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142 results on '"Lei, Anping"'

29. Additional file 1 of Water reuse and growth inhibition mechanisms for cultivation of microalga Euglena gracilis

Author

Wu, Mingcan, Du, Ming, Wu, Guimei, Lu, Feimiao, Li, Jing, Lei, Anping, Zhu, Hui, Hu, Zhangli, and Wang, Jiangxin

Abstract

Additional file 1: Fig. S1. Microalgae harvesting equipment. a, microalgae suspension; b, ultrafiltration membrane; c, pre-concentrated microalgae; d, reused water. Fig. S2. PCA analysis for groups EEG and IEG. IEG, EEG represent intracellular and extracellular metabolites, respectively. Fig. S3. OPLS-DA analysis for group EEG and IEG. IEG, EEG represent intracellular and extracellular metabolites, respectively. Fig. S4. Permutation test for group EEG and IEG. IEG, EEG represent intracellular and extracellular metabolites, respectively. Fig. S5. Heatmap of hierarchical clustering analysis for group EEG and IEG. IEG, EEG represent intracellular and extracellular metabolites, respectively; * represents the metabolite can be annotated in KEGG database.

Published
2021
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40. Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors

Author

Lei Anping, Chen Huan, Shen Guoming, Hu Zhangli, Chen Lei, and Wang Jiangxin

Subjects
Biofuel, Gene expression, Fatty acid synthesis, Green microalgae, Fuel, TP315-360, Biotechnology, TP248.13-248.65
Abstract

Abstract Background Biofuel has been the focus of intensive global research over the past few years. The development of 4th generation biofuel production (algae-to-biofuels) based on metabolic engineering of algae is still in its infancy, one of the main barriers is our lacking of understanding of microalgal growth, metabolism and biofuel production. Although fatty acid (FA) biosynthesis pathway genes have been all cloned and biosynthesis pathway was built up in some higher plants, the molecular mechanism for its regulation in microalgae is far away from elucidation. Results We cloned main key genes for FA biosynthesis in Haematococcus pluvialis, a green microalga as a potential biodiesel feedstock, and investigated the correlations between their expression alternation and FA composition and content detected by GC-MS under different stress treatments, such as nitrogen depletion, salinity, high or low temperature. Our results showed that high temperature, high salinity, and nitrogen depletion treatments played significant roles in promoting microalgal FA synthesis, while FA qualities were not changed much. Correlation analysis showed that acyl carrier protein (ACP), 3-ketoacyl-ACP-synthase (KAS), and acyl-ACP thioesterase (FATA) gene expression had significant correlations with monounsaturated FA (MUFA) synthesis and polyunsaturated FA (PUFA) synthesis. Conclusions We proposed that ACP, KAS, and FATA in H. pluvialis may play an important role in FA synthesis and may be rate limiting genes, which probably could be modified for the further study of metabolic engineering to improve microalgal biofuel quality and production.

Published
2012
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44. Effect of crude microcystin on the viable but non-culturable state of Aeromonas sobria in aquatic enviroment

Author

Li Shuangfei, Pan Gaoshan, Hu Zhangli, and Lei Anping

Subjects
chemistry.chemical_classification, biology, Natural water, Microcystin, Aquatic Science, biology.organism_classification, Pollution, Aeromonas sobria, Lake water, Microbiology, chemistry, Aquatic environment, polycyclic compounds, Earth and Planetary Sciences (miscellaneous), Molecular mechanism, Bacteria, Water Science and Technology
Abstract

Viable But Non-Culturable (VBNC) is a special state of bacteria for survival. It is a conventional state for bacteria in natural water body. Complicated environment factors may be the rational reason of the interchange. In this study, the effects of cyanobacterial media and crude microcystin on the VBNC state of A. sobria were conducted by incubating Aeromonas sobria into a sterile lake water. The results showed that some bacteria were in the VBNC state while others in culturable state; both cyanobacterial media and crude microcystin could improve the change of A sobria from VBNC state to culturable state. It revealed that microcystin might be an important environmental factor involved in the VBNC state transfering in aquatic environment. More research concerned about molecular mechanism was undergoing.

Published
2008

47. Study on Succession of a Mangrove Forest in Futian, Shenzhen Bay

Author

雷安平 Lei Anping, 陈里娥 Chen Li'e, 昝启杰 Zan Qijie, 周凯 Zhou Kai, 卢群 Lu Qun, 曾小康 Zeng Xiaokang, and 石俊慧 Shi Junhui

Subjects
Geography, Ecology, Forestry, Ecological succession, Mangrove, Bay, Ecology, Evolution, Behavior and Systematics
Published
2014

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