänyt tässä yhteiskunnassa. Tämä opinnäytetyö pyrkii tutkimaan esineiden merkityksellisyyttä sekä niiden ja ihmisten välistä suhdetta, tekijän silmin. Produktion kautta tutkin omaa luovaa prosessiani ja työskentelymetodeja, tarkoituksenani oppia uusia tapoja ymmärtää prosessia ja siten löytää iloa esineestä ja niiden tekemisestä. Peilailen omaa ja ihmisten suhdetta valmistamiini esineisiin ja pohdin, miksi jotkin esineet meitä kiinnostavat. Tutkin esineiden merkityksellisyyttä teoreettisesti kirjallisuuden pohjalta jossa tutkitaan ihmisiä ja heidän suhdettaan esineisiin etnograafisten ja sosiologisten tutkimusten pohjalta sekä filosofiselta kannalta. Tutustun myös teksteihin koskien luovaa prosessia ja tekemistä, sekä miten käyttäjät ja tekijät ilmaisevat suhdettaan esineisiin. Otan myös esille pientuotantoesineiden merkityksen ja arvon selventääkseni onko käsityöläis-ala olemassa vai. Sukeltaessani omaan luovaan prosessiini ja tutustuessani käyttämiini työskentelymetodeihin inspiraation löytämiseksi pyrin myös näiden kautta löytämään iloa tekemisen prosessista ja lopulta itse esineestä.
- Published
- 2018
42. Empty house
- Author
-
Van der Lei, Anna-Marie, Fagerlund, Hilkka-Maija, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Kareoja, Pentti, Mussi Weidlich, Nathalia, Van der Lei, Anna-Marie, Fagerlund, Hilkka-Maija, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Kareoja, Pentti, and Mussi Weidlich, Nathalia
- Abstract
At the center of this work is an empty house and the urgency to keep memories safe. Our memories don’t exist in a vacuum, they are born from instances of reality. Buildings and fabrics, the spaces where we live our lives, the layers that involve our bodies, have the power to trigger memories. However, a memory is not a safely stored footage of reality. The act of remembering is entangled with imagination as we inadvertently seek for a coherent narrative. Familiar places have an important role in safeguarding memories, they are ever present as we carry out our lives and they change drastically in the circumstance of death. This MA thesis is a personal reflection on the relationship of memory, presence and place in the context of loss: a reflection that is carried simultaneously in writing as in weaving. It encompasses a research through the fields of Philosophy of Memory and Phenomenology, pulling a thread from the topic of memory and following it through connections to imagination, narrative, emotion and language, presence the immateriality of the dwelling space and the subjective dimension of time. The researched topics are interwoven with personal stories and with the making of a thin metallic fabric, embracing reflections on the meaning of the empty house while turning to the process of hand weaving to create a site specific artifact.
- Published
- 2018
43. Väriefektiä etsimässä. Ympäristöystävällistä rakenteellista väriä puusta - Yhteistyöprojekti materiaalitieteen ja muotoilun välillä
- Author
-
van der Lei, Anna, Beidler, Jaana, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Muotoilun laitos, Department of Design, Yau, Noora, van der Lei, Anna, Beidler, Jaana, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Muotoilun laitos, Department of Design, and Yau, Noora
- Abstract
Opinnäytetyö on kuvaus yhteistyöprojektista muotoilun ja materiaalitieteen välillä. Se tarkastelee muotoilijan roolia osana materiaalin kehitysprosessia. Tutkimuksessa kehitin nanoselluloosapohjaista rakenteellista väriä yhteistyössä Aalto-yliopiston kemian tekniikan korkeakoulun biotuotteiden ja biotekniikan laitoksen kanssa ja tutkin sen mahdollisia käyttösovelluksia. Opinnäytetyö on kertomus siitä, miten henkilökohtainen kiinnostukseni hohtavia värejä kohtaan kasvoi hiljalleen monialaiseksi tutkimus- ja kehittämistyöksi. Opinnäytetyön ensimmäisessä osassa keskitytään tutkimuksen taustoihin, omaan henkilökohtaiseen kiinnostukseeni kimaltavia ja hohtavia väriefektejä kohtaan, väriin ilmiönä ja rakenteellisen värin määritelmään. Rakenteellinen väri voidaan määritellä värinmuodostusmetodiksi. Se on luonnon tapa tuottaa hohtavia ja irisoivia väriefektejä esimerkiksi riikinkukkojen sulissa ja taivaansiipien eli Morpho-suvun perhosten siivissä. Ilmiö perustuu nanokokoisen rakenteen ja valon