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The experiment was conducted to study the effect of probiotics, acidifiers and enzyme preparations on performance, meat quality, serum biochemical indexes and antioxidant capacity of Dagu chickens. A total of 1 200 Dagu chickens were randomly divided into five groups, with four replicates in each group and 60 chickens in each replicate. The group A (control group) was basal diet, group B was basal diet + 0.3% probiotics, group C was basal diet + 0.3% probiotics + 0.2% acidifier, group D was basal diet + 0.3% probiotics + 100 g/t enzyme preparation, and group E was basal diet + 0.3% probiotics + 0.2% acidifier + 100 g/t enzyme preparation. The experiment lasted for 63 d. The results showed that the weight gain of Dagu chickens in group C, group D and group E was significantly higher than that in control group ( P<0.05), and feed to gain radio of Dagu chickens in group C, group D and group E was significantly lower than that in control group ( P<0.05). The breast muscle shear force of Dagu chickens in group C, group D, and group E was significantly lower than that in control group ( P<0.05), the leg muscle cooking loss of large-bone chickens in group E was significantly lower than that in control group ( P<0.05), and the leg muscle shear force of large-bone chickens in group D and group E was significantly lower than that in control group ( P<0.05). The serum total cholesterol and triglyceride content of Dagu chickens in group B, group C, group D and group E were significantly lower than those in control group ( P<0.05), the serum total protein content of Dagu chickens in group D and group E were significantly higher than those in control group ( P<0.05), and the serum alanine aminotransferase activity of Dagu chickens in group E was significantly lower than that in control group (P<0.05). Serum aspartate aminotransferase of Dagu chickens in group D and group E was significantly lower than that in control group ( P<0.05). The serum MDA content of large-bone chickens in group B, group C, group D and E was significantly lower than that in control group ( P<0.05), and the T-SOD activity and T-AOC were significantly higher than those in control group ( P<0.05). The serum GSH-Px activity of large-bone chickens in group C, group D and group E was significantly higher than that in control group ( P<0.05). The experiment indicates taht probiotics, acidifiers and enzyme preparations can improve growth performance, improve meat quality, reduce blood lipid, enhance liver function and enhance antioxidant capacity of Dagu chickens. [ABSTRACT FROM AUTHOR]

Aim Of Study: Fluorouracil drugs and irinotecan are commonly used in the treatment of colorectal cancer (CRC), but some patients have severe toxic side effects in the conventional dose. DPYD*2A/*5A/*9A and UGT1A1 * 6/*28 polymorphisms are related to the toxicity of fluorouracil drugs and irinotecan, respectively. Herein, we investigated the frequencies of DPYD*2A/*5A/*9A and UGT1A1 * 6/*28 genotypes in Chinese CRC patients.Materials and Methods: For this study, 117 CRC patients' tumor tissues were examined through sequencing technology of the first generation to explore the distribution of DPYD*2A/*5A/*9A and UGT1A1 * 6/*28 genotypes.Results: DPYD*2A G/G genotype accounted for 100%. DPYD*5A A/A, A/G, and G/G genotypes accounted for 48.2, 37.5, and 14.3%, respectively. DPYD*9A T/T and T/C genotypes accounted for 85.7 and 14.3%, respectively. UGT1A1 * 6 G/G, G/A, and A/A genotypes accounted for 74.6, 21.8, and 3.6%, respectively. UGT1A1 * 28 TA6/TA6, TA6/TA7, and TA7/TA7 genotypes accounted for 71.8, 27.3, and 0.9%, respectively. The genotypes of DPYD*2A/*5A/*9A and UGT1A1 * 6/*28 were not associated with patient's sex, age, and primary tumor sites. Our findings showed that: (i) almost 57.1% of Chinese CRC patients had at least one variant of DPYD*5A and DPYD*9A; (ii) nearly 37.3% of Chinese CRC patients had at least one variant of UGT1A1 * 6 and UGT1A1 * 28.Conclusion: It suggests that it is necessary for Chinese CRC patients to detect the genotypes of DPYD*5A/*9A and UGT1A1 * 6/*28 before treating with fluorouracil drugs and irinotecan. [ABSTRACT FROM AUTHOR]

Objectives: To study the correlation between neck circumference and body mass index and the value of neck circumference in identifying overweight and obesity in preschool children., Methods: The stratified cluster sampling method was used to recruit 3 719 children under 7 years from 10 kindergartens in Urumqi, China. General data were collected, and physical measurements were performed. A Pearson correlation analysis was used to evaluate the correlation between neck circumference and body mass index. The receiver operating characteristic (ROC) curve was used to assess the accuracy of neck circumference in identifying overweight/obesity. The Kappa consistency test was used to assess the consistency of neck circumference and body mass index in identifying overweight/obesity., Results: There was a positive correlation between neck circumference and body mass index in boys and girls of all ages ( r ≥0.50, P <0.001). According to body mass index as the criteria for overweight/obesity, the children were divided into an overweight/obesity group and a non-overweight/obesity group, and the analysis showed that the overweight/obesity group had a significantly larger neck circumference than the non-overweight/obesity group ( P <0.001). The ROC curve analysis showed that neck circumference had an area under the ROC curve of >0.7 in identifying overweight/obesity for boys and girls. The Kappa consistency test showed that the neck circumference and body mass index had a Kappa value of >0.40 in identifying overweight/obesity in boys and girls of all ages., Conclusions: Neck circumference is positively correlated to body mass index, and neck circumference can be used to identify overweight/obesity in preschool children.

Objective: To screening mutations of exons 15, 18 and 26 of sodium channel Nav1.7 (SCN9A) gene, and to assess its association with pain related to Parkinsonism., Methods: Respectively, 101 patients with primary Parkinson's disease (PD) and 104 similar-aged volunteers without PD were recruited from March, 2008 to January, 2011. Mutations of above 3 exons in SCN9A gene was detected with PCR and direct sequencing. For 100 patients with Parkinsonism, the pain was scored with a McGill pain rating scale. Statistical analysis was performed with SPSS., Results: The prevalence of pain in Parkinsonian was 57%. 43.86% patients with pain were males, and 56.14% were females. Based on Chaudhuri criteria, the pain symptoms may be classified as musculoskeletal pain (10.52%), radicular pain (10.52%), dyskinesis pain (54.38%), pain from akathisia and restlessness (14.04%), dyskinesis combined with radicular pain (5.26%), skeletal muscles pain and headache (1.75%), and arthralgia (3.50%). Two missense mutations were identified, which included 2794A/C (0.941/0.059) (rs12478318) (M932L) in exon 15 and 3448C/T (0.988/0.012) (rs6746030) (R1150W) in exon 18. The wild type A/C for the 2794 locus had a higher prevalence in PD patients with pain, but this was not statistically different. All of the 5 heterozygotes for 3448 (C/T) were found in Parkinsonian patients with pain. No homozygotes were found., Conclusion: The prevalence of pain was higher in Parkinsonian patients than general population, and the proportion of males to females was similar. More patients have suffered dyskinesis pain. A 3448 (C/T) mutation of SCN9A gene may be related to pathogenesis of pain in Parkinsonism.