Search

Your search keyword '"Levesque, Roger C."' showing total 598 results
Start Over Author "Levesque, Roger C."
598 results on '"Levesque, Roger C."'

5. Genomic and Phenotypic Analysis of Salmonella enterica Bacteriophages Identifies Two Novel Phage Species

Author

Bhandare, Sudhakar, primary, Lawal, Opeyemi U., additional, Colavecchio, Anna, additional, Cadieux, Brigitte, additional, Zahirovich-Jovich, Yella, additional, Zhong, Zeyan, additional, Tompkins, Elizabeth, additional, Amitrano, Margot, additional, Kukavica-Ibrulj, Irena, additional, Boyle, Brian, additional, Wang, Siyun, additional, Levesque, Roger C., additional, Delaquis, Pascal, additional, Danyluk, Michelle, additional, and Goodridge, Lawrence, additional

Published
2024
Full Text
View/download PDF

6. Genomic and Phenotypic Analysis of Salmonella enterica Bac-Teriophages Identifies Two Novel Phage Species

Author

Bhandare, Sudhakar, primary, Lawal, Opeyemi U., additional, Colavecchio, Anna, additional, Cadieux, Brigitte, additional, Zahirovich-Jovich, Yella, additional, Zhong, Zeyan, additional, Tompkins, Elizabeth, additional, Amitrano, Margot, additional, Kukavica-Ibrulj, Irena, additional, Boyle, Brian, additional, Wang, Siyun, additional, Levesque, Roger C., additional, Delaquis, Pascal, additional, Danyluk, Michelle, additional, and Goodridge, Lawrence, additional

Published
2024
Full Text
View/download PDF

7. A Syst-OMICS Approach to Ensuring Food Safety and Reducing the Economic Burden of Salmonellosis.

Author

Emond-Rheault, Jean-Guillaume, Jeukens, Julie, Freschi, Luca, Kukavica-Ibrulj, Irena, Boyle, Brian, Dupont, Marie-Josée, Colavecchio, Anna, Barrere, Virginie, Cadieux, Brigitte, Arya, Gitanjali, Bekal, Sadjia, Berry, Chrystal, Burnett, Elton, Cavestri, Camille, Chapin, Travis K, Crouse, Alanna, Daigle, France, Danyluk, Michelle D, Delaquis, Pascal, Dewar, Ken, Doualla-Bell, Florence, Fliss, Ismail, Fong, Karen, Fournier, Eric, Franz, Eelco, Garduno, Rafael, Gill, Alexander, Gruenheid, Samantha, Harris, Linda, Huang, Carol B, Huang, Hongsheng, Johnson, Roger, Joly, Yann, Kerhoas, Maud, Kong, Nguyet, Lapointe, Gisèle, Larivière, Line, Loignon, Stéphanie, Malo, Danielle, Moineau, Sylvain, Mottawea, Walid, Mukhopadhyay, Kakali, Nadon, Céline, Nash, John, Ngueng Feze, Ida, Ogunremi, Dele, Perets, Ann, Pilar, Ana V, Reimer, Aleisha R, Robertson, James, Rohde, John, Sanderson, Kenneth E, Song, Lingqiao, Stephan, Roger, Tamber, Sandeep, Thomassin, Paul, Tremblay, Denise, Usongo, Valentine, Vincent, Caroline, Wang, Siyun, Weadge, Joel T, Wiedmann, Martin, Wijnands, Lucas, Wilson, Emily D, Wittum, Thomas, Yoshida, Catherine, Youfsi, Khadija, Zhu, Lei, Weimer, Bart C, Goodridge, Lawrence, and Levesque, Roger C

Subjects
Salmonella, antibiotic resistance, bacterial genomics, database, foodborne pathogen, next-generation sequencing, phylogeny, Genetics, Emerging Infectious Diseases, Biodefense, Infectious Diseases, Prevention, Vaccine Related, Foodborne Illness, Digestive Diseases, Human Genome, Infection, Good Health and Well Being, Environmental Science and Management, Soil Sciences, Microbiology
Abstract

The Salmonella Syst-OMICS consortium is sequencing 4,500 Salmonella genomes and building an analysis pipeline for the study of Salmonella genome evolution, antibiotic resistance and virulence genes. Metadata, including phenotypic as well as genomic data, for isolates of the collection are provided through the Salmonella Foodborne Syst-OMICS database (SalFoS), at https://salfos.ibis.ulaval.ca/. Here, we present our strategy and the analysis of the first 3,377 genomes. Our data will be used to draw potential links between strains found in fresh produce, humans, animals and the environment. The ultimate goals are to understand how Salmonella evolves over time, improve the accuracy of diagnostic methods, develop control methods in the field, and identify prognostic markers for evidence-based decisions in epidemiology and surveillance.

