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Rhodol is an ideal platform for fluorescent probes owing to its spirolactone framework and excellent photochemical properties. Herein, a novel rhodol-based colorimetric and fluorescent turn-on probe, DER-1 , for the detection of Pd 0 , was rationally developed with an allyl carbamate group as the response unit. Base on the Pd 0 -triggered cleavage reaction and rhodol spiroring-opening mechanism, the proposed probe exhibited a high selectivity and sensitivity towards Pd 0 . Upon addition of Pd(PPh 3 ) 4 , a significant fluorescence enhancement at 547 nm was observed with an obvious color change from colorless to pink, which can be easily identified by naked-eye. In addition, the fluorescence intensity at 547 nm was linearly proportional to the concentration of Pd 0 in the range of 0–1.5 μM, and the detection limit was calculated to be 1.14 nM. That is, probe DER-1 can be quite a sensitive fluorescent turn-on probe for the quantitative detection of Pd 0 in pretty low dose. [ABSTRACT FROM AUTHOR]

Silole-core phenylacetylene dendrimers were designed and synthesized, among them, the model compound ( n = 0) and the first generation of the dendrimer ( n = 1) were obtained by the reaction of 2,5-dibromosilole with corresponding terminal alkynes, the second generation of the dendrimers ( n = 2) was synthesized from 2,5-diiodosilole. These compounds indicated the absorptions of both phenylacetylene dendrons (250-350 nm) and silole core (400-500 nm). The first generation displayed efficient energy transfer from phenylacetylene dendrons to silole core, whose energy transfer efficiency was as high as 80%. These compounds were used as chemical sensors to probe explosive, for picric acid (PA), the Stern-Volmer constants of model compound and the first generation are 7120 and 5490 M−1, respectively. J. Heterocyclic Chem., (2012). [ABSTRACT FROM AUTHOR]

Fe3O4@ZrO2 microspheres with well-defined core-shell structure were prepared and applied for the highly selective enrichment of phosphopeptides from tryptic digest product of proteins. To successfully coat iron oxide microspheres with uniform zirconia shell, magnetic Fe3O4 microspheres were first synthesized via a solvothermal reaction, followed by being coated with a thin layer of carbon by polymerization and carbonization of glucose through hydrothermal reaction. Finally, with the use of the Fe3O4@C microspheres as templates, zirconium isopropoxide was prehydrolyzed and absorbed onto the microspheres and eventually converted into zirconia by calcinations. The as-prepared Fe3O4@ZrO2 core-shell microspheres were used as affinity probes to selectively concentrate phosphopeptides from tryptic digest of beta-casein, casein, and five protein mixtures to exemplify their selective enrichment ability of phosphopeptides from complex protein samples. In only 0.5 min, phosphopeptides sufficient for characterization by MALDI-MS could be enriched by the Fe3O4@ZrO2 microspheres. The results demonstrate that Fe3O4@ZrO2 microspheres have the excellent selective enrichment capacity for phosphopeptides from complex samples. The performance of the Fe3O4@ZrO2 microspheres was further compared with commercial IMAC beads for the enrichment of peptides originating from tryptic digestion of beta-casein and bovine serum albumin (BSA) with a molar ratio of 1:50, and the results proved a stronger selective ability of Fe3O4@ZrO2 microspheres over IMAC beads. Finally, the Fe3O4@ZrO2 microspheres were successfully utilized for enrichment of phosphopeptides from human blood serum without any other purification procedures.