Search

To study the interaction of nanoparticles (NPs) and human serum albumin (HSA), we designed three different aminosubstituted hydrophobically cholesterol-modified pullulan NPs (CHPN NPs). Dynamic light scattering (DLS) revealed sizes of 145, 156, and 254 nm and zeta potentials of 0.835, 7.22, and 11.7 mV for CHPN1, CHPN2, and CHPN3 NPs, respectively. Isothermal titration calorimetry (ITC) revealed that the binding constants were (1.59±0.45)×105 M−1, (2.08±0.26)×104 M−1, and (2.71±0.92)×104 M−1, respectively, and HSA coverage was (1.52±0.12), (0.518±0.316), and (0.092±0.015). Fluorescence spectroscopy of HSA revealed that the fluorescence intensity was quenched by CHPN NPs, which was maintained with a long final complexation period. Circular dichroism (CD) revealed a quick decrease in the α-helix content of HSA to 39.1% after the final complexation. NPs with a more positive charge led to a greater decrease in α-helix content than occurred in other NPs, so the NP surface charge played a role in the HSA–NP interaction. After HSA binding, the surface charge was −3.66±0.12 for CHPN1, −2.65±0.06 for CHPN2 and −1.12±0.28 mV for CHPN3 NPs. The NP surface property changed because of HSA binding, which is important for NP applications.

Paeonia is recognized globally due to its ornamental value. However, the mechanisms behind the formation of distinct levels of lignification in Paeonia stems remain largely unknown. In this study, we selected three representative Paeonia species, namely P. ostii (shrub), P. lactiflora (herb), and P. × 'Hexie' (semi-shrub), to evaluate and contrast their respective anatomical structure, phytochemical composition and transcriptomic profile. Our results showed that the degree of lignin deposition on the cell wall, along with the total amount of lignin and its monomers (especially G-lignin) were higher in P. ostii stems compared to the other two species at almost all development stages except 80 days after flowering. Furthermore, we estimated a total number of unigenes of 60,238 in P. ostii, 43,563 in P. × 'Hexie', and 40,212 in P. lactiflora from stem transcriptome. We then built a co-expression network of 25 transcription factors and 21 enzyme genes involved in lignin biosynthesis and identified nine key candidate genes. The expression patterns of these genes were positively correlated with the transcription levels of PAL, C4H, 4CL2, CCR, and COMT, as well as lignin content. Moreover, the highest relative expression levels of CCR, 4CL2, and C4H were found in P. ostii. This study provides an explanation for the observed differences in lignification between woody and herbaceous Paeonia stems, and constitutes a novel reference for molecular studies of stem-specific lignification process and lignin biosynthesis that can impact the ornamental industry.

Propofol-based sedation is widely used in ERCP procedures, but adverse respiratory or cardiovascular events commonly occur. Intravenous injection of lidocaine has an analgesic effect and can reduce the requirements of fentanyl and propofol during abdominal surgery. The objective of this study was to assess the efficacy and safety of intravenous lidocaine on propofol requirements during ERCP procedures.Forty-eight patients scheduled for ERCP were randomly divided into 2 groups, the lidocaine group and the control group. All patients received .02 mg/kg midazolam and .1 μg/kg sufentanil intravenously as premedication. A bolus of propofol was applied for induction of sedation, and perfusion of propofol was applied for maintenance. Patients in the lidocaine group received a bolus of 1.5 mg/kg lidocaine intravenously followed by continuous infusion of 2 mg/kg/h, whereas the control group received the same volumes of saline solution. The primary outcome was the propofol requirement during ERCP.Compared with the control group, propofol requirements were reduced by 33.8% in the lidocaine group (212.0 ± 118.2 mg vs 320.0 ± 189.6 mg, P = .023). Involuntary movement was less common in the lidocaine group than in the control group (12.5% vs 41.7%, P = .049). In the lidocaine group, postprocedure pain and fatigue, as measured by the visual analog scale, were significantly reduced (0 [range, 0-4] vs 3 [range, 0-5], P = .005; 2 [range, 0-4] vs 5 [range, 2-8], P .001).The incidence of oxygen desaturation, hypotension, and bradycardia tended to be lower in the lidocaine group.Intravenous lidocaine can significantly decrease propofol requirements during ERCP, with higher sedation quality and endoscopist satisfaction. (Clinical trial registration number: NCT03996577.).

Commonly used tools to assess the probability of obstructive-coronary artery disease (CAD) were derived based on Caucasian cohorts, with their performance in China is still unknown. Furthermore, most were established based on non-laboratory variables, contributing to the limited predictive ability to some extent. Thus, we developed and internally validated a laboratory-based model with data from a Chinese cohort of 8963 inpatients, with suspected stable chest pain, referred to catheter-based coronary angiography (CAG) from September 2007 to April 2019, and then compared the present model's performance with the four most commonly used prediction tools, Coronary Artery Disease Consortium 1/2 Score (CAD1/2), Duke clinical score (DCS), and Diamond-Forrester score (DF). The final model was developed by random forest method, including 8 predictors derived from 70 variables. Five-fold cross-validation was performed to evaluate the model's prediction accuracy. In the external validation set, the present model showed a superior area under the receiver-operating curve (0.816), followed by DCS (0.66), CAD2 (0.61), CAD1 (0.59) and at last DF (0.58), respectively. Furthermore, the present model correctly classified 74.4% of obstructive-CAD patients as high-risk, and correctly classified more than one third of non-obstructive-CAD patients as low-risk. The present model's net reclassification improvement (NRI) showed a significant positive reclassification over CAD1 (NRI = 0.60, P < 0.001), DF (NRI = 0.59, P < 0.001), CAD2 (NRI = 0.57, P < 0.001), and DCS (NRI = 0.43, P < 0.001). Decision curve analysis demonstrated that the present model provided a larger net benefit compared with CAD1/2, DCS, and DF. In conclusion, the novel model, using 8 laboratory and non-laboratory variables, performed well in risk stratifying patients with suspected chest pain regarding the presence of obstructive-CAD in the present Chinese cohort.

