1. Fetal Cells in Maternal Blood: A Comparison of Methods for Cell Isolation and Identification
- Author
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Lene Lykke-Hansen, Steen Kølvraa, Andreas Plesch, Steen Smidt-Jensen, Thomas Lörch, Henning Djursing, Jens Bang, Britta Christensen, Dhanesh Gohel, John Philip, Jens Michael Hertz, and Ilona Hromadnikova
- Subjects
Male ,Embryology ,Erythroblasts ,Lipopolysaccharide Receptors ,Cell Separation ,Maternal blood ,Bioinformatics ,Blood cell ,Fetal cell ,Antigens, CD ,Pregnancy ,Receptors, Transferrin ,Centrifugation, Density Gradient ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Cell isolation ,In Situ Hybridization, Fluorescence ,Fetus ,business.industry ,Obstetrics and Gynecology ,General Medicine ,Fetal Blood ,Globins ,Antigens, Differentiation, B-Lymphocyte ,medicine.anatomical_structure ,Chorionic Villi Sampling ,Pediatrics, Perinatology and Child Health ,Immunology ,Leukocyte Common Antigens ,Female ,Identification (biology) ,Selection method ,business - Abstract
Objective: A variety of methods have been used to select and identify fetal cells from maternal blood. In this study, a commonly used 3-step selection method is compared with selection directly from whole blood. Identification of fetal origin by XY FISH of male cells was also evaluated. Methods: Maternal blood was drawn either before invasive chorion villus sampling (pre-CVS) or after (post-CVS) from women carrying a male fetus. Fetal cells were isolated either by density gradient centrifugation succeeded by CD45/CD14 depletion and CD71-positive selection from CD45/CD14-negative cells, or by CD71-positive selection directly from whole blood. The true origin of fetal cells recovered by the two methods was established by two rounds of XY chromosome FISH in reverse colors, in some instances combined with anti-zeta (ζ) or anti-ζ/anti-gamma (γ) antibody staining. Results: In blood samples taken post-CVS and enriched by CD71 selection directly from whole blood, fetal cells were identified with a frequency that was almost four orders of magnitude higher than in post-CVS samples enriched by the 3-step method. In blood samples taken pre-CVS and enriched by the 3-step procedure, no fetal cells were identified by reverse color FISH in 371 ml of blood. In similar samples enriched by CD71 selection on whole blood, two fetal cells were identified in 27 ml of blood. Rehybridization with X and Y chromosome probes with reverse colors was necessary to exclude false Y chromosome signals. Not all fetal cells identified by the presence of a true Y chromosome signal stained with anti-ζ antibody. Conclusions: Selection of fetal NRBCs from maternal blood by CD71-positive selection directly from whole blood is superior to density gradient centrifugation succeeded by CD45/CD14 depletion and CD71 selection of CD45/CD14-negative cells. Combining two markers for fetal origin is recommended for unambiguously identifying a cell as fetal.
- Published
- 2005