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The systematic quality assurance (QA) and control of testing methods in incorporation monitoring consists of continual measures for internal QA and additional measures such as external laboratory controls. This includes among other aspects accuracy, precision and descriptions of the methods as well as the representation and timely availability of analytic results of measurements and internal dose assessment. At the officially recognised incorporation measurement office at Jülich, QA is performed for direct measurements (whole-body counter), indirect measurements with radiochemical testing methods of excretion samples and internal dose assessment. [ABSTRACT FROM PUBLISHER]

The accreditation of the Competent Incorporation Measuring Body at Jülich includes incorporation monitoring by means of direct measurements of the body activity as well as by means of indirect determination of the body activity by radiochemical analysis of excreta samples. In both testing areas, it proved to be very useful to have a flexible scope. In particular, the associated freedom in choosing testing procedures supports the continual improvement process of the laboratory. The modification of existing methods as well as the development and introduction of new procedures makes an immediate reaction to changed requirements feasible. At Jülich the use made out of the flexible scope included, e.g. the introduction of mathematical calibration in whole-body counting and the automation of sample preparation in radiochemical analysis. Advantages of the new procedures and modified methods include on the one hand the reduction of processing times, downtimes and hazard potentials on the other hand enhanced detection limits and improved cost-efficiency. In the result, it can be recommended to other qualified testing laboratories to go for a flexible scope. [ABSTRACT FROM PUBLISHER]

Understanding the athlete's movements and the restrictions incurred by protective equipment is crucial for improving the equipment and subsequently, the athlete's performance. The task of equipment improvement is especially challenging in sports including advanced manoeuvres such as ice hockey and requires a holistic approach guiding the researcher's attention toward the right variables. The purposes of this study were (a) to quantify the effects of protective equipment in ice hockey on player's performance and (b) to identify the restrictions incurred by it. Twenty male hockey players performed four different drills with and without protective equipment while their performance was quantified. A neural network accompanied by layer-wise relevance propagation was applied to the 3D kinematic data to identify variables and time points that were most relevant for the neural network to distinguish between the equipment and no equipment condition, and therefore presumable result from restrictions incurred by the protective equipment. The study indicated that wearing the protective equipment, significantly reduced performance. Further, using the 3D kinematics, an artificial neural network could accurately distinguish between the movements performed with and without the equipment. The variables contributing the most to distinguishing between the equipment conditions were related to the upper extremities and movements in the sagittal plane. The presented methodology consisting of artificial neural networks and layer-wise relevance propagation contributed to insights without prior knowledge of how and to which extent joint angles are affected in complex maneuvers in ice hockey in the presence of protective equipment. It was shown that changes to the equipment should support the flexion movements of the knee and hip and should allow players to keep their upper extremities closer to the torso., Competing Interests: This work was funded by CCM Hockey (Montreal, Canada) and Biomechanigg Sport and Health Research (BSHR; Calgary, Canada). CCM Hockey also funded subject recruitment and data collection. However, the results presented in this article do not in any way represent a bias toward CCM products over other brands. The principal investigator, Dr. Benno Nigg, is also the Chief Science Officer of the sponsoring company BSHR. BSHR covered material costs for this research and was simply interested in the outcome of the study, regardless of the findings. The company BSHR did not benefit from the results of the findings. BSHR had no influence on the outcome of this study. The results of the study were presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This does not alter our adherence to PLOS ONE policies on sharing data and materials., (Copyright: © 2024 Lennartz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Background: Prior studies investigated selected discrete sagittal-plane outcomes (e.g., peak knee flexion) in relation to running economy, hereby discarding the potential relevance of running technique parameters during noninvestigated phases of the gait cycle and in other movement planes., Purpose: Investigate which components of running technique distinguish groups of runners with better and poorer economy and higher and lower weekly running distance using an artificial neural network (ANN) approach with layer-wise relevance propagation., Methods: Forty-one participants (22 males and 19 females) ran at 2.78 m∙s -1 while three-dimensional kinematics and gas exchange data were collected. Two groups were created that differed in running economy or weekly training distance. The three-dimensional kinematic data were used as input to an ANN to predict group allocations. Layer-wise relevance propagation was used to determine the relevance of three-dimensional kinematics for group classification., Results: The ANN classified runners in the correct economy or distance group with accuracies of up to 62% and 71%, respectively. Knee, hip, and ankle flexion were most relevant to both classifications. Runners with poorer running economy showed higher knee flexion during swing, more hip flexion during early stance, and more ankle extension after toe-off. Runners with higher running distance showed less trunk rotation during swing., Conclusion: The ANN accuracy was moderate when predicting whether runners had better, or poorer running economy, or had a higher or lower weekly training distance based on their running technique. The kinematic components that contributed the most to the classification may nevertheless inform future research and training., (© 2024 The Authors. Scandinavian Journal of Medicine & Science In Sports published by John Wiley & Sons Ltd.)

