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Lost Lagoon is a shallow freshwater-brackish water body located in Stanley Park, Vancouver. The lagoon is impacted by eutrophication, pollution, and the impact of invasive species. Precipitation causes runoff to carry contaminants from nearby Highway 99 into the biofiltration (BF) ponds in Lost Lagoon. BF ponds are designed to filter this runoff before it enters the lagoon using biological processes to remove pollutants, as well as slowing the flow of water to allow other contaminants to settle into sediments. This research was commissioned by the Stanley Park Ecological Society (SPES), which works closely with the Vancouver Park Board (VPB), local businesses, and community members to promote conservation and sustainable practices in the park. In August 2022, a fish die-off event prompted SPES to investigate the water quality and evaluate the efficiency of the BF ponds.