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C14H14AsNO5, monoclinic, P21 (no. 4), a = 8.5830(5) Å, b = 7.0799(5) Å, c = 11.6011(7) Å, β = 100.059(2)∘, V = 694.13(8) Å3, Z = 2, R gt(F) = 0.0288, wR ref(F 2) = 0.0603, T = 150.0 K.

As an important part of strategic emerging industries, the new-generation information technology plays a major leading role in the overall and long-term development of the economy and society. The new-generation information technology industry is also the cornerstone for cultivating new quality productivity, and is an important starting point for opening up new fields and tracks for development and for shaping new momentum and advantages. Based on the implications of the new-generation information technology industry, this study sorts out the global development trend of the new-generation information technology industry, summarizes the current status and development trend of the industry in China, and analyzes the opportunities and challenges faced by the industry. Moreover, focusing on the requirements of high-quality development of the new-generation information technology industry, the study proposes the following countermeasures and suggestions: (1) improving the integrated strategic system and capabilities while adhering to a system engineering thinking; (2) strengthening the research of key core technologies to strengthen the basic capacity of the industry; (3) promoting the integrated and clustered development of industries, highlighting enterprises as the main body; (4) constructing an open innovation ecosystem based on scenario-driven innovation; and (5) promoting the coordinated development of production, education, research, application, and funds to cultivate a high-level talent team.

To determine whether ultrasound (US) features of breast cancer are associated with Breast Imaging and Reporting Data System molecular subtype, histologic grade, and hormone receptor status as well as to assess the predictive value of these features. Retrospective analysis of the medical records of 220 consecutive patients with invasive breast cancer was reviewed according to the PIK3CA-mutated molecular tumor subtype. US findings of all patients were analyzed. Breast tumors harboring a PIK3CA-mutation were large and exhibited liquefied necrosis and posterior echo attenuation in the nodule. Moreover, such tumors were lobulated and calcified. The aspect ratio of the PIK3CA-mutant was more likely >1. The average nodule elasticity (7.479 ± 0.993 m/s) was measured using US shear wave elastography. Microcalcification was easier to detect inside the nodule using a fluorescence technique. Measurement of the nodule blood flow spectrum showed that the internal blood flow resistance index of nodules was lower than that of other types of breast cancer. The sonographic features of PIK3CA-mutated breast cancers were strongly associated with extensive and liquefied necrosis. The ability to predict molecular subtypes, particularly using US to detect the triple-negative subtype, may play an important role in early management and treatment.

This paper summarized the effect of prone position ventilation and nursing management for a patient with respiratory distress syndrome caused by COVID-19. The compressive nursing assessment, airway management and tube fixation were conducted before using prone position ventilation for therapy. After implementation of prone position ventilation for therapy, efforts had been made to enhance the vital signs monitoring, tube maintenance, airway management, nutrition support and prevention of pressure injuries. (本文总结1例新型冠状病毒感染致呼吸窘迫综合征患者俯卧位通气法的应用效果及护理体会。操作前系统评估患者和完善物品准备, 保持气道通畅和妥善固定管道; 操作后密切监测患者生命体征, 加强导管维护、呼吸道护理和营养支持, 积极预防压力性损伤。)

This paper analyzes the engineering characteristics of loess and the factors affecting wet subsidence, briefly outlines the application effect of PP modular cisterns, and combines the two to design a PP modular cistern based on rainwater harvesting. Combined with the analysis of the pre-engineering conditions of the storage tank, propose the treatment method for wet subsidence loess foundations. Design the structure of the storage tank by determining the volume of the tank by comparing the difference between the inlet and outlet flow during the storage time integral. Simulate the modeling of storm floods and the process of emptying stormwater regulating ponds. Evaluate the geologic conditions for the construction of the regulating pond project. Compare the original investigation report with the results of this paper’s test of the engineering foundation’s wet subsidence coefficient. Set different thicknesses of bedding layers and calculate the settlement of the center of the pool under different humidity levels of the loess layer. Simulate the 2.3-hour rainstorm process, analyze the storage volume of the storage pond during the period, and calculate the cumulative storage volume of the storage pond. This project’s foundation loess wet subsidence coefficient is medium, and the actual additional pressure is much smaller than the starting pressure of wet subsidence. It is appropriate to use the soil bedding program (1.6m plain soil bedding layer and then rammed 0.4m thick 2:8 gray soil, with PP module seepage control). Combined with the local hydrological conditions, it is known that the peak present time of the peak outflow from the regulating pond TP =85min, and the volume of the regulating pond is 22543.47 m3.

