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7. IL-12-Overexpressed Nanoparticles Suppress the Proliferation of Melanoma Through Inducing ICD and Activating DC, CD8+ T, and CD4+ T Cells

Author

Shen,Huan-Huan, Peng,Jie-Fei, Wang,Ran-Ran, Wang,Ping-Yu, Zhang,Jia-Xiang, Sun,Hong-Fang, Liang,Yan, Li,Yan-Mei, Xue,Jiang-Nan, Li,You-Jie, Sun,Guang-Bin, Xie,Shu-Yang, Shen,Huan-Huan, Peng,Jie-Fei, Wang,Ran-Ran, Wang,Ping-Yu, Zhang,Jia-Xiang, Sun,Hong-Fang, Liang,Yan, Li,Yan-Mei, Xue,Jiang-Nan, Li,You-Jie, Sun,Guang-Bin, and Xie,Shu-Yang

Abstract

Huan-Huan Shen,1,2,* Jie-Fei Peng,1,3,* Ran-Ran Wang,4,* Ping-Yu Wang,1,5 Jia-Xiang Zhang,1 Hong-Fang Sun,1 Yan Liang,1 Yan-Mei Li,6 Jiang-Nan Xue,7 You-Jie Li,1 Guang-Bin Sun,1,* Shu-Yang Xie1,2,* 1Department of Biochemistry and Molecular Biology, Binzhou Medical University, YanTai, Shandong, 264003, People’s Republic of China; 2Shandong Laboratory of Advanced Materials and Green Manufacturing, Yantai, Shandong, 264000, People’s Republic of China; 3Department of Clinical Laboratory, the Affiliated Taian City Central Hospital of Qingdao University, Taian, 271000, People’s Republic of China; 4Institute of Rehabilitation Medicine, School of Rehabilitation Medicine, Binzhou Medical University, Yantai, Shandong, 264003, People’s Republic of China; 5Department of Epidemiology, Binzhou Medical University, YanTai, ShanDong, 264003, People’s Republic of China; 6Department of Immune Rheumatism, Yantaishan Hospital, Yantai, Shandong, 264000, People’s Republic of China; 7Department of Immunology, Binzhou Medical University, Yantai, Shandong, 264003, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shu-Yang Xie; Guang-Bin Sun, Department of Biochemistry and Molecular Biology, Binzhou Medical University, YanTai, Shandong, 264003, People’s Republic of China, Tel +86 535 6913010 ; +86 535 6913335, Fax +86 535 6913163, Email xieshuyang@bzmc.edu.cn; bingo@bzmc.edu.cnPurpose: The drug resistance and low response rates of immunotherapy limit its application. This study aimed to construct a new nanoparticle (CaCO3-polydopamine-polyethylenimine, CPP) to effectively deliver interleukin-12 (IL-12) and suppress cancer progress through immunotherapy.Methods: The size distribution of CPP and its zeta potential were measured using a Malvern Zetasizer Nano-ZS90. The morphology and electrophoresis tentative delay of CPP were analyzed using a JEM-1400 transmission electron microscope and an ul

Published
2024

8. Advancements in Stimulus-Responsive Co-Delivery Nanocarriers for Enhanced Cancer Immunotherapy

Author

Zhang,Meng-Ru, Fang,Lin-Lin, Guo,Yang, Wang,Qin, Li,You-Jie, Sun,Hong-Fang, Xie,Shu-Yang, Liang,Yan, Zhang,Meng-Ru, Fang,Lin-Lin, Guo,Yang, Wang,Qin, Li,You-Jie, Sun,Hong-Fang, Xie,Shu-Yang, and Liang,Yan

