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Your search keyword '"Lelke, Michaela"' showing total 13 results
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13 results on '"Lelke, Michaela"'

2. Development and evaluation of antibody-capture immunoassays for detection of Lassa virus nucleoprotein-specific immunoglobulin M and G

Author

Gabriel, Martin, primary, Adomeh, Donatus I., additional, Ehimuan, Jacqueline, additional, Oyakhilome, Jennifer, additional, Omomoh, Emmanuel O., additional, Ighodalo, Yemisi, additional, Olokor, Thomas, additional, Bonney, Kofi, additional, Pahlmann, Meike, additional, Emmerich, Petra, additional, Lelke, Michaela, additional, Brunotte, Linda, additional, Ölschläger, Stephan, additional, Thomé-Bolduan, Corinna, additional, Becker-Ziaja, Beate, additional, Busch, Carola, additional, Odia, Ikponmwosa, additional, Ogbaini-Emovon, Ephraim, additional, Okokhere, Peter O., additional, Okogbenin, Sylvanus A., additional, Akpede, George O., additional, Schmitz, Herbert, additional, Asogun, Danny A., additional, and Günther, Stephan, additional

Published
2018
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3. Identification of unknown functions in Lassa virus L protein

Author

Lelke, Michaela, Dotzauer, Andreas, and Günther, Stephan

Subjects
capping, ddc:570, 570 Life sciences, biology, Lassa virus, endonuclease
Abstract

The central domain of the 200-kDa Lassa virus L protein is a putative RNA-dependent RNA polymerase. N- and C-terminal domains may harbor enzymatic functions important for viral mRNA synthesis, including capping enzymes or cap-snatching endoribonucleases. In this work it was shown that the N-terminal region in L protein is important for mRNA, but not for genome synthesis. Bioinformatics and cell biological experiments lend support to the hypothesis that this region could be part of a cap-snatching enzyme.

Published
2010

5. Hospital-Based Surveillance for Viral Hemorrhagic Fevers and Hepatitides in Ghana

Author

Bonney, Joseph Humphrey Kofi, primary, Osei-Kwasi, Mubarak, additional, Adiku, Theophilus Korku, additional, Barnor, Jacob Samson, additional, Amesiya, Robert, additional, Kubio, Chrysantus, additional, Ahadzie, Lawson, additional, Ölschläger, Stephan, additional, Lelke, Michaela, additional, Becker-Ziaja, Beate, additional, Pahlmann, Meike, additional, and Günther, Stephan, additional

Published
2013
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6. Structure of the Lassa Virus Nucleoprotein Revealed by X-ray Crystallography, Small-angle X-ray Scattering, and Electron Microscopy

Author

Brunotte, Linda, primary, Kerber, Romy, additional, Shang, Weifeng, additional, Hauer, Florian, additional, Hass, Meike, additional, Gabriel, Martin, additional, Lelke, Michaela, additional, Busch, Carola, additional, Stark, Holger, additional, Svergun, Dmitri I., additional, Betzel, Christian, additional, Perbandt, Markus, additional, and Günther, Stephan, additional

Published
2011
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7. Current Molecular Epidemiology of Lassa Virus in Nigeria

Author

Ehichioya, Deborah U., primary, Hass, Meike, additional, Becker-Ziaja, Beate, additional, Ehimuan, Jacqueline, additional, Asogun, Danny A., additional, Fichet-Calvet, Elisabeth, additional, Kleinsteuber, Katja, additional, Lelke, Michaela, additional, ter Meulen, Jan, additional, Akpede, George O., additional, Omilabu, Sunday A., additional, Günther, Stephan, additional, and Ölschläger, Stephan, additional

Published
2011
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9. The N-Terminal Domain of the Arenavirus L Protein Is an RNA Endonuclease Essential in mRNA Transcription

Author

Morin, Benjamin, primary, Coutard, Bruno, additional, Lelke, Michaela, additional, Ferron, François, additional, Kerber, Romy, additional, Jamal, Saïd, additional, Frangeul, Antoine, additional, Baronti, Cécile, additional, Charrel, Rémi, additional, de Lamballerie, Xavier, additional, Vonrhein, Clemens, additional, Lescar, Julien, additional, Bricogne, Gérard, additional, Günther, Stephan, additional, and Canard, Bruno, additional

Published
2010
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13. Improved Detection of Lassa Virus by Reverse Transcription-PCR Targeting the 5' Region of S RNA

Author

O¨lschla¨ger, Stephan, Lelke, Michaela, Emmerich, Petra, Panning, Marcus, Drosten, Christian, Hass, Meike, Asogun, Danny, Ehichioya, Deborah, Omilabu, Sunday, and Gu¨nther, Stephan

Abstract

ABSTRACTThe method of choice for the detection of Lassa virus is reverse transcription (RT)-PCR. However, the high degree of genetic variability of the virus poses a problem with the design of RT-PCR assays that will reliably detect all strains. Recently, we encountered difficulties in detecting some strains from Liberia and Nigeria in a commonly used glycoprotein precursor (GPC) gene-specific RT-PCR assay (A. H. Demby, J. Chamberlain, D. W. Brown, and C. S. Clegg, J. Clin. Microbiol. 32:2898-2903, 1994), which prompted us to revise the protocol. The design of the new assay, the GPC RT-PCR/2007 assay, took into account 62 S RNA sequences from all countries where Lassa fever is endemic, including 40 sequences generated from the strains in our collection. The analytical sensitivity of the new assay was determined with 11 strains from Sierra Leone, Liberia, Ivory Coast, and Nigeria by probit analysis; the viral loads detectable with a probability of 95% ranged from 342 to 2,560 S RNA copies/ml serum, which corresponds to 4 to 30 S RNA copies/assay. The GPC RT-PCR/2007 assay was validated with 77 serum samples and 1 cerebrospinal fluid sample from patients with laboratory-confirmed Lassa fever. The samples mainly originated from Liberia and Nigeria and included strains difficult to detect in the assay of 1994. The GPC RT-PCR/2007 assay detected virus in all clinical specimens (100% sensitivity). In conclusion, a new RT-PCR assay, based in part on the protocol developed by Demby et al. in 1994, for the detection of Lassa virus is described. Compared to the assay developed in 1994, the GPC RT-PCR/2007 assay offers improved sensitivity for the detection of Liberian and Nigerian Lassa virus strains.

Published
2010
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