To strengthen the comprehensive literacy of college students and have a correct aesthetic concept, the cultivation of innovative talents in modern college aesthetic education under big data is proposed. The mixed-filtered college aesthetic training data were used to calculate the prediction function, loss function, and regression parameters using a logistic regression model. The training sample probabilities are derived from the maximum likelihood function to predict the unmade scores in the teacher’s scoring matrix, which is trained to fit the original scoring matrix. The behavior of each student is divided into a corresponding label, and the aesthetic education courses with historical preferences of students in the same category are recommended to the target students in that category. By iteratively updating the values of each cluster center one by one, the data with high similarity are clustered into one class, and the distances of all the cluster centers are recalculated to alleviate the data sparsity problem and scalability problem of logistic regression. The information of the training set is used as input data, and the numerical type indicators are used as the basis for judgment to obtain the satisfaction situation of students with the recommended results, and the indicators for visually evaluating the gap between the predicted and real ratings of the system and the accurate and complete data on the cultivation of innovative talents in modern university aesthetic education are counted. The analysis results show that under the big data technology, the physical education courses in colleges and universities carry out the education of the concept of beauty, the innovative form is better, and the percentage of the concept of strength and beauty reaches 69%, strengthening the ability of college students to feel beauty.

Objective To observe the osteoprotective effect of equol (Eq) on postmenopausal rats with nonalcoholic fatty liver disease (NAFLD), and to explore its underlying mechanism. Methods Forty-eight 6-week-old female Sprague-Dawley (SD) rats were randomly divided into control group, model group, low-, middle- and high-dose Eq groups [20, 40 and 80 mg/(kg·d)], and estradiol intervention group [E2, 0.25 mg/(kg·d)], with 8 rats in each group. The control group was given sham operation and normal diet. The other groups were given ovariectomy (OVX) combined with high-fat diet. After 16 weeks, the body weight and length of rats were measured, BMI and Lee's index were calculated, and the changes of bone mineral density (BMD) and bone microstructure of rats were detected. After anesthesia, the rats were killed to detect the levels of serum estrogen, TNF-α and IL-6. The liver and femur tissues were observed for morphological changes, and the expression of insulin-like growth factor-1 (IGF-1) in the liver and bone was detected by Western blotting and qRT-PCR. Results In the model group, BMI and Lee's index were increased significantly, the liver tissue was obviously injured, the estrogen level and BMD were obvioiusly decreased, and the bone microstructure was seriously damaged, suggesting that postmenopausal NAFLD was complicated with obvious bone injury. The treatment of Eq or E2 increased the level of serum estrogen and BMD, decreased BMI, Lee's index and serum TNF-α and IL-6 levels, attenuated steatosis and lipid accumulation in bone microstructure, and enhanced the expression of IGF-1 at protein and mRNA level in the liver and bone. Conclusion Eq can improve the bone injury of postmenopausal NAFLD rats and shows a bone protective effect. The mechanism may partially be related to Eq promoting the expression of IGF-1 and reducing inflammation. x