Ethnopharmacological relevance Paeonia plants have been widely used as traditional Chinese medicinal materials for more than 2,000 years in the treatment of cardiovascular, extravasated blood and female genital diseases; paeoniflorin and paeonol have been implicated as the plants’ primary active ingredients. Aim of the study Previous studies have been singularly focused on the chemical constituents and content variation of the Paeonia roots in the advancement of traditional Chinese medicine, with the plants’ stems and leaves considered useless. This study aims to explore the chemical constituents, content variation, and antioxidant capacity in Paeonia stems and leaves for the future utilization of traditional Chinese medicine, given that current practices of digging and trade endanger Paeonia in the wild. Materials and methods Herein, secondary metabolites from the stems and leaves from six developmental stages of the annual growth cycle of Paeonia ostii T. Hong & J. X. Zhang, P. ‘Hexie’, and P. lactiflora Pall. were qualitatively and quantitatively analyzed via high-performance liquid chromatography with a diode array detector (HPLC-DAD) and high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). Antioxidant capacity at each stage was also evaluated by various free radical scavenging assays. Results A total of 24 metabolites were detected and identified, including 5 monoterpene glycosides, 4 tannins, 5 phenols, 9 flavonoids, and paeonol. Excepting paeonol and the phenols, the levels of each metabolite category were significantly higher in the leaves than the stems during all developmental stages. The paeoniflorin content in the P. ostii leaves was the highest during the first developmental stage and higher than the standards of the Chinese Pharmacopoeia, suggesting it to be the optimal harvesting stage for medicinal uses. Notably, the antioxidant capacity of the leaves was significantly greater than in the stems, particularly for the leaves of P. ‘Hexie’. Conclusion Our study indicates that the leaves of P. ‘Hexie’ have the potential to be a worthy medicinal substitute to Paeonia roots due to their high monoterpene glycosides, phenols, and flavonoids as well as their strong antioxidant capacity. Further, this study provides a theoretical basis for the development and utilization of non-root Paeonia plant sections as medicinal plant resources.

Chalcone synthase (CHS) is the key enzyme in the flavonoid biosynthetic pathway and has been studied in many plants, but the function of the CHS gene has not been well characterized in Paeonia ostii. In this study, we obtained a CHS homologue gene from P. ostii, which possessed the putative conserved amino acids of chalcone synthase by multiple alignment analysis and demonstrated the highest expression in developing seeds. In vitro assays of the recombinant PoCHS protein confirmed enzymatic activity using malonyl-CoA and 4-coumaroyl-CoA as substrates, and the optimal pH and reaction temperature were 7.5 and 40°C, respectively. Furthermore, ectopic over-expression of PoCHS in Arabidopsis up-regulated the expression levels of genes involved in seed development (ABI), glycolysis (PKp2, PDH-E1a, and SUS2/3), and especially fatty acid biosynthesis (BCCP2, CAC2, CDS2, FatA and FAD3). This resulted in an increased unsaturated fatty acid content, especially α-linolenic acid, in transgenic Arabidopsis seeds. In this study, we examined the functions of CHS homologue in P. ostii and demonstrated its new function in seed fatty acid biosynthesis. This article is protected by copyright. All rights reserved.

BACKGROUND: Cough is one of the shared complications after lung surgery. In this study, a prospective analysis was conducted for exploring the risk factors of persistent cough after uniportal video-assisted thoracoscopic pulmonary resection. METHODS: One hundred thirty-five patients with pulmonary nodules who underwent surgical treatment in the same surgical group from November 2019 to January 2020 were enrolled in this prospective study. The severity of cough and its impact on patients’ quality of life before and after surgery were assessed by the Mandarin Chinese version of the Leicester cough questionnaire (LCQ-MC), and postoperative cough was tested by the cough visual analog scale (VAS) and cough symptom score (CSS). Risk factors of cough after pulmonary resection (CAP) were determined by univariate and multivariate logistic regression analysis. RESULTS: The incidence of postoperative cough was 24.4% (33 of 135 patients). Univariate analysis showed that gender (female), the surgical site (upper right), the resection (lobectomy), subcarinal lymph node dissection, postoperative acid reflux, length of hospitalization contributed to the development of CAP resection. Multivariate logistic regression analysis showed that the resection (lobectomy) (OR 3.590, 95% CI: 0.637–20.300, P=0.017), subcarinal lymph node dissection (OR 4.420, 95% CI: 1.342–14.554, P=0.001), postoperative acid reflux (OR 13.55, 95% CI: 3.186–57.633, P

Commonly used tools to assess the probability of obstructive-coronary artery disease (CAD) were derived based on Caucasian cohorts, with their performance in China is still unknown. Furthermore, most were established based on non-laboratory variables, contributing to the limited predictive ability to some extent. Thus, we developed and internally validated a laboratory-based model with data from a Chinese cohort of 8963 inpatients, with suspected stable chest pain, referred to catheter-based coronary angiography (CAG) from September 2007 to April 2019, and then compared the present model's performance with the four most commonly used prediction tools, Coronary Artery Disease Consortium 1/2 Score (CAD1/2), Duke clinical score (DCS), and Diamond-Forrester score (DF). The final model was developed by random forest method, including 8 predictors derived from 70 variables. Five-fold cross-validation was performed to evaluate the model's prediction accuracy. In the external validation set, the present model showed a superior area under the receiver-operating curve (0.816), followed by DCS (0.66), CAD2 (0.61), CAD1 (0.59) and at last DF (0.58), respectively. Furthermore, the present model correctly classified 74.4% of obstructive-CAD patients as high-risk, and correctly classified more than one third of non-obstructive-CAD patients as low-risk. The present model's net reclassification improvement (NRI) showed a significant positive reclassification over CAD1 (NRI = 0.60, P0.001), DF (NRI = 0.59, P0.001), CAD2 (NRI = 0.57, P0.001), and DCS (NRI = 0.43, P0.001). Decision curve analysis demonstrated that the present model provided a larger net benefit compared with CAD1/2, DCS, and DF. In conclusion, the novel model, using 8 laboratory and non-laboratory variables, performed well in risk stratifying patients with suspected chest pain regarding the presence of obstructive-CAD in the present Chinese cohort.

Background: Memory T cells play a key role in the development of atherosclerosis (AS). This study aimed to investigate the role of AMPK signaling pathway of spleen memory T cells in the pathogenesis of AS in high-fat diet (HFD) fed mice. Methods: Mice were divided into 5 groups: normal group, AS group, AS + solvent group, AS + Compound C (AMPK inhibitor) group and AS + A-769662 (AMPK agonist) group. HFD animals were intraperitoneally treated with Compound C at 20 mg/kg thrice weekly or A-769662 at 30 mg/kg once daily for 15 weeks. Then, the degree of AS was assessed, and the proportion of memory T cell was determined by flow cytometry. Results: AS was evident in the aorta of HFD mice. The areas of plaque formation in both AS + Compound C group and AS + A-769662 group reduced as compared to the AS group and AS + solvent group. After intervention of AMPK activity, the proportion of memory T cells in the spleen reduced as compared to the AS group and AS + solvent group; the pro proportion of memory T cells in HFD groups was markedly higher than in the normal group and this increase was more evident in the AS + Compound C than in the AS + A-769662 group. Conclusions: The decreased memory T cells can improve AS, which may be related to the AMPK signaling pathway. Thus, AMPK in the memory T cells may serve as a target in the prevention and treatment of AS.