A laboratory exercise is described in which students in a neuroscience, psychobiology, or similar laboratory course record the electromyogram (EMG) from themselves, using surface electrodes (placed on the skin). This exercise is intended to give students a firsthand demonstration that electrical activity is produced within them and to allow the students to use this activity to study biological and psychological concepts. The students study the nature of the EMG (changes with tension and the temporal relationship with limb movement) and the concepts of flexion and extension, reaction time, and patellar ("knee jerk") reflex. In postlaboratory evaluations, undergraduate introductory neuroscience students indicated that they appreciated the opportunity to record electrical activity from their own bodies. The students found the exercise enjoyable, believed that they had learned from it, and indicated that it should be a regular part of the course. If electrophysiology in animal preparations is already part of the course, this exercise requires minimal additional equipment, some of which is easily constructed and the reminder of which is available inexpensively.

Both systemic and central injections of glucose can enhance memory. For example, glucose reverses impairments on inhibitory avoidance resulting from intra-amygdala injections of morphine. The present experiment investigated the ability of glucose to reverse memory impairments resulting from intra-amygdala injections of propranolol, a beta-noradrenergic antagonist. Pretraining administration of 10 microg propranolol significantly reduced inhibitory avoidance retention latencies but had no effect on performance in a spontaneous alternation task. Coadministration of glucose into the amygdala at 3 doses (1.5, 3.0, and 6.0 microg) did not reverse the propranolol-induced inhibitory avoidance deficits. However, coadministration of 2.5 microg of glutamate with the propranolol did reverse these deficits. The ability of glucose to reverse impairments following intra-amygdala injections of morphine but not propranolol may reflect the neurotransmitter system or systems through which glucose exerts its effects.

Glucose infusions into the medial septal area attenuate memory impairments produced by concurrent intraseptal morphine injections. One possible explanation for these effects of glucose on memory is that the treatment modulates regional energy metabolism. As a test of this hypothesis, the present experiment determined whether intraseptal pyruvate injections could attenuate a spontaneous alternation impairment seen after intraseptal morphine injections. Intraseptal injections of morphine (4.0 nmol) 30 min prior to testing produced spontaneous alternation scores significantly lower than those in control groups. Morphine injections near, but outside, the septal region did not impair spontaneous alternation performance. The morphine-induced impairment was similarly reversed by coadministration of either glucose (18 nmol) or pyruvate (18 nmol) into the septum. These findings suggest that glucose may act through the tricarboxylic acid cycle by increasing the availability of ATP, augmenting the synthesis of certain neurotransmitters, or both.

N-Methyl-D-aspartate (NMDA) antagonists have been demonstrated to impair acquisition in a variety of tasks, including maze learning. It was previously reported from this laboratory that glucose can reverse the deficits on spontaneous alternation resulting from administration of the competitive NMDA antagonist NPC 12626 in mice. The present study tested the ability of glucose to reverse deficits induced by the noncompetitive NMDA antagonist MK-801. Although subcutaneous administration of 0.10 mg/kg of MK-801 resulted in a deficit on spontaneous alternation, glucose (100 and 250 mg/kg) did not reverse the impairment. This difference in the ability of glucose to reverse the impairment caused by the two NMDA antagonists may reflect their different modes of actions at the NMDA receptor complex.

Lemniscal and non-lemniscal parallel thalamocortical auditory pathways have been identified with the ventral medial geniculate body (MGB) vs. the dorsal and medial MGB, respectively. Lemniscal neurons have narrow frequency tuning and provide highly specific frequency information to the auditory cortex whereas non-lemniscal neurons generally have broader tuning and greater response lability, including plasticity of frequency receptive fields during learning. To determine if frequency selectivity is related to temporal fidelity of response, we measured both the breadth of tuning and neuronal excitability in a paired tone paradigm for single neurons throughout the MGB. Excitability to the second tone of a pair was directly correlated with frequency selectivity: the narrower the frequency tuning, the greater the excitability. Cells with broad tuning based on multiple-peak response areas also were less excitable than cells with single-peak RAs. Cells in the ventral MGB showed greater temporal fidelity of response (greater excitability) than cells in the dorsal and medial MGB. These findings show that high degrees of both frequency selectivity and temporal response fidelity are characteristic of the lemniscal, but not the non-lemniscal, thalamocortical auditory system.

Fear conditioning modifies the processing of frequency information; receptive fields (RF) in the auditory cortex and the medial geniculate body (MGB) are altered to favor processing the frequency of the conditioned stimulus (CS) over the pretraining best frequency (BF) and other frequencies. This experiment was designed to determine whether brief conditioning in the waking state produces RF plasticity that is expressed under general anesthesia. Guinea pigs bearing electrodes in the MGB received 20 trials of tone-shock pairing in a single training session. RFs were determined with animals under ketamine anesthesia before conditioning and 1-3 hr and 24 hr after conditioning. Frequency-specific RF plasticity was evident for both postconditioning periods: The BF shifted toward or to the CS frequency, responses to the BF decreased, and responses to the CS increased. Broadly tuned cells developed greater RF plasticity than narrowly tuned neurons. The results demonstrate that the specific neuronal results of brief learning experiences can be expressed in the anesthetized brain.