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Zearalenone (ZEN) is a common fungal toxin with reproductive toxicity in various grains. It poses a serious threat to ovine and other animal husbandry industries, as well as human reproductive health. Therefore, investigating the mechanism of toxicity and screening antagonistic drugs are of great importance. In this study, based on the natural compound library and previous Smart-seq2 results, antioxidant and anti-apoptotic drugs were selected for screening as potential antagonistic drugs. Three natural plant compounds (oxysophoridine, rutin, and phellodendrine) were screened for their ability to counteract the reproductive toxicity of ZEN on ovine oocytes in vitro using quantitative polymerase chain reaction (qPCR) and reactive oxygen species detection. The compounds exhibited varying pharmacological effects, notably impacting the expression of antioxidant (GPX, SOD1, and SOD2), autophagic (ATG3, ULK2, and LC3), and apoptotic (CAS3, CAS8, and CAS9) genes. Oxysophoridine promoted GPX, SOD1, ULK2, and LC3 expression, while inhibiting CAS3 and CAS8 expression. Rutin promoted SOD2 and ATG3 expression, and inhibited CAS3 and CAS9 expression. Phellodendrine promoted SOD2 and ATG3 expression, and inhibited CAS9 expression. However, all compounds promoted the expression of genes related to cell cycle, spindle checkpoint, oocyte maturation, and cumulus expansion factors. Although the three drugs had different regulatory mechanisms in enhancing antioxidant capacity, enhancing autophagy, and inhibiting cell apoptosis, they all maintained a stable intracellular environment and a normal cell cycle, promoted oocyte maturation and release of cumulus expansion factors, and, ultimately, counteracted ZEN reproductive toxicity to promote the in vitro maturation of ovine oocytes. This study identified three drugs that antagonize the reproductive toxicity of ZEN on ovine oocytes, and compared their mechanisms of action, providing data support and a theoretical basis for their subsequent application in the ovine breeding industry, reducing losses in the breeding industry, screening of ZEN reproductive toxicity antagonists and various toxin antagonists, improving the study of ZEN reproductive toxicity mechanisms, and even protection of human reproductive health. [ABSTRACT FROM AUTHOR]

This paper studied the problem of ice and snow pollutants identification and freezing temperature detection under winter runway operation, and designed an active detection system for runway ice and snow pollutants and freezing temperature. The temperature change trend during the liquid freezing process was researched, and the freezing temperature detection model based on sequence segmented linear fitting and inflection point identification was proposed in combination with active cooling technology. The differences in dielectric properties of runway snow and ice cover are studied, and a multi-frequency detection-based forked-finger planar capacitive sensor was identified for snow and ice pollutants detection considering practical application scenarios. A finite element simulation model of the forked-finger planar capacitive sensor was established, to optimize the structural parameters of the sensor and verify the feasibility of the capacitor sensor. Finally, the detection device and control system were designed and fabricated, and built an experimental platform for system test. The field experimental results showed that the system has good reliability and stability, and the error of freezing temperature detection model is less than 0.3 °C. At the same time, it can identify three types of pollutants, including water, ice and ice–water mixture, with an accuracy of 89%. [ABSTRACT FROM AUTHOR]