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IntroductionOsteosarcoma is a common bone malignant tumor in adolescents with high mortality and poor prognosis. At present, the progress of osteosarcoma and effective treatment strategies are not clear. This study provides a new potential target for the progression and treatment of osteosarcoma.MethodsThe relationship between lncRNA PRR7-AS1 and osteosarcoma was analyzed using the osteosarcoma databases and clinical sample testing. Cell function assays and tumor lung metastasis were employed to study the effects of PRR7-AS1 on tumorigenesis in vivo and in vitro. Potential downstream RNF2 of PRR7-AS1 was identified and explored using RNA pulldown and RIP. The GTRD and KnockTF database were used to predict the downstream target gene, MTUS1, and ChIP-qPCR experiments were used to verify the working mechanismy. Rescue experiments were utilized to confirm the role of MTUS1 in the pathway.ResultsDeep mining of osteosarcoma databases combined with clinical sample testing revealed a positive correlation between lncRNA PRR7-AS1 and osteosarcoma progression. Knockdown of PRR7-AS1 inhibited osteosarcoma cell proliferation and metastasis in vitro and in vivo. Mechanistically, RNA pulldown and RIP revealed that PRR7-AS1 may bind RNF2 to play a cancer-promoting role. ChIP-qPCR experiments were utilized to validate the working mechanism of the downstream target gene MTUS1. RNF2 inhibited the transcription of MTUS1 through histone H2A lysine 119 monoubiquitin. Rescue experiments confirmed MTUS1 as a downstream direct target of PRR7-AS1 and RNF2.DiscussionWe identified lncRNA PRR7-AS1 as an important oncogene in osteosarcoma progression, indicating that it may be a potential target for diagnosis and prognosis of osteosarcoma.

Background Objectives Methods Results Conclusions Anti‐p200 pemphigoid is a rare autoimmune subepidermal blistering disease. Although the phenomenon of epitope spreading has been reported to be common in anti‐p200 pemphigoid, the association between its clinical and immunoserological features has yet to be elucidated.Our aim was to compare the clinical and immunoserological characteristics of anti‐p200 pemphigoid patients with and without epitope spreading.We performed a retrospective cohort study encompassing 30 patients with anti‐p200 pemphigoid between January 2015 and December 2022. The clinical and immunoserological characteristics of anti‐p200 pemphigoid were analyzed using combined immunoserological assays.Epitope spreading was observed in 11 of 30 patients (36.7%) with anti‐p200 pemphigoid. Compared with patients in the non‐epitope spreading group, patients in the epitope spreading group showed more heterogeneous clinical presentations (P = 0.018), a higher proportion of mucosal involvement (P = 0.003), higher Bullous Pemphigoid Disease Area Index (BPDAI) scores for skin erosions/blisters (P = 0.018), mucosal erosions/blisters (P = 0.001), activity (P = 0.017) and total scores (P = 0.022), and required a higher initial dose of prednisone for disease control (P = 0.040).This study supported the idea that anti‐p200 pemphigoid was prone to epitope spreading. Anti‐p200 pemphigoid patients with epitope spreading are more likely to present heterogeneous clinical phenotypes, frequent mucosal involvement, and a more severe and recalcitrant disease course. [ABSTRACT FROM AUTHOR]

Background The mechanism of livedoid vasculopathy (LV) remains unknown. Objectives To investigate the association between coagulation factors and LV and to assess the efficacy and safety of rivaroxaban in the treatment of patients with LV. Methods From May 2019 to July 2022, 89 patients with LV and 35 healthy controls were included in a cross-sectional cohort to measure the levels of coagulation factors. In addition, 55 patients with LV treated with rivaroxaban were included in a treatment cohort to assess the complete remission rate of ulcers (n = 44) and retiform purpura (n = 11) within 12 weeks. Results In the cross-sectional cohort, the activities of coagulation factor X in patients with LV were significantly higher than those in healthy controls: median 110.5% [interquartile range (IQR) 97.5–127.0%] vs. 101.3% (IQR 91.6–115.6); P = 0.05. In addition, coagulation factor X activities in the progressive stage were higher than at the stable stage: median 111.6% (IQR 102.3–132.5) vs. 105.4% (IQR 92.9–118.8); P = 0.04. Moreover, coagulation factor X activities were higher at the progressive stage than at the stable stage in a subgroup of 20 patients with LV (P = 0.04). In the treatment cohort taking rivaroxaban, 91% (40/44) of patients with ulcers achieved complete remission within 12 weeks, and 73% (8/11) of patients with retiform purpura achieved complete remission within 12 weeks. Mild side-effects occurred in 25% of patients (14/55), including menorrhagia (n = 10), gingival bleeding (n = 3) and haemorrhage (n = 1). Conclusions Coagulation factor X was associated with the incidence and severity of LV in this study. In addition, rivaroxaban was an effective and safe treatment for ulcers and retiform purpura in people with LV. [ABSTRACT FROM AUTHOR]