Published
2017

9. Design, Synthesis, and Evaluation of New 1H-Benzo[d]imidazole Based PqsR Inhibitors as Adjuvant Therapy for Pseudomonas aeruginosa Infections

Author

Soukarieh, Fadi, primary, Mashabi, Alaa, additional, Richardson, William, additional, Oton, Eduard Vico, additional, Romero, Manuel, additional, Dubern, Jean-Frédéric, additional, Robertson, Shaun N., additional, Lucanto, Simone, additional, Markham-Lee, Zoe, additional, Sou, Tomás, additional, Kukavica-Ibrulj, Irena, additional, Levesque, Roger C., additional, Bergstrom, Christel A. S., additional, Halliday, Nigel, additional, Kellam, Barrie, additional, Emsley, Jonas, additional, Heeb, Stephan, additional, Williams, Paul, additional, Stocks, Michael J., additional, and Cámara, Miguel, additional

Published
2024
Full Text
View/download PDF

10. Mitochondrial genome sequence of the protist Ancyromonas sigmoides Kent, 1881 (Ancyromonadida) from the Sugluk Inlet, Hudson Strait, Nunavik, Québec

Author

Gastineau, Romain, primary, Harðardóttir, Sara, additional, Guilmette, Caroline, additional, Lemieux, Claude, additional, Turmel, Monique, additional, Otis, Christian, additional, Boyle, Brian, additional, Levesque, Roger C., additional, Gauthier, Jeff, additional, Potvin, Marianne, additional, and Lovejoy, Connie, additional

Published
2023
Full Text
View/download PDF

11. The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration

Author

Brinkman, Fiona S.L., primary, Winsor, Geoffrey L., additional, Done, Rachel E., additional, Filloux, Alain, additional, Francis, Vanessa I., additional, Goldberg, Joanna B., additional, Greenberg, E. Peter, additional, Han, Kook, additional, Hancock, Robert E.W., additional, Haney, Cara H., additional, Häußler, Susanne, additional, Klockgether, Jens, additional, Lamont, Iain L., additional, Levesque, Roger C., additional, Lory, Stephen, additional, Nikel, Pablo I., additional, Porter, Steven L., additional, Scurlock, Matthew W., additional, Schweizer, Herbert P., additional, Tümmler, Burkhard, additional, Wang, Meng, additional, and Welch, Martin, additional

Published
2021
Full Text
View/download PDF

17. Rapid on-site detection of harmful algal blooms: real-time cyanobacteria identification using Oxford Nanopore sequencing

Author

Potvin, Marianne, primary, Gauthier, Jeff, additional, Langevin, Christophe, additional, Mohit, Vani, additional, da Costa, Naíla Barbosa, additional, Deschênes, Thomas, additional, Pomerleau, Maude, additional, Kukavica-Ibrulj, Irena, additional, Verreault, Daniel, additional, Comte, Jérôme, additional, and Levesque, Roger C., additional

Published
2023
Full Text
View/download PDF

30. Genetic diversity of Salmonella enterica isolated over 13 years from raw California almonds and from an almond orchard.

Author

Moyne, Anne-laure, Lawal, Opeyemi U., Gauthier, Jeff, Kukavica-Ibrulj, Irena, Potvin, Marianne, Goodridge, Lawrence, Levesque, Roger C., and Harris, Linda J.