Indium doped ZnO films were grown on quartz glass substrates by radio frequency magnetron sputtering from powder targets. Indium content in the targets varied from 1at% to 9at%. In doping on the structure, optical and electrical properties of ZnO thin films were studied. X-ray diffraction shows that all the films are hexagonal wurtzite with c-axis perpendicular to the substrates. There is a positive strain in the films and it increases with indium content. All the films show a high transmittance of 86% in the visible light region. Undoped ZnO thin film exhibits a high transmittance in the near infrared region. The transmittance of indium doped ZnO thin films decreases sharply in the near infrared region, and a cut-off wavelength can be found. The lowest resistivity of 4.3×10-4 Ω·cm and the highest carrier concentration of 1.86×1021 cm-3 can be obtained from ZnO thin films with an indium content of 5at% in the target.

Tree peony seed is a novel resource of edible oil with high α-linolenic acid (ALA) content (> 40%). To assess whether pollen sources influence the seed oil quality, fatty acids of hybrids from the combination of Paeonia ostii ‘Feng Dan’ and 30 cultivars were monitored and evaluated in this study. The results indicated that the content of total fatty acid and ALA ranged from 21.23 to 29.68% and from 46.75 to 64.71%, respectively. Thirty combinations were classified into four clusters. Cluster IV including ‘Tian Xiang Jin’, ‘Zhou Ban Hong’, ‘Xiang Yang Hong’ and ‘Yu Jie’ were good pollen donors because their hybrid seeds possessed the highest contents of ALA and unsaturated fatty acids, with an average of 159.39 mg g−1 and 263.76 mg g−1, respectively. This study provides a good base for breeding new cultivars with excellent edible oil and essential fatty acid contents for food.

This research was supported by the Natural Science Foundation of China (No. 31471898) and the Key Technology Research of Oil Peony Industry in Shaanxi Province (2014HXFWYL016) and Special project to Build World‐class Disciplines Of Beijing Forestry University (2019XKJS0324). Determining the factors promoting speciation is a major task in ecological and evolutionary research and can be aided by phylogeographic analysis. The Qinling–Daba Mountains (QDM) located in central China form an important geographic barrier between southern subtropical and northern temperate regions, and exhibit complex topography, climatic, and ecological diversity. Surprisingly, few phylogeographic analyses and studies of plant speciation in this region have been conducted. To address this issue, we investigated the genetic divergence and evolutionary histories of three closely related tree peony species (Paeonia qiui, P. jishanensis, and P. rockii) endemic to the QDM. Forty populations of the three tree peony species were genotyped using 22 nuclear simple sequence repeat markers (nSSRs) and three chloroplast DNA sequences to assess genetic structure and phylogenetic relationships, supplemented by morphological characterization and ecological niche modeling (ENM). Morphological and molecular genetic analyses showed the three species to be clearly differentiated from each other. In addition, coalescent analyses using DIYABC conducted on nSSR variation indicated that the species diverged from each other in the late Pleistocene, while ecological niche modeling (ENM) suggested they occupied a larger area during the Last Glacial Maximum (LGM) than at present. The combined genetic evidence from nuclear and chloroplast DNA and the results of ENM indicate that each species persisted through the late Pleistocene in multiple refugia in the Qinling, Daba, and Taihang Mountains with divergence favored by restricted gene flow caused by geographic isolation, ecological divergence, and limited pollen and seed dispersal. Our study contributes to a growing understanding of the origin and population structure of tree peonies and provides insights into the high level of plant endemism present in the Qinling–Daba Mountains of Central China. Publisher PDF

// Qi Cao 1, * , Zheng-Dao Mao 1, * , Yu-Jia Shi 1 , Yi Chen 1 , Yun Sun 1 , Qian Zhang 1 , Lei Song 1 , Li-Ping Peng 1 1 Department of Respiratory Medicine, Affiliated Changzhou No.2 People’s Hospital, Nanjing Medical University, Changzhou 213003, P.R. China * This authors have contributed equally to this work Correspondence to: Qian Zhang, email: profzhangqian@126.com Keywords: non-small cell lung cancer, microRNA-7, FAK, ERK/MAPK signaling pathway, cell proliferation Received: April 23, 2016 Accepted: September 02, 2016 Published: October 15, 2016 ABSTRACT Objective: To investigate the effects of microRNA-7 (miR-7) on the proliferation, migration and invasion of non-small cell lung cancer NSCLC) cells by targeting FAK through ERK/MAPK signaling pathway. Methods: NSCLC tissues and adjacent normal tissues were obtained from 160 NSCLC patients after operation. NSCLC cell lines (A549, H1299 and H1355) and a normal human fetal lung fibroblast cell line (MRC-5) were obtained. NSCLC cells were assigned into miR-7 inhibitors, miR-7 mimics, blank, miR-7 mimics control, miR-7 inhibitors control, FAK siRNA and miR-7 inhibitors + FAK siRNA groups. The expressions of miR-7 and FAK mRNA in tissues and cell lines were detected by qRT-PCR and Western-Blotting. Cell proliferation, migration and invasion were detected by MTT assay, wound scratch assay and Transwell assay. Results: Compared with adjacent normal tissues, miR-7 expression was down-regulated, but the mRNA and protein expressions of FAK, ERK and MAPK were up-regulated. Compared with the blank and mimics control groups, miR-7 significantly increased but FAK, ERK and MAPK expressions decreased in miR-7 mimics and FAK siRNA groups. Cell proliferation, migration and invasion were inhibited in the miR-7 mimics and FAK siRNA groups, while opposite regarding miR-7 inhibitors group. Conclusion: The miR-7 can inhibit the activation of ERK/MAPK signaling pathway by down-regulating FAK expression, thereby suppressing the proliferation, migration and invasion of NSCLC cells. The miR-7 and its target gene FAK may be novel targets for the diagnosis and treatment of NSCLC.

