Search

Your search keyword '"Li, Chian-Pei"' showing total 19 results
Start Over Author "Li, Chian-Pei"
19 results on '"Li, Chian-Pei"'

2. P984: DIFFERENTIAL IMPACTS OF DISTINCT MUTATION SUBTYPES ON ALTERED S100A8 EXPRESSION AND PHENOTYPIC HETEROGENEITY IN CALR-MUTANT MPN

Author

Chen, Ying-Ju, primary, Wang, Ying-Hsuan, additional, Lai, Yi-Hua, additional, Wang, Ming-Chung, additional, Huang, Cih-En, additional, Chen, Yi-Yang, additional, Wu, Yu-Ying, additional, Yang, Yao-Ren, additional, Tsou, Hsing-Yi, additional, LI, Chian-Pei, additional, Hsu, Chia-Chen, additional, and Chen, Chih-Cheng, additional

Published
2023
Full Text
View/download PDF

6. Comparison of antiplatelet antibody profiles between hepatitis C virus-associated immune thrombocytopenia and primary immune thrombocytopenia

Author

Huang, Cih-En, primary, Chen, Wei-Ming, additional, Wu, Yu-Ying, additional, Shen, Chien-Heng, additional, Hsu, Chia-Chen, additional, Li, Chian-Pei, additional, Chen, Min-Chi, additional, Chang, Jung-Jung, additional, Chen, Yi-Yang, additional, Lu, Chang-Hsien, additional, Shi, Chung-Sheng, additional, and Chen, Chih-Cheng, additional

Published
2020
Full Text
View/download PDF

7. Comparison of antiplatelet antibody profiles between hepatitis C virus-associated immune thrombocytopenia and primary immune thrombocytopenia.

Author

Huang, Cih-En, Chen, Wei-Ming, Wu, Yu-Ying, Shen, Chien-Heng, Hsu, Chia-Chen, Li, Chian-Pei, Chen, Min-Chi, Chang, Jung-Jung, Chen, Yi-Yang, Lu, Chang-Hsien, Shi, Chung-Sheng, and Chen, Chih-Cheng

Subjects
IDIOPATHIC thrombocytopenic purpura, HLA histocompatibility antigens, LEUCOCYTES, BLOOD cell count, ENZYME-linked immunosorbent assay
Abstract

Hepatitis C virus-associated immune thrombocytopenia (HCV-ITP) has been assumed to be one of secondary ITP and associated with antiplatelet antibodies. This study was to clarify the antibody profile in HCV-ITP compared with primary ITP. We enrolled 55 HCV-ITP, 30 primary ITP, 11 Helicobacter pylori-ITP, 21 HCV control, and 16 healthy volunteers. We reviewed their blood cell counts, autoimmune markers, and spleen size. We used enzyme-linked immunosorbent assay kit to detect the specific antibody to glycoproteins IIb/IIIa, Ia/IIa, Ib/IX, IV, and human leukocyte antigen (HLA) class I. Compared with primary ITP patients, HCV-ITP patients had an older age, lower white blood cell (WBC) count and fewer presented with severe thrombocytopenia. The rate of positive antibody detection was 63.6% for the HCV-ITP group higher than the rate of 40% for the primary ITP. In the HCV control, antiplatelet antibodies were detected in 38.1% patients and no one had more than two types of antibodies. The antiplatelet antibodies correlated to severer thrombocytopenia. An HLA class I antibody was associated with lower WBCs and larger spleen. In conclusion, HCV-ITP patients had a high rate of positive antiplatelet antibody. The antibodies were associated with not only lower platelets but also leukopenia and splenomegaly. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

8. Molecular heterogeneity unravelled by single‐cell transcriptomics in patients with essential thrombocythaemia

Author

Hsu, Chia‐Chen, primary, Chen, Ying‐Ju, additional, Huang, Cih‐En, additional, Wu, Yu‐Ying, additional, Wang, Ming‐Chung, additional, Pei, Sung‐Nan, additional, Liao, Chun‐Kai, additional, Lu, Chang‐Hsien, additional, Chen, Ping‐Tsung, additional, Tsou, Hsing‐Yi, additional, Li, Chian‐Pei, additional, Chuang, Wei‐Hsuan, additional, Chuang, Ching‐Kai, additional, Yang, Cheng‐Yu, additional, Lai, Yi‐Hua, additional, Lin, Yi‐Hsuan, additional, and Chen, Chih‐Cheng, additional

Published
2019
Full Text
View/download PDF

10. Molecular heterogeneity unravelled by single‐cell transcriptomics in patients with essential thrombocythaemia.

Author

Hsu, Chia‐Chen, Chen, Ying‐Ju, Huang, Cih‐En, Wu, Yu‐Ying, Wang, Ming‐Chung, Pei, Sung‐Nan, Liao, Chun‐Kai, Lu, Chang‐Hsien, Chen, Ping‐Tsung, Tsou, Hsing‐Yi, Li, Chian‐Pei, Chuang, Wei‐Hsuan, Chuang, Ching‐Kai, Yang, Cheng‐Yu, Lai, Yi‐Hua, Lin, Yi‐Hsuan, and Chen, Chih‐Cheng