Abstract

Meng-Ru Zhang,1,2,* Lin-Lin Fang,3,* Yang Guo,1 Qin Wang,1 You-Jie Li,1 Hong-Fang Sun,1 Shu-Yang Xie,1 Yan Liang1 1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Binzhou Medical University, YanTai, ShanDong, 264003, People’s Republic of China; 2Department of Clinical Medicine, Binzhou Medical University, YanTai, ShanDong, 264003, People’s Republic of China; 3RemeGen Co., Ltd, YanTai, ShanDong, 264000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan Liang; Shu-Yang Xie, Department of Biochemistry and Molecular Biology, School of Basic Medicine, Binzhou Medical University, YanTai, ShanDong, 264003, People’s Republic of China, Tel/Fax +86 535 6913335 ; +86 535 6913166, Email 18766567201@163.com; xieshuyang@bzmc.edu.cnAbstract: Cancer immunotherapy has emerged as a novel therapeutic approach against tumors, with immune checkpoint inhibitors (ICIs) making significant clinical practice. The traditional ICIs, PD-1 and PD-L1, augment the cytotoxic function of T cells through the inhibition of tumor immune evasion pathways, ultimately leading to the initiation of an antitumor immune response. However, the clinical implementation of ICIs encounters obstacles stemming from the existence of an immunosuppressive tumor microenvironment and inadequate infiltration of CD8+T cells. Considerable attention has been directed towards advancing immunogenic cell death (ICD) as a potential solution to counteract tumor cell infiltration and the immunosuppressive tumor microenvironment. This approach holds promise in transforming “cold” tumors into “hot” tumors that exhibit responsiveness to antitumor. By combining ICD with ICIs, a synergistic immune response against tumors can be achieved. However, the combination of ICD inducers and PD-1/PD-L1 inhibitors is hindered by issues such as poor targeting and uncontrolled drug release. An advantageous solution presented by stimulus-re

Published
2024

10. IL-12-Overexpressed Nanoparticles Suppress the Proliferation of Melanoma Through Inducing ICD and Activating DC, CD8+ T, and CD4+ T Cells

Author

Shen, Huan-Huan, primary, Peng, Jie-Fei, additional, Wang, Ran-Ran, additional, Wang, Ping-Yu, additional, Zhang, Jia-Xiang, additional, Sun, Hong-Fang, additional, Liang, Yan, additional, Li, Yan-Mei, additional, Xue, Jiang-Nan, additional, Li, You-Jie, additional, Sun, Guang-Bin, additional, and Xie, Shu-Yang, additional

Published
2024
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11. Novel circular RNA hsa_circ_0036683 suppresses proliferation and migration by mediating the miR‐4664‐3p/CDK2AP2 axis in non‐small cell lung cancer.

Author

Liu, Rui, Zhang, Han, Xin, Jiaxuan, Xie, Shu‐yang, Jiao, Fei, Li, You‐Jie, Chu, Meng‐yuan, Qiu, Junming, and Yan, Yun‐fei

Subjects
THERAPEUTIC use of antineoplastic agents, WOUND healing, RESEARCH funding, CIRCULAR RNA, MICRORNA, CELL proliferation, ANTINEOPLASTIC agents, CELL motility, REVERSE transcriptase polymerase chain reaction, GENE expression, GASTROINTESTINAL hormones, CYTOMETRY, WESTERN immunoblotting, NEUROPEPTIDES, LUNG cancer, SEQUENCE analysis, PRECIPITIN tests, PHARMACODYNAMICS
Abstract

