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18 results on '"Li, Jing-Lu"'

5. The polarization of microglia and infiltrated macrophages in the injured mice spinal cords: a dynamic analysis

Author

Li, Jing-Lu, primary, Fu, Gui-Qiang, additional, Wang, Yang-Yang, additional, Bian, Ming-Ming, additional, Xu, Yao-Mei, additional, Zhang, Lin, additional, Chen, Yu-Qing, additional, Zhang, Nan, additional, Ding, Shu-Qin, additional, Wang, Rui, additional, Fang, Rui, additional, Tang, Jie, additional, Hu, Jian-Guo, additional, and Lü, He-Zuo, additional

Published
2023
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12. Preparation and properties of quercetin-incorporated high density polyethylene/low density polyethylene antioxidant multilayer film

Author

Li-xin Lu and Li‐Jing Lu

Subjects
0301 basic medicine, 030109 nutrition & dietetics, Antioxidant, Materials science, Mechanical Engineering, medicine.medical_treatment, Active packaging, 04 agricultural and veterinary sciences, General Chemistry, 040401 food science, 03 medical and health sciences, Low-density polyethylene, chemistry.chemical_compound, 0404 agricultural biotechnology, Chemical engineering, chemistry, medicine, General Materials Science, High-density polyethylene, Quercetin
Published
2018

13. [Effect of silencing lycB gene on the carotenoid synthesis in Haematococcus pluvialis]

Author

Wen-Fang, Gong, Li-Jing, Lu, Xin, Liu, Xi-Wen, Chen, and De-Fu, Chen

Subjects
Lycopene, Chlorophyta, Gene Expression Regulation, Plant, Gene Order, RNA Interference, Gene Silencing, Intramolecular Lyases, Plants, Genetically Modified, Carotenoids
Abstract

Haematococcus pluvialis is a freshwater planktonic single-cell microalgae. It will accumulate high amount of carotenoids under unfavorable environmental conditions. As one of carotenoids, lycopene is an important intermediate in the carotenoid biosynthesis pathway. Lycopene β-cyclase (LycB) is the key enzyme that catalyzes the circularization of lycopene to form β-carotene. In this study, we constructed a p1301-BS-RNAi vector using lycB from Dunaliella salina as the interference sequence with kanamycin and atrazine resistance marker, and then transformed it into H. pluvialis by electroporation. Sixteen independent transgenic lines were obtained after resistance selection, genome PCR, and RT-PCR analyses. Seven well-grown lines were selected to determine the contents of carotenoids by HPLC analysis after inducing by high light. The lycopene content in these lines was increased by 99.4% while the β-carotene content was decreased by 48.4%, indicating that the interference by heterogenous lycB could inhibit the conversion of lycopene into β-carotene. The amount of increase in lycopene was only 5% of the amount of decrease in β-carotene, indicating that 95% of the decreased β-carotene was converted into other metabolites. Therefore, in order to largely increase the lycopene content in H. pluvialis, it is necessary to coordinatively regulate other metabolic pathways.

Published
2013

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