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Non-platinum-metal complexes show great potential as anticancer agents. Herein, a series of dithiocarbazate non-Pt-metal complexes, including [Fe

This retrospective study appraised the preventive effect of statin after carotid artery stenting (CAS). Records were extracted for 100 patients with CAS surgery indicator, aged between 20 and 75 years old, and treated for statin. The cohort study included treatment group (statin and routine treatment) and control group (routine treatment), each group 50 patients. Outcomes consisted of degree of nerve defect (as measured by National Institute of Health Stroke Scale), lipid profiles (mg/dL), and CAS complications within 30 days after surgery. After treatment, there were no significant differences in National Institute of Health Stroke Scale, lipid profiles, and mortality rate between 2 groups. However, significant differences in total cholesterol (mg/dL, P = .03), low-density lipoprotein (mg/dL, P = .01), transient ischemic attack (P = .03), ischemic stroke (P = .04), and cardiac complications (P = .03) were identified within 30 days after CAS between 2 groups. The results of this study showed that prior statin treatment may be effective for the prevention of CAS complications.

Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability. The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch. Regardless of the extraction temperature, the xylose chain was the main component (35%–40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior. Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability. This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.

The complete mitochondrial genome of Ampittia dioscorides (Lepidoptera: Hesperiidae) was determined. The sequenced genome is a circular molecule of 15313 bp, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and an A + T-rich region. The gene arrangements and transcribing directions are identical to those in most of the reported lepidopteran mitogenomes. The base composition of the whole genome and genes or regions are also similar to those in other lepidopteran species. All the PCGs are initiated by typical ATN codons; the exception being COI, which begins with a CGA codon. Eight genes (ND2, ATPase8, ATPase6, COIII, ND5, ND4L, ND6, and Cytb) end with a TAA stop codon, and two genes (ND1 and ND3) end with TAG. The remaining three genes (COI and COII, which end with TA-, and ND4, which ends with T-) have incomplete stop codons. All tRNAs have the typical clover-leaf structure of mitochondrial tRNAs, with the exception of tRNASer(AGY). On the basis of the concatenated nucleotide and amino acid sequences of the 13 PCGs and wingless gene of 22 butterfly species, maximum parsimony (MP) and Bayesian inference (BI) trees were constructed, respectively. Both MP and BI trees had the same topological structure: ((((Nymphalidae + Danaidae) + Lycaenidae) + Pieridae) + Papilionidae) + Hesperiidae). The results provide support for Hesperiidae as a superfamily-level taxon.

The oil recovery of the traditional process of extracting sesame oil by aqueous method could be improved by adding enzyme. Sesame oil was extracted under aqueous extraction conditions using three kinds of enzymes, which were papain, trypsin and cellulase. The optimal conditions were pH 7.0, 50 °C and extraction time 3 h with constant shaking at 80 rpm. The enzyme dosage and the enzyme proportion were researched by orthogonal experiment. The result indicated that the enzymes significantly improved the oil recovery. When the enzyme hydrolysis was performed with complex enzyme of papain 6,000 U, trypsin 400 U and cellulase 250 U/g oilseed, higher oil recovery (87.58 %) was achieved than the traditional aqueous extraction processing, and the oil was extremely fragrant. Moreover, the clear oil could fall directly from the centrifuge tube which greatly reduced the difficulty of separating oil.

The aqueous extracting method of sesame oil was studied in this paper．On basis of the single-factor experiment, the orthogonal trial was used in optimization of technological parameters. The optimal conditions for sesame oil extraction were solid to water ratio of 0.8 g/ml (v/w), 70°Cand pH 5.0, under which the extraction rate reached 82.49％．

Recovery of sweet potato protein materials from the starch waste was studied in the paper. Ultrafiltration followed with alkaline extraction and acid precipitation by adjusting pH through lactic fermentation, had the highest extraction rate (40.81%). While ultrafiltration followed with alkaline extraction and acid precipitation by adjusting pH with HCl yielded the protein material with the highest protein content (73.68 %), these two procedures provide good alternatives for different purpose.

Based on the viscoelasticity theory and the data of creep test, Burgers model was established, which was used to study the viscoelastic property of SBR asphalt mixtures, and the viscoelastic constitutive relation was obtained. Using the finite element method, the temperature stresses field was calculated under the environmental conditions and the thermal stresses of SBR modified asphalt pavement was given at the last part of this paper. The study indicated that SBR modified asphalt mixtures have the advantage over common asphalt mixture in low-temperature performance.