Background: The aim of the present study was to investigate the function of novel circular RNA hsa_circ_0036683 (circ‐36683) in non‐small cell lung cancer (NSCLC). Methods: RNA sequencing was used to screen out differentially expressed miRNAs. Expression levels of miR‐4664‐3p and circ‐36683 were evaluated in lung carcinoma cells and tissues by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The effects of miR‐4664‐3p and circ‐36683 on proliferation and migration were assessed using cell counting kit‐8 (CCK‐8), wound healing and transwell migration assays and xenograft experiments. The targeting relationship of circ‐36683/miR‐4664‐3p/CDK2AP2 was assessed by luciferase reporter assays, western blot, qRT‐PCR and argonaute2‐RNA immunoprecipitation (AGO2 RIP). Co‐immunoprecipitation (Co‐IP), 5‐ethynyl‐2′‐deoxyuridine (EdU) staining and CCK‐8 were used to validate the indispensable role of CDK2AP2 in suppressing cell proliferation as a result of CDK2AP1 overexpression. Results: By RNA sequencing, miR‐4664‐3p was screened out as an abnormally elevated miRNA in NSCLC tissues. Transfection of miR‐4664‐3p could promote cell proliferation, migration and xenograft tumor growth. As a target of miR‐4664‐3p, CDK2AP2 expression was downregulated by miR‐4664‐3p transfection and CDK2AP2 overexpression could abolish the proliferation promotion resulting from miR‐4664‐3p elevation. Circ‐36683, derived from back splicing of ABHD2 pre‐mRNA, was attenuated in NSCLC tissue and identified as a sponge of miR‐4664‐3p. The functional study revealed that circ‐36683 overexpression suppressed cell proliferation, migration and resulted in G0/G1 phase arrest. More importantly, the antioncogenic function of circ‐36683 was largely dependent on the miR‐4664‐3p/CDK2AP2 axis, through which circ‐36683 could upregulate the expression of p53/p21/p27 and downregulate the expression of CDK2/cyclin E1. Conclusion: The present study revealed the antioncogenic role of circ‐36683 in suppressing cell proliferation and migration and highlighted that targeting the circ‐36683/miR‐4664‐3p/CDK2AP2 axis is a promising strategy for the intervention of NSCLC. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Dual Stimuli-Responsive Micelles for Imaging-Guided Mitochondrion-Targeted Photothermal/Photodynamic/Chemo Combination Therapy-Induced Immunogenic Cell Death

Author

Liang,Yan, Wang,Ping-Yu, Liu,Ze-Yun, Sun,Hong-Fang, Wang,Qin, Sun,Guang-Bin, Zhang,Xia, Li,You-Jie, Xie,Shu-Yang, Liang,Yan, Wang,Ping-Yu, Liu,Ze-Yun, Sun,Hong-Fang, Wang,Qin, Sun,Guang-Bin, Zhang,Xia, Li,You-Jie, and Xie,Shu-Yang

Abstract

Yan Liang,1,2 Ping-Yu Wang,1 Ze-Yun Liu,3 Hong-Fang Sun,1 Qin Wang,1 Guang-Bin Sun,1 Xia Zhang,1 You-Jie Li,1 Shu-Yang Xie1,2 1Department of Biochemistry and Molecular Biology, Binzhou Medical University, YanTai, ShanDong, 264003, People’s Republic of China; 2Department of Physiology and Pathophysiology, School of Basic Medicine, Qingdao University, QingDao, ShanDong, 266071, People’s Republic of China; 3School of International Studies, Binzhou Medical University, YanTai, ShanDong, 264003, People’s Republic of ChinaCorrespondence: Shu-Yang Xie; You-Jie Li, Department of Biochemistry and Molecular Biology, Binzhou Medical University, YanTai, ShanDong, 264003, People’s Republic of China, Tel/Fax +86 535 6913166 ; Tel/Fax +86 535 6913335, Email shuyangxie@aliyun.com; youjie1979@163.comIntroduction: As the special modality of cell death, immunogenic cell death (ICD) could activate immune response. Phototherapy in combination with chemotherapy (CT) is a particularly efficient tumor ICD inducing method that could overcome the defects of monotherapies.Methods: In this study, new dual stimuli-responsive micelles were designed and prepared for imaging-guided mitochondrion-targeted photothermal/photodynamic/CT combination therapy through inducing ICD. A dual-sensitive methoxy-polyethylene glycol-SS-poly(L-γ-glutamylglutamine)-SS-IR780 (mPEG-SS-PGG-SS-IR780) polymer was synthesized by grafting IR780 with biodegradable di-carboxyl PGG as the backbone, and mPEG-SS-PGG-SS-IR780/paclitaxel micelles (mPEG-SS-PGG-SS-IR780/PTXL MCs) were synthesized by encapsulating PTXL in the hydrophobic core.Results: In-vivo and -vitro results demonstrated that the three-mode combination micelles inhibited tumor growth and enhanced the therapeutic efficacy of immunotherapy. The dual stimuli-responsive mPEG-SS-PGG-SS-IR780/PTXL MCs were able to facilitate tumor cell endocytosis of nanoparticles. They were also capable of promoting micelles disintegration and accelerating PTXL release. The

