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4 results on '"Lena Liboschik"'

1. In vitro neutrophil migration is associated with inhaled corticosteroid treatment and serum cytokines in pediatric asthma

Author

Solveig Lemmel, Markus Weckmann, Anna Wohlers, Adan Chari Jirmo, Ruth Grychtol, Isabell Ricklefs, Gyde Nissen, Anna Bachmann, Shantanu Singh, Juan Caicedo, Thomas Bahmer, Gesine Hansen, Erika Von Mutius, Klaus F. Rabe, Oliver Fuchs, Anna-Maria Dittrich, Bianca Schaub, Christine Happle, Anne E. Carpenter, Matthias Volkmar Kopp, Tim Becker, the ALLIANCE Study Group as part of the German Centre for Lung Research (DZL), Mustafa Abdo, Miguel Alcazar, Mira Berbig, Heike Biller, Xenia Bovermann, Folke Brinkmann, Mifflin-Rae Calveron, David S. DeLuca, Gesa Diekmann, Christian Dopfer, Markus Ege, Svenja Foth, Svenja Gaedcke, Karoline I. Gaede, Anika Habener, Christian Herzmann, Alexander Hose, Sabina Illi, Anne-Marie Kirsten, Naschla Kohistani-Greif, Inke R. König, Silke Van Koningsbruggen-Rietschel, Matthias V. Kopp, Johanna Kurz, Katja Landgraf-Rauf, Kristina Laubhahn, Lena Liboschik, Claudia Liebl, Berrit Liselotte Husstedt, Bin Liu, Nicole Maison, Aydin Malik, Carola Marzi, Meike Meyer, Catharina Nitsche, Frauke Pedersen, Mareike Price, Harald Renz, Ernst Rietschel, Barbara Roesler, Christina Schauberger, Tom Schildberg, Carsten Schmidt-Weber, Nicolaus Schwerk, Chrysanthi Skevaki, Alena Steinmetz, Laila Sultansei, Marlen Szewczyk, Dominik Thiele, Vera Veith, Gesche Voigt, Benjamin Waschki, Henrik Watz, Stefanie Weber, Nils Welchering, Esther Zeitlmann, and Ulrich Zissler

Subjects
neutrophil granulocytes, migration, LTB4, fMLP, high-content image analysis, single-cell analysis, Therapeutics. Pharmacology, RM1-950
Abstract

Background: Different asthma phenotypes are driven by molecular endotypes. A Th1-high phenotype is linked to severe, therapy-refractory asthma, subclinical infections and neutrophil inflammation. Previously, we found neutrophil granulocytes (NGs) from asthmatics exhibit decreased chemotaxis towards leukotriene B4 (LTB4), a chemoattractant involved in inflammation response. We hypothesized that this pattern is driven by asthma in general and aggravated in a Th1-high phenotype.Methods: NGs from asthmatic nd healthy children were stimulated with 10 nM LTB4/100 nM N-formylmethionine-leucyl-phenylalanine and neutrophil migration was documented following our prior SiMA (simplified migration assay) workflow, capturing morphologic and dynamic parameters from single-cell tracking in the images. Demographic, clinical and serum cytokine data were determined in the ALLIANCE cohort.Results: A reduced chemotactic response towards LTB4 was confirmed in asthmatic donors regardless of inhaled corticosteroid (ICS) treatment. By contrast, only NGs from ICS-treated asthmatic children migrate similarly to controls with the exception of Th1-high donors, whose NGs presented a reduced and less directed migration towards the chemokines. ICS-treated and Th1-high asthmatic donors present an altered surface receptor profile, which partly correlates with migration.Conclusions: Neutrophil migration in vitro may be affected by ICS-therapy or a Th1-high phenotype. This may be explained by alteration of receptor expression and could be used as a tool to monitor asthma treatment.

