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194 results on '"Li, Dong-Ling"'

12. Identification of autophagy‐related genes in diabetic foot ulcer based on bioinformatic analysis.

Author

Li, Dong‐Ling, Ding, Xin‐Yi, Long, Juan, He, Qiao‐Ling, Zeng, Qing‐Xiang, Lu, Na, and Zou, Meng‐Chen

Subjects
HYDROLASES, AUTOPHAGY, RESEARCH funding, APOPTOSIS, GENE expression, BIOINFORMATICS, DIABETIC foot, EPIDERMAL growth factor receptors, BIOMARKERS, CELL receptors, PHOSPHOPROTEINS
Abstract

Diabetic foot ulcer (DFU) complications involve autophagy dysregulation. This study aimed to identify autophagy‐related bioindicators in DFU. Differentially expressed genes (DEGs) between DFU and healthy samples were analysed from the Gene Expression Omnibus (GEO) datasets, GSE7014 and GSE29221. The roles of autophagy‐related DEGs were investigated using protein–protein interaction (PPI) networks, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, Gene Ontology (GO) enrichment, and Gene Set Enrichment Analysis (GSEA). Immune cell infiltration's correlation with these DEGs was also assessed. From the Human Autophagy Database (HADB), 232 autophagy‐related genes (ARGs) were identified, with an intersection of 17 key DEGs between GSE7014 and GSE29221. These genes are involved in pathways like autophagy–animal, NOD‐like receptor signalling, and apoptosis. In the protein network, epidermal growth factor receptor (EGFR) and phosphatase and tensin homologue (PTEN) showed significant interactions with ARGs. Survival analysis indicated the prognostic importance of calpain 2 (CAPN2), integrin subunit beta 1 (ITGB1), and vesicle‐associated membrane protein 3 (VAMP3). Lower immune scores were observed in the type 2 diabetes mellitus (DM2) group than in controls. Autophagy and ARGs significantly influence DFU pathophysiology. [ABSTRACT FROM AUTHOR]

Published
2024
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42. Integration of Demethylation-Activated DNAzyme with a Single Quantum Dot Nanosensor for Sensitive Detection of O6-Methylguanine DNA Methyltransferase in Breast Tissues

Author

Han, Yun, Li, Dong-ling, Han, Qian, Ma, Fei, and Zhang, Chun-yang

Abstract

O6-Methylguanine-DNA-methyltransferase (MGMT) is a demethylation protein that dynamically regulates the O6-methylguanine modification (O6MeG), and dysregulated MGMT is implicated in various malignant tumors. Herein, we integrate demethylation-activated DNAzyme with a single quantum dot nanosensor to sensitively detect MGMT in breast tissues. The presence of MGMT induces the demethylation of the O6MeG-caged DNAzyme and the restoration of catalytic activity. The activated DNAzyme then specifically cleaves the ribonucleic acid site of hairpin DNA to expose toehold sequences. The liberated toehold sequence may act as a primer to trigger a cyclic exponential amplification reaction for the generation of enormous signal strands that bind with the Cy5/biotin-labeled probes to form sandwich hybrids. The assembly of sandwich hybrids onto 605QD obtains 605QD-dsDNA-Cy5 nanostructures, inducing efficient FRET between the 605QD donor and Cy5 acceptor. Notably, the introduction of a mismatched base in hairpin DNA can greatly minimize the background and improve the signal-to-noise ratio. This nanosensor achieves a dynamic range of 1.0 × 10–8to 0.1 ng/μL and a detection limit of 155.78 aM, and it can screen MGMT inhibitors and monitor cellular MGMT activity with single-cell sensitivity. Moreover, it can distinguish the MGMT level in tissues of breast cancer patients and healthy persons, holding great potential in clinical diagnostics and epigenetic research studies.

Published
2024
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45. Flow-induced-vibration MEMS energy harvesting unit based on AlN film in silicon

Author

尚正国 Shang Zheng-guo, 李东玲 Li Dong-ling, 赵兴强 Zhao Xing-qiang, and 温志渝 Wen Zhi-yu

Subjects
Microelectromechanical systems, Materials science, Optics, Silicon, chemistry, Vortex-induced vibration, business.industry, chemistry.chemical_element, Optoelectronics, business, Energy harvesting, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials
Published
2013

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