Published
2023

19. A Mathematic Mobile Cloud Computing System

Author

Liang, Tyng-Yeu, Li, You-Jie, He, Ga-Jin, Liao, Jian-Cheng, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Kobsa, Alfred, Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Weikum, Gerhard, Series editor, Hsu, Robert C.-H., editor, and Wang, Shangguang, editor

Published
2014
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25. Delivery of miR‐3529‐3p using MnO2‐SiO2‐APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A.

Author

Zhang, Ying, Wang, Ran‐Ran, Liu, Rui, Xie, Shu‐Yang, Jiao, Fei, Li, You‐Jie, Xin, Jiaxuan, Zhang, Han, Wang, Zhenbo, and Yan, Yun‐Fei

Subjects
LUNG cancer treatment, ADENOCARCINOMA, LUNG cancer, DISEASE progression, DRUG delivery systems, FLOW cytometry, XENOGRAFTS, PHOTOTHERAPY, ANIMAL experimentation, CYTOMETRY, WESTERN immunoblotting, MICRORNA, APOPTOSIS, METASTASIS, CELL motility, TUMOR suppressor genes, GENE expression profiling, CELL proliferation, PATHOLOGIC neovascularization, RESEARCH funding, COMBINED modality therapy, POLYMERASE chain reaction, REACTIVE oxygen species, BODY temperature regulation, NANOPARTICLES, MICE, HYPOXEMIA
Abstract

Background: The present study aimed to investigate the function of miR‐3529‐3p in lung adenocarcinoma and MnO2‐SiO2‐APTES (MSA) as a promising multifunctional delivery agent for lung adenocarcinoma therapy. Methods: Expression levels of miR‐3529‐3p were evaluated in lung carcinoma cells and tissues by qRT‐PCR. The effects of miR‐3529‐3p on apoptosis, proliferation, metastasis and neovascularization were assessed by CCK‐8, FACS, transwell and wound healing assays, tube formation and xenografts experiments. Luciferase reporter assays, western blot, qRT‐PCR and mitochondrial complex assay were used to determine the targeting relationship between miR‐3529‐3p and hypoxia‐inducible gene domain family member 1A (HIGD1A). MSA was fabricated using MnO2 nanoflowers, and its heating curves, temperature curves, IC50, and delivery efficiency were examined. The hypoxia and reactive oxygen species (ROS) production was investigated by nitro reductase probing, DCFH‐DA staining and FACS. Results: MiR‐3529‐3p expression was reduced in lung carcinoma tissues and cells. Transfection of miR‐3529‐3p could promote apoptosis and suppress cell proliferation, migration and angiogenesis. As a target of miR‐3529‐3p, HIGD1A expression was downregulated, through which miR‐3529‐3p could disrupt the activities of complexes III and IV of the respiratory chain. The multifunctional nanoparticle MSA could not only efficiently deliver miR‐3529‐3p into cells, but also enhance the antitumor function of miR‐3529‐3p. The underlying mechanism may be that MSA alleviates hypoxia and has synergistic effects in cellular ROS promotion with miR‐3529‐3p. Conclusions: Our results establish the antioncogenic role of miR‐3529‐3p, and demonstrate that miR‐3529‐3p delivered by MSA has enhanced tumor suppressive effects, probably through elevating ROS production and thermogenesis. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Dual-Targeted Fe3O4@MnO2 Nanoflowers for Magnetic Resonance Imaging-Guided Photothermal-Enhanced Chemodynamic/Chemotherapy for Tumor