The complete mitochondrial genome sequence of Goniurosaurus luii has been determined in the present paper. The genome was 16,519 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). Its gene composition and order was similar to most other Squamate reptiles. The overall base composition of the genome in descending order was 34.11% A, 26. 01% C, 27.43% T, and 12.45% G, with a slight AT bias of 61.54%. CR was located between the tRNA-Pro and tRNA-Phe genes and was 1147 bp in length, some tandem repeat sequences and conserved elements (CSB2-3) were found in the control region.

The complete mitochondrial genome sequence of Papilio bianor was determined in the present paper. The complete mtDNA from P. bianor was 15,358 base pairs in length and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and a control region. The P. bianor genes were in the same order and orientation as the completely sequenced mitogenomes of other lepidopteran species. To determine the phylogentic position of P. bianor with related species within Papilionidae, the Bayesian phylogenetic tree was reconstructed with the concatenated nucleotide dataset of the 13 protein-coding genes. The phylogenetic trees confirmed that P. bianor and four species of Papilionidae clustered into a clade, and shared a close relationship with Papilio maraho. Meanwhile, the molecular phylogenetic trees also confirmed that Papilionidae is a monophyletic group, and Pieridae is closely related with Lycaenidae and Nymphalidae.

The complete sequence mitochondrial genome of Takydromus sexlineatus was determined using long PCR and conserved primers walking approaches. The genome was 18,943 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of T. sexlineatus were similar to most other squamate reptiles. All protein-coding genes begin with ATG as initiation codon except COI using GTG. Seven genes (ATP8. ND4L. ND5. Cytb. ND1. COI and ND6) ended with TAA, TAG, AGGA and AGA stop codon, the remaining 6 genes had incomplete stop codons T/TA. The overall base composition of the genome in descending order was 31.48% A, 24.67% C, 30.79% T and 13.05% G, with a slight A + T bias of 62.27%. CR is located between the tRNA-Pro and tRNA-Phe genes and is 3562 bp in length, some tandem repeat sequences, conserved elements (CSB1-3) and termination associated sequences (TAS1-3) were found in the control region.

The complete sequence mitochondrial genome of Papilio polytes was determined using long PCR and conserved primers walking approaches. The genome was 15,260 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of P. polytes were similar to other lepidopteran species. All protein-coding genes begin with ATG and ATT as initiation codon except COI using CGA. 8 genes (ATP8, ATP6, ND3, ND5, ND4L, ND6, Cytb and ND1) ended with TAA and TAG stop codon, the remaining five genes had incomplete stop codon T. The overall base composition of the genome in descending order was 39.51% A, 11.86% C, 40.75% T and 7.88% G, with a A + T bias of 80.26%. CR is located between the 12S rRNA and tRNA-Met genes and is 439 bp in length, with an AT content of 83.37%.

Through pharmacological combined with laser scanning confocal microscope (LSCM) and spectrophotography to study the role of Ca2+ and NO in signaling during Vicia faba L. stomatal movement response to ethylene (Eth). The results showed that treatment with ethephon (0.004%, 0.04%, 0.4%) resulted in a time- and dose-dependent stomatal closure under light. NO scavenger cPTIO, nitrate reductase inhibitor NaN3, or extracellular Ca2+ chelation EGTA reduced ethylene-induced stomatal closure. Moreover, ethylene was shown to enhance nitric oxide levels and, corresponding, nitrate reductase activity. Inhibition of the nitrate reductase diminished ethylene-induced NO production in both stomatal guard cell and leaf. Finally, ethylene-induced NO levels and nitrate reductase activity decreased when Ca2+ was compromised. On the basis of biochemical and pharmacological experimental results, we can conclude that Ca2+ and NO were involved in the signal transduction pathway of ethylene induced stomatal closure. Nitrate reductase-derived NO may represents a novel downstream component of Ca2+ signaling cascade during ethylene-induced stomatal movement in Vicia faba L.