Paeonia jishanensis, one of the most important ancestral species of cultivated tree peonies, is an endangered ornamental and medicinal plant endemic to China. A total of 236 individuals of P. jishanensis from 10 extant populations were analysed using 21 EST-SSR markers to assess their genetic diversity and population structure. Moderate genetic diversity levels (HE = 0.340) and high genetic differentiation among populations (FST = 0.335) were revealed. Combining the results of STRUCTURE, PCoA and neighbour-joining analyses, the 10 populations were divided into four genetic groups that were significantly related to their geographical origins, which was further supported by hierarchical AMOVA showing the highest variation of 17.9% among groups. The Mantel test showed a significant positive correlation between genetic distance and geographic distance among populations (r = 0.873, P

This work reports a kind of novel antioxidant Pickering high internal phase emulsions (HIPEs; with an oil fraction, ϕ > 0.74) stabilized by soy β-conglycinin (β-CG) and polyphenol complex nanoparticles, as outstanding protective containers for lipophilic nutraceuticals. The nanoparticles with a representative polyphenol ((-)-epigallocatechin-3-gallate; EGCG) encapsulated were fabricated through an ethanol-mediated dissociation and re-assembly process of β-CG, with greater particle size and higher surface hydrophilicity observed at higher initial EGCG concentrations. Using these co-assembled nanoparticles as sole stabilizers, a kind of HIPE gels with similar gel stiffness and microstructure, could be easily fabricated at ϕ = 0.8 and a protein concentration in the aqueous phase of 1.0 wt% using polyunsatuated fatty acid-rich flaxseed oil as the dispersed phase. These HIPE gels were extraordinarily stable against heating or long-term storage, but susceptible to freeze-thawing. The as-fabricated HIPEs showed an excellent protection to β-carotene (encapsulated in oil phase) against heating, as well as an inhibition of lipid oxidation. The oxidation protection was in an EGCG concentration dependent manner. The results would be of interest for providing a novel strategy to fabricate functionalized Pickering HIPEs as potential containers or delivery systems for lipophilic nutraceuticals.

Vascular sclerosis mostly occurs in arteries and is mainly related to anatomic structure and hemodynamics of artery. This study aimed to investigate effects of arterial blood on vein wall and explore differences of composition between arterial and venous blood. Ultrasound was used to examine the distal venous structure of arteriovenous fistula in uremia patients. Immunohistochemistry was used to study the pathology of the distal vein. Twelve patients were divided into control group and trial group. Patients received an arteriovenous fistula within 1 month in control group. Patients had undergone this surgery ≥2 years before in the trial group. Blood samples were collected from the aortic, arterial, and venous vessels of 51 patients who had taken coronary angiography and analyzed with blood routine rest, biochemical, and immunological measures to compare the differences of blood composition between artery and vein. This study was registered with the China Clinical Trial Center website under registration number ChiCTR-OOC-16008085. In the trial group, the vascular wall of distal veins of fistula were thickened and hardened. No significant differences of blood composition were found between the aortic and radial arterial blood. However, the differences in the percentages of lymphocytes and neutrophils between arterial and venous blood were significant (Pa = .0095, Pb = .01). Under smooth hemodynamic conditions, arterial blood caused hardening of the venous wall. Arterial and venous blood differed in the percentage of lymphocyte and neutrophils. This may contribute to the vascular sclerosis that is observed in arteries more often than veins.

High conductive and transparent In-doped ZnO thin films were deposited on glass substrates by radio-frequency (RF) magnetron sputtering at 250 °C. Argon gas was used as the sputtering gas, and its pressure varies from 0.1 to 4.0 Pa. The influences of deposition pressure on the structural, electrical and optical properties of the films were investigated by means of X-ray diffraction (XRD), scanning electron microscope (SEM) and Hall and transmittance measurements. The optical constant of the films was estimated from transmittance data using a nonlinear programming method. It is found that the deposition pressure affects the properties of the films significantly. The film deposited at 2.0 Pa shows the optimal crystal quality with a high transmittance of 85 % in the visible range and a low resistivity of 2.4 × 10−3 Ω·cm and can thus be used as a transparent electrode.

A feasible method was proposed to improve the vibration intensity of screen surface via application of a new type elastic screen surface with multi degree of freedom (NTESSMDF). In the NTESSMDF, the primary robs were coupled to the main screen structure with ends embedded into the elastomers, and the secondary robs were attached to adjacent two primary robs with elastic bands. The dynamic model of vibrating screen with NTESSMDF was established based on Lagrange’s equation and the equivalent stiffnesses of the elastomer and elastic band were calculated. According to numerical simulation using the 4th order Runge-Kutta method, the vibration intensity of screen surface can be enhanced substantially with an averaged acceleration amplitude increasing ratio of 72.36%. The primary robs and secondary robs vibrate inversely in steady state, which would result in the friability of materials and avoid stoppage. The experimental results validate the dynamic characteristics with acceleration amplitude rising by 62.93% on average, which demonstrates the feasibility of NTESSMDF.

As the stiffness of the elastic support varies with the physical-chemical erosion and mechanical friction, model catastrophe of a single degree-of-freedom (DOF) isolation system may occur. A 3-DOF four-point-elastic-support rigid plate (FERP) structure is presented to describe the catastrophic isolation system. Based on the newly-established structure, theoretical derivation for stiffness matrix calculation by free response (SMCbyFR) and the method of stiffness identification by stiffness matrix disassembly (SIbySMD) are proposed. By integrating the SMCbyFR and the SIbySMD and defining the stiffness assurance criterion (SAC), the procedures for stiffness identification of a FERP structure (SIFERP) are summarized. Then, a numerical example is adopted for the SIFERP validation, in which the simulated tested free response data are generated by the numerical methods, and operation for filtering noise is conducted to imitate the practical application. Results in the numerical example demonstrate the feasibility and accuracy of the developed SIFERP for stiffness identification.

A series of novel quinazolinone azoles were synthesized and characterized by NMR, IR, MS and HRMS spectra. Bioactive assays showed that some target compounds exhibited significant antimicrobial potency. Especially, nitroimidazole derivative 3a displayed comparable or even superior antibacterial efficacies (MIC = 0.03–0.05 μmol mL−1) in contrast with norfloxacin (MIC = 0.01–0.05 μmol mL−1) and chloromycin (MIC = 0.02–0.10 μmol mL−1). The preliminary interactive investigations of compound 3a with calf thymus DNA by UV-vis spectroscopy revealed that compound 3a could bind to DNA to form compound 3a–DNA complex by an intercalative mode and further block DNA replication to exert powerful antibacterial and antifungal activities. Hydrogen bonds and van der Waals forces played important roles in the association of compound 3a–HSA.

Organ transplantation is the most effective therapy for patients with end-stage disease. Preservation solutions and techniques are crucial for donor organ quality, which is directly related to morbidity and survival after transplantation. Currently, static cold storage (SCS) is the standard method for organ preservation. However, preservation time with SCS is limited as prolonged cold storage increases the risk of early graft dysfunction that contributes to chronic complications. Furthermore, the growing demand for the use of marginal donor organs requires methods for organ assessment and repair. Machine perfusion has resurfaced and dominates current research on organ preservation. It is credited to its dynamic nature and physiological-like environment. The development of more sophisticated machine perfusion techniques and better perfusates may lead to organ repair/reconditioning. This review describes the history of organ preservation, summarizes the progresses that has been made to date, and discusses future directions for organ preservation.

