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Submitted by Edineia Teixeira (edineia.teixeira@unioeste.br) on 2017-11-27T18:31:17Z No. of bitstreams: 2 MARCELA LEITE2017.pdf: 2527004 bytes, checksum: 37310bebca45bcc15d56cfda1b505434 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Made available in DSpace on 2017-11-27T18:31:17Z (GMT). No. of bitstreams: 2 MARCELA LEITE2017.pdf: 2527004 bytes, checksum: 37310bebca45bcc15d56cfda1b505434 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-01-25 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Objective: To evaluate whether periodontal disease, through systemic inflammation, can potentiates the deleterious effects of immobilization of the skeletal striated muscle, contributing to the development of muscle atrophy due to disuse. Methods: Forty Wistar rats were divided into four groups: 1) Control Group (CG), 2) Periodontal Disease (GPD) 3) Immobilized (IG) and 4) Immobilized with Periodontal Disease (IPDG). Periodontal disease was induced for 30 days, with ligature method, and the immobilization of the right pelvic limb was performed with cast bandage for 15 days. Prior to euthanasia, the nociceptive threshold and muscular grasping force were evaluated. Afterwards, the soleus muscle was dissected and processed for sarcomere counting and morphological and morphometric analysis. For analysis of the data, the one-way ANOVA followed by the Tukey post test, with p < 0.05. Results: The IG and IPDG groups presented lower muscle weight, lower muscular grip strength and less number of sarcomeres compared to CG. The PDG showed reduction of muscle strength and nociceptive threshold after 15 days of periodontal disease and increase of connective tissue compared to CG. The IPDG presented lower muscle length and nociceptive threshold compared to the other groups. The IG presented a reduction in the cross-sectional area and a smaller diameter, an increase in the number of nuclei and a nucleus/fiber ratio, a decrease in the number of capillaries and a capillary/fiber ratio, with an increase in connective tissue. In the IPDG group, there were significant results for increased nucleus/fiber ratio, decreased capillaries and increased connective tissue when compared to the IG group. The IPDG group presented greater muscle tissue degeneration and increased inflammatory cells when compared to the other groups. Conclusion: Periodontal disease potentiated the deleterious effects of immobilization of the skeletal striated muscle, through intense destruction of muscle tissue, with significant increase of connective tissue, nucleus/fiber ratio and inflammatory infiltrate, significant reduction of vascularization and reduction of muscle length, with consequent reduction of muscle strength and nociceptive threshold. Objetivo: Avaliar se a doença periodontal, por meio da inflamação sistêmica, potencializa os efeitos deletérios da imobilização do músculo estriado esquelético, colaborando para o desenvolvimento da atrofia muscular por desuso. Metodologia: Foram utilizados 40 ratos Wistar, divididos em quatro grupos: 1) Grupo Controle (GC); 2) Doença Periodontal (GDP); 3) Imobilizado (GI); 4) Doença Periodontal Imobilizado (GDPI). A doença periodontal foi induzida pelo método de ligadura, durante 30 dias e a imobilização do membro pélvico direito foi realizada com atadura gessada, por 15 dias. Antes da eutanásia, foram avaliados o limiar nociceptivo e força muscular de preensão. Após, o músculo sóleo foi dissecado e processado para contagem de sarcômeros e análise morfológica e morfométrica. Para análise dos dados, foi utilizado o teste ANOVA de uma via seguida do post test Tukey, com p

Objetivo: Avaliar a influência da postura em prona sobre o estresse no recém-nascido prematuro por meio da dosagem do cortisol salivar e da avaliação das respostas fisiológicas e comportamentais, antes e após o posicionamento. Métodos: Foi realizada a coleta de saliva em cada recém-nascido em dois momentos: o primeiro (correspondente ao basal), sem manipulação prévia por 40 minutos, em decúbito lateral ou supino; e o segundo, 30 minutos após o posicionamento em prona. A frequência cardíaca e respiratória, saturação periférica de oxigênio e escala de sono de Brazelton foram registradas antes, durante e ao final do posicionamento em prona. Resultados: Participaram do estudo 16 recém-nascidos prematuros (56,3% masculino) com idade gestacional de 26 a 36 semanas, com 1 a 33 dias de vida, e peso variando de 935 a 3.050g ao nascimento e de 870 a 2.890g no dia da intervenção. Durante o posicionamento, seis recém-nascidos estavam em ar ambiente e os demais recebiam oxigênio suplementar. A mediana dos níveis de cortisol salivar foi menor durante o posicionamento em prona comparativamente ao basal (0,13 e 0,20; p=0,003), assim como a do escore de sono de Brazelton (p=0,02). A média da frequência respiratória foi menor após a intervenção (54,88±7,15 e 60±7,59; p=0,0004). As demais variáveis analisadas não apresentaram variação significativa. Conclusão: O posicionamento em prona diminuiu significativamente os níveis de cortisol salivar, da frequência respiratória e do escore de sono de Brazelton, sugerindo a correlação entre essa postura e a diminuição do estresse nesses recém-nascidos. Objective: This study sought to assess the influence of prone positioning on the stress of newborn premature infants through the measurement of the salivary cortisol concentration and the evaluation of physiological and behavioral responses before and after changes in body positioning. Methods: Saliva samples were collected from newborn infants at two different times: the first (corresponding to the baseline) after a period of 40 minutes during which the infants were not subjected to any manipulation and were placed in the lateral or supine position, and the second 30 minutes after placement in the prone position. Variables including heart rate, respiratory rate, peripheral oxygen saturation, and the Brazelton sleep score were recorded before, during, and at the end of the period in the prone position. Results: The sample comprised 16 newborn premature infants (56.3% male) with a gestational age between 26 and 36 weeks, postnatal age between 1 and 33 days, birth weight of 935 to 3,050g, and weight at the time of intervention of 870 to 2,890g. During the intervention, six participants breathed room air, while the remainder received oxygen therapy. The median salivary cortisol concentration was lower in the prone position compared to baseline (0.13 versus 0.20; p=0.003), as was the median Brazelton sleep score (p=0.02). The average respiratory rate was lower after the intervention (54.88±7.15 versus 60±7.59; p=0.0004). The remainder of the investigated variables did not exhibit significant variation. Conclusion: Prone positioning significantly reduced the salivary cortisol level, respiratory rate, and Brazelton sleep score, suggesting a correlation between prone positioning and reduction of stress in preterm infants.