Published
2022
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2. Multicentre feasibility of multiple-breath washout in preschool children with cystic fibrosis and other lung diseases

Author

Mirjam Stahl, Cornelia Joachim, Ines Kirsch, Tatjana Uselmann, Yin Yu, Nadine Alfeis, Christiane Berger, Rebecca Minso, Isa Rudolf, Cornelia Stolpe, Xenia Bovermann, Lena Liboschik, Alena Steinmetz, Dunja Tennhardt, Friederike Dörfler, Jobst Röhmel, Klaudia Unorji-Frank, Claudia Rückes-Nilges, Bianca von Stoutz, Lutz Naehrlich, Matthias V. Kopp, Anna-Maria Dittrich, Olaf Sommerburg, and Marcus A. Mall

Subjects
Medicine
Abstract

Background Multiple-breath washout (MBW)-derived lung clearance index (LCI) detects early cystic fibrosis (CF) lung disease. LCI was used as an end-point in single- and multicentre settings at highly experienced MBW centres in preschool children. However, multicentre feasibility of MBW in children aged 2–6 years, including centres naïve to this technique, has not been determined systematically. Methods Following central training, 91 standardised nitrogen MBW investigations were performed in 74 awake preschool children (15 controls, 46 with CF, and 13 with other lung diseases), mean age 4.6±0.9 years at investigation, using a commercially available device across five centres in Germany (three experienced, two naïve to the performance in awake preschool children) with central data analysis. Each MBW investigation consisted of several measurements. Results Overall success rate of MBW investigations was 82.4% ranging from 70.6% to 94.1% across study sites. The number of measurements per investigation was significantly different between sites ranging from 3.7 to 6.2 (p

Published
2020
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3. T2-high asthma phenotypes across lifespan

Author

Nicole, Maison, Jimmy, Omony, Sabina, Illi, Dominik, Thiele, Chrysanthi, Skevaki, Anna-Maria, Dittrich, Thomas, Bahmer, Klaus Friedrich, Rabe, Markus, Weckmann, Christine, Happle, Bianca, Schaub, Meike, Meyer, Svenja, Foth, Ernst, Rietschel, Harald, Renz, Gesine, Hansen, Matthias Volkmar, Kopp, Erika, von Mutius, Ruth, Grychtol, Oliver, Fuchs, Barbara, Roesler, Nils, Welchering, Naschla, Kohistani-Greif, Johanna, Kurz, Katja, Landgraf-Rauf, Kristina, Laubhahn, Claudia, Liebl, Markus, Ege, Alexander, Hose, Esther, Zeitlmann, Mira, Berbig, Carola, Marzi, Christina, Schauberger, Ulrich, Zissler, Carsten, Schmidt-Weber, Isabell, Ricklefs, Gesa, Diekmann, Lena, Liboschik, Gesche, Voigt, Laila, Sultansei, Gyde, Nissen, Inke R, König, Anne-Marie, Kirsten, Frauke, Pedersen, Henrik, Watz, Benjamin, Waschki, Christian, Herzmann, Mustafa, Abdo, Heike, Biller, Karoline I, Gaede, Xenia, Bovermann, Alena, Steinmetz, Berrit Liselotte, Husstedt, Catharina, Nitsche, Vera, Veith, Marlen, Szewczyk, Folke, Brinkmann, Aydin, Malik, Nicolaus, Schwerk, Christian, Dopfer, Mareike, Price, Adan Chari, Jirmo, Anika, Habener, David S, DeLuca, Svenja, Gaedcke, Bin, Liu, Mifflin-Rae, Calveron, Stefanie, Weber, Tom, Schildberg, Silke, van Koningsbruggen-Rietschel, and Miguel, Alcazar

Subjects
Pulmonary and Respiratory Medicine, Lipopolysaccharides, Interleukin-13, Longevity, Allergens, Immunoglobulin E, Asthma, Eosinophils, Phenotype, CD28 Antigens, Eosinophilia, Humans, Interleukin-5, Biomarkers
Abstract