A mathematical model has been suggested for the numerical study of blood flow in a vessel due to the pumping action of the heart. Blood is assumed to contain some impurities in the form of chemically reactive species (undergoing a first-order irreversible reaction) and, being a hybrid nanofluid, also contains the nano-sized solid particles, thus forming a homogeneous mixture which is subjected to a pressure gradient (of trigonometric nature) in the horizontal direction. Human vessel is subjected to a transverse magnetic field and is presumed to be filled with plaque which is considered as a porous medium, and is mathematically modeled by applying the Darcy–Forchheimer theory. The nonlinear nature of the governing equations steered toward the decision of using the numerical approach to obtain the solution of the governing system, which led to the discovery of a linear concentration variation across the vessel at higher values of the Reynolds number. Finally, a 38% rise in the heat transfer has been noted due to the presence of solid particles in the human blood.

Objective To explore the effect and mechanism of equol(Eq) on the proliferation and apoptosis of osteoblasts in high-glucose and high-fat environment. Methods High-glucose and high-fat model of ROS17/2.8 osteoblasts was established, and cells were divided into the control group (C group) and the model group (M group), the Eq-7 group (1×10-7mol/L Eq), the Eq-6 group(1×10-6mol/L Eq) and the Eq-5 group(10-5mol/L Eq). CCK-8 assay and HE staining was used to detect the viability and morphology of osteoblasts; Flow cytometry was used to test the apoptosis and cell cycle; Western blotting was used to detect the phosphorylation of PI3K/AKT signaling pathway and expression of associated proteins of osteoblasts. PI3K inhibitor was added to confirm the role of PI3K/AKT signaling pathway in the osteoprotective effect of Eq. Results Compared with the C group, the cell structure of the M group was significantly damaged, the cell survival rate, proportion of S phase of cell cycle, the degree of phosphorylation of PI3K/AKT pathway, the expression of Cyclin D1 and Bcl-2 in the M group decreased, while cell inhibition rate, cell apoptosis, proportion of G1 phase of cell cycle, the expression of Bax and cleaved caspase-3 of the M group increased (P < 0.05); Compared with the M group, the Eq groups had increased cell survival rate, decreased cell inhibition rate, enhanced fraction of osteoblast in the S phase and decreased osteoblast apoptosis rate(P < 0.05). Eq treatment enhanced the phosphorylation of PI3K/AKT pathway of osteoblasts and increased expression level of Cyclin D1 and Bcl-2 protein and decreased expression of Bax and cleaved caspase-3 (P < 0.05). The intervention effect of Eq enhanced with the increase of concentration of Eq. However, this regulatory effect of Eq almost disappeared after co-administration of PI3K inhibitor. Conclusion Eq can reduce the apoptosis and promote the proliferation of osteoblasts in the high-glucose and high-fat environment by activating the PI3K/AKT pathway.

Objective To observe the effect of equol (Eq) intervention on sodium oleate (NaOL)-induced steatosis in HepG2 cells and study its mechanism of action. Methods HepG2 cells were treated with different concentrations of NaOL (0, 0.12, 0.24, 0.36, 0.48, 0.60 and 0.72 mmol/L) for 48 h to induce cell steatosis. Oil red O staining was used to observe the accumulation of intracellular lipid droplets, and triglyceride (TG) content was measured. CCK-8 assay was employed to determine the optimal concentration of NaOL in the inducement of hepatic steatosis and the concentration range for Eq intervention. Then HepG2 cells were randomly divided into 6 groups: control group, NaOL group, NaOL+low-, medium- and high-dose Eq groups (10-7, 10-6, and 10-5 mol/L), NaOL+estradiol intervention group (E2, 10-7 mol/L). After Eq intervention for 48 h, cellular TG content was detected, and Oil red O staining was adopted to observe the accumulation of intracellular lipid droplets, and the expression of SREBP-1c, FAS, PPARα and CPT-1A at mRNA and protein levels was detected by qRT-PCR and Western blotting, respectively. Results NaOL of 0.24 mmol/L showed no significant effect on the viability of HepG2 cells, but significantly increased intracellular TG content and accumulated a large number of lipid droplets. Compared with the NaOL group, Eq or E2 intervention reduced the intracellular lipid droplet accumulation in HepG2 cells induced by NaOL, significantly decreased the intracellular TG content (P