Author

Xiao, Hui-Fang, primary, Yu, Hui, additional, Wang, De-Qiang, additional, Liu, Xin-Zheng, additional, Sun, Wan-Ru, additional, Li, You-Jie, additional, Sun, Guang-Bin, additional, Liang, Yan, additional, Sun, Hong-Fang, additional, Wang, Ping-Yu, additional, Xie, Shu-Yang, additional, and Wang, Ran-Ran, additional

Published
2022
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28. TRIB2 Promotes Cell Aerobic Glycolysis and Proliferation of Lung Cancer by Regulating PKM2

Author

Liu, Yuan-Rong, primary, Song, Dan-Dan, additional, Liang, Dong-Min, additional, Li, You-Jie, additional, Yan, Yun-Fei, additional, Sun, Hong-Fang, additional, Zhang, Mei-Ling, additional, Hu, Jin-Xia, additional, Zhao, Yu-Long, additional, Liang, Yan, additional, Li, Yan-Mei, additional, Yang, Zhen, additional, Zhang, Shu-Cui, additional, Zhang, QunYe, additional, Wang, Ran-Ran, additional, Zheng, Hou-Feng, additional, Wang, Ping-Yu, additional, and Xie, Shu-Yang, additional

Published
2022
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34. miR-4293 Upregulates lncRNA WFDC21P by Directly Suppressing mRNA-Decapping Enzyme 2 to Promote Lung Carcinoma Proliferation

Author

Zhang, Qian, primary, Yan, Yun-Fei, additional, Lv, Qing, additional, Li, You-Jie, additional, Wang, Ran-Ran, additional, Sun, Guang-Bin, additional, Pan, Li, additional, Hu, Jin-Xia, additional, Xie, Ning, additional, Zhang, Can, additional, Tian, Bao-Cheng, additional, Jiao, Fei, additional, Xu, Sen, additional, Wang, Ping-Yu, additional, and Xie, Shu-Yang, additional

Published
2020
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36. Effect of microcin C7 on growth performance, serum and hepatopancreas biochemical indexes of Procambarus clarkii.

Author

YUAN Zhi-wen, LI You-jie, LI Yong-an, LI Shu-yao, and WANG Zi-rui

Subjects
*PROCAMBARUS clarkii, *ASPARTATE aminotransferase, *ALANINE aminotransferase, *WEIGHT gain, *SURVIVAL rate
Abstract

The experiment was to study the effect of microcin C7 supplementation on growth performance, serum and hepatopancreas biochemical indexes of Procambarus clarkii. A total of 500 Procambarus clarkii with an initial average weight of (10.19±0.05) g were randomly divided into five groups, with five replicates in each group and 20 Procambarus clarkii in each replicate. Procambarus clarkii were fed with a basic diet with 0, 2.5, 5.0, 7.5 and 10.0 mg/kg microcin C7 supplementation, respectively. The experiment lasted for 8 w. The results showed that the final weight, weight gain rate and specific growth rate in 7.5 mg/kg group were the highest, which were significantly higher than those in 2.5 mg/kg group (P<0.05), but there was no significant difference between 7.5 mg/kg group and other groups (P>0.05). The survival rate of 7.5 mg/kg group was significantly higher than that of 10.0 mg/kg group (P<0.05). Serum alanine aminotransferase (ALT) activity in 10.0 mg/kg group was 46.44% lower than that in control group (P<0.05), and serum aspartate aminotransferase (AST) activity in 7.5 mg/kg group was 44.54% lower than that in control group (P<0.05). The hepatopancreas total protein (TP) content in 5.0 mg/kg group was significantly higher than that in control group, 7.5 mg/kg group and 10.0 mg/kg group (P<0.05). The experiment indicates that adding microcin C7 can improve the growth performance and liver function of Procambarus clarkii, and the effect is the best in 7.5 mg/kg group. [ABSTRACT FROM AUTHOR]

Published
2022
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