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With the development of underwater acoustic countermeasure technology, deep learning is applied to recognize echo geometry features of underwater targets, but it faces the problem of sample scarcity. In this paper, we improved the underwater target highlight model, and established the target echo information equation of active sonar. By changing the spatial positions of target and sonar regularly, we performed the highlight image models of underwater maneuvering targets. Taking an underwater vehicle as an example, the model construction process was introduced in detail, and highlight image models of four typical acoustic scale decoys were also established, and five multi-space state highlight image data samples were generated. The eHasNet-5 convolutional classification network was designed, and the network was trained, verified and tested with the generated data. Finally, the experimental data test shows that the target highlight image generation models provide a new data augmentation method for the application of deep learning in active sonar target recognition, and the trained network by generated data has the ability to classify two-dimensional objects.

Abstract Background There are emerging clinical evidence for umbilical cord blood mononuclear cells (UCBMNCs) intervention to improve preterm complications. The first critical step in cell therapy is to obtain high-quality cells. This retrospective study aimed to investigate the quantity and quality of UCBMNCs from very preterm infants (VPIs) for the purpose of autologous cell therapy in prevention and treatment of preterm complications. Methods Very preterm infants (VPIs) born in Guangdong Women and Children Hospital from January 1, 2017, to December 8, 2022, from whom cord blood was successfully collected and separated for public or private banking, were enrolled. The UCBMNCs characters from route cord blood tests performed in cord blood bank, impact of perinatal factors on UCBMNCs, the relationship between UCBMNCs characteristics and preterm outcomes, and the correlation of UCBMNCs characteristics and peripheral blood cells in VPIs were analyzed. Results Totally, 89 VPIs underwent UCB collection and processing successfully. The median cell number post processing was 2.6 × 108. To infuse a dose of 5 × 107 cells/kg, only 3.4% of infants required a volume of more than 20 mL/kg, which exceeded the maximum safe volume limit for VPIs. However, when infusing 10 × 107 cells/kg, 25.8% of infants required a volume of more than 20 ml/kg volume. Antenatal glucocorticoids use and preeclampsia was associated with lower original UCBMNCs concentration. Both CD34+ hematopoietic stem cells (HSC) frequency and colony forming unit - granulocyte and macrophage (CFU-GM) number correlated negatively with gestational age (GA). UCBMNCs characters had no significant effect on preterm outcomes, whereas a significant positive correlation was observed between UCBMNCs concentration and total white blood cell, neutrophil, lymphocyte and PLT counts in peripheral blood. Conclusion UCBMNCs collected from VPIs was feasible for autologous cell therapy in improving preterm complications. Setting the infusion dose of 5 × 107 cells/kg guaranteed a safe infusion volume in more than 95% of the targeted infants. UCBMNCs characters did not affect preterm complications; however, the effect of UCBMNCs concentration on peripheral blood classification count should be considered when evaluating the immunomodulation of UCBMNCs transfusion.