vuorovaikutukseen. Pyrin tutkimuksessani tuomaan ilmi rakenteellisen värin ja pigmenttivärien eroja. Esittelen eläinkunnassa esiintyvää rakenteellista väriä ja innovaatioita, joita tästä värinmuodostusmenetelmästä on jo kehitetty. Kimaltavat ja hohtavat värit ovat nouseva trendi mm. kosmetiikan, vaateteollisuuden ja huonekaluteollisuuden aloilla. Metallinhohtoisten ja irisoivien väriefektien toteutukseen liittyy kuitenkin ongelmia: Ne valmistetaan usein uusiutumattomia muovi- ja metallipohjaisia aineita käyttäen ja ovat siksi haitallisia ympäristölle. Nanoselluloosaa voidaan valmistaa puusta, jotka ovat luonnollinen, uusiutuva luonnonvara. Nanoselluloosasta tuotettava rakenteellinen väri voisi siis olla yksi mahdollisuus valmistaa kimaltavia efektivärejä ympäristöystävällisesti. Materiaali ei kuitenkaan ole vielä valmis kaupallisiin sovelluksiin, vaan sen hyödyntäminen ja käyttöönotto teollisuudessa edellyttää jatkoa muotoilijoiden ja materiaalitieteilijöiden yhteiselle kehitystyölle, This thesis is a description of a collaborative project between design and material science. It investigates the role of a designer as a part of the development process of a material. In the study, I developed nanocellulose-based structural color and researched potential practical applications for it, all in collaboration with the Department of Bioproducts and Biosystems at the Aalto University School of Chemical Engineering. This thesis is a story of how my personal interest in shiny colors slowly grew into interdisciplinary research and development work. In the first part of the thesis, I focus on the background of the research, my personal interest in glimmering and shiny color effects, color as a phenomenon, and the definition of structural color. Structural color can be defined as one of the mechanisms of color production. It is nature’s way of producing shiny, iridescent color effects, found for instance in peacock feathers and in the wings of the genus Morpho butterflies. The phenomenon is based on the interaction of light and nanostructure of the surface. In my research, I aim to bring out differences between structural color and pigment color. I will discuss structural color as it occurs in animals, and innovations that have already made use of this means of color production. Glimmering and shiny colors are a rising trend in e.g. the cosmetics, clothing, and furniture industries. However, production of metallic and iridescent color effects is problematic, as they are often made using non-renewable, plastic and metal-based ingredients, which are harmful to the environment. Nanocellulose can be extracted from wood, which is a natural, fully renewable resource. Thus, structural color made using nanocellulose could be an opportunity to produce glitter effects in an environmentally friendly way. The material is not ready for commercial applications yet, and further collaboration between designers and material science experts is required, until these industries c
- Published
- 2018
44. Agricolture urbanizzate per nuovi paesaggi metropolitani
- Author
-
Lei, Anna
- Subjects
Settore ICAR/15 - Architettura del Paesaggio ,architettura del paesaggio ,Europa 2020 ,innovazione sociale ,co-progettazione ,agricoltura urbana ,aree metropolitane - Published
- 2016
45. Paesaggi socialmente utili. Presentazione del libro
- Author
-
Lei, Anna and Tentori, Luca
- Published
- 2016
46. Elaborazione della matrice di valutazione della trasformabilità dei drosscape. Indicatori, moltiplicatori e interpretazione dei risultati
- Author
-
Secchi, Roberto, Alecci, Maurizio, Bruschi, Andrea, Guarini, Paola, Amore, Federica, Corsi, Andrea, Corvino, Enrica, Dedda, Martina, Ghazi, Elnaz, Iacovantuono, Armando, Lei, Anna, Massaro, Saverio, Morselli, CONCETTA ELISA CHIARA, Piccinno, TEODORA MARIA MATILDA, and Spirito, Gianpaola
- Subjects
drosscape ,rifiuti ,rigenerazione ,riuso ,riciclo - Published
- 2016
47. Strategie di recupero tra progetto e norma nel settore della nautica
- Author
-
Bruschi, Andrea, Guarini, Paola, Secchi, Roberto, Amore, Federica, Corsi, Andrea, Corvino, Enrica, Dedda, Martina, Ghazi, Elnaz, Iacovantuono, Armando, Lei, Anna, Massaro, Saverio, Morselli, Elisa, Piccinno, TEODORA MARIA MATILDA, and Spirito, Gianpaola