Objective To analyze the effects of S-Equol on glucose and lipid metabolism in rats with type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD). Methods Fifty SD rats aged 6 weeks were randomly divided into the control group (n=10) and the modeling group (n=40) after 1 week's adaptive feed. The modeling group was given high-fat and high-sugar diet combined with streptozotocin (STZ) intrabdominal injection to construct T2DM model. Rats whose fasting blood-glucose (FBG) level was greater than 11.1 mmol/L were randomly divided into the 4 groups: the model group, the S-Equol low-dose group(20 mg·kg-1·d-1), the S-Equol medium-dose group(40 mg·kg-1·d-1)and the S-Equol high-dose group(80 mg·kg-1·d-1). After 12 weeks of intervention, the level of serum total cholesterol (TC), triglyceride (TG), FBG, high density lipoprotein (HDL), low density lipoprotein (LDL), total bilirubin (TBIL), direct bilirubin (DBIL), total protein (TP), albumin (Alb) and globulin (Glb) were detected. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by ELISA. Peroxisome proliferator-activated receptor gamma (PPARγ), acetyl-CoA carboxylase (ACC), sterol regulatory element binding protein-1 (SREBP-1) and fatty acid synthetase (Fasn) expression levels related to lipid metabolism were detected by Western blotting and RT-qPCR. Results Compared with the model group, the S-Equol low-dose group had significantly decreased levels of insulin resistance (P < 0.05), the S-Equol medium-dose group had significantly decreased levels of insulin secretion (P < 0.05), and the S-Equol low-and high-dose groups had obviously decreased levels of FBG (P < 0.05); The S-Equol low-dose group had significantly decreased liver index, albumin/globulin ratio (A/G) and LDL (P < 0.05), and the S-Equol medium-dose group had significantly decreased DBIL and ALT (P < 0.05). Compared with the model group, the histological morphology of liver in the S-Equol low-, medium- and high-dose groups improved and the area of liver lipid deposition decreased obviously (P < 0.05), and the S-Equol medium-dose group and the high-dose group had reduced liver inflammation (P < 0.05). The results of Western blotting showed the expression of PPARγ and ACC in liver tissue of rats was significantly down-regulated by medium- and high-dose S-Equol intervention (P < 0.05). The results of RT-qPCR showed that S-Equol intervention significantly could reduce the transcription levels of ACC, SREBP-1, Fasn and PPARγ in liver tissue of rats (P < 0.05). Conclusion The intervention of S-Equol can reduce liver lipid deposition, lipid metabolism-related molecule expression and inflammation in T2DM rats with NAFLD, and the mechanism may be related to regulating SREBP pathway and PPARγ expression.

Objective To determine the effect of equol (Eq) intervention on high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) in ovariectomized rats and investigate its mechanism of action. Methods Sixty 5-week-old female SD rats were randomly divided into 6 groups: sham group, ovariectomized+high-fat diet (OVX +HFD) group, OVX+HFD+low-, medium-, and high-dose Eq groups [20, 40 and 80 mg/(kg·d) Eq], and OVX+ HFD+estradiol group [E2, 0.25 mg/(kg·d)], with 8 rats in each group. After 12 weeks of intervention, the rats were sacrificed and sampled. The liver index was calculated and the serum levels of blood lipid TG, TC, HDL-C, LDL-C, FFA, liver function in indicators ALT, AST, inflammatory factors TNF-α, IL-6, estrogen, fasting insulin (FINS), fasting plasma glucose (FPG) and liver contents of TG, TC, TNF-α and IL-6 were detected. HE staining was used to observe the morphology of liver tissue. qRT-PCR and Western blotting was employed to detect the mRNA expression of TNF-α and IL-6 and the protein levels of NF-κB p-p65 in the liver tissues, respectively. Results Compared with the sham group, the OVX+HFD group had significantly decreased serum estrogen level (P < 0.05) and obvious hepatic steatosis, dyslipidemia, abnormal liver function and increased levels of inflammatory factors. Eq or E2 intervention resulted in notably increased serum estrogen and HDL-C levels, and remarkably reduced serum TG, TC, HDL-C, LDL-C, FFA, ALT, AST, TNF-α and IL-6 levels (P < 0.05), down-regulated expression of NF-κB p-p65, TNF-α and IL-6 in the liver tissues (P < 0.05), and certain improvements in steatosis of liver tissues when compared with the OVX+HFD group. Conclusion Eq intervention can effectively ameliorate high-fat diet-induced NAFLD in ovariectomized rats, and the mechanism may be partially associated with its inhibition on expression of inflammatory factors.