Subjects
ALMOND, GENETIC variation, ALMOND growing, SALMONELLA enterica, SALMONELLA enteritidis, SALMONELLA, AGRICULTURE, GENOMICS, WHOLE genome sequencing
Abstract

A comparative genomic analysis was conducted for 171 Salmonella isolates recovered from raw inshell almonds and raw almond kernels between 2001 and 2013 and for 30 Salmonella Enteritidis phage type (PT) 30 isolates recovered between 2001 and 2006 from a 2001 salmonellosis outbreak-associated almond orchard. Whole genome sequencing was used to measure the genetic distance among isolates by single nucleotide polymorphism (SNP) analyses and to predict the presence of plasmid DNA and of antimicrobial resistance (AMR) and virulence genes. Isolates were classified by serovars with Parsnp, a fast core-genome multi aligner, before being analyzed with the CFSAN SNP Pipeline (U.S. Food and Drug Administration Center for Food Safety and Applied Nutrition). Genetically similar (≤18 SNPs) Salmonella isolates were identified among several serovars isolated years apart. Almond isolates of Salmonella Montevideo (2001 to 2013) and Salmonella Newport (2003 to 2010) differed by ≤9 SNPs. Salmonella Enteritidis PT 30 isolated between 2001 and 2013 from survey, orchard, outbreak, and clinical samples differed by ≤18 SNPs. One to seven plasmids were found in 106 (62%) of the Salmonella isolates. Of the 27 plasmid families that were identified, IncFII and IncFIB plasmids were the most predominant. AMR genes were identified in 16 (9%) of the survey isolates and were plasmid encoded in 11 of 16 cases; 12 isolates (7%) had putative resistance to at least one antibiotic in three or more drug classes. A total of 303 virulence genes were detected among the assembled genomes; a plasmid that harbored a combination of pef, rck, and spv virulence genes was identified in 23% of the isolates. These data provide evidence of long-term survival (years) of Salmonella in agricultural environments. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

31. Post-COVID symptoms are associated with endotypes reflecting poor inflammatory and hemostatic modulation.

Author

Yi An, Andy, Baghela, Arjun, Zhang, Peter G. Y., Blimkie, Travis M., Gauthier, Jeff, Kaufmann, Daniel Elias, Acton, Erica, Lee, Amy H. Y., Levesque, Roger C., and Hancock, Robert E. W.

Subjects
POST-acute COVID-19 syndrome, COVID-19, HOSPITAL admission & discharge, GENE expression
Abstract

Introduction: Persistent symptoms after COVID-19 infection ("long COVID") negatively affects almost half of COVID-19 survivors. Despite its prevalence, its pathophysiology is poorly understood, with multiple host systems likely affected. Here, we followed patients from hospital to discharge and used a systems-biology approach to identify mechanisms of long COVID. Methods: RNA-seq was performed on whole blood collected early in hospital and 4-12 weeks after discharge from 24 adult COVID-19 patients (10 reported post-COVID symptoms after discharge). Differential gene expression analysis, pathway enrichment, and machine learning methods were used to identify underlying mechanisms for post-COVID symptom development. Results: Compared to patients with post-COVID symptoms, patients without post-COVID symptoms had larger temporal gene expression changes associated with downregulation of inflammatory and coagulation genes over time. Patients could also be separated into three patient endotypes with differing mechanistic trajectories, which was validated in another published patient cohort. The "Resolved" endotype (lowest rate of post-COVID symptoms) had robust inflammatory and hemostatic responses in hospital that resolved after discharge. Conversely, the inflammatory/hemostatic responses of "Suppressive" and "Unresolved" endotypes (higher rates of patients with post-COVID symptoms) were persistently dampened and activated, respectively. These endotypes were accurately defined by specific blood gene expression signatures (6-7 genes) for potential clinical stratification. Discussion: This study allowed analysis of long COVID whole blood transcriptomics trajectories while accounting for the issue of patient heterogeneity. Two of the three identified and externally validated endotypes ("Unresolved" and "Suppressive") were associated with higher rates of post-COVID symptoms and either persistently activated or suppressed inflammation and coagulation processes. Gene biomarkers in blood could potentially be used clinically to stratify patients into different endotypes, paving the way for personalized long COVID treatment. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

35. The Spruce Budworm Genome: Reconstructing the Evolutionary History of Antifreeze Proteins

Author

Béliveau, Catherine, primary, Gagné, Patrick, additional, Picq, Sandrine, additional, Vernygora, Oksana, additional, Keeling, Christopher I, additional, Pinkney, Kristine, additional, Doucet, Daniel, additional, Wen, Fayuan, additional, Spencer Johnston, J, additional, Maaroufi, Halim, additional, Boyle, Brian, additional, Laroche, Jérôme, additional, Dewar, Ken, additional, Juretic, Nikoleta, additional, Blackburn, Gwylim, additional, Nisole, Audrey, additional, Brunet, Bryan, additional, Brandão, Marcelo, additional, Lumley, Lisa, additional, Duan, Jun, additional, Quan, Guoxing, additional, Lucarotti, Christopher J, additional, Roe, Amanda D, additional, Sperling, Felix A H, additional, Levesque, Roger C, additional, and Cusson, Michel, additional

