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The interaction between the sensitive interfaces of photoelectrochemical (PEC) semiconductor nanomaterials and microscopic matter creates endless potential for the efficient detection of endocrine disruptor. This work presents the development of a high-efficiency PEC aptasensor for bisphenol A (BPA) monitoring based on Cu3BiS3 sensitized CuV2O6 nanocomposites with exceptional visible-light PEC activity. We implemented the integration of Cu3BiS3 nanosheet photosensitizer to sensitize the CuV2O6 nanowire structure that was synthesized utilizing a facile hydrothermal approach. The band gap alignment between Cu3BiS3 and CuV2O6 facilitated enduring PEC response yielding an efficient interfacial structure. The surface of the CuV2O6/Cu3BiS3 electrode was modified with BPA aptamer, enabling specific binding with BPA and precise quantification of its content. The developed aptamer sensors possess a wide detection range of 5.00 × 10−1 to 5.00 × 104 ng/mL, and a low detection limit of 1.60 × 10−1 ng/mL (at S/N = 3). After undergoing 20 testing cycles and enduring long-term storage, the sensor maintained its stability and showcased excellent repeatability and reproducibility. This study presents a promising methodology for the detection of BPA in environmental settings. [ABSTRACT FROM AUTHOR]

A photoelectrochemical (PEC) biosensor capable of detecting cytokeratin 19 fragment 21-1 (CYFRA21-1) was optimized by taking advantage of the powerful conjugate repeats of horseradish peroxidase and tyramine (HRP-tyramine)–triggered enzymatic biocatalytic precipitation (BCP) on high-performance BiVO4/Ag3VO4/SnS2 photoelectrodes. Compared with the ubiquitous BCP strategy, we identified a design supporting conjugate repeats generated by HRP and tyramine–triggered immeasurable insoluble precipitates in the presence of hydrogen peroxide and 4-chloro-1-phenol (4-CN), and the steric hindrance improved sensitivity. Moreover, by virtue of BiVO4, Ag3VO4, SnS2 excellent level matching structure and chemical stability, a heterojunction (BiVO4/Ag3VO4/SnS2) with high light absorption efficiency has been successfully prepared. The novel heterostructure system of BiVO4/Ag3VO4/SnS2 with high detection current and low background signal exhibited high-performance PEC determination. Generally, the hitherto untapped biosensor resource realized the sensitive detection of CYFRA21-1 with a wide linear range from 50 fg/mL to 200 ng/mL, and a detection limit of 15 fg/mL, which illustrated the potential for biotechnological applications. [ABSTRACT FROM AUTHOR]

Herein, a smart competitive-type photoelectrochemical (PEC) sensor based on an antigen-down (Ag-down) format for procalcitonin (PCT) detection is proposed. A fullerene sensitized flower-like Sn3O4 nano-structure is used as the photoactive platform, and FeS2 is labeled on the secondary antibody as a signal adjusting element. The sensor exhibits excellent sensitivity and great stability. [ABSTRACT FROM AUTHOR]

Herein, a smart competitive-type photoelectrochemical (PEC) sensor based on an antigen-down (Ag-down) format for procalcitonin (PCT) detection is proposed. A fullerene sensitized flower-like Sn3O4 nano-structure is used as the photoactive platform, and FeS2 is labeled on the secondary antibody as a signal adjusting element. The sensor exhibits excellent sensitivity and great stability. [ABSTRACT FROM AUTHOR]

Herein, a novel dual mode detection system of split-type photoelectrochemical (PEC) and visual immunoassay was developed to detect neuron specific enolase (NSE), which achieved simultaneous and reliable NSE detection due to the completely different signal readouts and transduction mechanism. Specifically, specific reactions of antigens and antibodies were performed in 96-microwell plates. Gold nanoparticle (Au NP)-loaded Fe 3 O 4 (Au@Fe 3 O 4 ) NPs were used as secondary antibody markers and signal regulators, which could produce a blue-colored solution in the presence of 3,3',5,5'-tetramethylbenzidine (TMB) and H 2 O 2 because of its peroxidase-like activity. Therefore, the visual detection of NSE was realized, making the results more intuitive. Meanwhile, the above biological process could also be used as part of the split-type PEC sensing platform. Oxidized TMB and Fe 3+ were consumptive agents of the electron donor, which both realized the double quenching of PEC signal generated by the SnO 2 /MgIn 2 S 4 /Zn 0.1 Cd 0.9 S composites. Owing to the waterfall band structure, SnO 2 /MgIn 2 S 4 /Zn 0.1 Cd 0.9 S composites partially absorb visible light and effectively inhibit the electron-hole recombination, thereby providing significantly enhanced and stable initial signal. On the basis of the multiple signal amplification strategy and the split-type mode, NSE could be sensitively detected with a low detection limit of 14.0 fg·mL -1 (S/N = 3) and a wide linear range from 50.0 fg·mL -1 to 50.0 ng·mL -1 .

Herein, a split-type immunoassay strategy instigated by cation exchange (CE) and changing the capacity of an electron donor in an electrolyte solution is optimized, namely, for differentiating the biological-specific binding assay and photoelectrochemical (PEC) analysis. MoSe 2 /CdSe, a Z-scheme heterojunction with efficient visible light absorption and a low recombination of carriers, is used as a photoelectrode substrate. Silver ions (Ag + ) as the initiator of CE are generated by the acidolysis of evenly loaded silver nanoparticles on mesoporous silica nanospheres (MSNs). The theoretical calculation and experimental results confirm that Ag + replaces Cd 2+ in CdSe and retains the crystal structure of MoSe 2 . However, this behavior destroys the perfectly matched heterojunction structure and introduces defects, which led to the reduction of the photocurrent response. In addition, ascorbate oxidase in combination with MSNs can be used as a consumptive agent of the electron donor, which further improves the sensitivity and reliability of the sensor. As a proof of principle, neuron-specific enolase was applied to elucidate the potential application of the PEC immunoassay in clinical diagnosis, and the obtained linear range of the sensor was from 0.0001 to 100 ng/mL with a detection limit of 28 fg/mL (S/N = 3).