- Published
- 2016
48. Progetto di paesaggio e interazione con le altre specie viventi.
- Author
-
Caravaggi, Lucina, Imbroglini, Cristina, and Lei, Anna
- Abstract
Copyright of Ri-vista: Ricerche per la progettazione del paesaggio is the property of Firenze University Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
- Full Text
- View/download PDF
49. The Blue Moment
- Author
-
van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Muotoilun laitos, Department of Design, Salli, Timo, Maldonado Guisado, Laura, van der Lei, Anna-Marie, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Muotoilun laitos, Department of Design, Salli, Timo, and Maldonado Guisado, Laura
- Published
- 2017
50. Food waste stories: Ceramic experiments
- Author
-
van der Lei, Anna-Marie, Falin, Priska, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, Tolvanen, Irene, van der Lei, Anna-Marie, Falin, Priska, Taiteiden ja suunnittelun korkeakoulu, School of Arts, Design and Architecture, Department of Design, Muotoilun laitos, Mäkelä, Maarit, and Tolvanen, Irene
- Abstract
This thesis begins with making glazes for ceramics out of food waste from a local restaurant. The initial idea is to develop specific glaze recipies, but when the work developed the philosophical aspects became more important. The main reserach question is: what kind of a glaze type of finish with food waste can be created on ceramics? Later on the question arose that is there a poetic connection between the user and the objects created? In this thesis glaze as a concept is quite an open term meaning the different finishes created on ceramics with food waste. Later on in the process coffee waste and coffee powder were studied as a material on ceramics. Wood firing was chosen as a firing method for most of the pieces, which created especially interesting results. This project is divided into two parts, which will focus on the results from the point of view of aesthetics and test table settings to analyze the aesthetic qualities of the pieces. The aesthetics developed here are based on aesthetic theories found in literature combined with the maker’s background in ceramics. In the test pieces the food waste seems to have entered the pieces and they have become relics of the past meals. Some of the results remain art pieces and only intended for un-commercial use., Tämä lopputyö alkaa lasitteen valmistamisella keramiikalle ruokajätteistä, jotka on hankittu paikallisesta ravintolasta. Alkuperäinen idea oli kehittää tiettyjä lasitereseptejä, mutta projektin edetessä filosofiset kysymykset tulivat tärkeämmiksi. Päätutkimuskysymys on: millaista lasitteenomaista jälkeä voi ruokajäte luoda keramiikalle? Myöhemmin syntyi kysymys siitä, että onko käyttäjän ja luodun esineen välillä runollista yhteyttä? Tässä lopputyössä lasite konseptina on melko avoin termi ja tarkoittaa eri pintoja, joita on luotu keramiikalle ruokajätteen avulla. Myöhemmin prosessin aikana kahvijäte ja kahvijauhe olivat tutkimuksen kohteena materiaalina keramiikalle. Puupoltto valittiin polttomenetelmäksi suurimmalle osalle esineitä, joka loi erityisen kiinnostavia tuloksia. Tämä projekti jakautuu kahteen osaan, jotka keskittyvät tuloksiin estetiikan näkökulmasta ja testikattauksiin, joissa analysoidaan esineiden esteettisiä ominaisuuksia. Estetiikka, jota tässä on käsitelty pohjautuu kirjallisuudesta löydettyihin estetiikan teorioihin sekä tekijän itsensä taustaan keramiikassa. Ruokajäte on mennyt esineiden sisään ja ne ovat muuttuneet jäänteiksi menneistä aterioista. Jotkut tuloksista jäävät taide-esineiksi ja ovat tarkoitettu vain ei-kaupalliseen käyttöön.
- Published
- 2017
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.