Paeonia ostii is a traditional ornamental and medicinal species that has attracted considerable interest for its high oil value. To facilitate the effective and rational cultivation and application of P. ostii in China, it is necessary to determine its potential spatial habitat distribution and environmental requirements. Using high-resolution environmental data for current and future climate scenarios, the potential suitable area and climatic requirements of P. ostii were modelled. Among the 11 environmental variables investigated, growing degree days, precipitation of the wettest month, mean temperature of the coldest quarter, global UV-B radiation, annual precipitation, and soil pH played major roles in determining the suitability of a habitat for the cultivation of P. ostii. Under the current environmental conditions in China, a total area of 20.31 × 105 km2 is suitable for growing P. ostii, accounting for 21.16% of the country’s total land area. Under the two future climate scenario/year combinations (i.e., representative concentration pathways [RCPs], RCP2.6 and RCP8.5 in 2050), this species would increase its suitable area at high latitudes while decrease at low latitudes. These results present valuable information and a theoretical reference point for identifying the suitable cultivation areas of P. ostii.

Soy proteins have attracted fast increasing interests to be used in food emulsion formulations, due to their good emulsifying properties. Soy β-conglycinin (SC) and glycinin are the two major storage globulins in soy proteins, with the former generally recognized to exhibit much better emulsifying properties than the latter. The current work reported that the treatment of SC with ethanol at concentrations ([E]) of 20–80% (v/v) remarkably improved its emulsification performance and interfacial stabilization, in a [E]-dependent way. The influence of the ethanol treatment on the physicochemical and structural properties of SC was characterized. As expected, the treatment led to an [E]-dependent aggregation of SC, and formation of larger particles with more compact structure. Next, the emulsifying and interfacial properties of these ethanol-treated SC samples were evaluated in terms of emulsification performance and interfacial stabilization, at two oil volume fractions of 0.2 and 0.8. In both cases, the ethanol-treated SC showed greatly improved emulsification performance and interfacial stabilization. The improvements were largely due to the facilitated formation of bridged emulsions, via the strengthened Pickering stabilization by ethanol-induced aggregation. Even at a low concentration of 0.5 wt%, the ethanol-treated SC could still produce a kind of fine emulsions (produced by microfluidization), or high internal phase emulsions with a self-supporting gel network (that could resist the disruption of 6 M urea). The results demonstrated that the ethanol pretreatment can be applied as an effective modification to remarkably improve the emulsifying properties of SC, through the strengthening of its Pickering stabilization.

A novel series of hybrids of metronidazole and quinolones as antimicrobial agents were designed and synthesized. Most prepared compounds exhibited good or even stronger antimicrobial activities in comparison with reference drugs. Furthermore, these highly active metronidazole-quinolone hybrids showed appropriate ranges of pKa, log P and aqueous solubility to pharmacokinetic behaviors and no obvious toxicity to A549 and human hepatocyte LO2 cells. Their competitive interactions with metal ions to HSA revealed that the participation of Mg(2+) ion in compound 7d-HSA association could result in a concentration increase of free compound 7d. Molecular modeling and experimental investigation of compound 7d with DNA suggested that possible antibacterial mechanism might be in relation with multiple binding sites between bioactive molecules and topo IV-DNA complex.

Based on the statics theory, a novel and feasible twice-suspended-mass method (TSMM) was proposed to deal with the seldom-studied issue of fault diagnosis for damping springs of large vibrating screen (LVS). With the static balance characteristic of the screen body/surface as well as the deformation compatibility relation of springs considered, static model of the screen surface under a certain load was established to calculate compression deformation of each spring. Accuracy of the model was validated by both an experiment based on the suspended mass method and the properties of the 3D deformation space in a numerical simulation. Furthermore, by adopting the Taylor formula and the control variate method, quantitative relationship between the change of damping spring deformation and the change of spring stiffness, defined as the deformation sensitive coefficient (DSC), was derived mathematically, from which principle of the TSMM for spring fault diagnosis is clarified. In the end, an experiment was carried out and results show that the TSMM is applicable for diagnosing the fault of single spring in a LVS.

The origin of exceptionally high photocatalytic activity of phosphorus and vanadium (P/V) co-doped TiO2 under solar-light irradiation have been investigated by using X-ray photoelectron spectroscopy (XPS) and UV–vis diffuse reflectance spectra combined with density functional theory (DFT) calculations. XPS experimental results demonstrated that a portion of the dopant P(P−3) ions were implanted into the crystalline lattice of P/V co-doped TiO2 and closed proximity V dopants at substitution farthermost Ti atom, forming [Psub.−O−V] structural units. On other hand, according to a new “noncompensated” codoping concept base on the DFT calculations, we found P substituted O (No. 1 position) and V substituted farthermost Ti (No. 7 position) were favorable in Ti-rich conditions, synchronously, the defects formation energy of P/V co-doped TiO2 was minimum only −1.383 eV, the formation energies were higher at substitutional-O and Ti site under O-rich than Ti-rich conditions. Calculated dopant energy levels of P/V co-doped TiO2 were 0 and 1.875 eV at the top of valence band and the bottom of conduction band respectively, the [Psub.−O−V] structural units were responsible for trapping the photoinduced electrons, which prolongs the life of the photoinduced charge carriers and eventually leads to a remarkable enhancement in the photocatalytic activity, the calculation results were consistent with the experiment results. These findings are expected to be crucial for understanding the roles of P and V dopants and their synergistic effect in numerous titaniamediated photocatalytic reactions.

Paeonia ostii is an important woody oil plant cultivated in China on a large scale. Its seed oil is enriched with unsaturated fatty acids and a high content of alpha-linolenic acid (ALA), which are beneficial to human health. The aim of this research is to determine the qualitative traits characteristic of P. ostii seed from various production areas in China. In this study, seed quality traits were evaluated on the basis of proximate composition, content of fatty acids, tocopherol, secondary metabolites, and the antioxidant activity of seed coat (PSC) and kernel (PSK). A high content of total fatty acids (298.89&ndash, 399.34 mg g&minus, 1), crude protein (16.91%&ndash, 22.73%), and total tocopherols (167.83&ndash, 276.70 &mu, g g&minus, 1) were obtained from PSK. Significant differences were found in the content of palmitic acids (11.31&ndash, 14.27 mg g&minus, 1), stearic acids (2.42&ndash, 4.24 mg g&minus, 1), oleic acids (111.25&ndash, 157.63 mg g&minus, 1), linoleic acids (54.39&ndash, 83.59 mg g&minus, 1), and ALA (99.85&ndash, 144.71 mg g&minus, 1) in the 11 main production areas. Eight and seventeen compounds were detected in PSC and PSK, respectively. A significantly higher content of total phenols was observed in PSC (139.49 mg g&minus, 1) compared with PSK (3.04 mg g&minus, 1), which was positively related to antioxidant activity. This study indicates that seeds of P. ostii would be a good source of valuable oil and provides a basis for seed quality evaluation for the production of edible oil and potential ALA supplements from the promising woody oil plant.

