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Objective To investigate the expression level of RING finger protein 187 （RNF187） and its effect on the proliferation and invasion of prostate cancer （PCa） cells. Methods The differences in the expression of RNF187 between PCa and normal tissues was mined through TCGA and GEO databases. Normal prostate epithelial cell line RWPE-1， and human PCa cell lines LNCaP， C4-2， DU145 and PC-3 cell lines were cultured in vitro. The expression levels of RNF187 mRNA in RWPE-1， LNCaP， C4-2， DU145 and PC-3 were determined by quantitative fluorescent real-time PCR （RT-qPCR）. The expression level of RNF187 protein was determined by Western blot. DU145， PC-3 cells were transfected with RNF187-siRNA1 and RNF187-siRNA2. The relative expression level of RNF187 mRNA was determined by RT-qPCR. Cell viability was detected by CCK-8 assay. Cell proliferation was measured by 5-ethynyl-2´-deoxyuridine （EdU） labeling. Cell migration and invasion were measured by Transwell chamber. The expression levels of RNF187， epithelial-mesenchymal transition （EMT） marker proteins and matrix metalloproteinase-9 （MMP-9） were determined by Western blot. Results In TCGA and GEO datasets， the expression level of RNF187 in the PCa tissues was significantly up-regulated than that in the normal prostate tissues. Patients with higher Gleason scores and higher staging had higher expression levels of RNF187 （all P < 0.05）. The expression levels of RNF187 mRNA and protein in PC-3， LNCaP， C4-2 and DU145 cells were significantly up-regulated than those in RWPE-1 cells （all P < 0.01）. The relative expression levels of RNF187 mRNA in DU145 and PC-3 cells in the RNF187-si1 and RNF187-si2 groups were significantly down-regulated than those in the control group （all P < 0.001）. After RNF187 knock-down， the viability， proliferation， invasion and migration capabilities of DU145 and PC-3 cells were significantly decreased， the relative expression levels of RNF187， N-cadherin， Vimentin and MMP-9 proteins were significantly down-regulated， whereas the relative expression levels of E-cadherin were significantly up-regulated （all P < 0.05）. Conclusion RNF187 is over-expressed in PCa tissues. Knock-down of RNF187 can reduce the cell viability， proliferation， invasion and migration of DU145 and PC-3 cells.

Dry devolatilization is a promising method for saving energy, but understanding the devolatilization process has been challenging. A static devolatilizer was designed to study the devolatilization process of a Styrene-butadiene-styrene (SBS) block copolymer solution at different temperatures using visualization technology. The study found that temperature, Reynolds number, and heat flux are key factors influencing the devolatilization process. The optimal residence time was determined to be 196 s and the suitable feed coefficient is 0.17. Additionally, a new dimensionless parameter (the LZ number) was introduced. A value closer to 1 indicated a more effective devolatilization process. The obtained thermodynamic parameters and LZ number can guide the design of industrial devolatilization devices. [ABSTRACT FROM AUTHOR]

The high spatial search complexity of the direct positioning method in passive positioning systems leads to long positioning time and high computational resource consumption. In response to this issue, this article proposes a fast localization scheme based on composite convolutional neural networks (CCNN), which can effectively explore the correlation between the position of the radiation source and the characteristics of the received signal. CCNN is a 20‐layer composite network based on fully convolutional network layer, which is composed of convolutional layers, batch normalization (BN) layers, and ReLU activation function layers with unidirectional connections. Then, CCNNs are adjusted and trained for positioning single and multiple radiation sources, respectively. Simulation results show that the computational time of the proposed method can be reduced by nearly 98% compared with the direct positioning scheme. Meanwhile, about 71.2% of positioning error's reduction is achieved. [ABSTRACT FROM AUTHOR]

This paper primarily proposes a deterioration model of reinforced concrete (RC) beams under marine atmospheric environment. Considering factors including corrosion initiation time, corrosion rate, corrosion non-uniformity and its effects on mechanical performances of steel bars, a comprehensive model was developed for the assessment of mechanical behaviour of RC beams under marine atmospheric environment. Also, to avoid the shortages brought by the classical truss theory, a modified compression field theory (MCFT) was applied to evaluate the effects of stirrups corrosion on the shear capacity of an RC beam. Then, according to a virtual case, the life-cycle performance of an RC beam was predicted through probability density evolution method (PDEM). Results approved the feasibility and effectiveness of the proposed model. Finally, further study work was pointed out.