Published
2022
Full Text
View/download PDF

39. Salmonella enterica subsp. enterica virulence potential can be linked to higher survival within a dynamic in vitro human gastrointestinal model

Author

Cavestri, Camille, primary, Savard, Patricia, additional, Fliss, Ismail, additional, Emond-Rhéault, Jean-Guillaume, additional, Hamel, Jérémie, additional, Kukavica-Ibrulj, Irena, additional, Boyle, Brian, additional, Daigle, France, additional, Malo, Danielle, additional, Bekal, Sadjia, additional, Harris, Linda J., additional, Levesque, Roger C., additional, Goodridge, Lawrence, additional, and LaPointe, Gisèle, additional

Published
2022
Full Text
View/download PDF

40. Design and Evaluation of New Quinazolin-4(3 H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa

Author

Soukarieh, Fadi, Mashabi, Alaa, Richardson, William, Oton, Eduard Vico, Romero, Manuel, Grossman, Scott, Sou, Tomas, Liu, Ruiling, Halliday, Nigel, Kukavica-Ibrulj, Irena, Levesque, Roger C., Kellam, Barrie, Emsley, Jonas, Heeb, Stephan, Williams, Paul, Stocks, Michael J., Roberston, Shaun N., and Bergstrom, Christel A. S.

Subjects
Infectious Diseases
Abstract

P. aeruginosa (PA) continues to pose a threat to global public health due to its high levels of antimicrobial resistance (AMR). The ongoing AMR crisis has led to an alarming shortage of effective treatments for resistant microbes, and hence there is a pressing demand for the development of novel antimicrobial interventions. The potential use of antivirulence therapeutics to tackle bacterial infections has attracted considerable attention over the past decades as they hamper the pathogenicity of target microbes with reduced selective pressure, minimizing the emergence of resistance. One such approach is to interfere with the PA pqs quorum sensing system which upon the interaction of PqsR, a Lys-R type transcriptional regulator, with its cognate signal molecules 4-hydroxy-2-heptylquinoline (HHQ) and 2-heptyl-3-hydroxy-4-quinolone (PQS), governs multiple virulence traits and host-microbe interactions. In this study, we report the hit identification and optimization of PqsR antagonists using virtual screening coupled with whole cell assay validation. The optimized hit compound 61 ((R)-2-(4-(3-(6-chloro-4-oxoquinazolin-3(4H)-yl)-2-hydroxypropoxy)phenyl)acetonitrile) was found to inhibit the expression of the PA PpqsA promoter controlled by PqsR with an IC50 of 1 μM. Using isothermal titration calorimetry, a Kd of 10 nM for the PqsR ligand binding domain (PqsRLBD) was determined for 61. Furthermore, the crystal structure of 61 with PqsRLBD was attained with a resolution of 2.65 Å. Compound 61 significantly reduced levels of pyocyanin, PQS, and HHQ in PAO1-L, PA14 lab strains and PAK6085 clinical isolate. Furthermore, this compound potentiated the effect of ciprofloxacin in early stages of biofilm treatment and in Galleria mellonella infected with PA. Altogether, this data shows 61 as a potent PqsR inhibitor with potential for hit to lead optimization toward the identification of a PA QS inhibitor which can be advanced into preclinical development.

Published
2021

50. Design and Evaluation of New Quinazolin-4(3H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa

Author

Soukarieh, Fadi, primary, Mashabi, Alaa, additional, Richardson, William, additional, Oton, Eduard Vico, additional, Romero, Manuel, additional, Roberston, Shaun N., additional, Grossman, Scott, additional, Sou, Tomas, additional, Liu, Ruiling, additional, Halliday, Nigel, additional, Kukavica-Ibrulj, Irena, additional, Levesque, Roger C., additional, Bergstrom, Christel A. S., additional, Kellam, Barrie, additional, Emsley, Jonas, additional, Heeb, Stephan, additional, Williams, Paul, additional, Stocks, Michael J., additional, and Cámara, Miguel, additional

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results