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In this work, we report the first bioluminescent probe BP-PS for detecting H2Sn with high specificity and sensitivity. Owing to the bioluminescence imaging without requiring an excitation light source, tissue autofluorescence is eliminated and BP-PS shows a high signal-to-noise ratio. Moreover, BP-PS was successfully utilized to visualize endogenous H2Sn in live cells and a murine model of bacterial infection. [ABSTRACT FROM AUTHOR]

A bioluminescent probe, BP-HNO, which exhibits a turn-on response to nitroxyl with high sensitivity and selectivity, is reported for the first time in this work. BP-HNO is free from the interference of biological autofluorescence to afford a high signal-to-noise ratio for bioimaging, and was successfully applied to imaging nitroxyl in live cells and mice. [ABSTRACT FROM AUTHOR]

Formaldehyde (FA) plays an important role in living systems as a reactive carbonyl species (RCS). An abnormal degree of FA is known to induce neurodegeneration, cognitive decrease and memory loss owing to the formation of strong cross-link DNA and protein and other molecules. The development of efficient methods for biological FA detection is of great biomedical importance. Although a few one-photon FA fluorescent probes have been reported for imaging in living cells, probes excited by two photons are more suitable for bio-imaging due to their low background fluorescence, less photobleaching, and deep penetration depth. In this study, a two-photon fluorescent probe FATP1 for FA detection and bio-imaging in living cells and tissues was reported. The detection is based on the 2-aza-Cope sigmatropic rearrangement followed by elimination to release the fluorophore, resulting in both one- and two-photon excited fluorescence increase. The probe FATP1 showed a high sensitivity to FA with a detection limit of 0.2 μM. Moreover, FATP1 enabled the two-photon bio-imaging of FA in live HEK-293 cells and tissues with tissue-imaging depths of 40–170 μm. Furthermore, FATP1 could be applied for the monitoring of endogenous FA in live MCF-7 cells, presaging its practical applications in biological systems. [ABSTRACT FROM AUTHOR]

Three-dimensional mechanical characteristics of rocks surrounding the stope of thick unconsolidated layers and thin bedrock were studied using the comprehensive research methods of similar conditions stope analogy, field measurement and numerical calculation. Draw the following conclusions: strata behaviors is related to the base-mining ratio, with the base-ming ratio increasing, strata behaviors is easing. According to the different base-mining ratio, stope can be classified as three types, including "having plate and shell" "having plate without shell" and "no plates no shell". For the "having plate and shell" type of stope,"stress shell" is first shelter of the stope, and rock beams or plates in fractured zone as second shelter of the stope, strata behaviors easing; For the "having plate without shell type of stop, rock beams or plates in fractured zone are first shelter of the stope, without upper bearing stress shell, if the structure of plates or beams is instability, strata behaviors of the stope is dramatic; For the "no plate no shell" type of stope,neither the structure of plates or beams nor "stress shell" shelter, alluvial soil load transfer on the hydraulic support directly, this is the important reason that the stope of small base-mining ratio is prone to happen the fully mechanized support crushing accidents. The overlying strata of " no plate no shell" type of stope according to rock vertical structure are divided into two zones, namely "caving zone" and "bending zone". And the mechanics mechanism of formation of three types of stope are analyzed. [ABSTRACT FROM AUTHOR]