Offshore wind power operates in a complex and harsh environment, while turbines continue to develop in the direction of large capacity and scale. Therefore, offshore wind power increasingly needs to reduce the overall operation and maintenance costs and improve the operation and control level of individual turbines and wind farms. Digital twin technology is intelligent, efficient and visual, and can provide intelligent services such as data analysis, fault diagnosis, performance evaluation and optimization suggestions for offshore wind power operation and maintenance. Relying on the digital twin five-dimensional model and its based prognostics health management method, a set of offshore wind power digital twin modeling system is deployed through the construction of data governance and maintenance fault recognition process. The system realizes the operation analysis and optimization of wind turbines, as well as the diagnosis and early warning of key equipment and field groups of wind turbines, which improves the management and control level of offshore wind power, improves the quality of operation and maintenance, optimizes the arrangement of offshore tasks, and reduces the cost of operation and maintenance. In the future, the system has great application prospects in predictive maintenance, quality improvement, efficient operation and maintenance of offshore wind power, providing support for the development of intelligent operation and maintenance of offshore wind power.

Structure determination of membrane proteins has been a long-standing challenge to understand the molecular basis of life processes. Detergents are widely used to study the structure and function of membrane proteins by various experimental methods, and the application of membrane mimetics is also a prevalent trend in the field of cryo-EM analysis. This review focuses on the widely-used detergents and corresponding properties and structures, and also discusses the growing interests in membrane mimetic systems used in cryo-EM studies, providing insights into the role of detergent alternatives in structure determination.

Objective To investigate the effect of equol (Eq) on oxidative stress and inflammatory factors in renal tissue in type 2 diabetes mellitus (T2DM) rats, and explore the possible mechanism in the process. Methods Fifty SD rats aged 6 weeks were selected, and 40 of them were given high-fat and high-sugar diet combined with intraperitoneal injection of streptozotocin to establish T2DM rat model, and finally 34 out of 40 were successfully modeled. The rats from the control group (n=10) was fed with ordinary diet, and the T2DM rats were further divided into model group (n=9), and low-(n=9), medium-(n=8) and high-dose (n=8) Eq intervention groups (gavage intervention of 20, 40 and 80 mg/kg Eq, respectively). After 12 consecutive weeks of intervention, serum creatinine (CREA), blood urea nitrogen (BUN), uric acid (UA), and levels of renal oxidative stress and inflammatory factors were detected in each group of rats. HE and PAS stainings were adopted to reveal the histological changes of renal tissue. Western blotting and RT-PCR were performed to determine the activation or inhibition of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway in renal tissue. Results As compared with the control group, the serum levels of CREA, BUN and UA were significantly increased in the T2DM model group (P < 0.05), and the levels of oxidative stress and inflammation indicators such as malondialdehyde (MDA) and TNF-α in renal tissue were remarkably elevated as well (P < 0.05). The results of HE and PAS stainings showed increased glomerular volume and mesangial matrix, thickened basement membrane of renal capsule, atrophy of renal tubules and dilation of lumen in the model group. Moreover, the protein and mRNA levels of total Nrf2 and HO-1, as well as the expression of nuclear Nrf2 protein were all greatly reduced (P < 0.05). In comparison with the model group, the serum levels of CREA, BUN and UA in the 3 Eq intervention groups were significantly decreased (P < 0.05), levels of oxidative stress and inflammation indexes in renal tissue were reduced (P < 0.05), and the damage of renal microstructure was notably improved. In addition, the protein and mRNA expression of total Nrf2 and HO-1, and nuclear Nrf2 protein level were obviously enhanced (P < 0.05). There was no statistical difference among Eq intervention groups. Conclusion Eq reduces the levels of oxidative stress and inflammatory factors in the renal tissue of T2DM rats, exerting a protective effect on the kidney, in which Nrf2/HO-1 pathway may play an important role.