Lithium‐ion batteries play an integral role in various aspects of daily life, yet there is a pressing need to enhance their safety and cycling stability. In this study, we have successfully developed a highly secure and flexible solid‐state polymer electrolyte (SPE) through the in situ polymerization of allyl acetoacetate (AAA) monomers. This SPE constructed an efficient Li+ transport channel inside and effectively improved the solid‐solid interface contact of solid‐state batteries to reduce interfacial impedance. Furthermore, it exhibited excellent thermal stability, an ionic conductivity of 3.82×10−4 S cm−1 at room temperature (RT), and a Li+ transport number (tLi+) of 0.66. The numerous oxygen vacancies on layered inorganic SiO2 created an excellent environment for TFSI− immobilization. Free Li+ migrated rapidly at the C=O equivalence site with the poly(allyl acetoacetate) (PAAA) matrix. Consequently, when cycled at 0.5C and RT, it displayed an initial discharge specific capacity of 140.6 mAh g−1 with a discharge specific capacity retention rate of 70 % even after 500 cycles. Similarly, when cycled at a higher rate of 5C, it demonstrated an initial discharge specific capacity of 132.3 mAh g−1 while maintaining excellent cycling stability. [ABSTRACT FROM AUTHOR]

Objective To explore the molecular mechanism by which secreted frizzy-related protein 2 (SFRP2) inhibits proliferation and migration of human colorectal cancer cell line SW480. Methods The expression of SFRP2 was detected by Western blot and RT-qPCR in human normal colonic epithelial cells HCoEpiC and colorectal cancer cell line SW480. The shSERP2 SW480 cell strain was constructed. Western blot and RT-qPCR were used to detect the expression of SFRP2, hypoxia inducible factor-1α (HIF-1α), and Twist in shNC SW480 and shSFRP2 SW480 cells. After shSFRP2 SW480 cells were stimulated by HIF-1α inhibitor (IDF-11774), CCK-8 assay detected cell proliferation and Transwell detected cell migration and invasion. Results The expression level of SFRP2 in SW480 cells was lower than that in HCoEpiC cells (P＜0.01). After SFRP2 knockout, the proliferation, migration and invasion ability of SW480 cells were significantly improved (P＜0.001). The expression of HIF-1α and Twist in SW480 cells was increased (P＜0.01). After HIF-1α inhibitor (IDF-11774) treatment of shSFRP2 SW480 cells, compared with the untreated group, the proliferation, migration and invasion ability of shSFRP2 SW480 cells were significantly decreased (P＜0.001). Conclusions SFRP2 exerts certain anticancer effects through regulation of the HIF-1α/Twist signaling axis.

Abstract Background Acetolactate synthase (ALS)-inhibiting herbicides from the chemical families of sulfonylureas and imidazolinones are used worldwide. However, drift or sprayer contamination from some sulfonylurea herbicides causes a high level of male sterility in cruciferous species, especially oilseed rape (OSR). In this paper, we evaluated the gametocidal effects of 27 ALS-inhibiting herbicides that were sprayed on OSR plants at the bolting stage. Results OSR anther development was very sensitive to sublethal exposure to most ALS-inhibiting herbicides. The application of 18 out of the 20 tested sulfonylureas (except ethametsulfuron and ethoxysulfuron), two imidazolinones (imazethapyr and imazamox), and one sulfonylamino-carbonyltriazolinone (flucarbazone-sodium) at suitable rates could induce male sterility. Eight of the herbicides, including chlorsulfuron (at application rates of 60–120 mg/ha), halosulfuron-methyl (300–600 mg/ha), sulfosulfuron (400–600 mg/ha), triflusulfuron-methyl (500–750 mg/ha), pyrazosulfuron-ethyl (150–225 mg/ha), nicosulfuron (200–300 mg/ha), imazethapyr (750–1125 mg/ha), and imazamox (400–800 mg/ha), could induce over 90% male sterility and over 60% relative outcrossed seed set in six cultivars with different origins. These eight chemicals could be used as new gametocides for hybrid seed production. This study also examined the possibility of external application of these gametocides on several unstable Polima cytoplasmic male sterile and thermosensitive genic male sterile lines. Although the outcrossed seed set of the treated lines was slightly reduced, the gametocide application significantly increased the seed purity of the resulting hybrid. Conclusion The finding of the gametocidal effects of most sulfonylureas and imidazolinones are of great importance for developing new functions for ALS-inhibiting herbicides. The application of gametocides will also greatly promote the safe utilization of environment-sensitive male sterility in hybrid seed production. Unexpectedly, the application of three triazolopyrimidines (florasulam, flumetsulam, and penoxsulam) and one pyrimidinylthiobenzoate (bispyribac-sodium) did not cause male sterility, although these herbicides obviously inhibited the activity of ALS and plant growth. This result suggests that inhibition of ALS activity does not always lead to male sterility in plants, and these gametocides may also inhibit other biological functions vital for microspore development.

