1. The Potential Antioxidant Activity and Characterization of Bioactive Compounds of Stahlianthus involucratus.
- Author
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Li, Wengkun, Wu, BingMin, Wang, Yange, Lin, Ying, An, Lin, and Zhang, Guifang
- Subjects
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REVERSE transcriptase polymerase chain reaction , *PROTEINS , *MEDICINAL plants , *POLYPHENOLS , *FLAVONOIDS , *HIGH performance liquid chromatography , *PHENOLS , *ANTI-inflammatory agents , *METHANOL , *ANTIOXIDANTS , *ORGANIC compounds , *CELL physiology , *OXIDATIVE stress , *GENES , *MASS spectrometry , *PLANT extracts , *POLYMERASE chain reaction , *CHINESE medicine , *PHARMACODYNAMICS - Abstract
Stahlianthus involucratus (S. involucratus) has anti-inflammatory, antinociceptive, and antipyretic activities; however, there are no literature reports on its antioxidant capacity. This study presents a comparative assessment of the polyphenols contents, flavonoids contents, and antioxidant activity of the aqueous and methanol extracts of S. involucratus (ASI and MSI). Moreover, the expression of oxidative stress-related genes in H2O2-induced H9c2 cells pretreated with the MSI was measured by RT-qPCR, and furthermore, MSI were characterized by UHPLC-Q-Orbitrap-MS/MS. The results indicated that the MSI had higher antioxidant contents and antioxidant capacity, and MSI could inhibit H2O2-induced oxidative stress in H9c2 cells by activating the Nrf2/HO-1 pathway. UHPLC-Q-Orbitrap-MS/MS characterized 15 phenolic compounds from the MSI. In conclusion, S. involucratus has the potential antioxidant capacity. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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