RationaleIn adults, personalised asthma treatment targets patients with type 2 (T2)-high and eosinophilic asthma phenotypes. It is unclear whether such classification is achievable in children.ObjectivesTo define T2-high asthma with easily accessible biomarkers and compare resulting phenotypes across all ages.MethodsIn the multicentre clinical All Age Asthma Cohort (ALLIANCE), 1125 participants (n=776 asthmatics, n=349 controls) were recruited and followed for 2 years (1 year in adults). Extensive clinical characterisation (questionnaires, blood differential count, allergy testing, lung function and sputum induction (in adults)) was performed at baseline and follow-ups. Interleukin (IL)-4, IL-5 and IL-13 were measured after stimulation of whole blood with lipopolysaccharide (LPS) or anti-CD3/CD28.Measurements and main resultsBased on blood eosinophil counts and allergen-specific serum IgE antibodies, patients were categorised into four mutually exclusive phenotypes: “atopy-only”, “eosinophils-only”, “T2-high” (eosinophilia + atopy) and “T2-low” (neither eosinophilia nor atopy). The T2-high phenotype was found across all ages, even in very young children in whom it persisted to a large degree even after 2 years of follow-up. T2-high asthma in adults was associated with childhood onset, suggesting early origins of this asthma phenotype. In both children and adults, the T2-high phenotype was characterised by excessive production of specific IgE to allergens (pConclusionsUsing easily accessible biomarkers, patients with T2-high asthma can be identified across all ages delineating a distinct phenotype. These patients may benefit from therapy with biologicals even at a younger age.

Published
2021

4. IgA

Author

Anika, Habener, Ruth, Grychtol, Svenja, Gaedcke, David, DeLuca, Anna-Maria, Dittrich, Christine, Happle, Mustafa, Abdo, Henrik, Watz, Frauke, Pedersen, Inke Regina, König, Dominik, Thiele, Matthias Volkmar, Kopp, Erika, von Mutius, Thomas, Bahmer, Klaus Friedrich, Rabe, Almut, Meyer-Bahlburg, Gesine, Hansen, Oliver, Fuchs, Barbara, Roesler, Nils, Welchering, Naschla, Kohistani-Greif, Johanna, Kurz, Katja, Landgraf-Rauf, Kristina, Laubhahn, Nicole, Maison, Claudia, Liebl, Bianca, Schaub, Markus, Ege, Sabina, Illi, Alexander, Hose, Esther, Zeitlmann, Mira, Berbig, Carola, Marzi, Christina, Schauberger, Ulrich, Zissler, Carsten, Schmidt-Weber, Isabell, Ricklefs, Gesa, Diekmann, Lena, Liboschik, Gesche, Voigt, Laila, Sultansei, Markus, Weckmann, Gyde, Nissen, Anne-Marie, Kirsten, Benjamin, Waschki, Christian, Herzmann, Heike, Biller, Karoline I, Gaede, Xenia, Bovermann, Alena, Steinmetz, Berrit Liselotte, Husstedt, Catharina, Nitsche, Vera, Veith, Marlen, Szewczyk, Folke, Brinkmann, Aydin, Malik, Nicolaus, Schwerk, Christian, Dopfer, Mareike, Price, Adan Chari, Jirmo, Bin, Liu, Mifflin-Rae, Calveron, Stefanie, Weber, Svenja, Foth, Chrysanthi, Skevaki, Harald, Renz, Meike, Meyer, Tom, Schildberg, Ernst, Rietschel, Silke, van Koningsbruggen-Rietschel, and Miguel, Alcazar

Subjects
Adult, Spirometry, Oscillometry, Respiratory System, Humans, Asthma, Immunoglobulin A
Abstract

Comprehensive studies investigated the role of T-cells in asthma which led to personalised treatment options targeting severe eosinophilic asthma. However, little is known about the contribution of B-cells to this chronic inflammatory disease. In this study we investigated the contribution of various B-cell populations to specific clinical features in asthma.In the All Age Asthma Cohort (ALLIANCE), a subgroup of 154 adult asthma patients and 28 healthy controls were included for B-cell characterisation by flow cytometry. Questionnaires, lung function measurements, blood differential counts and allergy testing of participants were analysed together with comprehensive data on B-cells using association studies and multivariate linear models.Patients with severe asthma showed decreased immature B-cell populations while memory B-cells were significantly increased compared with both mild-moderate asthma patients and healthy controls. Furthermore, increased frequencies of IgAWith this study we demonstrate for the first time a significant association of increased IgA

Published
2021

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