Subjects
SOMATIC mutation, NUCLEOTIDE sequence, HETEROGENEITY, PATHOLOGY, TRANSCRIPTOMES
Abstract

Summary: Significant phenotypic heterogeneity exists in patients with all subtypes of myeloproliferative neoplasms (MPN), including essential thrombocythaemia (ET). Single‐cell RNA sequencing (scRNA‐Seq) holds the promise of unravelling the biology of MPN at an unprecedented level of resolution. Herein we employed this approach to dissect the transcriptomes in the CD34+ cells from the peripheral blood of seven previously untreated ET patients and one healthy adult. The mutational profiles in these patients were as follows: JAK2 V617F in two, CALR in three (one type I and two type II) and triple‐negative (TN) in two. Our results reveal substantial heterogeneity within this enrolled cohort of patients. Activation of JAK/STAT signalling was recognized in discrepant progenitor lineages among different samples. Significantly disparate molecular profiling was identified in the comparison between ET patients and the control, between patients with different driver mutations (JAK2 V617F and CALR exon 9 indel), and even between patients harbouring the same driver. Intra‐individual clonal diversity was also found in the CD34+ progenitor population of a patient, possibly indicating the presence of multiple clones in this case. Estimation of subpopulation size based on cellular immunophenotyping suggested differentiation bias in all analysed samples. Furthermore, combining the transcriptomic information with data from targeted sequencing enabled us to unravel key somatic mutations that are molecularly relevant. To conclude, we demonstrated that scRNA‐Seq extended our knowledge of clonal diversity and inter‐individual heterogeneity in patients with ET. The obtained information could potentially leapfrog our efforts in the elucidation of the pathogenesis of the disease. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

12. Quantitative competitive allele-specific TaqMan duplex PCR (qCAST-Duplex PCR) assay: a refined method for highly sensitive and specific detection of JAK2V617F mutant allele burdens

Author

Hsu, Chia-Chen, primary, Huang, Cih-En, additional, Wu, Yu-Ying, additional, Chen, Yi-Yang, additional, Lung, Jrhau, additional, Leu, Yu-Wei, additional, Li, Chian-Pei, additional, Tsou, Hsing-Yi, additional, Chuang, Wei-Hsuan, additional, Lu, Chang-Hsien, additional, and Chen, Chih-Cheng, additional

Published
2018
Full Text
View/download PDF

18. Aberrant Let7a/HMGA2Signaling Axis with Unique Clinical Phenotype in JAK2-Mutated Myeloproliferative Neoplasms

Author

Hsu, Chia-Chen, Chen, Chih-Cheng, Gau, Jyh-Pyng, Huang, Cih-En, You, Jie-Yu, Lung, Jrhau, Chen, YI-Yang, Ho, Hsing-Ying, Li, Chian-Pei, Lu, Chang-Hsien, and Lee, Kuan-Der

Abstract

High mobility group AT-hook 2 (HMGA2) is an architectural transcriptional factor that is negatively regulated by Let-7microRNA. Dysregulated HMGA2has been shown to exhibit a myeloproliferative phenotype in engineered mice. To decipher the Let-7-HMGA2axis in myeloproliferative neoplasms (MPN), we studied a cohort of 151 MPN patients. Overexpressed HMGA2was detected in about one-fifth of the cases, and it was more commonly seen in ET (26.9%, vs. 12.7% in PV, p=0.044). Compared to their counterparts, HMGA2-overexpressing patients had higher platelet counts, increased thromboembolic risk, and inferior thrombosis-free survival. Fluorescence in situ hybridizationanalysis showed that chromosomal translocation was not a major cause of HMGA2overexpression in MPN patients, yet there was an inverse correlation between the expression levels of let-7aand HMGA2. Furthermore, up-regulation of HMGA2was significantly associated with MPN patients carrying JAK2V617F mutation. In vitrostudies showed that Ba/F3 cells carried JAK2V617F (Ba/F3-JAK2V617F) had decreased let-7aand up-regulated HMGA2. Silencing of HMGA2in Ba/F3-JAK2V617Fcells resulted in growth inhibition coupled with a significant increase in apoptosis. Our findings suggest that, in a subset of JAK2-mutated MPN patients, Let-7-HMGA2axis plays a prominent role in the pathogenesis of the disease that leads to unique clinical phenotypes.

Published
2016
Full Text
View/download PDF

19. Quantitative competitive allele-specific TaqMan duplex PCR (qCAST-Duplex PCR) assay: a refined method for highly sensitive and specific detection of JAK2 V617F mutant allele burdens.

Author

Hsu CC, Huang CE, Wu YY, Chen YY, Lung J, Leu YW, Li CP, Tsou HY, Chuang WH, Lu CH, and Chen CC

Subjects
Alleles, Amino Acid Substitution, Cell Line, Tumor, Female, Humans, Male, Hematologic Neoplasms genetics, Janus Kinase 2 genetics, Mutation, Missense, Myeloproliferative Disorders genetics, Real-Time Polymerase Chain Reaction methods
Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results