The effects of nitric oxide (NO) and ethylene on Vicia faba L. stomatal movement were studied. The results showed that NO donor SNP (sodium nitroprusside) 10 micromol/L and ethylene 0.04% could induce stomatal closure distinctly and they could promote stomatal closure when treated together. When treated with AVG (an inhibitor of ethylene synthesis), c-PTIO (a specific scavenger of NO) and NaN(3) (an inhibitor of NR), the effects of NO- and ethylene-induced stomatal closure were inhibited but the inhibitor of nitric oxide synthase (NOS) had little effect. We presumed that there was coordinative effect between NO and ethylene in regulation of stomatal closure; ethylene could induce stomatal closure by regulating the production of nitrate reductase (NR)-dependent NO.

The ingestion of lactobacilli is of great importance for the probiotic effect of host gut Peyer's patches (PPs) macrophages. The present study is in time focus on the investigation of the factors determining the ingestion of lactobacilli by PPs macrophages. Physicochemical properties of cell surface and adhesive property of nine Lactobacillus strains were examined in the present work. The association of the bacteria with PPs macrophage was checked with macrophage monolayers on coverslips. The influence of lactobacilli on macrophages phagocytic capacity was also investigated with a neutral red uptake assay in vitro. The results show that the macrophages could ingest lactobacilli in a strain dependent manner, and the most ingested strain is L. plantarum Lp6 compared to other tested strains, which displayed strain specific enhancement on the phagocytic activity of PPs macrophages. And there is no correlation between the physicochemical or adhesive properties of the cell surface and the ingestion. The association of L. plantarum Lp6 with PPs macrophage could be decreased by Protease K treatment. Surface proteins of L. plantarum Lp6 could promote the ingestion of fluorescent latex beads by PPs macrophages. In conclusion, the hydrophobicity of the cell surface might not be the key factor determining the association of lactobacilli with PPs macrophages. Cell surface proteins are the media for the binding L. plantarum Lp6 to macrophages.

Here, we sequenced the complete mitochondrial genome of Spilornis cheela (Falconiformes, Accipitridae), which is considered as endemic raptor species and listed in the second category of National Key Protected Wild Animals in China. The genome is 18,291 bp in size. Its gene arrangement pattern was identical with that of Spizaetus alboniger. We compared the mitochondrial genome of S. cheela with that of S. alboniger. Nucleotide sequence similarity between the two whole mitochondrial genomes was 84.34%, and the relatively low similarity seems to indicate that the two species are distinctly separated on the species level. The information on the mitochondrial genome comparison of the two species is discussed in detail in this paper.

In order to characterize a specific extracellular 21-kDa calmodulin-binding protein (named: ECBP21) from Angelica dahurica L. suspension-cultured cells, the cDNA coding for the protein has been cloned. Here, Southern blot analysis shows that there are at least two copies of ECBP21 gene in Angelica genome. Using truncated versions of ECBP21 and synthetic peptide in CaM binding assays, we mapped the calmodulin-binding domain to a 16-amino acid stretch (residues 200–215) at the C-terminal region. The ECBP21 was localized in the cell wall area by the immunogold electron microscopy and by GFP labeling method. These results define ECBP21 as a kind of an extracellular calmodulin-binding protein (CaMBP). Furthermore, using Northern blot analysis, we examined the expression dynamics of ecbp21 during the incubation of Angelica suspension-cultured cells and the treatments with some growth regulators. The above studies further provide the molecular evidence for the existence of the gene coding for extracellular CaMBPs and imply a possible role for ECBP21.

Pharmacological, laser scanning confocal microscopic (LSCM), real-time PCR and spectrophotographic approaches are used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement response to ethylene in Arabidopsis thaliana. In the present study, inhibitors of H2S synthesis were found to block ethylene-induced stomatal closure of Arabidopsis. Treatment with ethylene induced H2S generation and increased L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves. Quantitative PCR analysis showed AtL-CDes and AtD-CDes transcripts were induced by ethylene. It is suggested that ethylene-induced H2S levels and L-/D-cysteine desulfhydrase activity decreased when NO was compromised. The data clearly show that ethylene was able to induce H2S generation and stomatal closure in Atnoa1 plants, but failed in the Atnia1,nia2 mutant. Inhibitors of H2S synthesis had no effect on ethylene-induced NO accumulation and nitrate reductase (NR) activity in guard cells or leaves of Arabidopsis, whereas ethylene was able to induce NO synthesis. Therefore, we conclude that H2S and NO are involved in the signal transduction pathway of ethylene-induced stomatal closure. In Arabidopsis, H2S may represent a novel downstream indicator of NO during ethylene-induced stomatal movement.