To improve peanut yield under drip irrigation in Xinjiang, effect of potassium fertilizer application was investigated. Huayu 9610 (T1, a common cultivar in Xinjiang) and Heihua 5 (T2, a high yield cultivar) were used as materials at the Agricultural Meteorological Experiment Station in Wulanwusu, Xinjiang. Four potassium (K2O) fertilizer gradients were set as K0 (0 kg/hm²), K1 (150 kg/hm²), K2 (225 kg/hm²) and K3 (300 kg/hm²), and thus the potassium rates were at 15%, 40%, 30% and 15% at the seedling, pegging, podding and full fruit ripening stages respectively. Results showed that the main stem height and lateral branch length of Huayu 9610 and Heihua 5 changed parabolically with the increase of potassium application. The dry matter accumulation were the highest at K2 level, which were 84.37 g per plant and 89.37 g per plant, respectively. Compared with no potassium fertilizer, the main stem height and lateral branch length of K2 treatment increased by 17.91% and 16.56%, 7.4% and 12.31%, respectively. The number of nodules was the highest at podding stage, which was 42.0 per plant and 45.3 per plant respectively. The leaf area index increased with the increase of potassium fertilizer application, and reached the maximum at 90 days after emergence. The photosynthetic potential also changed in a parabolic shape with the increase of potassium fertilizer application rate, and reached the maximum value of 4.38 m2·d and 4.51 m2· d respectively after 75-90 days of K2 treatment. The fertilizer contribution rate and agronomic utilization rate of K2 treatment were the largest, which were 26.06% and 1.83 kg/kg (for Huayu 9610), 25.18% and 1.67 kg/kg (for Heihua 5), respectively, and the yields were 9628.38 kg/hm² and 10 368.35 kg/hm², respectively. The results showed that potassium application amount of 225 kg/hm² at K2 level could be used as reference for drip irrigation peanut in Xinjiang, China. [ABSTRACT FROM AUTHOR]

Traditional festival has always been the focus of academic research. As a custom activity of the Dragon Boat Festival that highlights the regional characteristics of Jingchu, Zelin Dryland Dragon Boat Festival has a long history and diverse parade ceremonies, which completely preserves the folk customs of the ancestors, such as offering sacrifices, exorcising evil spirits and praying for blessings. It deduces the original natural attributes of the Dragon Boat Festival and has multiple social functions such as inheriting cultural memory, maintaining country attachment and constructing narrative identity. In recent years, Zelin Dryland Dragon Boat Festival Ceremony has been selected into the national intangible cultural heritage list, and its cultural inheritance has attracted much attention. However, the transfer of power to capital has boosted the shift of cultural reproduction, and Zelin Dryland Dragon Boat is facing the dilemma of cultural inheritance, such as the gradual shift of folk customs, the fault of cultural intergenerational inheritance and the compression of festival cultural space. Based on this, we should repair local cultural memory, improve non-genetic inheritance system and expand multi-dimensional cultural space in order to realize the sustainable development of Zelin Dryland Dragon Boat. [ABSTRACT FROM AUTHOR]

Abstract Background Acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl (TBM) is an efficient gametocide that can cause rapeseed (Brassica napus L.) to become male sterile and outcrossing. To find the reason the TBM treatment leads to male sterility, an integrated study using cytological, physiological, and transcriptomic methods was conducted. Results Some temporary symptoms, including the discoloration of young leaves and a short halt of raceme elongation, were observed in the rapeseed plants exposed to TBM at an application rate of 1 μg per plant. Both chloroplasts in young leaves and plastids in anthers were deformed. TBM also reduced the leaf photosynthetic rate and the contents of chlorophyll, soluble sugar and pyruvate. Both the tapetal cells and uni-nucleate microspores in the treated plants showed large autophagic vacuoles, and the tissue degenerated quickly. A transcriptomic comparison with the control identified 200 upregulated and 163 downregulated differential expression genes in the small flower buds of the TBM treatment. The genes encoding functionally important proteins, including glucan endo-1,3-beta-glucosidase A6, QUARTET3 (QRT3), ARABIDOPSIS ANTHER 7 (ATA7), non-specific lipid-transfer protein LTP11 and LTP12, histone-lysine N-methyltransferase ATXR6, spermidine coumaroyl-CoA acyltransferase (SCT), and photosystem II reaction centre protein psbB, were downregulated by TBM exposure. Some important genes encoding autophagy-related protein ATG8a and metabolic detoxification related proteins, including DTX1, DTX6, DTX35, cytosolic sulfotransferase SOT12, and six members of glutathione S-transferase, were upregulated. In addition, several genes related to hormone stimulus, such as 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), ethylene-responsive factor ERF1A, ERF1, ERF71, CRF6, and RAP2-3, were also upregulated. The transcriptional regulation is in accordance with the functional abnormalities of pollen wall formation, lipid metabolism, chloroplast structure, ethylene generation, cell cycle, and tissue autophagy. Conclusion The results suggested that except for ALS, the metabolic pathways related to lipid metabolism, pollen exine formation, photosynthesis and hormone response are associated with male sterility induced by TBM. The results provide new insight into the molecular mechanisms of inducing male sterility by sulfonylurea.