A model of vibrating device coupling two pendulums (VDP) which is highly nonlinear was put forward to conduct vibration analysis. Based on energy analysis, dynamic equations with cubic nonlinearities were established using Lagrange’s equation. In order to obtain approximate solution, multiple time scales method, one of perturbation technique, was applied. Cases of non-resonant and 1:1:2:2 internal resonant were discussed. In the non-resonant case, the validity of multiple time scales method is confirmed, comparing numerical results derived from fourth order Runge-Kutta method with analytical results derived from first order approximate expression. In the 1:1:2:2 internal resonant case, modal amplitudes of Aa1 and Ab2 increase, respectively, from 0.38 to 0.63 and from 0.19 to 0.32, while the corresponding frequencies have an increase of almost 1.6 times with changes of initial conditions, indicating the existence of typical nonlinear phenomenon. In addition, the chaotic motion is found under this condition.

Background: This study aimed to explore the effects of microRNA-21-5p (miR-21-5p) on the radiation sensitivity of non-small cell lung cancer (NSCLC) and the involvement of human MutS homolog 2 (hMSH2) One hundred fourteen NSCLC patients at stage II or III who received surgery and postoperative radiotherapy were enrolled in this study. Methods: The patients were assigned into radiation-sensitive and -insensitive groups. NSCLC A549 cells were transfected to generate control, Negative control (NC), miR-21-5p inhibitor, miR-21-5p mimic, small interfering hMSH2 (sihMSH2), miR-21-5p inhibitor + sihMSH2 and hMSH2 overexpression groups. Immunohistochemistry was performed to detect the hMSH2 expression in transfected and irradiated cells. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were performed to evaluate A549 miR-21-5p and hMSH2 expression in transfected and irradiated cells. A colony formation assay was adopted for cell survival analysis. The relationship between miR-21-5p and hMSH2 was verified by a luciferase reporter assay. Cell viability was measured by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and apoptosis was assessed by flow cytometry. NSCLC nude mouse models were established, and tumor volumes and tumor weights were recorded. Results: The radiation-sensitive group of patients exhibited lower miR-21-5p but higher hMSH2 expression than the insensitive group. For irradiated A549 cells, lower cell survival, higher apoptosis, increased miR-21-5p expression and decreased hMSH2 expression were observed at 6 and 8 Gy than at 0, 2 and 4 Gy; compared to 6 Gy, cell survival and hMSH2 expression were decreased and apoptosis and miR-21-5p expression were increased at 8 Gy. Additionally, miR-21-5p was found to target hMSH2. Compared with the control group, the cell survival rate was lower and the apoptosis rate higher in the miR-21-5p inhibitor group, whereas the opposite was observed for the miR-21-5p mimic and sihMSH2 groups. For the mouse model, decreased tumor volume and tumor weight and higher hMSH2 expression were found in the miR-21-5p inhibitor, radiation, hMSH2 overexpression, miR-21-5p inhibitor + radiation and hMSH2 overexpression + radiation groups compared with the control group. In addition, tumor volume and tumor weight were decreased and hMSH2 expression increased in the miR-21-5p inhibitor + radiation and hMSH2 overexpression + radiation groups compared with the radiation alone group. Conclusion: These findings indicate that inhibition of miR-21 can promote the radiation sensitivity of NSCLC by targeting hMSH2.

A feasible method to improve the reliability and processing efficiency of large vibrating screen via the application of an elastic screen surface with multiple attached substructures (ESSMAS) was proposed. In the ESSMAS, every screen rod, with ends embedded into elastomer, is coupled to the main screen structure in a relatively flexible manner. The theoretical analysis was conducted, which consists of establishing dynamic model promoted from the fuzzy structure theory as well as calculating for the equivalent stiffness of each attached structure. According to the numerical simulation using the NEWMARK-β integration method, this assembling pattern significantly leads to the screen surface/rod having larger vibration intensity than that of the corresponding position on screen structure, which specifically, with an averaged acceleration amplitude increasing ratio of 11.37% in theoretical analysis and 20.27% in experimental test. The experimental results, within a tolerant error, also confirm the established model and demonstrate the feasibility of ESSMAS.

A new concept of banana vibrating screen which has the same effect as traditional banana vibrating screen in a new way was put forward. The dynamic model of vibrating screen was established and its working principle was analyzed when the action line of the exciting force did not act through the centroid of screen box. Moreover, the dynamic differential equations of centroid and screen surface were obtained. The motions of centroid and screen surface were simulated with actual parameters of the design example in Matlab/Simulink. The results show that not only the amplitude has a significant decrease from 9.38 to 4.10 mm, but also the throwing index and vibrating direction angle have a significant decrease from 10.49 to 4.59, and from 58.10° to 33.29°, respectively, along the screen surface, which indicates that motion characteristics of vibrating screen are consistent with those of traditional banana vibrating screen only by means of a single angle of screen surface. What’s more, such banana vibrating screen of variable linear trajectory with greater processing capacity could be obtained by adjusting the relative position of force center and the centroid of screen box properly.

To investigate the regulatory role of RhoA/Rho kinase in the effects of fluvastatin (Flu) on the tumor necrosis factor-α (TNF-α) induced expression of tissue factor (TF) in human umbilical vein endothelial cells (HUVECs) which may provide basis for the prevention of arterial thrombosis, HUVECs in logarithmic phase were divided into TNF-α group, Flu group and TNF-α+Flu group. enzyme-linked immunosorbent assay (ELISA), real time polymerase chain reaction (RT-PCR) and western blot assay were employed to detect the content of TF, messenger ribonucleic acid (mRNA) expression of TF and protein expressions of TF and activated RhoA, respectively. Then, Angiotensin II (Ang II) and Y27632 were used to treat these cells which were then divided into control group, TNF-α+Ang II group, AngII group, TNF-α group and TNF-α+Y27632 group. Western blot assay was performed to detect the protein expression of TF. After TNF-α treatment, the content and mRNA and protein expressions of TF were markedly increased when compared with the control group (P

The Ce3+/TiO2 complex membrane made from Ce3+on the TiO2 framework with the activity of photocatalysis were prepared. The degradation behavior of the best mass ratio of Ce3+/TiO2 complex membrane , and the influences of the diameters of particles of Ce3+/TiO2 complex membrane on the degradation of the harmful gases under 254nm and 365nm ultraviolet light or natural light in room were investigated. AFM result showed that the Ce3+/TiO2 complex membrane were composed of nanoparticles with 10nm~50nm diameters of particles. The experimental results revealed that the smaller the nanoparticles in Ce3+/TiO2 complex membrane, the greater the degradation degree was. The best mole ratio of Ce3+ and TiO2 was 5% and the best mass ratio of Ce3+/TiO2 and formaldehyde were 7.06 under 254nm and 365nm ultraviolet and 23.55 under the natural light in room after 24h when the degradation percents of formaldehyde were more than 90%. Same as above conditions ammonia were 8.76 and 25.79 and benzene were 15.65 and 35.32. The degradation percents of Ce3+/TiO2 complex membrane to harmful gases were higher about 3 times under 254nm and 365nm ultraviolet than under the natural light in room.