Summary: Background: Bronchopulmonary dysplasia (BPD) is the primary severe complication of preterm birth. Severe BPD was associated with higher risks of mortality, more postnatal growth failure, long term respiratory and neurological developmental retardation. Inflammation plays a central role in alveolar simplification and dysregulated vascularization of BPD. There is no effective treatment to improve BPD severity in clinical practice. Our previous clinical study showed autologous cord blood mononuclear cells (ACBMNCs) infusion could reduce the respiratory support duration safely and potential improved BPD severity. Abundant preclinical studies have reported the immunomodulation effect as an important mechanism underlying the beneficial results of stem cell therapies in preventing and treating BPD. However, clinical studies assessing the immunomodulatory effect after stem cells therapy were rare. This study was to investigate the effect of ACBMNCs infusion soon after birth on prevention for severe BPD and long term outcomes in very preterm neonates. The immune cells and inflammatory biomarkers were detected to investigate the underlying immunomodulatory mechanisms. Methods: This single-center, prospective, investigator-initiated, non-randomized trial with blinded outcome assessment aimed to assess the effect of a single intravenous infusion of ACBMNCs in preventing severe BPD (moderate or severe BPD at 36 weeks of gestational age or discharge home) in surviving very preterm neonates less than 32 gestational weeks. Patients admitted to the Neonatal Intensive Care Unit (NICU) of Guangdong Women and Children Hospital from July 01, 2018 to January 1, 2020 were assigned to receiving a targeted dosage of 5 × 107 cells/kg ACBMNC or normal saline intravenously within 24 h after enrollment. Incidence of moderate or severe BPD in survivors were investigated as the primary short term outcome. Growth, respiratory and neurological development were assessed as long term outcomes at corrected age of 18–24 month-old. Immune cells and inflammatory biomarkers were detected for potential mechanism investigation. The trial was registered at ClinicalTrials.gov (NCT02999373). Findings: Six-two infants were enrolled, of which 29 were enrolled to intervention group, 33 to control group. Moderate or severe BPD in survivors significantly decreased in intervention group (adjusted p = 0.021). The number of patients needed to treat to gain one moderate or severe BPD-free survival was 5 (95% confidence interval: 3–20). Survivors in the intervention group had a significantly higher chance to be extubated than infants in the control group (adjusted p = 0.018). There was no statistical significant difference in total BPD incidence (adjusted p = 0.106) or mortality (p = 1.000). Incidence of developmental delay reduced in intervention group in long term follow-up (adjusted p = 0.047). Specific immune cells including proportion of T cells (p = 0.04) and CD4+ T cells in lymphocytes (p = 0.03), and CD4+ CD25+ forkhead box protein 3 (FoxP3)+ regulatory T cells in CD4+ T cells increased significantly after ACBMNCs intervention (p ＜ 0.001). Anti-inflammatory factor IL-10 was higher (p = 0.03), while pro-inflammatory factor such as TNF-a (p = 0.03) and C reactive protein (p ＜ 0.001) level was lower in intervention group than in control group after intervention. Interpretation: ACBMNCs could prevent moderate or severe BPD in surviving very premature neonates and might improve neurodevelopmental outcomes in long term. An immunomodulatory effect of MNCs contributed to the improvement of BPD severity. Funding: This work was supported by National Key R&D Program of China (2021YFC2701700), National Natural Science Foundation of China (82101817, 82171714, 8187060625), Guangzhou science and technology program (202102080104).

[Objectives] In the structural design of composite wing-in-ground (WIG) craft, there is no standard way to evaluate the strength and stability of the structure. In this paper,the calculation methods for the structural strength and stability of the composite sandwich main float and wing of composite WIG craft are studied.[Methods] The theoretical methods for evaluating the structural design load of the main float and wing structure along with the corresponding strength criteria for sandwich panel are particularly discussed,as well as methods and criteria for the general and local stability of the sandwich panel. The overall longitudinal bending strength of the main float,structural strength of the composite sandwich wing, and their general and local stability are predicted and analyzed using the proposed method.[Results] The results show that the proposed method can be used for the strength and stability analysis of the main float and wing of composite WIG craft,and the composite WIG design scheme in the calculation example meets the structural strength and stability requirements,which is then verified by a real ship test.[Conclusions] This method can provide references for the design and structural analysis of composite WIG craft.

Nowadays, the full ocean depth submersible has become a focus of world marine research, and higher requirements are proposed for the mechanical properties of the pressure hull, the core component of a submersible, in the deep-sea environment. Upgrading the materials is one of the key ways to realize this goal. Due to such superior material properties as high specific strength and corrosion resistance, certain non-metallic materials such as composites, ceramics, glass, etc. are especially suitable for the manufacture of marine equipment, and show great potential for application to the pressure hull. This paper discusses the material requirements of deep-sea submersible pressure hulls, analyzes the advantages of composites, ceramics and glass in their material properties, briefly introduces the global development and application of various non-metallic materials in submersible pressure hulls, summarizes the technical problems currently faced and discusses avenues which deserve further study in this field.

Detoxification of Semen Armeniacae Amarum requires large amounts of water, and the detoxifying wastewater contains highly toxic hydrocyanic acid. This paper aims to explore the recovery of water after the removal of cyanide from it by chemical precipitation. The results show that when the dosage of ferrous sulfate is 1.76 times of the theoretical value, the cyanide removal in water can be reduced to about 2.70×105mol/L, the hydrocyanic acid in Semen Armeniacae Amarum can be entirely removed when the treated water is recycled.