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Your search keyword '"Li, Shu-Fen"' showing total 340 results
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340 results on '"Li, Shu-Fen"'

5. ACE2-independent infection of T lymphocytes by SARS-CoV-2

Author

Shen, Xu-Rui, Geng, Rong, Li, Qian, Chen, Ying, Li, Shu-Fen, Wang, Qi, Min, Juan, Yang, Yong, Li, Bei, Jiang, Ren-Di, Wang, Xi, Zheng, Xiao-Shuang, Zhu, Yan, Jia, Jing-Kun, Yang, Xing-Lou, Liu, Mei-Qin, Gong, Qian-Chun, Zhang, Yu-Lan, Guan, Zhen-Qiong, Li, Hui-Ling, Zheng, Zhen-Hua, Shi, Zheng-Li, Zhang, Hui-Lan, Peng, Ke, and Zhou, Peng

Published
2022
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7. Gibberellins regulate masculinization through the SpGAI‐SpSTM module in dioecious spinach.

Author

Zhang, Yu‐Lan, Wang, Li‐Ying, Yang, Yi, Zhao, Xu, Zhu, Hong‐Wei, You, Chen, Chen, Ning, Wei, Shuai‐Jie, Li, Shu‐Fen, and Gao, Wu‐Jun

Subjects
SPINACH, DIOECIOUS plants, TRANSCRIPTION factors, GIBBERELLINS, GIBBERELLIC acid, SEX hormones
Abstract

SUMMARY: The sex of dioecious plants is mainly determined by genetic factors, but it can also be converted by environmental cues such as exogenous phytohormones. Gibberellic acids (GAs) are well‐known inducers of flowering and sexual development, yet the pathway of gibberellin‐induced sex conversion in dioecious spinach (Spinacia oleracea L.) remains elusive. Based on sex detection before and after GA3 application using T11A and SSR19 molecular markers, we confirmed and elevated the masculinization effect of GA on a single female plant through exogenous applications of GA3, showing complete conversion and functional stamens. Silencing of GIBBERELLIC ACID INSENSITIVE (SpGAI), a single DELLA family protein that is a central GA signaling repressor, results in similar masculinization. We also show that SpGAI can physically interact with the spinach KNOX transcription factor SHOOT MERISTEMLESS (SpSTM), which is a homolog of the flower meristem identity regulator STM in Arabidopsis. The silencing of SpSTM also masculinized female flowers in spinach. Furthermore, SpSTM could directly bind the intron of SpPI to repress SpPI expression in developing female flowers. Overall, our results suggest that GA induces a female masculinization process through the SpGAI‐SpSTM‐SpPI regulatory module in spinach. These insights may help to clarify the molecular mechanism underlying the sex conversion system in dioecious plants while also elucidating the physiological basis for the generation of unisexual flowers so as to establish dioecy in plants. Significance Statement: Using environmental factors such as hormones to induce sex conversion is an important approach to studying dioecy sex differentiation, which is a special character closely related to flowering, reproduction, and fruiting. In our research, exogenous GA3 can induce functional masculinization of individual spinach, and the GA‐regulating pathway was established: GA‐SpGAI‐SpSTM‐SpPI. This study provides new ideas and theoretical support for studying the sex differentiation mechanisms of spinach and other dioecious plants. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Landscape and evolutionary dynamics of Helitron transposons in plant genomes as well as construction of online database HelDB.

Author

Li, Shu‐Fen, Zhang, Xin‐Yu, Yang, Long‐Long, Jia, Ke‐Li, Li, Jia‐Rong, Lan, Li‐Na, Zhang, Yu‐Lan, Li, Ning, Deng, Chuan‐Liang, and Gao, Wu‐Jun

Subjects
*ONLINE databases, *TRANSPOSONS, *PLANT genomes, *GENOME size, *DATABASES, *METAL ions, *EVOLUTIONARY algorithms
Abstract

Helitron transposons play an important role in host genome evolution due to their ability to capture genes and regulatory elements. In this study, we developed a pipeline to identify and annotate Helitrons systematically from 358 plant and 178 animal high‐quality genomes. All these data were organized into HelDB, a database where Helitrons can be explored with a user‐friendly Web interface and related software. Based on these data, further analysis showed that the number or the cumulative length of Helitrons is positively correlated with genome size. Helitrons had experienced two expansion periods in plants, with the first occurring 20–30 Ma and peaking at approximately 24 Ma. The second expansion occurred in the last 4 million years. The expansions might be due to stimulation of paleogeographic environment. Detailed investigation of gene capture by Helitrons in Brassicaceae and Solanaceae plants showed that the captured genes showed diverse functions. Interestingly, metal ion binding function was enriched in these captured genes in most species. This phenomenon might be due to the need for binding of divalent metal ions to the Rep domain required for Helitron transposition. This study improves our knowledge of the landscape and evolution of Helitron transposons in plants and paves a way for further functional studies of this kind of transposable element. [ABSTRACT FROM AUTHOR]

Published
2023
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24. Combining the Taguchi Method and Convolutional Neural Networks for Arrhythmia Classification by Using ECG Images with Single Heartbeats.

Author

Li, Shu-Fen, Huang, Mei-Ling, and Wu, Yan-Sheng

Subjects
*CONVOLUTIONAL neural networks, *TAGUCHI methods, *BIOMEDICAL engineering, *DEEP learning, *ELECTROCARDIOGRAPHY, *INTEROCEPTION, *ARRHYTHMIA
Abstract

In recent years, deep learning has been applied in numerous fields and has yielded excellent results. Convolutional neural networks (CNNs) have been used to analyze electrocardiography (ECG) data in biomedical engineering. This study combines the Taguchi method and CNNs for classifying ECG images from single heartbeats without feature extraction or signal conversion. All of the fifteen types (five classes) in the MIT-BIH Arrhythmia Dataset were included in this study. The classification accuracy achieved 96.79%, which is comparable to the state-of-the-art literature. The proposed model demonstrates effective and efficient performance in the identification of heartbeat diseases while minimizing misdiagnosis. [ABSTRACT FROM AUTHOR]

Published
2023
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