Foxp3(+) T cells regulate inflammation and tumorigenesis. However, little is known about the role of different subsets of Foxp3(+) T cells in malignant or tuberculous hydrothorax.The numbers of CD4(+)CD25(+)Foxp3(+), CD4(+)CD25(-)Foxp3(+) T cells and the levels of some inflammatory cytokines in patients with tuberculous hydrothorax, malignant hydrothorax, and healthy controls (HCs) were examined by flow cytometry and ELISA. The potential association between the numbers of different subsets of Foxp3 + T cells and the values of clinical measures were analyzed.The numbers of peripheral blood CD4(+)CD25(+)Foxp3(+) T cells were greater in malignant hydrothorax patients than in HCs, but fewer than those of hydrothorax in patients. The percentages of circulating IL-10(+) or LAP(+) CD4(+)CD25(+)Foxp3(+) T cells were higher than in the hydrothorax in patients with malignant hydrothorax. The numbers of circulating CD4(+)CD25(-)Foxp3(+) T cells were significantly fewer in patients with tuberculous hydrothorax than in HCs, and both the numbers of circulating CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) T cells were significantly fewer than in the hydrothorax in patients. Significantly higher percentages of circulating IL-10(+) or LAP(+) CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) T cells were detected in tuberculous hydrothorax patients. The numbers of CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) T cells were associated with hydrothorax adenosine deaminase (ADA) levels in tuberculous hydrothorax patients, while CD4(+)CD25(+)Foxp3(+) T cells were associated with carcino-embryonic antigen (CEA) in malignant hydrothorax patients. The concentrations of serum IL-6 and TGF-β in the patients were significantly higher than that in the HCs, but lower than that in the corresponding hydrothorax. A similar pattern of IL-10 was observed in different groups, except that there was no significant difference in the levels of serum IL-10 between the tuberculous hydrothorax patients and HCs.CD4(+)CD25(-)Foxp3(+) T cells, which have lower inhibitory function than CD4(+)CD25(+)Foxp3(+) T cells, may play a role in tuberculous hydrothorax.

An ultra-high performance liquid chromatography tandem mass spectrometry (U-HPLC-MS/MS) method was developed and validated to determine liensinine and isoliensinine in rat plasma simultaneously. Plasma samples were prepared using protein precipitation with acetonitrile. The two analytes and the internal standard pirfenidone were separated on an Acquity U-HPLC BEH C18 column with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40mL/min. Both liensinine and isoliensinine were eluted at 0.63 and 0.82min, respectively. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 611.6 → 206.2 for liensinine and m/z 611.4 → 192.2 for isoliensinine. The linearity of this method was found to be within the concentration range of 5-700ng/mL for liensinine and isoliensinine in rat plasma. The lower limits of quantification (LLOQ) were all 5ng/mL for liensinine and isoliensinine. The relative standard deviations (RSD) of intra and inter precision were less than 10% for both liensinine and isoliensinine. The method was also successfully applied to the pharmacokinetic study of liensinine and isoliensinine in rats.

To investigate the effect of pregnancy-associated plasma protein-A (PAPP-A) on human umbilical vein endothelial cell (HUVEC) contraction and relaxation function. We also investigated some effects of pregnancy serum, which was voluntarily provided by women who were 9 months pregnant, on PAPP-A function and endothelial cell function.HUVECs were cultured in vitro with PAPP-A at 0, 50, 100, and 200 ng/mL for 0, 12, 24, 48 hours. HUVECs were also pretreated for 12 hours with pregnancy serum before in vitro culture in the presence or absence of PAPP-A (50 ng/mL). Nitric oxide (NO) levels in cell supernatants were assessed using spectrophotometry. Endothelin-1 (ET-1) levels were determined using immunohistochemistry assays and quantified by mean optical density (MOD).The NO levels of HUVECs in the PAPP-A groups were significantly lower compared to the control group (P0.05), whereas ET-1 levels in PAPP-A HUVECs were significantly higher compared to the control group (P0.05.) Additionally, these effects were dose-dependent. The NO levels of HUVECs in the PAPP-A groups with pregnancy serum were higher compared to the control group (P0.05). ET-1 levels in PAPP-A HUVECs with pregnancy serum were lower than in the control group (P0.05).PAPP-A may affect HUVEC contraction and relaxation by reducing NO secretion and increasing ET-1 levels. In addition, pregnancy serum may affect PAPP-A function on vascular endothelial cells.

Recent studies have demonstrated that increased RhoA/Rho-kinase activity and reduced nitric oxide activity have the necessary machinery to induce cirrhosis. However, it is unclear whether this regulates the functions of hepatic stellate cells (HSCs). In this study, we used sodium ferulate (SF) in a cirrhotic rat model and examined its roles in regulating RhoA activation in HSCs and the subsequent effects on contraction of HSCs. Bile duct ligation method was used to induce cirrhosis in rats. Intrahepatic resistance was investigated in in situ perfused livers. Hepatic RhoA, Rho-kinase and eNOS expressions were studied by RT-PCR and Western blot. RhoA pull-down assay and collagen gel contraction assay of HSCs were performed by incubation with SF in the absence or presence of GGPP. We showed that in cirrhotic liver, SF can efficiently affect RhoA activation via lowering the synthesis of GGPP in HSCs. These actions effectively reduced basal intrahepatic resistance in cirrhotic rats. Our study further suggested that SF effectively decreased Rho-kinase activity and increased activity of eNOS at both the mRNA and protein levels. SF treatment of HSCs reduced RhoA GTP without affecting the total RhoA protein level, and GGPP had the ability to block SF-induced protein expression. Furthermore, SF inhibited the contraction of activated HSCs and this inhibition was efficiently reversed by addition of GGPP. SF inhibits hepatic RhoA/Rho-kinase signaling and activates the NO/PKG pathway in cirrhotic rats. This may serve as a mechanism for reducing the contraction of activated HSCs upon SF treatment.