In order to solve the problem of concrete shaft support in deep topsoil layer, ANSYS finite element software was used to compare and analyze the deformation and stress of hybrid fiber high-strength concrete shaft and ordinary high-strength concrete shaft when the thickness of topsoil layer varied from 200 m to 600 m. The calculation results show that: the strain of mixed fiber concrete shaft is greater than that of ordinary concrete shaft under the same stress, and the fiber can improve the brittleness of shaft; the maximum circumferential stress on shaft increases with the increase of topsoil layer thickness, and increases gradually from outside to inside along the shaft radial direction, and the circumferential strain is always compressive strain and the inner surface is greater than the outer surface. As the burial depth increases, the deformation of shaft changes from mainly axial deformation to mainly radial deformation; finally, the regression equation of the relationship between stress-strain and thickness of topsoil layer is given, which can guide the engineering practice.

This paper explored the establishment practice of a specialized Traditional Chinese Medicine(TCM) nursing outpatient service model, and summarize the characteristics of advanced practical service in TCM nursing outpatient service. By establishing the role of TCM nursing outpatient nurses, clarifying the characteristics of nursing practice activities dominated by nurse specialists of TCM nursing treatment, forming an integrated service model of specialized medical care, strengthening the quality management of TCM nursing outpatient services, and doing a good job in training and assessment of outpatient nurses' core competencies, great efforts have been made to continuously enrich the connotation of quality nursing services with TCM nursing characteristics, accelerate the establishment of TCM nursing service system, and promote the process of nursing specialization, which plays a positive role in promoting the long-term sustainable development of TCM nursing specialties, and is worthy of being performed exploration and practice by nursing managers and clinical nursing staff with specialty work experience. (本文探讨了专科中医护理门诊服务模式的建设, 总结中医护理门诊高级实践服务特点。通过确立中医护理门诊护士的角色作用, 明确中医护理治疗专科护士主导下的护理实践活动的特点, 形成专科医护一体化服务模式, 加强中医护理门诊服务质量管理, 做好出诊护士核心能力培训考核等, 不断丰富中医护理特色的优质护理服务内涵, 加快中医护理服务体系的建立, 促进护理专科化进程, 对中医护理专科的长期可持续发展有着积极的推动作用, 值得广大护理管理者和具备专科工作经验的临床护理人员去开拓和实践。)

This paper reviewed nursing management for a patient with erysipelas infection leads to sepsis combined with multiple organ dysfunction syndromes. A comprehensive treatment including anti-infective therapy, diuretic treatment, cardiac medication and circulation function treatment and related nursing management was carried out. Targeted nursing strategies including non-invasive ventilation nursing, medication care and fluid-management, fever nursing, catheter maintenance, skin care were carried out to ensure the therapeutic efficacy and symptom relieving. (本文总结1例丹毒感染致脓毒症合并多功能脏器衰竭患者的护理措施, 在给予抗感染、利尿、强心、维持循环功能治疗的同时还要加强护理, 从无创通气护理、用药监护及液体管理、发热护理、管路护理、皮肤护理等方面给予以针对性护理干预, 确保有效改善患者症状。)

