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Myocardial infarction (MI) is a common type of ischemic heart disease that affects millions of people worldwide. In recent times, nanotechnology has become a very promising field with immense applications. The current exploration was conducted to synthesize the chitosan‐sodium alginate‐polyethylene glycol‐Ally isothiocyanate nanocomposites (CSP‐AIso‐NCs) and evaluate their beneficial roles against the isoproterenol (ISO)‐induced MI in rats. The CSP‐AIso‐NCs were prepared and characterized by several characterization techniques. The MI was initiated in the rats by the administration of 85 mg/kg of ISO for 2 days and treated with 10 and 20 mg/kg of CSP‐AIso‐NCs for 1 month. The changes in heart weight and bodyweight were measured. The cardiac function markers were assessed with echocardiography. The lipid profiles, Na+, K+, and Ca2+ ions, cardiac biomarkers, antioxidant parameters, and inflammatory cytokines were assessed using corresponding assay kits. The histopathological study was done on the heart tissues. The UV spectral analysis revealed the maximum peak at 208 nm, which confirms the formation of CSP‐AIso‐NCs. The FT‐IR analysis revealed the occurrence of different functional groups, and the crystallinity of the CSP‐AIso‐NCs was proved by the XRD analysis. DLS analysis indicated the size of the CSP‐AIso‐NCs at 146.50 nm. The CSP‐AIso‐NCs treatment increased the bodyweight and decreased the HW/BW ratio in the MI rats. The status of lipids was reduced, and HDL was elevated in the CSP‐AIso‐NCs administered to MI rats. CSP‐AIso‐NCs decreased the LVEDs, LVEDd, and NT‐proBNP and increased the LVEF level. The oxidative stress markers were decreased, and the antioxidants were increased by the CSP‐AIso‐NCs treatment in the MI rats. The Na+ and Ca+ ions were reduced, and the K+ ions were increased by the CSP‐AIso‐NCs. The interleukin‐1β and tumor necrosis factor‐α were also depleted, and Nrf‐2 was improved in the CSP‐AIso‐NCs administered to MI rats. The histological study revealed the ameliorative effects of CSP‐AIso‐NCs. Overall, our outcomes revealed that the CSP‐AIso‐NCs are effective against the ISO‐induced MI rats. Hence, it could be a hopeful therapeutic nanomedicine for MI treatment. [ABSTRACT FROM AUTHOR]

Objective: Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. Because BV infects humans through bites, scratches, and other injuries inflicted by macaques, the simple and rapid diagnosis of BV in field laboratories is of great importance to protect veterinarians, laboratory researchers, and support personnels from the threat of infection. Methods: Two recombinase polymerase amplification (RPA) assays with a closed vertical flow (VF) visualization strip (RPA-VF-UL27 and RPA-VF-US6) were developed that target two conserved genes combined with a one-off, closed visualization strip device. We compared the sensitivities and specificities of the two assays after optimization of the reaction conditions. The performance of RPA-VF-US6 at room temperature was determined to evaluate its potential in point-of-care (POC) testing. Result: RPA-VF-US6 specifically detected the positive plasmid control (rather than nucleic acids of herpesviruses) with a detection limit of 28 copies, while RPA-VF-UL27 had cross-reactivity with HSV-1, but even 3.4 copies of plasmid standards were readout by this assay. Moreover, RPA-VF-US6 had excellent performance at room temperature (the detection limit was 2,800 plasmid copies), indicating the potential of RPA-VF-US6 in POC testing. Conclusion: We developed two RPA assays for BV visualization diagnosis. RPA-VF-US6 is a simple, rapid, and specific detection method for BV. The entire reaction can be performed at a constant temperature within 30 min, suggesting the potential of RPA-VF-US6 for POC testing in field laboratories without sophisticated instruments. [ABSTRACT FROM AUTHOR]