This paper aims to investigate the influence of single-nucleotide polymorphisms (SNPs) in the receptor of activator of nuclear factor kappaB ligand (RANKL) gene (TNFSF11) and osteoprotegerin (OPG) gene (TNFRSF11B) on bone and joint injury in patients with rheumatoid arthritis (RA).Two hundred RA patients and 201 matched controls were analysed by case-control design, and their samples were genotyped. Bone mineral density (BMD) and serum OPG and RANKL levels were measured. Clinical and laboratory parameters were recorded, and the radiographic changes in both hands of RA were evaluated by Sharp's method.Our results showed no significant differences in the distribution frequency of the alleles and genotypes of TNFRSF11B (rs2073618 and rs3102735) and TNFSF11 (rs2277438) between the RA group and controls (p0.05). Compared to patients with TNFSF11 (rs2277438) AA or GG genotype, RA with TNFSF11 (rs2277438) AG genotype had significantly decreased BMD values at lumbar spine 3, lumbar spine 4, lumbar spine 2-4 (p0.05-0.01), and apparently elevated Sharp scores (p0.05), respectively. The RA group showed significantly higher serum levels of RANKL, RANKL/OPG ratio and a lower serum level of OPG than that of the controls (p0.05-0.0001). RA patients with RANKL-rs2277438 heterozygotic genotype (AG) had significantly increased serum levels of RANKL (p0.05), compared to homozygotic genotype (AA or GG).These results indicate that SNP of TNFRSF11B (rs2073618 and rs3102735) and TNFSF11 (rs2277438) may not be susceptibility factors for RA in Chinese Han population. SNP of TNFSF11 (rs2277438) may have an important influence on bone and joint injury in RA.

To study the influence of survivin mutant-T34A (survivin(T34A)) and survivin deletant-N-terminal 8 amino acids residues (survivin(N-8AA)) on the cell cycle distribution and chemosensitivity in human ovarian cancer HO-8910 cells for explorating the roles of modified survivin-mediated apoptosis induced by chemotherapeutic agents and possible signaling pathways involved.pcDNA3.1 plasmid contained wild-type, survivin(T34A) and survivin(N-8AA) genes were transfected into HO-8910 cells, respectively, the control groups were HO-8910 cells transfected with pcDNA3.1 plasmids. The expression of mRNA was examined by reverse transcription(RT) PCR and identified by DNA sequencing; the cell cycles were determined by flow cytometer analysis (FCM); the growth inhibitions rate of cisplatin (DDP), paclitaxel (PTX) and LY294002 on the transfected cells were determined using methyl thiazolyl tetrazolium (MTT) assay.(1) The RT-PCR procedures and genome sequences showed that the survivin mRNA were expressed stable in the transfected HO-8910 cells. (2) There was lower percent of G(0)/G(1) phase cells in SN-HO-8910 cells than that in PC-HO-8910 cells (44.72% vs. 49.64%, P0.05); while higher percentage of G(2)/M phase and S phase cells (1.06% and 54.22% vs. 0.56% and 49.80%, P0.05). There was lower the G(2)/M phase and S phase cells in M-HO-8910 cells 0.16% and 36.33%, than that in PC-HO-8910 cells (P0.05); while higher percentage of G(0)/G(1) phase cells (63.51%, P0.05). G(0)/G(1), G(2)/M and S phase cells in Sur-HO-8910 cells were 54.46%, 0.62% and 44.92%, and there were not significantly difference (P0.05), compared to those in PC-HO-8910 cells. (3) The inhibitory concentration (IC(50)) of DDP and PTX were higher in Sur-HO-8910 cells than those in control cells [(20.4 ± 6.1) vs. (14.4 ± 3.9) µmol/L, (36.7 ± 4.0) vs. (28.6 ± 3.6) µmol/L; all P0.05]. The IC(50) of DDP and LY294002 in SN-HO-8910 cells were lower than those in control cells [(7.6 ± 1.0) vs. (14.4 ± 3.9) µmol/L, (13.2 ± 4.0) vs. (41.0 ± 7.9) µmol/L; all P0.01]. The IC(50) of PTX [(37.9 ± 4.8) µmol/L] in SN-HO-8910 cells were higher than that in control cells (P0.05). The IC(50) of DDP in M-HO-8910 cells [(9.9 ± 1.2) µmol/L] were lower than that in control cells (P0.05), and the IC(50) of LY294002 in M-HO-8910 cells [(66.9 ± 4.8) µmol/L] higher than that in control cells (P0.01).The changes of cells cycle distribution caused by survivin(T34A) or survivin(N-8AA) enhanced the G(2)/M cell cycle-dependent chemosensitivity of PTX. Compared to survivin(T34A), survivin(N-8AA) preferentially to mediate the cytotoxicity of DDP and LY294002, suggesting that it may be related to the cell cycle-dependence of survivin function and to blockage of the formation of its active dimer.

To compare clinical results of two methods for the treatment of femoral neck fracture, which are cannulated screw fixation combined with percutaneous autogenous bone marrow grafting, and simple cannulated screw fixation. To investigate the curative effects of cannulated screw fixation combined with percutaneous autogenous bone marrow garfting to promote fracture healing and reducing femoral neck necrosis.The clinical data of 60 cases, which were enrolled from December 2000 to December 2008 consecutively in our hospital, were analyzed retrospectively. Thirty patients with femoral neck fractures were treated with cannulated screw fixation and percutaneous autogenous bone marrow grafting. There were 20 males and 10 females, ranging in ages from 18 to 89 years,with an average of (52.3 +/- 0.2) years. There were 13 patients with traffic accident, 3 patients with falling injuries and 14 patients with tumble. Based on the Garden classification for femoral neck fractures, 1 patient was type I, 6 patients were type II, 12 patients were type III and 11 patients were type IV. Among 30 patients in the control group, 16 patients were male and 14 patients were female, ranging in age from 18 to 91 years, with an average of (51.9 +/- 0.1) years. Twelve patients injured with traffic accident, 1 patient with falling injuries and 17 patients with tumble. Based on the Garden classification for femoral neck fractures, 5 patients were type I, 2 patients were type II,15 patients were type III, and 8 patients were type IV. Patients in the control group were treated with cannulated screw fixation only. All the patients were followed up for 2 years after operation. The fracture healing and complications were evaluated and compared between the two groups.The average healing time was (7.1 +/- 1.2) months in the observing group and (8.0 +/- 1.4) months in the control group. The healing of femoral neck fracture occurred in 29 cases in observing group while in 24 cases in the control group contrast to femoral head necrosis occurred in 1 case in the observing group while in 6 cases in the control group. According to Harris scoring system, the good and excellent rate of the two groups had statistical difference (P0.05).Cannulated screw fixation and percutaneous autogenous bone marrow grafting is a more efficient method for accelerating healing of femoral neck fractures and reducing femoral head necrosis.