In order to further clarify the sunshine stress effect of long-span and wide concrete box girder, the study selected a concrete box girder with 4×42 m span and 33.5 m width, formed the temperature field load through a field test and numerical simulation, established a numerical analysis model by using the finite element program of ANSYS, and comprehensively analyzed the longitudinal temperature stress distribution of a long-span and wide concrete box girder under sunshine. The midspan section of the first span of the box girder is selected for field stress measure, and the field measure results and numerical analysis results are compared and analyzed. The research shows that the results of field measurement and numerical simulation are basically consistent. Sunshine temperature has a great influence on long-span and wide concrete box girder, which should be paid enough attention.

Introduction. Papillary thyroid cancer (PTC) is one of the most prevalent endocrine malignancies that has increased in recent decades around the world. Although the indicator for navigating the surgical extent in PTC patients is still in debate, a key issue is how to predict that there are undetected preoperative tumors in the contralateral thyroid lobe. This study aims to find risk factors for contralateral occult papillary thyroid cancer (COPTC) to facilitate more accurate surgical decisions made for patients with PTC. Materials and Methods. In our study, we included 229 patients who underwent total thyroidectomy plus central and ipsilateral lateral lymph nodes dissection from January 1, 2019, to September 1, 2021. Univariate and multivariate logistic regression analyses were conducted to assess the association between COPTC and clinical-pathological characteristics, as well as the relation between the diameter of the occult lesions and predictors. The forest plot was plotted to visualize the prediction factors from the output of the multivariate regression analysis. A ROC curve was used to evaluate the combining potency of all the risk factors. Results. Of the 229 patients included in our study, 46 with COPTC were assigned to the case group, representing 20.1% in this study. Multifocality in one lobe (OR = 2.21, P=0.03), intact capsule (OR = 2.54, P=0.01), central lymph node metastasis (OR = 3.00, P=0.02), and Hashimoto’s thyroiditis (OR = 2.08, P = 0.04) are more prone to present contralateral occult papillary thyroid carcinoma. The ROC curve of the aggregate potency of the risk factors presents AUC = 0.701 (P

C23H15ClCuN4O3, monoclinic, P21/c (no. 14), a = 10.63346(14) Å, b = 17.99387(15) Å, c = 11.60320(16) Å, β = 116.2165(17)°, V = 1991.74(5) Å3, Z = 4, Rgt(F) = 0.0315, wRref(F2) = 0.0898, T = 293(2) K.

Accurate diagnosis of lung cancer is important for treatment decision-making. Tumor biopsy and histologic examination are the standard for determining histologic lung cancer subtypes. Liquid biopsy, particularly cell-free DNA (cfDNA), has recently shown promising results in cancer detection and classification. In this study, we investigate the potential of cfDNA methylome for the noninvasive classification of lung cancer histologic subtypes. We focused on the two most prevalent lung cancer subtypes, lung adenocarcinoma and lung squamous cell carcinoma. Using a fragment-based marker discovery approach, we identified robust subtype-specific methylation markers from tumor samples. These markers were successfully validated in independent cohorts and associated with subtype-specific transcriptional activity. Leveraging these markers, we constructed a subtype classification model using cfDNA methylation profiles, achieving an AUC of 0.808 in cross-validation and an AUC of 0.747 in the independent validation. Tumor copy-number alterations inferred from cfDNA methylome analysis revealed potential for treatment selection. In summary, our study demonstrates the potential of cfDNA methylome analysis for noninvasive lung cancer subtyping, offering insights for cancer monitoring and early detection.SignificanceThis study explores the use of cfDNA methylomes for the classification of lung cancer subtypes, vital for effective treatment. By identifying specific methylation markers in tumor tissues, we developed a robust classification model achieving high accuracy for noninvasive subtype detection. This cfDNA methylome approach offers promising avenues for early detection and monitoring.